Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_17.43.17
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "d6a1e7ef1a8593a29983c35ec63d89b3",
"name": "M1_20170615_1453_dsRNA_Y4_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:43:39"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8729 (max 8730) in '8729'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6781
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.28545e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 11 mean_circ: 0.920312
Ran for 1550 frames Cell Events: 77 mean_circ: 0.917058
Ran for 2050 frames Cell Events: 89 mean_circ: 0.921078
Ran for 3050 frames Cell Events: 120 mean_circ: 0.923671
Ran for 3550 frames Cell Events: 141 mean_circ: 0.921246
Ran for 4050 frames Cell Events: 156 mean_circ: 0.920792
Ran for 4550 frames Cell Events: 172 mean_circ: 0.921752
Ran for 5050 frames Cell Events: 188 mean_circ: 0.919808
Ran for 5550 frames Cell Events: 199 mean_circ: 0.920883
Ran for 6550 frames Cell Events: 235 mean_circ: 0.922095
Ran for 7050 frames Cell Events: 249 mean_circ: 0.923133
Ran for 7550 frames Cell Events: 261 mean_circ: 0.923145
Ran for 8550 frames Cell Events: 274 mean_circ: 0.923564
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Ran for 10050 frames Cell Events: 315 mean_circ: 0.923222
Ran for 11050 frames Cell Events: 367 mean_circ: 0.924873
Ran for 11550 frames Cell Events: 386 mean_circ: 0.924545
Ran for 12050 frames Cell Events: 407 mean_circ: 0.924627
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Ran for 13550 frames Cell Events: 450 mean_circ: 0.925621
Ran for 15050 frames Cell Events: 497 mean_circ: 0.925001
Ran for 17050 frames Cell Events: 568 mean_circ: 0.925805
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Ran for 74050 frames Cell Events: 2540 mean_circ: 0.926988
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Ran for 77550 frames Cell Events: 2657 mean_circ: 0.926704
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Ran for 78550 frames Cell Events: 2694 mean_circ: 0.92647
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Ran for 85550 frames Cell Events: 2960 mean_circ: 0.926272
Ran for 86550 frames Cell Events: 2994 mean_circ: 0.926341
Ran for 87050 frames Cell Events: 3005 mean_circ: 0.926336
Ran for 87550 frames Cell Events: 3024 mean_circ: 0.926378
Ran for 88550 frames Cell Events: 3055 mean_circ: 0.926309
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Ran for 90550 frames Cell Events: 3116 mean_circ: 0.926133
Ran for 91550 frames Cell Events: 3152 mean_circ: 0.926037
Ran for 92050 frames Cell Events: 3177 mean_circ: 0.92606
Ran for 92550 frames Cell Events: 3194 mean_circ: 0.926057
Ran for 93050 frames Cell Events: 3213 mean_circ: 0.925905
Ran for 93550 frames Cell Events: 3236 mean_circ: 0.925912
Ran for 94050 frames Cell Events: 3253 mean_circ: 0.925844
Ran for 94550 frames Cell Events: 3272 mean_circ: 0.925863
Ran for 95050 frames Cell Events: 3296 mean_circ: 0.925887
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Ran for 96550 frames Cell Events: 3367 mean_circ: 0.925806
Ran for 97050 frames Cell Events: 3378 mean_circ: 0.925786
Ran for 97550 frames Cell Events: 3394 mean_circ: 0.925795
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Ran for 100550 frames Cell Events: 3487 mean_circ: 0.925963
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Ran for 103550 frames Cell Events: 3594 mean_circ: 0.926157
Ran for 104550 frames Cell Events: 3632 mean_circ: 0.926117
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Ran for 106050 frames Cell Events: 3672 mean_circ: 0.926089
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Ran for 122050 frames Cell Events: 4217 mean_circ: 0.925839
Ran for 122550 frames Cell Events: 4233 mean_circ: 0.925749
Ran for 123550 frames Cell Events: 4279 mean_circ: 0.92569
Ran for 124050 frames Cell Events: 4290 mean_circ: 0.925686
Ran for 126050 frames Cell Events: 4352 mean_circ: 0.92572
Ran for 127550 frames Cell Events: 4415 mean_circ: 0.9256
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Ran for 131050 frames Cell Events: 4512 mean_circ: 0.925489
Ran for 131550 frames Cell Events: 4526 mean_circ: 0.925528
Ran for 132050 frames Cell Events: 4549 mean_circ: 0.925526
Ran for 132550 frames Cell Events: 4570 mean_circ: 0.925537
Ran for 133050 frames Cell Events: 4589 mean_circ: 0.925572
Ran for 133550 frames Cell Events: 4602 mean_circ: 0.92555
Ran for 134050 frames Cell Events: 4616 mean_circ: 0.925512
Ran for 134550 frames Cell Events: 4638 mean_circ: 0.925486
Ran for 135050 frames Cell Events: 4648 mean_circ: 0.925512
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Ran for 138050 frames Cell Events: 4748 mean_circ: 0.925588
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Ran for 141550 frames Cell Events: 4869 mean_circ: 0.925611
Ran for 142050 frames Cell Events: 4892 mean_circ: 0.925653
Ran for 142550 frames Cell Events: 4913 mean_circ: 0.925639
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Ran for 143550 frames Cell Events: 4950 mean_circ: 0.925663
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Ran for 147050 frames Cell Events: 5085 mean_circ: 0.925682
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Ran for 172050 frames Cell Events: 5892 mean_circ: 0.9258
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Ran for 182050 frames Cell Events: 6217 mean_circ: 0.925556
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Ran for 192550 frames Cell Events: 6545 mean_circ: 0.925435
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Ran for 193550 frames Cell Events: 6586 mean_circ: 0.925414
Ran for 194050 frames Cell Events: 6595 mean_circ: 0.925404
Ran for 194550 frames Cell Events: 6609 mean_circ: 0.925402
Ran for 195050 frames Cell Events: 6619 mean_circ: 0.925378
Ran for 196550 frames Cell Events: 6676 mean_circ: 0.92535
Ran for 197550 frames Cell Events: 6713 mean_circ: 0.925329
Ran for 198050 frames Cell Events: 6731 mean_circ: 0.925346
Ran for 198550 frames Cell Events: 6744 mean_circ: 0.92532
Ran for 199050 frames Cell Events: 6757 mean_circ: 0.925311
Ran for 199550 frames Cell Events: 6772 mean_circ: 0.925294
Ran for 200050 frames Cell Events: 6794 mean_circ: 0.925294
Ran for 200550 frames Cell Events: 6814 mean_circ: 0.925314
Ran for 201050 frames Cell Events: 6827 mean_circ: 0.925323
Ran for 201550 frames Cell Events: 6841 mean_circ: 0.925285
Ran for 202050 frames Cell Events: 6866 mean_circ: 0.925302
Ran for 202550 frames Cell Events: 6881 mean_circ: 0.925235
Ran for 203050 frames Cell Events: 6908 mean_circ: 0.925214
Ran for 205050 frames Cell Events: 6973 mean_circ: 0.9253
Ran for 205550 frames Cell Events: 6990 mean_circ: 0.925283
Ran for 206050 frames Cell Events: 6999 mean_circ: 0.92527
Ran for 207050 frames Cell Events: 7033 mean_circ: 0.92527
Ran for 207550 frames Cell Events: 7044 mean_circ: 0.925282
Ran for 208050 frames Cell Events: 7056 mean_circ: 0.925284
Ran for 208550 frames Cell Events: 7070 mean_circ: 0.925289
Ran for 210050 frames Cell Events: 7105 mean_circ: 0.925316
Ran for 210550 frames Cell Events: 7119 mean_circ: 0.925317
Ran for 211550 frames Cell Events: 7155 mean_circ: 0.925335
Ran for 212050 frames Cell Events: 7173 mean_circ: 0.925307
Ran for 212550 frames Cell Events: 7190 mean_circ: 0.925307
Ran for 213050 frames Cell Events: 7212 mean_circ: 0.925319
Ran for 213550 frames Cell Events: 7232 mean_circ: 0.925352
Ran for 214050 frames Cell Events: 7241 mean_circ: 0.925349
Ran for 215550 frames Cell Events: 7285 mean_circ: 0.925353
Ran for 216050 frames Cell Events: 7298 mean_circ: 0.925346
Ran for 216550 frames Cell Events: 7312 mean_circ: 0.92535
Ran for 217050 frames Cell Events: 7320 mean_circ: 0.925354
Ran for 218050 frames Cell Events: 7353 mean_circ: 0.92538
Ran for 218550 frames Cell Events: 7369 mean_circ: 0.92539
Ran for 219550 frames Cell Events: 7403 mean_circ: 0.925395
Ran for 220050 frames Cell Events: 7423 mean_circ: 0.92536
Ran for 220550 frames Cell Events: 7440 mean_circ: 0.925342
Ran for 221050 frames Cell Events: 7455 mean_circ: 0.925363
Ran for 221550 frames Cell Events: 7470 mean_circ: 0.925306
Ran for 222050 frames Cell Events: 7485 mean_circ: 0.925284
Ran for 223050 frames Cell Events: 7514 mean_circ: 0.925277
Ran for 223550 frames Cell Events: 7524 mean_circ: 0.925268
Ran for 224050 frames Cell Events: 7534 mean_circ: 0.925259
Ran for 224550 frames Cell Events: 7556 mean_circ: 0.925269
Ran for 225050 frames Cell Events: 7576 mean_circ: 0.92521
Ran for 225550 frames Cell Events: 7586 mean_circ: 0.925188
Ran for 226050 frames Cell Events: 7605 mean_circ: 0.925145
Ran for 226550 frames Cell Events: 7619 mean_circ: 0.925155
Ran for 227050 frames Cell Events: 7637 mean_circ: 0.925166
Ran for 227550 frames Cell Events: 7651 mean_circ: 0.925189
Ran for 228050 frames Cell Events: 7665 mean_circ: 0.925172
Ran for 229050 frames Cell Events: 7691 mean_circ: 0.925184
Ran for 229550 frames Cell Events: 7706 mean_circ: 0.925195
Ran for 230050 frames Cell Events: 7724 mean_circ: 0.925218
Ran for 230550 frames Cell Events: 7745 mean_circ: 0.925186
Ran for 231050 frames Cell Events: 7770 mean_circ: 0.925228
Ran for 232050 frames Cell Events: 7811 mean_circ: 0.925257
Ran for 232550 frames Cell Events: 7825 mean_circ: 0.925261
Ran for 233050 frames Cell Events: 7848 mean_circ: 0.925297
Ran for 233550 frames Cell Events: 7871 mean_circ: 0.925266
Ran for 234550 frames Cell Events: 7895 mean_circ: 0.925234
Ran for 236050 frames Cell Events: 7944 mean_circ: 0.925259
Ran for 237550 frames Cell Events: 7989 mean_circ: 0.925252
Ran for 238050 frames Cell Events: 8003 mean_circ: 0.925255
Ran for 238550 frames Cell Events: 8023 mean_circ: 0.925297
Ran for 239050 frames Cell Events: 8047 mean_circ: 0.925318
Ran for 240050 frames Cell Events: 8065 mean_circ: 0.92531
Ran for 240550 frames Cell Events: 8088 mean_circ: 0.925292
Ran for 241050 frames Cell Events: 8104 mean_circ: 0.925318
Ran for 242050 frames Cell Events: 8147 mean_circ: 0.92532
Ran for 243050 frames Cell Events: 8182 mean_circ: 0.925349
Ran for 244550 frames Cell Events: 8238 mean_circ: 0.925301
Ran for 245550 frames Cell Events: 8271 mean_circ: 0.925309
Ran for 246050 frames Cell Events: 8297 mean_circ: 0.925306
Ran for 246550 frames Cell Events: 8314 mean_circ: 0.925278
Ran for 247550 frames Cell Events: 8359 mean_circ: 0.925251
Ran for 248050 frames Cell Events: 8379 mean_circ: 0.925265
Ran for 248550 frames Cell Events: 8397 mean_circ: 0.925249
Ran for 249550 frames Cell Events: 8427 mean_circ: 0.925201
Ran for 251050 frames Cell Events: 8467 mean_circ: 0.925204
Ran for 251550 frames Cell Events: 8483 mean_circ: 0.925213
Ran for 253050 frames Cell Events: 8537 mean_circ: 0.925178
Ran for 253550 frames Cell Events: 8547 mean_circ: 0.92517
Ran for 254050 frames Cell Events: 8565 mean_circ: 0.925196
Ran for 255050 frames Cell Events: 8600 mean_circ: 0.925204
Ran for 255550 frames Cell Events: 8614 mean_circ: 0.925181
Ran for 256550 frames Cell Events: 8652 mean_circ: 0.925191
Ran for 257050 frames Cell Events: 8671 mean_circ: 0.92518
Ran for 257550 frames Cell Events: 8690 mean_circ: 0.925183
Ran for 258550 frames Cell Events: 8725 mean_circ: 0.925179
End this measurment..
Stopping time: 1.28674e+008
****************************************
Measurement finished
Mean Circularitiy: 0.925175
Mean Area: 725.709
Mean FL-1 intensity: 2948.09
Mean FL-2 intensity: 3431.55
Mean FL-3 intensity: 0
frames processed: 258160
Total Time: 129392ms
Number of Runs (new bg img) 0
Frames collected: 258693
Skipped frames: 8815
Rescued Frames: 715453
Samples DAQ: 64673167
Samples written: 129346096
Samples per Channel: 64673048
FPS: 1999.3
Samples per second: 499823
Expected samples/s: 500000
Events: 8730
Events per second: 67.4694
jumped back (PMT): 16
#Frames read, frequency
# 0 frames :364000
# 1 frames :134035
# 2 frames :5517
# 3 frames :2025
# 4 frames :1220
# 5 frames :516
# 6 frames :1608
# 7 frames :2288
# 8 frames :1160
# 9 frames :774
# 10 frames :778
# 11 frames :1002
# 12 frames :574
# 13 frames :423
# 14 frames :268
# 15 frames :221
# 16 frames :217
# 17 frames :185
# 18 frames :108
# 19 frames :83
# 20 frames :75
# 21 frames :48
# 22 frames :42
# 23 frames :35
# 24 frames :32
# 25 frames :30
# 26 frames :15
# 27 frames :17
# 28 frames :13
# 29 frames :6
# 30 frames :11
# 31 frames :8
# 32 frames :11
# 33 frames :2
# 34 frames :4
# 35 frames :2
# 36 frames :1
# 37 frames :3
# 38 frames :1
# 39 frames :4
# 40 frames :2
# 41 frames :4
# 42 frames :1
# 44 frames :1
# 45 frames :1
# 47 frames :1
# 48 frames :2
# 49 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6781
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0