Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_17.17.08
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "d84e3875cc61f36b8b46f16c2f7b7e68",
"name": "M1_20170613_1606_dsRNA_V5_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:17:29"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8214 (max 8215) in '8214'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.7339
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.53338e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 1 mean_circ: 0.933346
Ran for 1050 frames Cell Events: 48 mean_circ: 0.947059
Ran for 1550 frames Cell Events: 65 mean_circ: 0.948949
Ran for 3050 frames Cell Events: 111 mean_circ: 0.94632
Ran for 3550 frames Cell Events: 120 mean_circ: 0.946716
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Ran for 5050 frames Cell Events: 156 mean_circ: 0.947529
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Ran for 7050 frames Cell Events: 225 mean_circ: 0.948518
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Ran for 63550 frames Cell Events: 1937 mean_circ: 0.947728
Ran for 64550 frames Cell Events: 1958 mean_circ: 0.947817
Ran for 65050 frames Cell Events: 1969 mean_circ: 0.947826
Ran for 65550 frames Cell Events: 1983 mean_circ: 0.947797
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Ran for 81050 frames Cell Events: 2467 mean_circ: 0.947301
Ran for 81550 frames Cell Events: 2483 mean_circ: 0.947385
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Ran for 83050 frames Cell Events: 2539 mean_circ: 0.947336
Ran for 84050 frames Cell Events: 2568 mean_circ: 0.947279
Ran for 86050 frames Cell Events: 2638 mean_circ: 0.947373
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Ran for 88050 frames Cell Events: 2716 mean_circ: 0.947362
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Ran for 91550 frames Cell Events: 2818 mean_circ: 0.947426
Ran for 93550 frames Cell Events: 2885 mean_circ: 0.947349
Ran for 94050 frames Cell Events: 2900 mean_circ: 0.947327
Ran for 94550 frames Cell Events: 2914 mean_circ: 0.947285
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Ran for 96550 frames Cell Events: 2976 mean_circ: 0.947206
Ran for 97050 frames Cell Events: 2991 mean_circ: 0.947176
Ran for 97550 frames Cell Events: 3007 mean_circ: 0.9472
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Ran for 100050 frames Cell Events: 3109 mean_circ: 0.946943
Ran for 101050 frames Cell Events: 3142 mean_circ: 0.946974
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Ran for 102050 frames Cell Events: 3185 mean_circ: 0.946915
Ran for 102550 frames Cell Events: 3206 mean_circ: 0.946922
Ran for 104050 frames Cell Events: 3253 mean_circ: 0.94693
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Ran for 121550 frames Cell Events: 3812 mean_circ: 0.947115
Ran for 124550 frames Cell Events: 3937 mean_circ: 0.947085
Ran for 125050 frames Cell Events: 3951 mean_circ: 0.947105
Ran for 127050 frames Cell Events: 4018 mean_circ: 0.947226
Ran for 128050 frames Cell Events: 4054 mean_circ: 0.947134
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Ran for 132550 frames Cell Events: 4204 mean_circ: 0.946956
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Ran for 141050 frames Cell Events: 4507 mean_circ: 0.946998
Ran for 141550 frames Cell Events: 4521 mean_circ: 0.947012
Ran for 142550 frames Cell Events: 4555 mean_circ: 0.94698
Ran for 143550 frames Cell Events: 4589 mean_circ: 0.947034
Ran for 144050 frames Cell Events: 4613 mean_circ: 0.946973
Ran for 144550 frames Cell Events: 4626 mean_circ: 0.946969
Ran for 146050 frames Cell Events: 4679 mean_circ: 0.946958
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Ran for 152050 frames Cell Events: 4883 mean_circ: 0.94694
Ran for 153050 frames Cell Events: 4909 mean_circ: 0.946941
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Ran for 172550 frames Cell Events: 5579 mean_circ: 0.946575
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Ran for 195550 frames Cell Events: 6404 mean_circ: 0.946249
Ran for 197050 frames Cell Events: 6465 mean_circ: 0.946219
Ran for 197550 frames Cell Events: 6476 mean_circ: 0.946208
Ran for 198550 frames Cell Events: 6501 mean_circ: 0.946173
Ran for 200050 frames Cell Events: 6550 mean_circ: 0.946233
Ran for 200550 frames Cell Events: 6569 mean_circ: 0.946238
Ran for 201050 frames Cell Events: 6586 mean_circ: 0.946239
Ran for 201550 frames Cell Events: 6605 mean_circ: 0.946214
Ran for 202050 frames Cell Events: 6620 mean_circ: 0.946211
Ran for 203550 frames Cell Events: 6678 mean_circ: 0.946209
Ran for 204050 frames Cell Events: 6693 mean_circ: 0.946202
Ran for 204550 frames Cell Events: 6710 mean_circ: 0.946179
Ran for 205050 frames Cell Events: 6731 mean_circ: 0.946178
Ran for 205550 frames Cell Events: 6744 mean_circ: 0.946177
Ran for 207050 frames Cell Events: 6803 mean_circ: 0.946191
Ran for 208050 frames Cell Events: 6838 mean_circ: 0.946228
Ran for 209550 frames Cell Events: 6890 mean_circ: 0.946214
Ran for 210050 frames Cell Events: 6906 mean_circ: 0.946213
Ran for 210550 frames Cell Events: 6930 mean_circ: 0.946193
Ran for 211050 frames Cell Events: 6941 mean_circ: 0.946178
Ran for 211550 frames Cell Events: 6957 mean_circ: 0.946194
Ran for 212050 frames Cell Events: 6965 mean_circ: 0.946204
Ran for 212550 frames Cell Events: 6973 mean_circ: 0.946221
Ran for 213550 frames Cell Events: 7004 mean_circ: 0.946232
Ran for 214050 frames Cell Events: 7019 mean_circ: 0.946242
Ran for 214550 frames Cell Events: 7036 mean_circ: 0.946234
Ran for 215550 frames Cell Events: 7057 mean_circ: 0.946267
Ran for 217550 frames Cell Events: 7137 mean_circ: 0.946255
Ran for 219550 frames Cell Events: 7199 mean_circ: 0.946266
Ran for 220550 frames Cell Events: 7236 mean_circ: 0.946302
Ran for 221050 frames Cell Events: 7244 mean_circ: 0.946259
Ran for 221550 frames Cell Events: 7255 mean_circ: 0.946227
Ran for 222050 frames Cell Events: 7262 mean_circ: 0.946228
Ran for 222550 frames Cell Events: 7282 mean_circ: 0.946236
Ran for 223550 frames Cell Events: 7318 mean_circ: 0.946202
Ran for 224050 frames Cell Events: 7336 mean_circ: 0.946186
Ran for 225050 frames Cell Events: 7367 mean_circ: 0.946153
Ran for 226550 frames Cell Events: 7411 mean_circ: 0.946099
Ran for 227050 frames Cell Events: 7427 mean_circ: 0.946107
Ran for 227550 frames Cell Events: 7439 mean_circ: 0.946102
Ran for 228050 frames Cell Events: 7453 mean_circ: 0.946067
Ran for 229050 frames Cell Events: 7484 mean_circ: 0.946028
Ran for 230050 frames Cell Events: 7526 mean_circ: 0.946028
Ran for 230550 frames Cell Events: 7544 mean_circ: 0.946018
Ran for 231050 frames Cell Events: 7559 mean_circ: 0.946029
Ran for 231550 frames Cell Events: 7577 mean_circ: 0.946045
Ran for 232050 frames Cell Events: 7588 mean_circ: 0.946036
Ran for 232550 frames Cell Events: 7603 mean_circ: 0.946005
Ran for 233050 frames Cell Events: 7623 mean_circ: 0.946019
Ran for 233550 frames Cell Events: 7635 mean_circ: 0.945995
Ran for 234550 frames Cell Events: 7669 mean_circ: 0.945978
Ran for 235050 frames Cell Events: 7682 mean_circ: 0.94594
Ran for 236550 frames Cell Events: 7742 mean_circ: 0.945933
Ran for 237050 frames Cell Events: 7757 mean_circ: 0.945915
Ran for 237550 frames Cell Events: 7783 mean_circ: 0.945882
Ran for 238550 frames Cell Events: 7819 mean_circ: 0.945868
Ran for 239050 frames Cell Events: 7836 mean_circ: 0.945855
Ran for 239550 frames Cell Events: 7857 mean_circ: 0.945844
Ran for 240050 frames Cell Events: 7865 mean_circ: 0.945852
Ran for 240550 frames Cell Events: 7881 mean_circ: 0.945868
Ran for 241050 frames Cell Events: 7904 mean_circ: 0.945883
Ran for 241550 frames Cell Events: 7914 mean_circ: 0.94586
Ran for 242050 frames Cell Events: 7932 mean_circ: 0.945833
Ran for 242550 frames Cell Events: 7952 mean_circ: 0.945833
Ran for 243050 frames Cell Events: 7972 mean_circ: 0.945856
Ran for 243550 frames Cell Events: 7985 mean_circ: 0.945868
Ran for 244050 frames Cell Events: 8012 mean_circ: 0.945868
Ran for 245550 frames Cell Events: 8049 mean_circ: 0.945845
Ran for 246050 frames Cell Events: 8072 mean_circ: 0.945858
Ran for 247050 frames Cell Events: 8110 mean_circ: 0.945843
Ran for 247550 frames Cell Events: 8122 mean_circ: 0.945819
Ran for 248050 frames Cell Events: 8140 mean_circ: 0.945832
Ran for 248550 frames Cell Events: 8162 mean_circ: 0.945854
Ran for 249050 frames Cell Events: 8189 mean_circ: 0.945857
Ran for 249550 frames Cell Events: 8206 mean_circ: 0.945858
End this measurment..
Stopping time: 2.53351e+009
****************************************
Measurement finished
Mean Circularitiy: 0.945854
Mean Area: 635.874
Mean FL-1 intensity: 3483.15
Mean FL-2 intensity: 3990.17
Mean FL-3 intensity: 0
frames processed: 249152
Total Time: 125024ms
Number of Runs (new bg img) 0
Frames collected: 249924
Skipped frames: 24565
Rescued Frames: 2917262
Samples DAQ: 62481058
Samples written: 124962034
Samples per Channel: 62481017
FPS: 1999.01
Samples per second: 499753
Expected samples/s: 500000
Events: 8215
Events per second: 65.7075
jumped back (PMT): 5398
#Frames read, frequency
# 0 frames :356646
# 1 frames :123905
# 2 frames :5733
# 3 frames :1981
# 4 frames :1193
# 5 frames :609
# 6 frames :1325
# 7 frames :1926
# 8 frames :1534
# 9 frames :1102
# 10 frames :692
# 11 frames :543
# 12 frames :493
# 13 frames :335
# 14 frames :283
# 15 frames :279
# 16 frames :242
# 17 frames :190
# 18 frames :168
# 19 frames :152
# 20 frames :109
# 21 frames :65
# 22 frames :57
# 23 frames :28
# 24 frames :38
# 25 frames :24
# 26 frames :30
# 27 frames :21
# 28 frames :14
# 29 frames :17
# 30 frames :13
# 31 frames :10
# 32 frames :11
# 33 frames :3
# 34 frames :8
# 35 frames :3
# 36 frames :3
# 37 frames :6
# 38 frames :8
# 39 frames :5
# 40 frames :3
# 42 frames :2
# 44 frames :2
# 45 frames :2
# 48 frames :1
# 50 frames :2
# 52 frames :1
# 57 frames :1
# 63 frames :1
# 74 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.7339
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0