Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_17.56.02
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "314cc66e8c25fc674b83e6ead8089e6a",
"name": "M1_20170615_1829_dsRNA_X6_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:56:23"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8268 (max 8269) in '8268'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6288
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.41547e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 1 mean_circ: 0.953339
Ran for 550 frames Cell Events: 23 mean_circ: 0.9423
Ran for 1050 frames Cell Events: 46 mean_circ: 0.942811
Ran for 1550 frames Cell Events: 66 mean_circ: 0.945468
Ran for 2050 frames Cell Events: 83 mean_circ: 0.945868
Ran for 2550 frames Cell Events: 100 mean_circ: 0.945961
Ran for 3050 frames Cell Events: 115 mean_circ: 0.945752
Ran for 3550 frames Cell Events: 126 mean_circ: 0.945826
Ran for 4050 frames Cell Events: 141 mean_circ: 0.946526
Ran for 6050 frames Cell Events: 226 mean_circ: 0.944841
Ran for 7550 frames Cell Events: 300 mean_circ: 0.943472
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Ran for 13050 frames Cell Events: 523 mean_circ: 0.943955
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Ran for 85550 frames Cell Events: 3267 mean_circ: 0.941467
Ran for 86550 frames Cell Events: 3293 mean_circ: 0.941491
Ran for 87050 frames Cell Events: 3313 mean_circ: 0.94143
Ran for 87550 frames Cell Events: 3324 mean_circ: 0.941439
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Ran for 91050 frames Cell Events: 3478 mean_circ: 0.941073
Ran for 91550 frames Cell Events: 3493 mean_circ: 0.941097
Ran for 93050 frames Cell Events: 3557 mean_circ: 0.941076
Ran for 94050 frames Cell Events: 3589 mean_circ: 0.941089
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Ran for 96050 frames Cell Events: 3662 mean_circ: 0.941064
Ran for 96550 frames Cell Events: 3684 mean_circ: 0.94105
Ran for 97050 frames Cell Events: 3697 mean_circ: 0.941053
Ran for 97550 frames Cell Events: 3716 mean_circ: 0.941088
Ran for 98050 frames Cell Events: 3737 mean_circ: 0.941074
Ran for 98550 frames Cell Events: 3750 mean_circ: 0.94112
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Ran for 99550 frames Cell Events: 3795 mean_circ: 0.941063
Ran for 100050 frames Cell Events: 3816 mean_circ: 0.941041
Ran for 101550 frames Cell Events: 3873 mean_circ: 0.940998
Ran for 102050 frames Cell Events: 3888 mean_circ: 0.941004
Ran for 102550 frames Cell Events: 3903 mean_circ: 0.941001
Ran for 103050 frames Cell Events: 3929 mean_circ: 0.940955
Ran for 105050 frames Cell Events: 4013 mean_circ: 0.940988
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Ran for 121550 frames Cell Events: 4626 mean_circ: 0.940691
Ran for 122050 frames Cell Events: 4637 mean_circ: 0.940711
Ran for 122550 frames Cell Events: 4652 mean_circ: 0.940716
Ran for 123050 frames Cell Events: 4670 mean_circ: 0.94073
Ran for 123550 frames Cell Events: 4683 mean_circ: 0.940712
Ran for 124050 frames Cell Events: 4697 mean_circ: 0.940675
Ran for 124550 frames Cell Events: 4714 mean_circ: 0.940679
Ran for 125550 frames Cell Events: 4752 mean_circ: 0.940681
Ran for 126050 frames Cell Events: 4773 mean_circ: 0.940695
Ran for 127550 frames Cell Events: 4837 mean_circ: 0.940759
Ran for 128550 frames Cell Events: 4878 mean_circ: 0.940753
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Ran for 131050 frames Cell Events: 4965 mean_circ: 0.940628
Ran for 131550 frames Cell Events: 4979 mean_circ: 0.940631
Ran for 132050 frames Cell Events: 5000 mean_circ: 0.940582
Ran for 132550 frames Cell Events: 5020 mean_circ: 0.940524
Ran for 133550 frames Cell Events: 5068 mean_circ: 0.940553
Ran for 134050 frames Cell Events: 5079 mean_circ: 0.940549
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Ran for 143550 frames Cell Events: 5460 mean_circ: 0.940465
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Ran for 146550 frames Cell Events: 5593 mean_circ: 0.940312
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Ran for 189050 frames Cell Events: 7398 mean_circ: 0.940024
Ran for 189550 frames Cell Events: 7413 mean_circ: 0.940014
Ran for 190550 frames Cell Events: 7457 mean_circ: 0.940029
Ran for 191550 frames Cell Events: 7477 mean_circ: 0.94003
Ran for 192050 frames Cell Events: 7495 mean_circ: 0.939995
Ran for 192550 frames Cell Events: 7515 mean_circ: 0.93995
Ran for 193050 frames Cell Events: 7526 mean_circ: 0.939918
Ran for 193550 frames Cell Events: 7551 mean_circ: 0.93991
Ran for 194050 frames Cell Events: 7577 mean_circ: 0.939882
Ran for 194550 frames Cell Events: 7593 mean_circ: 0.939842
Ran for 195050 frames Cell Events: 7613 mean_circ: 0.939842
Ran for 195550 frames Cell Events: 7627 mean_circ: 0.93987
Ran for 196050 frames Cell Events: 7649 mean_circ: 0.939895
Ran for 196550 frames Cell Events: 7680 mean_circ: 0.939876
Ran for 197050 frames Cell Events: 7690 mean_circ: 0.939877
Ran for 197550 frames Cell Events: 7701 mean_circ: 0.93988
Ran for 198550 frames Cell Events: 7737 mean_circ: 0.939901
Ran for 199550 frames Cell Events: 7757 mean_circ: 0.939895
Ran for 200050 frames Cell Events: 7774 mean_circ: 0.939905
Ran for 200550 frames Cell Events: 7799 mean_circ: 0.939918
Ran for 201050 frames Cell Events: 7834 mean_circ: 0.939874
Ran for 201550 frames Cell Events: 7859 mean_circ: 0.939865
Ran for 203050 frames Cell Events: 7919 mean_circ: 0.939856
Ran for 203550 frames Cell Events: 7943 mean_circ: 0.939885
Ran for 204050 frames Cell Events: 7964 mean_circ: 0.939897
Ran for 204550 frames Cell Events: 7981 mean_circ: 0.939897
Ran for 205050 frames Cell Events: 8001 mean_circ: 0.9399
Ran for 205550 frames Cell Events: 8018 mean_circ: 0.939868
Ran for 206050 frames Cell Events: 8039 mean_circ: 0.939828
Ran for 206550 frames Cell Events: 8052 mean_circ: 0.939828
Ran for 207550 frames Cell Events: 8085 mean_circ: 0.93981
Ran for 208050 frames Cell Events: 8109 mean_circ: 0.939822
Ran for 208550 frames Cell Events: 8136 mean_circ: 0.939797
Ran for 209050 frames Cell Events: 8156 mean_circ: 0.939767
Ran for 209550 frames Cell Events: 8173 mean_circ: 0.939753
Ran for 210050 frames Cell Events: 8199 mean_circ: 0.939753
Ran for 210550 frames Cell Events: 8216 mean_circ: 0.939775
End this measurment..
Stopping time: 1.41653e+008
****************************************
Measurement finished
Mean Circularitiy: 0.939729
Mean Area: 607.307
Mean FL-1 intensity: 3099.4
Mean FL-2 intensity: 4185.67
Mean FL-3 intensity: 0
frames processed: 210733
Total Time: 105945ms
Number of Runs (new bg img) 0
Frames collected: 211796
Skipped frames: 16208
Rescued Frames: 1681766
Samples DAQ: 52950335
Samples written: 105897780
Samples per Channel: 52948890
FPS: 1999.11
Samples per second: 499776
Expected samples/s: 500000
Events: 8269
Events per second: 78.0498
jumped back (PMT): 14
#Frames read, frequency
# 0 frames :288524
# 1 frames :118616
# 2 frames :4430
# 3 frames :1808
# 4 frames :631
# 5 frames :2989
# 6 frames :1843
# 7 frames :1110
# 8 frames :542
# 9 frames :346
# 10 frames :874
# 11 frames :653
# 12 frames :345
# 13 frames :217
# 14 frames :151
# 15 frames :111
# 16 frames :86
# 17 frames :98
# 18 frames :62
# 19 frames :33
# 20 frames :32
# 21 frames :18
# 22 frames :17
# 23 frames :5
# 24 frames :9
# 25 frames :8
# 28 frames :3
# 29 frames :5
# 30 frames :4
# 31 frames :3
# 32 frames :1
# 35 frames :1
# 37 frames :1
# 38 frames :1
# 43 frames :1
# 44 frames :2
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6288
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0