Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_16.56.47
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "ebac24699130bd9d156289ff5300d34d",
"name": "M1_20170612_1952_dsRNA_U6_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "14:57:11"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9511 (max 9512) in '9511'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.8222
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.46057e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 43 mean_circ: 0.925422
Ran for 1550 frames Cell Events: 60 mean_circ: 0.925808
Ran for 3550 frames Cell Events: 182 mean_circ: 0.924437
Ran for 4050 frames Cell Events: 211 mean_circ: 0.924682
Ran for 5050 frames Cell Events: 257 mean_circ: 0.924497
Ran for 7550 frames Cell Events: 365 mean_circ: 0.923292
Ran for 8050 frames Cell Events: 383 mean_circ: 0.924099
Ran for 8550 frames Cell Events: 410 mean_circ: 0.92471
Ran for 9050 frames Cell Events: 433 mean_circ: 0.925218
Ran for 9550 frames Cell Events: 459 mean_circ: 0.924907
Ran for 10050 frames Cell Events: 480 mean_circ: 0.925972
Ran for 12550 frames Cell Events: 622 mean_circ: 0.927133
Ran for 16550 frames Cell Events: 831 mean_circ: 0.925696
Ran for 17550 frames Cell Events: 888 mean_circ: 0.92545
Ran for 18050 frames Cell Events: 916 mean_circ: 0.92535
Ran for 18550 frames Cell Events: 944 mean_circ: 0.925184
Ran for 19550 frames Cell Events: 999 mean_circ: 0.925366
Ran for 21050 frames Cell Events: 1080 mean_circ: 0.926024
Ran for 22550 frames Cell Events: 1145 mean_circ: 0.926235
Ran for 23050 frames Cell Events: 1169 mean_circ: 0.926073
Ran for 23550 frames Cell Events: 1191 mean_circ: 0.92588
Ran for 24550 frames Cell Events: 1239 mean_circ: 0.925994
Ran for 25050 frames Cell Events: 1263 mean_circ: 0.926037
Ran for 26050 frames Cell Events: 1315 mean_circ: 0.925685
Ran for 27050 frames Cell Events: 1385 mean_circ: 0.925362
Ran for 27550 frames Cell Events: 1420 mean_circ: 0.925494
Ran for 30050 frames Cell Events: 1529 mean_circ: 0.925733
Ran for 30550 frames Cell Events: 1554 mean_circ: 0.925817
Ran for 31050 frames Cell Events: 1580 mean_circ: 0.925991
Ran for 32050 frames Cell Events: 1645 mean_circ: 0.926214
Ran for 32550 frames Cell Events: 1672 mean_circ: 0.926221
Ran for 33550 frames Cell Events: 1731 mean_circ: 0.926539
Ran for 35550 frames Cell Events: 1817 mean_circ: 0.926208
Ran for 36050 frames Cell Events: 1838 mean_circ: 0.926224
Ran for 37550 frames Cell Events: 1911 mean_circ: 0.926393
Ran for 38050 frames Cell Events: 1930 mean_circ: 0.926375
Ran for 39050 frames Cell Events: 1975 mean_circ: 0.926528
Ran for 40050 frames Cell Events: 2023 mean_circ: 0.926188
Ran for 40550 frames Cell Events: 2042 mean_circ: 0.926162
Ran for 41050 frames Cell Events: 2066 mean_circ: 0.926142
Ran for 42550 frames Cell Events: 2139 mean_circ: 0.926213
Ran for 43050 frames Cell Events: 2165 mean_circ: 0.926072
Ran for 44050 frames Cell Events: 2202 mean_circ: 0.92613
Ran for 44550 frames Cell Events: 2219 mean_circ: 0.92613
Ran for 45050 frames Cell Events: 2242 mean_circ: 0.926157
Ran for 45550 frames Cell Events: 2266 mean_circ: 0.926212
Ran for 46050 frames Cell Events: 2288 mean_circ: 0.926062
Ran for 47050 frames Cell Events: 2326 mean_circ: 0.926062
Ran for 47550 frames Cell Events: 2350 mean_circ: 0.926094
Ran for 48550 frames Cell Events: 2405 mean_circ: 0.926229
Ran for 49050 frames Cell Events: 2430 mean_circ: 0.926251
Ran for 49550 frames Cell Events: 2452 mean_circ: 0.926193
Ran for 50050 frames Cell Events: 2471 mean_circ: 0.926099
Ran for 50550 frames Cell Events: 2502 mean_circ: 0.926059
Ran for 51050 frames Cell Events: 2529 mean_circ: 0.926049
Ran for 54050 frames Cell Events: 2687 mean_circ: 0.925795
Ran for 56050 frames Cell Events: 2798 mean_circ: 0.925884
Ran for 56550 frames Cell Events: 2824 mean_circ: 0.925752
Ran for 57050 frames Cell Events: 2846 mean_circ: 0.925761
Ran for 57550 frames Cell Events: 2863 mean_circ: 0.9258
Ran for 58550 frames Cell Events: 2911 mean_circ: 0.925861
Ran for 60550 frames Cell Events: 3033 mean_circ: 0.925861
Ran for 62050 frames Cell Events: 3111 mean_circ: 0.925972
Ran for 64550 frames Cell Events: 3240 mean_circ: 0.925683
Ran for 65550 frames Cell Events: 3302 mean_circ: 0.925764
Ran for 66550 frames Cell Events: 3367 mean_circ: 0.925849
Ran for 67050 frames Cell Events: 3385 mean_circ: 0.92574
Ran for 67550 frames Cell Events: 3407 mean_circ: 0.925832
Ran for 68050 frames Cell Events: 3430 mean_circ: 0.925662
Ran for 70550 frames Cell Events: 3579 mean_circ: 0.9257
Ran for 73050 frames Cell Events: 3723 mean_circ: 0.925933
Ran for 73550 frames Cell Events: 3743 mean_circ: 0.925961
Ran for 74050 frames Cell Events: 3775 mean_circ: 0.925961
Ran for 76050 frames Cell Events: 3889 mean_circ: 0.925608
Ran for 79050 frames Cell Events: 4045 mean_circ: 0.925823
Ran for 80550 frames Cell Events: 4115 mean_circ: 0.926003
Ran for 81050 frames Cell Events: 4144 mean_circ: 0.925962
Ran for 81550 frames Cell Events: 4172 mean_circ: 0.926005
Ran for 82050 frames Cell Events: 4189 mean_circ: 0.925984
Ran for 82550 frames Cell Events: 4211 mean_circ: 0.926107
Ran for 85050 frames Cell Events: 4345 mean_circ: 0.926256
Ran for 85550 frames Cell Events: 4379 mean_circ: 0.926248
Ran for 86050 frames Cell Events: 4408 mean_circ: 0.926276
Ran for 87050 frames Cell Events: 4462 mean_circ: 0.926277
Ran for 88550 frames Cell Events: 4555 mean_circ: 0.926393
Ran for 89050 frames Cell Events: 4578 mean_circ: 0.926206
Ran for 90550 frames Cell Events: 4655 mean_circ: 0.926241
Ran for 92050 frames Cell Events: 4744 mean_circ: 0.926294
Ran for 93050 frames Cell Events: 4796 mean_circ: 0.9263
Ran for 93550 frames Cell Events: 4824 mean_circ: 0.926296
Ran for 94050 frames Cell Events: 4856 mean_circ: 0.926352
Ran for 94550 frames Cell Events: 4878 mean_circ: 0.926447
Ran for 95550 frames Cell Events: 4929 mean_circ: 0.926324
Ran for 96050 frames Cell Events: 4952 mean_circ: 0.926416
Ran for 97050 frames Cell Events: 5008 mean_circ: 0.926299
Ran for 98550 frames Cell Events: 5099 mean_circ: 0.926206
Ran for 99050 frames Cell Events: 5127 mean_circ: 0.926191
Ran for 99550 frames Cell Events: 5142 mean_circ: 0.92625
Ran for 100550 frames Cell Events: 5186 mean_circ: 0.926283
Ran for 101050 frames Cell Events: 5210 mean_circ: 0.926301
Ran for 101550 frames Cell Events: 5236 mean_circ: 0.926324
Ran for 102550 frames Cell Events: 5286 mean_circ: 0.926303
Ran for 103050 frames Cell Events: 5307 mean_circ: 0.926199
Ran for 103550 frames Cell Events: 5329 mean_circ: 0.926231
Ran for 106050 frames Cell Events: 5484 mean_circ: 0.92619
Ran for 106550 frames Cell Events: 5514 mean_circ: 0.926276
Ran for 107550 frames Cell Events: 5574 mean_circ: 0.92624
Ran for 108050 frames Cell Events: 5591 mean_circ: 0.926207
Ran for 108550 frames Cell Events: 5615 mean_circ: 0.926141
Ran for 109550 frames Cell Events: 5672 mean_circ: 0.926233
Ran for 111050 frames Cell Events: 5742 mean_circ: 0.926405
Ran for 111550 frames Cell Events: 5764 mean_circ: 0.926366
Ran for 112550 frames Cell Events: 5827 mean_circ: 0.926311
Ran for 113050 frames Cell Events: 5846 mean_circ: 0.926248
Ran for 113550 frames Cell Events: 5863 mean_circ: 0.92625
Ran for 115050 frames Cell Events: 5961 mean_circ: 0.92621
Ran for 116550 frames Cell Events: 6036 mean_circ: 0.926138
Ran for 117050 frames Cell Events: 6065 mean_circ: 0.926237
Ran for 118050 frames Cell Events: 6122 mean_circ: 0.926218
Ran for 119550 frames Cell Events: 6210 mean_circ: 0.926292
Ran for 120550 frames Cell Events: 6268 mean_circ: 0.926376
Ran for 121050 frames Cell Events: 6289 mean_circ: 0.926391
Ran for 123050 frames Cell Events: 6408 mean_circ: 0.92638
Ran for 124050 frames Cell Events: 6449 mean_circ: 0.926389
Ran for 125050 frames Cell Events: 6493 mean_circ: 0.926456
Ran for 126550 frames Cell Events: 6589 mean_circ: 0.926611
Ran for 127550 frames Cell Events: 6662 mean_circ: 0.926549
Ran for 130050 frames Cell Events: 6805 mean_circ: 0.926414
Ran for 131050 frames Cell Events: 6848 mean_circ: 0.926454
Ran for 132050 frames Cell Events: 6893 mean_circ: 0.926489
Ran for 133050 frames Cell Events: 6940 mean_circ: 0.926475
Ran for 134550 frames Cell Events: 7010 mean_circ: 0.926411
Ran for 135050 frames Cell Events: 7036 mean_circ: 0.926457
Ran for 135550 frames Cell Events: 7058 mean_circ: 0.926465
Ran for 136050 frames Cell Events: 7074 mean_circ: 0.926467
Ran for 137050 frames Cell Events: 7152 mean_circ: 0.92647
Ran for 138050 frames Cell Events: 7203 mean_circ: 0.92648
Ran for 139550 frames Cell Events: 7276 mean_circ: 0.926481
Ran for 142050 frames Cell Events: 7398 mean_circ: 0.926518
Ran for 143050 frames Cell Events: 7442 mean_circ: 0.926457
Ran for 146550 frames Cell Events: 7659 mean_circ: 0.926523
Ran for 147050 frames Cell Events: 7676 mean_circ: 0.92653
Ran for 148050 frames Cell Events: 7737 mean_circ: 0.926568
Ran for 150050 frames Cell Events: 7864 mean_circ: 0.926557
Ran for 151050 frames Cell Events: 7930 mean_circ: 0.926499
Ran for 153050 frames Cell Events: 8051 mean_circ: 0.926403
Ran for 153550 frames Cell Events: 8086 mean_circ: 0.926457
Ran for 154050 frames Cell Events: 8118 mean_circ: 0.926482
Ran for 154550 frames Cell Events: 8140 mean_circ: 0.926511
Ran for 155550 frames Cell Events: 8204 mean_circ: 0.926497
Ran for 156050 frames Cell Events: 8230 mean_circ: 0.926457
Ran for 157050 frames Cell Events: 8301 mean_circ: 0.926335
Ran for 158550 frames Cell Events: 8391 mean_circ: 0.926313
Ran for 160050 frames Cell Events: 8484 mean_circ: 0.926297
Ran for 160550 frames Cell Events: 8517 mean_circ: 0.92622
Ran for 164050 frames Cell Events: 8742 mean_circ: 0.926281
Ran for 164550 frames Cell Events: 8756 mean_circ: 0.926302
Ran for 165050 frames Cell Events: 8789 mean_circ: 0.926376
Ran for 165550 frames Cell Events: 8809 mean_circ: 0.926361
Ran for 166550 frames Cell Events: 8861 mean_circ: 0.926392
Ran for 167050 frames Cell Events: 8888 mean_circ: 0.926379
Ran for 168550 frames Cell Events: 8968 mean_circ: 0.926335
Ran for 169050 frames Cell Events: 8998 mean_circ: 0.926322
Ran for 169550 frames Cell Events: 9022 mean_circ: 0.926358
Ran for 172050 frames Cell Events: 9178 mean_circ: 0.926298
Ran for 172550 frames Cell Events: 9198 mean_circ: 0.926254
Ran for 173050 frames Cell Events: 9225 mean_circ: 0.926258
Ran for 173550 frames Cell Events: 9246 mean_circ: 0.926207
Ran for 174550 frames Cell Events: 9311 mean_circ: 0.926254
Ran for 175050 frames Cell Events: 9328 mean_circ: 0.926245
Ran for 175550 frames Cell Events: 9364 mean_circ: 0.926191
Ran for 176050 frames Cell Events: 9391 mean_circ: 0.926202
Ran for 177050 frames Cell Events: 9458 mean_circ: 0.92613
Ran for 177550 frames Cell Events: 9479 mean_circ: 0.926146
End this measurment..
Stopping time: 2.46066e+009
****************************************
Measurement finished
Mean Circularitiy: 0.926126
Mean Area: 640.737
Mean FL-1 intensity: 2974.38
Mean FL-2 intensity: 3638.68
Mean FL-3 intensity: 0
frames processed: 175216
Total Time: 89220.7ms
Number of Runs (new bg img) 0
Frames collected: 178315
Skipped frames: 31994
Rescued Frames: 3824795
Samples DAQ: 44580383
Samples written: 89157434
Samples per Channel: 44578717
FPS: 1998.58
Samples per second: 499646
Expected samples/s: 500000
Events: 9512
Events per second: 106.612
jumped back (PMT): 32
#Frames read, frequency
# 0 frames :283461
# 1 frames :58439
# 2 frames :3101
# 3 frames :1402
# 4 frames :783
# 5 frames :467
# 6 frames :1905
# 7 frames :1302
# 8 frames :1081
# 9 frames :720
# 10 frames :578
# 11 frames :445
# 12 frames :392
# 13 frames :298
# 14 frames :293
# 15 frames :272
# 16 frames :192
# 17 frames :167
# 18 frames :106
# 19 frames :139
# 20 frames :115
# 21 frames :90
# 22 frames :86
# 23 frames :67
# 24 frames :65
# 25 frames :68
# 26 frames :49
# 27 frames :52
# 28 frames :45
# 29 frames :35
# 30 frames :32
# 31 frames :40
# 32 frames :32
# 33 frames :18
# 34 frames :27
# 35 frames :20
# 36 frames :14
# 37 frames :18
# 38 frames :11
# 39 frames :16
# 40 frames :12
# 41 frames :10
# 42 frames :6
# 43 frames :13
# 44 frames :11
# 45 frames :4
# 46 frames :5
# 47 frames :9
# 48 frames :6
# 49 frames :5
# 50 frames :6
# 51 frames :2
# 52 frames :3
# 53 frames :3
# 54 frames :6
# 55 frames :4
# 56 frames :1
# 57 frames :2
# 58 frames :1
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# 60 frames :1
# 61 frames :2
# 62 frames :3
# 63 frames :2
# 64 frames :2
# 65 frames :3
# 67 frames :1
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# 92 frames :1
# 97 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.8222
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0