Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_17.55.01
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "6ea7dd6625aa43d291178249cf63d3bb",
"name": "M1_20170615_1817_dsRNA_W4_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:55:25"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9570 (max 9571) in '9570'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6608
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.40829e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 18 mean_circ: 0.944161
Ran for 1050 frames Cell Events: 45 mean_circ: 0.952011
Ran for 1550 frames Cell Events: 62 mean_circ: 0.950543
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Ran for 3550 frames Cell Events: 125 mean_circ: 0.94603
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Ran for 74050 frames Cell Events: 3287 mean_circ: 0.943621
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Ran for 82550 frames Cell Events: 3644 mean_circ: 0.943963
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Ran for 92050 frames Cell Events: 4080 mean_circ: 0.943955
Ran for 92550 frames Cell Events: 4093 mean_circ: 0.943968
Ran for 93050 frames Cell Events: 4113 mean_circ: 0.943987
Ran for 93550 frames Cell Events: 4149 mean_circ: 0.943956
Ran for 94050 frames Cell Events: 4178 mean_circ: 0.943899
Ran for 97050 frames Cell Events: 4309 mean_circ: 0.944009
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Ran for 100050 frames Cell Events: 4461 mean_circ: 0.944148
Ran for 101050 frames Cell Events: 4510 mean_circ: 0.944199
Ran for 101550 frames Cell Events: 4530 mean_circ: 0.944188
Ran for 102050 frames Cell Events: 4557 mean_circ: 0.944191
Ran for 102550 frames Cell Events: 4578 mean_circ: 0.944216
Ran for 103050 frames Cell Events: 4614 mean_circ: 0.944106
Ran for 103550 frames Cell Events: 4639 mean_circ: 0.944112
Ran for 104550 frames Cell Events: 4687 mean_circ: 0.944078
Ran for 105050 frames Cell Events: 4701 mean_circ: 0.94398
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Ran for 106050 frames Cell Events: 4762 mean_circ: 0.943934
Ran for 107050 frames Cell Events: 4816 mean_circ: 0.944012
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Ran for 121550 frames Cell Events: 5466 mean_circ: 0.943757
Ran for 122050 frames Cell Events: 5487 mean_circ: 0.94373
Ran for 123550 frames Cell Events: 5547 mean_circ: 0.943746
Ran for 126050 frames Cell Events: 5674 mean_circ: 0.943794
Ran for 126550 frames Cell Events: 5696 mean_circ: 0.943826
Ran for 127050 frames Cell Events: 5710 mean_circ: 0.943865
Ran for 127550 frames Cell Events: 5737 mean_circ: 0.943879
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Ran for 132050 frames Cell Events: 5927 mean_circ: 0.943798
Ran for 132550 frames Cell Events: 5956 mean_circ: 0.943798
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Ran for 141050 frames Cell Events: 6305 mean_circ: 0.94385
Ran for 141550 frames Cell Events: 6323 mean_circ: 0.943849
Ran for 142550 frames Cell Events: 6364 mean_circ: 0.943906
Ran for 143050 frames Cell Events: 6379 mean_circ: 0.943903
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Ran for 144050 frames Cell Events: 6427 mean_circ: 0.943916
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Ran for 146050 frames Cell Events: 6513 mean_circ: 0.943925
Ran for 146550 frames Cell Events: 6530 mean_circ: 0.943894
Ran for 147050 frames Cell Events: 6545 mean_circ: 0.943902
Ran for 147550 frames Cell Events: 6571 mean_circ: 0.943881
Ran for 148050 frames Cell Events: 6598 mean_circ: 0.943874
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Ran for 172550 frames Cell Events: 7665 mean_circ: 0.943759
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Ran for 190050 frames Cell Events: 8457 mean_circ: 0.943791
Ran for 190550 frames Cell Events: 8476 mean_circ: 0.943772
Ran for 191550 frames Cell Events: 8512 mean_circ: 0.943755
Ran for 192050 frames Cell Events: 8540 mean_circ: 0.94375
Ran for 192550 frames Cell Events: 8559 mean_circ: 0.943766
Ran for 193050 frames Cell Events: 8581 mean_circ: 0.943765
Ran for 193550 frames Cell Events: 8612 mean_circ: 0.943767
Ran for 194050 frames Cell Events: 8637 mean_circ: 0.943773
Ran for 195550 frames Cell Events: 8729 mean_circ: 0.943782
Ran for 196050 frames Cell Events: 8751 mean_circ: 0.943782
Ran for 196550 frames Cell Events: 8767 mean_circ: 0.94376
Ran for 197050 frames Cell Events: 8791 mean_circ: 0.943758
Ran for 197550 frames Cell Events: 8805 mean_circ: 0.943753
Ran for 198050 frames Cell Events: 8820 mean_circ: 0.943744
Ran for 198550 frames Cell Events: 8851 mean_circ: 0.943739
Ran for 199550 frames Cell Events: 8903 mean_circ: 0.94375
Ran for 200050 frames Cell Events: 8929 mean_circ: 0.943756
Ran for 200550 frames Cell Events: 8961 mean_circ: 0.943749
Ran for 201550 frames Cell Events: 9024 mean_circ: 0.943709
Ran for 202050 frames Cell Events: 9042 mean_circ: 0.943719
Ran for 202550 frames Cell Events: 9062 mean_circ: 0.943727
Ran for 203550 frames Cell Events: 9108 mean_circ: 0.94371
Ran for 204050 frames Cell Events: 9127 mean_circ: 0.943725
Ran for 204550 frames Cell Events: 9151 mean_circ: 0.943729
Ran for 205050 frames Cell Events: 9174 mean_circ: 0.943753
Ran for 205550 frames Cell Events: 9195 mean_circ: 0.943731
Ran for 208550 frames Cell Events: 9354 mean_circ: 0.943678
Ran for 209050 frames Cell Events: 9383 mean_circ: 0.943676
Ran for 210550 frames Cell Events: 9450 mean_circ: 0.943687
Ran for 211050 frames Cell Events: 9459 mean_circ: 0.943681
Ran for 211550 frames Cell Events: 9483 mean_circ: 0.943679
Ran for 212050 frames Cell Events: 9505 mean_circ: 0.943686
Ran for 213050 frames Cell Events: 9543 mean_circ: 0.943715
Ran for 213550 frames Cell Events: 9562 mean_circ: 0.943686
End this measurment..
Stopping time: 1.40936e+008
****************************************
Measurement finished
Mean Circularitiy: 0.943694
Mean Area: 596.451
Mean FL-1 intensity: 3070.76
Mean FL-2 intensity: 3939.53
Mean FL-3 intensity: 0
frames processed: 213135
Total Time: 106957ms
Number of Runs (new bg img) 0
Frames collected: 213715
Skipped frames: 15145
Rescued Frames: 1517386
Samples DAQ: 53428767
Samples written: 106857268
Samples per Channel: 53428634
FPS: 1998.13
Samples per second: 499532
Expected samples/s: 500000
Events: 9571
Events per second: 89.4842
jumped back (PMT): 17
#Frames read, frequency
# 0 frames :299389
# 1 frames :110145
# 2 frames :4610
# 3 frames :1894
# 4 frames :667
# 5 frames :3426
# 6 frames :1878
# 7 frames :1109
# 8 frames :668
# 9 frames :432
# 10 frames :613
# 11 frames :1016
# 12 frames :373
# 13 frames :254
# 14 frames :183
# 15 frames :125
# 16 frames :134
# 17 frames :117
# 18 frames :78
# 19 frames :65
# 20 frames :39
# 21 frames :33
# 22 frames :21
# 23 frames :16
# 24 frames :12
# 25 frames :13
# 26 frames :6
# 27 frames :5
# 28 frames :1
# 29 frames :4
# 31 frames :2
# 32 frames :2
# 33 frames :1
# 34 frames :2
# 35 frames :2
# 39 frames :1
# 47 frames :1
# 61 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6608
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0