M1_20170612_1833_dsRNA_U3_faster.rtdc
Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_16.51.57
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}dclab-tdms2rtdc
{"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "64a67fe3e96bc54bdaf83c2d837d3eff",
"name": "M1_20170612_1833_dsRNA_U3_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "14:52:18"
}
}
FG_Config.mcf
[Kamerasektion]ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.7867
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.45581e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 0 mean_circ: 0.919121
Ran for 550 frames Cell Events: 1 mean_circ: 0.819367
Ran for 1050 frames Cell Events: 10 mean_circ: 0.902684
Ran for 2050 frames Cell Events: 23 mean_circ: 0.902482
Ran for 2550 frames Cell Events: 33 mean_circ: 0.897675
Ran for 3550 frames Cell Events: 47 mean_circ: 0.903607
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Ran for 368050 frames Cell Events: 5506 mean_circ: 0.904703
Ran for 368550 frames Cell Events: 5515 mean_circ: 0.904708
Ran for 369050 frames Cell Events: 5522 mean_circ: 0.904764
Ran for 369550 frames Cell Events: 5533 mean_circ: 0.904745
Ran for 370550 frames Cell Events: 5547 mean_circ: 0.904791
Ran for 371050 frames Cell Events: 5553 mean_circ: 0.904794
Ran for 372050 frames Cell Events: 5572 mean_circ: 0.904734
Ran for 372550 frames Cell Events: 5581 mean_circ: 0.904748
Ran for 373050 frames Cell Events: 5597 mean_circ: 0.904752
Ran for 373550 frames Cell Events: 5606 mean_circ: 0.904807
Ran for 374050 frames Cell Events: 5611 mean_circ: 0.904801
Ran for 374550 frames Cell Events: 5616 mean_circ: 0.90476
Ran for 375550 frames Cell Events: 5640 mean_circ: 0.904794
Ran for 376050 frames Cell Events: 5647 mean_circ: 0.904852
Ran for 376550 frames Cell Events: 5653 mean_circ: 0.904894
Ran for 377050 frames Cell Events: 5666 mean_circ: 0.904944
Ran for 377550 frames Cell Events: 5672 mean_circ: 0.904955
Ran for 378050 frames Cell Events: 5678 mean_circ: 0.904956
Ran for 378550 frames Cell Events: 5684 mean_circ: 0.904989
Ran for 379050 frames Cell Events: 5687 mean_circ: 0.905021
Ran for 379550 frames Cell Events: 5691 mean_circ: 0.905
Ran for 380050 frames Cell Events: 5695 mean_circ: 0.905011
Ran for 381050 frames Cell Events: 5711 mean_circ: 0.905068
Ran for 381550 frames Cell Events: 5722 mean_circ: 0.905067
Ran for 382050 frames Cell Events: 5730 mean_circ: 0.905047
Ran for 382550 frames Cell Events: 5740 mean_circ: 0.905014
Ran for 383550 frames Cell Events: 5759 mean_circ: 0.905081
Ran for 384050 frames Cell Events: 5771 mean_circ: 0.905141
Ran for 384550 frames Cell Events: 5778 mean_circ: 0.90515
Ran for 385050 frames Cell Events: 5787 mean_circ: 0.905161
Ran for 386050 frames Cell Events: 5801 mean_circ: 0.905197
Ran for 386550 frames Cell Events: 5812 mean_circ: 0.90519
Ran for 387550 frames Cell Events: 5824 mean_circ: 0.905197
Ran for 388050 frames Cell Events: 5833 mean_circ: 0.905231
Ran for 388550 frames Cell Events: 5837 mean_circ: 0.90521
Ran for 389050 frames Cell Events: 5849 mean_circ: 0.905154
Ran for 389550 frames Cell Events: 5857 mean_circ: 0.905099
Ran for 390550 frames Cell Events: 5883 mean_circ: 0.905125
Ran for 391050 frames Cell Events: 5893 mean_circ: 0.905152
Ran for 392050 frames Cell Events: 5910 mean_circ: 0.90515
Ran for 393050 frames Cell Events: 5925 mean_circ: 0.905174
Ran for 394050 frames Cell Events: 5940 mean_circ: 0.905188
Ran for 394550 frames Cell Events: 5945 mean_circ: 0.905202
Ran for 396050 frames Cell Events: 5974 mean_circ: 0.905215
Ran for 396550 frames Cell Events: 5981 mean_circ: 0.905218
Ran for 397050 frames Cell Events: 5989 mean_circ: 0.905216
Ran for 397550 frames Cell Events: 5992 mean_circ: 0.905204
Ran for 398050 frames Cell Events: 5997 mean_circ: 0.905233
Ran for 398550 frames Cell Events: 6009 mean_circ: 0.905263
Ran for 399050 frames Cell Events: 6014 mean_circ: 0.905251
Ran for 400050 frames Cell Events: 6031 mean_circ: 0.905231
Ran for 400550 frames Cell Events: 6043 mean_circ: 0.905232
Ran for 401050 frames Cell Events: 6053 mean_circ: 0.90525
Ran for 401550 frames Cell Events: 6066 mean_circ: 0.905252
Ran for 402050 frames Cell Events: 6076 mean_circ: 0.905231
Ran for 403050 frames Cell Events: 6088 mean_circ: 0.905198
Ran for 403550 frames Cell Events: 6099 mean_circ: 0.905214
Ran for 404050 frames Cell Events: 6118 mean_circ: 0.905224
Ran for 404550 frames Cell Events: 6122 mean_circ: 0.905262
Ran for 405050 frames Cell Events: 6131 mean_circ: 0.905256
Ran for 405550 frames Cell Events: 6142 mean_circ: 0.905282
Ran for 407050 frames Cell Events: 6157 mean_circ: 0.905288
Ran for 407550 frames Cell Events: 6168 mean_circ: 0.905316
Ran for 408050 frames Cell Events: 6173 mean_circ: 0.90537
Ran for 408550 frames Cell Events: 6182 mean_circ: 0.905371
Ran for 409050 frames Cell Events: 6189 mean_circ: 0.905365
Ran for 409550 frames Cell Events: 6196 mean_circ: 0.905395
Ran for 410050 frames Cell Events: 6205 mean_circ: 0.905386
Ran for 411050 frames Cell Events: 6225 mean_circ: 0.905408
Ran for 411550 frames Cell Events: 6228 mean_circ: 0.905437
Ran for 412050 frames Cell Events: 6244 mean_circ: 0.905462
Ran for 413050 frames Cell Events: 6258 mean_circ: 0.905451
Ran for 413550 frames Cell Events: 6269 mean_circ: 0.9054
Ran for 414050 frames Cell Events: 6273 mean_circ: 0.905346
Ran for 414550 frames Cell Events: 6283 mean_circ: 0.905343
Ran for 415050 frames Cell Events: 6287 mean_circ: 0.905318
Ran for 416050 frames Cell Events: 6307 mean_circ: 0.90539
Ran for 416550 frames Cell Events: 6318 mean_circ: 0.90535
Ran for 417050 frames Cell Events: 6330 mean_circ: 0.905407
Ran for 418050 frames Cell Events: 6340 mean_circ: 0.905438
Ran for 418550 frames Cell Events: 6344 mean_circ: 0.905443
Ran for 419050 frames Cell Events: 6355 mean_circ: 0.905466
Ran for 419550 frames Cell Events: 6370 mean_circ: 0.905451
Ran for 420050 frames Cell Events: 6375 mean_circ: 0.905442
Ran for 421550 frames Cell Events: 6393 mean_circ: 0.905448
Ran for 422050 frames Cell Events: 6397 mean_circ: 0.905463
Ran for 423050 frames Cell Events: 6413 mean_circ: 0.905516
Ran for 423550 frames Cell Events: 6425 mean_circ: 0.905554
Ran for 424050 frames Cell Events: 6433 mean_circ: 0.905548
Ran for 424550 frames Cell Events: 6447 mean_circ: 0.905499
Ran for 425050 frames Cell Events: 6456 mean_circ: 0.905491
Ran for 426550 frames Cell Events: 6477 mean_circ: 0.905526
Ran for 428550 frames Cell Events: 6519 mean_circ: 0.905567
Ran for 429050 frames Cell Events: 6529 mean_circ: 0.905554
Ran for 430050 frames Cell Events: 6541 mean_circ: 0.905542
Ran for 430550 frames Cell Events: 6547 mean_circ: 0.905484
Ran for 431050 frames Cell Events: 6555 mean_circ: 0.90544
Ran for 431550 frames Cell Events: 6570 mean_circ: 0.905433
Ran for 432050 frames Cell Events: 6574 mean_circ: 0.905396
Ran for 432550 frames Cell Events: 6584 mean_circ: 0.905431
Ran for 433050 frames Cell Events: 6597 mean_circ: 0.905394
Ran for 434550 frames Cell Events: 6622 mean_circ: 0.905331
Ran for 435050 frames Cell Events: 6632 mean_circ: 0.905321
Ran for 435550 frames Cell Events: 6638 mean_circ: 0.905289
Ran for 436550 frames Cell Events: 6660 mean_circ: 0.905269
Ran for 437050 frames Cell Events: 6668 mean_circ: 0.905296
Ran for 437550 frames Cell Events: 6679 mean_circ: 0.905278
Ran for 438550 frames Cell Events: 6693 mean_circ: 0.905285
Ran for 439050 frames Cell Events: 6701 mean_circ: 0.905292
Ran for 439550 frames Cell Events: 6705 mean_circ: 0.905308
Ran for 440050 frames Cell Events: 6710 mean_circ: 0.905315
Ran for 440550 frames Cell Events: 6716 mean_circ: 0.905345
Ran for 441050 frames Cell Events: 6725 mean_circ: 0.905339
Ran for 442550 frames Cell Events: 6749 mean_circ: 0.905376
Ran for 443050 frames Cell Events: 6758 mean_circ: 0.905387
Ran for 443550 frames Cell Events: 6759 mean_circ: 0.905387
Ran for 444050 frames Cell Events: 6767 mean_circ: 0.90542
Ran for 444550 frames Cell Events: 6783 mean_circ: 0.905433
Ran for 445050 frames Cell Events: 6786 mean_circ: 0.90545
Ran for 445550 frames Cell Events: 6797 mean_circ: 0.905467
Ran for 446050 frames Cell Events: 6799 mean_circ: 0.90548
Ran for 446550 frames Cell Events: 6805 mean_circ: 0.905453
Ran for 447550 frames Cell Events: 6820 mean_circ: 0.905484
Ran for 448050 frames Cell Events: 6827 mean_circ: 0.905472
Ran for 448550 frames Cell Events: 6834 mean_circ: 0.905479
Ran for 449050 frames Cell Events: 6845 mean_circ: 0.905477
Ran for 450050 frames Cell Events: 6858 mean_circ: 0.905531
Ran for 450550 frames Cell Events: 6869 mean_circ: 0.905516
Ran for 451050 frames Cell Events: 6876 mean_circ: 0.9055
Ran for 451550 frames Cell Events: 6887 mean_circ: 0.905514
Ran for 452550 frames Cell Events: 6906 mean_circ: 0.905601
Ran for 453050 frames Cell Events: 6910 mean_circ: 0.905622
Ran for 454050 frames Cell Events: 6928 mean_circ: 0.905694
Ran for 455050 frames Cell Events: 6940 mean_circ: 0.905693
Ran for 456050 frames Cell Events: 6957 mean_circ: 0.905749
Ran for 456550 frames Cell Events: 6965 mean_circ: 0.905779
Ran for 458550 frames Cell Events: 7002 mean_circ: 0.905848
Ran for 459050 frames Cell Events: 7005 mean_circ: 0.905841
Ran for 459550 frames Cell Events: 7008 mean_circ: 0.905831
Ran for 460050 frames Cell Events: 7013 mean_circ: 0.905828
Ran for 460550 frames Cell Events: 7017 mean_circ: 0.905826
Ran for 461050 frames Cell Events: 7025 mean_circ: 0.905819
Ran for 461550 frames Cell Events: 7038 mean_circ: 0.905814
Ran for 462050 frames Cell Events: 7047 mean_circ: 0.905803
Ran for 462550 frames Cell Events: 7056 mean_circ: 0.905786
Ran for 463550 frames Cell Events: 7070 mean_circ: 0.905781
Ran for 464050 frames Cell Events: 7078 mean_circ: 0.905767
Ran for 464550 frames Cell Events: 7082 mean_circ: 0.905786
Ran for 465050 frames Cell Events: 7084 mean_circ: 0.905765
Ran for 465550 frames Cell Events: 7087 mean_circ: 0.905776
Ran for 466050 frames Cell Events: 7094 mean_circ: 0.905791
Ran for 466550 frames Cell Events: 7107 mean_circ: 0.905768
Ran for 467050 frames Cell Events: 7116 mean_circ: 0.905802
Ran for 467550 frames Cell Events: 7126 mean_circ: 0.905817
Ran for 468550 frames Cell Events: 7143 mean_circ: 0.905765
Ran for 469050 frames Cell Events: 7149 mean_circ: 0.905777
Ran for 469550 frames Cell Events: 7155 mean_circ: 0.905807
Ran for 470050 frames Cell Events: 7166 mean_circ: 0.905821
Ran for 470550 frames Cell Events: 7176 mean_circ: 0.905797
Ran for 471050 frames Cell Events: 7180 mean_circ: 0.905814
Ran for 472550 frames Cell Events: 7204 mean_circ: 0.905778
Ran for 473550 frames Cell Events: 7215 mean_circ: 0.905802
Ran for 474050 frames Cell Events: 7220 mean_circ: 0.905812
Ran for 474550 frames Cell Events: 7227 mean_circ: 0.905818
Ran for 475050 frames Cell Events: 7231 mean_circ: 0.905833
Ran for 475550 frames Cell Events: 7238 mean_circ: 0.905874
Ran for 476050 frames Cell Events: 7241 mean_circ: 0.905868
Ran for 476550 frames Cell Events: 7257 mean_circ: 0.905857
Ran for 477550 frames Cell Events: 7276 mean_circ: 0.905823
Ran for 478050 frames Cell Events: 7285 mean_circ: 0.905787
Ran for 478550 frames Cell Events: 7289 mean_circ: 0.905793
Ran for 479550 frames Cell Events: 7305 mean_circ: 0.905827
Ran for 480050 frames Cell Events: 7315 mean_circ: 0.905792
Ran for 480550 frames Cell Events: 7329 mean_circ: 0.905793
Ran for 481550 frames Cell Events: 7342 mean_circ: 0.905772
Ran for 482050 frames Cell Events: 7353 mean_circ: 0.905773
Ran for 482550 frames Cell Events: 7367 mean_circ: 0.905749
Ran for 483050 frames Cell Events: 7376 mean_circ: 0.905699
Ran for 483550 frames Cell Events: 7381 mean_circ: 0.905706
Ran for 484050 frames Cell Events: 7390 mean_circ: 0.905712
Ran for 485050 frames Cell Events: 7403 mean_circ: 0.905712
Ran for 486050 frames Cell Events: 7422 mean_circ: 0.905695
Ran for 486550 frames Cell Events: 7427 mean_circ: 0.905722
Ran for 487050 frames Cell Events: 7430 mean_circ: 0.905737
Ran for 487550 frames Cell Events: 7434 mean_circ: 0.905751
Ran for 488550 frames Cell Events: 7447 mean_circ: 0.905764
Ran for 489050 frames Cell Events: 7459 mean_circ: 0.905727
Ran for 489550 frames Cell Events: 7461 mean_circ: 0.905704
Ran for 490050 frames Cell Events: 7470 mean_circ: 0.905719
Ran for 490550 frames Cell Events: 7480 mean_circ: 0.905727
Ran for 491050 frames Cell Events: 7485 mean_circ: 0.905719
Ran for 492050 frames Cell Events: 7501 mean_circ: 0.905654
Ran for 492550 frames Cell Events: 7508 mean_circ: 0.905633
Ran for 494050 frames Cell Events: 7533 mean_circ: 0.905635
Ran for 496050 frames Cell Events: 7567 mean_circ: 0.905684
Ran for 496550 frames Cell Events: 7575 mean_circ: 0.905703
Ran for 497550 frames Cell Events: 7591 mean_circ: 0.90575
Ran for 498050 frames Cell Events: 7597 mean_circ: 0.905763
Ran for 498550 frames Cell Events: 7605 mean_circ: 0.905784
Ran for 499050 frames Cell Events: 7608 mean_circ: 0.905768
Ran for 499550 frames Cell Events: 7620 mean_circ: 0.905757
Ran for 500050 frames Cell Events: 7631 mean_circ: 0.905788
Ran for 500550 frames Cell Events: 7639 mean_circ: 0.905772
Ran for 501050 frames Cell Events: 7648 mean_circ: 0.905786
Ran for 501550 frames Cell Events: 7657 mean_circ: 0.905785
Ran for 502050 frames Cell Events: 7667 mean_circ: 0.905776
Ran for 502550 frames Cell Events: 7675 mean_circ: 0.90573
Ran for 503050 frames Cell Events: 7686 mean_circ: 0.905763
Ran for 503550 frames Cell Events: 7695 mean_circ: 0.905743
Ran for 504050 frames Cell Events: 7704 mean_circ: 0.905763
Ran for 504550 frames Cell Events: 7715 mean_circ: 0.905773
Ran for 505050 frames Cell Events: 7726 mean_circ: 0.905765
Ran for 505550 frames Cell Events: 7733 mean_circ: 0.90578
Ran for 506550 frames Cell Events: 7752 mean_circ: 0.905774
Ran for 507050 frames Cell Events: 7760 mean_circ: 0.905761
Ran for 507550 frames Cell Events: 7768 mean_circ: 0.905766
Ran for 508550 frames Cell Events: 7789 mean_circ: 0.905781
Ran for 509050 frames Cell Events: 7795 mean_circ: 0.905783
Ran for 509550 frames Cell Events: 7807 mean_circ: 0.905798
Ran for 510050 frames Cell Events: 7812 mean_circ: 0.905766
Ran for 511550 frames Cell Events: 7837 mean_circ: 0.905744
Ran for 512050 frames Cell Events: 7843 mean_circ: 0.905744
Ran for 512550 frames Cell Events: 7852 mean_circ: 0.905718
Ran for 513050 frames Cell Events: 7863 mean_circ: 0.905733
Ran for 514050 frames Cell Events: 7875 mean_circ: 0.905724
Ran for 515050 frames Cell Events: 7897 mean_circ: 0.905692
Ran for 515550 frames Cell Events: 7906 mean_circ: 0.905686
Ran for 516050 frames Cell Events: 7912 mean_circ: 0.905697
Ran for 516550 frames Cell Events: 7916 mean_circ: 0.905723
Ran for 517050 frames Cell Events: 7917 mean_circ: 0.905731
Ran for 517550 frames Cell Events: 7925 mean_circ: 0.905745
Ran for 518050 frames Cell Events: 7931 mean_circ: 0.905755
Ran for 518550 frames Cell Events: 7935 mean_circ: 0.905727
Ran for 519050 frames Cell Events: 7947 mean_circ: 0.905773
Ran for 519550 frames Cell Events: 7953 mean_circ: 0.905775
Ran for 520550 frames Cell Events: 7971 mean_circ: 0.905784
Ran for 521050 frames Cell Events: 7975 mean_circ: 0.905753
Ran for 521550 frames Cell Events: 7988 mean_circ: 0.905771
Ran for 522050 frames Cell Events: 7994 mean_circ: 0.90576
Ran for 522550 frames Cell Events: 7998 mean_circ: 0.905744
Ran for 523050 frames Cell Events: 8003 mean_circ: 0.905757
Ran for 523550 frames Cell Events: 8012 mean_circ: 0.905741
Ran for 524550 frames Cell Events: 8026 mean_circ: 0.905781
Ran for 525050 frames Cell Events: 8032 mean_circ: 0.905786
Ran for 525550 frames Cell Events: 8037 mean_circ: 0.905797
Ran for 526550 frames Cell Events: 8051 mean_circ: 0.905818
Ran for 528550 frames Cell Events: 8085 mean_circ: 0.905804
Ran for 529050 frames Cell Events: 8095 mean_circ: 0.905833
Ran for 529550 frames Cell Events: 8106 mean_circ: 0.905843
Ran for 530050 frames Cell Events: 8112 mean_circ: 0.905824
Ran for 530550 frames Cell Events: 8119 mean_circ: 0.905832
Ran for 531050 frames Cell Events: 8127 mean_circ: 0.905844
Ran for 531550 frames Cell Events: 8135 mean_circ: 0.905861
Ran for 532050 frames Cell Events: 8149 mean_circ: 0.905845
Ran for 532550 frames Cell Events: 8158 mean_circ: 0.905862
Ran for 533050 frames Cell Events: 8171 mean_circ: 0.905847
Ran for 533550 frames Cell Events: 8182 mean_circ: 0.905858
Ran for 534050 frames Cell Events: 8190 mean_circ: 0.905846
Ran for 534550 frames Cell Events: 8201 mean_circ: 0.905815
Ran for 535050 frames Cell Events: 8208 mean_circ: 0.905811
Ran for 535550 frames Cell Events: 8225 mean_circ: 0.905778
Ran for 536550 frames Cell Events: 8248 mean_circ: 0.905699
Ran for 537050 frames Cell Events: 8257 mean_circ: 0.905688
Ran for 537550 frames Cell Events: 8262 mean_circ: 0.905693
Ran for 538050 frames Cell Events: 8270 mean_circ: 0.905685
Ran for 538550 frames Cell Events: 8281 mean_circ: 0.90568
Ran for 539050 frames Cell Events: 8286 mean_circ: 0.905707
Ran for 539550 frames Cell Events: 8296 mean_circ: 0.905746
Ran for 540050 frames Cell Events: 8299 mean_circ: 0.905737
Ran for 541050 frames Cell Events: 8317 mean_circ: 0.90574
Ran for 542550 frames Cell Events: 8335 mean_circ: 0.90572
Ran for 543050 frames Cell Events: 8350 mean_circ: 0.905711
Ran for 544050 frames Cell Events: 8361 mean_circ: 0.905718
Ran for 544550 frames Cell Events: 8371 mean_circ: 0.905752
Ran for 545050 frames Cell Events: 8380 mean_circ: 0.905777
Ran for 545550 frames Cell Events: 8386 mean_circ: 0.905745
Ran for 546050 frames Cell Events: 8396 mean_circ: 0.905755
Ran for 547050 frames Cell Events: 8406 mean_circ: 0.905743
Ran for 548050 frames Cell Events: 8423 mean_circ: 0.905735
Ran for 548550 frames Cell Events: 8432 mean_circ: 0.905738
End this measurment..
Stopping time: 2.45609e+009
****************************************
Measurement finished
Mean Circularitiy: 0.905766
Mean Area: 619.824
Mean FL-1 intensity: 3382.41
Mean FL-2 intensity: 4369.18
Mean FL-3 intensity: 0
frames processed: 546584
Total Time: 274468ms
Number of Runs (new bg img) 0
Frames collected: 548785
Skipped frames: 24318
Rescued Frames: 2578113
Samples DAQ: 137196444
Samples written: 274392460
Samples per Channel: 137196230
FPS: 1999.45
Samples per second: 499863
Expected samples/s: 500000
Events: 8440
Events per second: 30.7504
jumped back (PMT): 21
#Frames read, frequency
# 0 frames :716043
# 1 frames :351057
# 2 frames :8565
# 3 frames :3773
# 4 frames :2206
# 5 frames :1523
# 6 frames :1926
# 7 frames :2353
# 8 frames :1798
# 9 frames :1124
# 10 frames :1029
# 11 frames :1367
# 12 frames :1286
# 13 frames :883
# 14 frames :542
# 15 frames :418
# 16 frames :300
# 17 frames :267
# 18 frames :244
# 19 frames :203
# 20 frames :148
# 21 frames :149
# 22 frames :73
# 23 frames :59
# 24 frames :43
# 25 frames :29
# 26 frames :16
# 27 frames :23
# 28 frames :12
# 29 frames :12
# 30 frames :11
# 31 frames :11
# 32 frames :6
# 33 frames :3
# 34 frames :6
# 35 frames :1
# 36 frames :6
# 37 frames :1
# 38 frames :4
# 41 frames :6
# 46 frames :1
# 53 frames :1
# 74 frames :1
M1_para.ini
[General]Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.7867
parameters.txt
#fRT-DC Configuration file# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0
Embed resource view
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Additional Information
Field | Value |
---|---|
license | Creative Commons Public Domain Dedication |
created | 3 months ago |
etag | 596eb067ce8ae1720a6b7ce0b57c3a85 |
format | RT-FDC |
id | ed3cceae-0459-420c-9597-8580b69ff9f9 |
metadata modified | 3 months ago |
mimetype | RT-DC |
package id | da28053e-9353-4ada-bf2c-6e1599c8ee2a |
position | 0 |
s3 available | True |
s3 url | https://objectstore.hpccloud.mpcdf.mpg.de/circle-8a23b5b7-6d86-46b7-9833-62373e9ec110/resource/ed3/cce/ae-0459-420c-9597-8580b69ff9f9 |
sha256 | 6c85c271ade8cb96ed504b5e5a464c4a7698d967f1a206919cf7f5d63e68f56a |
size | 145.2 MiB |
dc:experiment:date | 2017-06-12 |
dc:experiment:event count | 8,439 |
dc:experiment:run identifier | None-2af0 |
dc:experiment:run index | 1 |
dc:experiment:sample | 20170612_1833_dsRNA_U3_faster |
dc:experiment:time | 18:33:29 |
dc:fluorescence:bit depth | 16 |
dc:fluorescence:channel 1 name | FL1 |
dc:fluorescence:channel 2 name | FL2 |
dc:fluorescence:channel count | 2 |
dc:fluorescence:channels installed | 3 |
dc:fluorescence:laser 1 lambda | 488 |
dc:fluorescence:laser 1 power | 10 |
dc:fluorescence:laser 2 lambda | 561 |
dc:fluorescence:laser 2 power | 35 |
dc:fluorescence:laser 3 lambda | 640 |
dc:fluorescence:laser 3 power | 0 |
dc:fluorescence:laser count | 2 |
dc:fluorescence:lasers installed | 3 |
dc:fluorescence:sample rate | 500,000 |
dc:fluorescence:samples per event | 200 |
dc:fluorescence:signal max | 1 |
dc:fluorescence:signal min | -1 |
dc:fluorescence:trace median | 5 |
dc:imaging:flash device | LED (undefined) |
dc:imaging:flash duration | 2 |
dc:imaging:frame rate | 2,000 |
dc:imaging:pixel size | 0.34 |
dc:imaging:roi position x | 504 |
dc:imaging:roi position y | 472 |
dc:imaging:roi size x | 256 |
dc:imaging:roi size y | 96 |
dc:online contour:bin area min | 50 |
dc:online contour:bin kernel | 5 |
dc:online contour:bin threshold | 6 |
dc:online contour:image blur | 0 |
dc:online contour:no absdiff | True |
dc:setup:channel width | 20 |
dc:setup:chip region | channel |
dc:setup:flow rate | 0.06 |
dc:setup:module composition | Cell_Flow_2, Fluor |
dc:setup:software version | fRT-DC V1.06170206 | dclab 0.60.3 |
sp:cells:cell type | KC167 |
sp:cells:live | True |
sp:cells:organism | Drosophila melanogaster |
sp:chip:channel width | 20 |
sp:experiment:buffer osmolality | 325 |
sp:experiment:buffer ph | 6.8 |
sp:experiment:concentration | 5 |
sp:identifiers:labels | GFP tdTomato |
sp:identifiers:tags | NLS-GFP |