Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_17.45.21
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "eee63ec820e202289d541e394f6b157f",
"name": "M1_20170615_1533_dsRNA_Z1_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:45:45"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 10000 (max 10001) in
'10000'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6851
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.30977e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 17 mean_circ: 0.907673
Ran for 2550 frames Cell Events: 156 mean_circ: 0.932424
Ran for 3050 frames Cell Events: 179 mean_circ: 0.934711
Ran for 7050 frames Cell Events: 410 mean_circ: 0.934534
Ran for 8050 frames Cell Events: 478 mean_circ: 0.933251
Ran for 8550 frames Cell Events: 529 mean_circ: 0.933251
Ran for 9550 frames Cell Events: 611 mean_circ: 0.934534
Ran for 10050 frames Cell Events: 652 mean_circ: 0.934261
Ran for 14550 frames Cell Events: 1042 mean_circ: 0.932867
Ran for 16550 frames Cell Events: 1197 mean_circ: 0.933368
Ran for 17050 frames Cell Events: 1229 mean_circ: 0.933244
Ran for 17550 frames Cell Events: 1269 mean_circ: 0.933338
Ran for 20050 frames Cell Events: 1483 mean_circ: 0.933247
Ran for 22050 frames Cell Events: 1643 mean_circ: 0.933753
Ran for 23550 frames Cell Events: 1767 mean_circ: 0.933852
Ran for 24050 frames Cell Events: 1806 mean_circ: 0.933634
Ran for 26050 frames Cell Events: 1963 mean_circ: 0.934132
Ran for 28050 frames Cell Events: 2118 mean_circ: 0.93366
Ran for 28550 frames Cell Events: 2157 mean_circ: 0.933597
Ran for 32050 frames Cell Events: 2443 mean_circ: 0.933148
Ran for 33550 frames Cell Events: 2561 mean_circ: 0.933502
Ran for 35050 frames Cell Events: 2664 mean_circ: 0.933663
Ran for 36550 frames Cell Events: 2785 mean_circ: 0.933285
Ran for 40050 frames Cell Events: 3113 mean_circ: 0.933015
Ran for 40550 frames Cell Events: 3140 mean_circ: 0.933159
Ran for 46550 frames Cell Events: 3650 mean_circ: 0.932812
Ran for 47050 frames Cell Events: 3686 mean_circ: 0.932837
Ran for 48550 frames Cell Events: 3805 mean_circ: 0.932856
Ran for 49550 frames Cell Events: 3857 mean_circ: 0.932711
Ran for 51050 frames Cell Events: 3982 mean_circ: 0.932711
Ran for 52050 frames Cell Events: 4071 mean_circ: 0.932703
Ran for 55050 frames Cell Events: 4298 mean_circ: 0.932735
Ran for 56050 frames Cell Events: 4372 mean_circ: 0.932634
Ran for 57050 frames Cell Events: 4443 mean_circ: 0.932621
Ran for 58050 frames Cell Events: 4516 mean_circ: 0.932625
Ran for 59550 frames Cell Events: 4652 mean_circ: 0.932406
Ran for 60050 frames Cell Events: 4688 mean_circ: 0.932405
Ran for 60550 frames Cell Events: 4725 mean_circ: 0.932359
Ran for 61050 frames Cell Events: 4756 mean_circ: 0.932343
Ran for 61550 frames Cell Events: 4786 mean_circ: 0.932369
Ran for 62050 frames Cell Events: 4815 mean_circ: 0.932395
Ran for 62550 frames Cell Events: 4849 mean_circ: 0.932459
Ran for 63550 frames Cell Events: 4935 mean_circ: 0.932433
Ran for 64550 frames Cell Events: 5003 mean_circ: 0.932411
Ran for 65550 frames Cell Events: 5074 mean_circ: 0.932398
Ran for 70550 frames Cell Events: 5479 mean_circ: 0.93236
Ran for 71050 frames Cell Events: 5525 mean_circ: 0.932355
Ran for 71550 frames Cell Events: 5556 mean_circ: 0.93234
Ran for 75050 frames Cell Events: 5801 mean_circ: 0.932176
Ran for 78050 frames Cell Events: 6010 mean_circ: 0.932198
Ran for 80050 frames Cell Events: 6168 mean_circ: 0.932346
Ran for 80550 frames Cell Events: 6203 mean_circ: 0.932346
Ran for 81550 frames Cell Events: 6294 mean_circ: 0.932303
Ran for 86550 frames Cell Events: 6703 mean_circ: 0.932185
Ran for 87050 frames Cell Events: 6733 mean_circ: 0.932152
Ran for 94050 frames Cell Events: 7365 mean_circ: 0.932145
Ran for 94550 frames Cell Events: 7400 mean_circ: 0.932246
Ran for 95050 frames Cell Events: 7454 mean_circ: 0.932259
Ran for 96050 frames Cell Events: 7555 mean_circ: 0.932197
Ran for 97550 frames Cell Events: 7655 mean_circ: 0.932278
Ran for 98550 frames Cell Events: 7738 mean_circ: 0.932319
Ran for 100050 frames Cell Events: 7864 mean_circ: 0.932255
Ran for 100550 frames Cell Events: 7913 mean_circ: 0.932244
Ran for 101050 frames Cell Events: 7948 mean_circ: 0.932254
Ran for 102550 frames Cell Events: 8063 mean_circ: 0.9323
Ran for 103050 frames Cell Events: 8108 mean_circ: 0.932387
Ran for 104550 frames Cell Events: 8234 mean_circ: 0.932372
Ran for 106050 frames Cell Events: 8364 mean_circ: 0.93228
Ran for 107550 frames Cell Events: 8463 mean_circ: 0.932207
Ran for 110050 frames Cell Events: 8684 mean_circ: 0.932246
Ran for 110550 frames Cell Events: 8713 mean_circ: 0.932275
Ran for 112550 frames Cell Events: 8881 mean_circ: 0.93229
Ran for 114550 frames Cell Events: 9033 mean_circ: 0.932185
Ran for 116050 frames Cell Events: 9163 mean_circ: 0.93222
Ran for 117050 frames Cell Events: 9240 mean_circ: 0.932117
Ran for 117550 frames Cell Events: 9271 mean_circ: 0.932117
Ran for 119550 frames Cell Events: 9424 mean_circ: 0.932161
Ran for 120050 frames Cell Events: 9466 mean_circ: 0.932183
Ran for 123050 frames Cell Events: 9700 mean_circ: 0.932061
Ran for 124550 frames Cell Events: 9795 mean_circ: 0.932014
Ran for 125050 frames Cell Events: 9828 mean_circ: 0.93204
Ran for 125550 frames Cell Events: 9860 mean_circ: 0.932001
End this measurment.
because stop condition (time/nr of cells) was met
Stopping time: 1.31041e+008
****************************************
Measurement finished
Mean Circularitiy: 0.931936
Mean Area: 629.275
Mean FL-1 intensity: 3111.02
Mean FL-2 intensity: 3232.63
Mean FL-3 intensity: 0
frames processed: 123992
Total Time: 63989.3ms
Number of Runs (new bg img) 0
Frames collected: 127794
Skipped frames: 18488
Rescued Frames: 1087022
Samples DAQ: 31950864
Samples written: 63896782
Samples per Channel: 31948391
FPS: 1997.11
Samples per second: 499277
Expected samples/s: 500000
Events: 10001
Events per second: 156.292
jumped back (PMT): 26
#Frames read, frequency
# 0 frames :205822
# 1 frames :29279
# 2 frames :1875
# 3 frames :909
# 4 frames :415
# 5 frames :245
# 6 frames :2061
# 7 frames :829
# 8 frames :816
# 9 frames :535
# 10 frames :397
# 11 frames :308
# 12 frames :231
# 13 frames :208
# 14 frames :270
# 15 frames :233
# 16 frames :199
# 17 frames :140
# 18 frames :98
# 19 frames :102
# 20 frames :85
# 21 frames :97
# 22 frames :90
# 23 frames :76
# 24 frames :57
# 25 frames :59
# 26 frames :49
# 27 frames :46
# 28 frames :46
# 29 frames :37
# 30 frames :25
# 31 frames :30
# 32 frames :22
# 33 frames :18
# 34 frames :31
# 35 frames :19
# 36 frames :18
# 37 frames :10
# 38 frames :14
# 39 frames :18
# 40 frames :14
# 41 frames :11
# 42 frames :4
# 43 frames :16
# 44 frames :12
# 45 frames :7
# 46 frames :13
# 47 frames :8
# 48 frames :2
# 50 frames :11
# 51 frames :4
# 52 frames :6
# 53 frames :3
# 55 frames :4
# 56 frames :1
# 57 frames :3
# 58 frames :3
# 59 frames :5
# 60 frames :4
# 61 frames :2
# 62 frames :1
# 63 frames :1
# 65 frames :2
# 69 frames :2
# 70 frames :1
# 73 frames :1
# 81 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6851
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0