Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_17.57.52
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "f473a0b451dd927f880958f94165881b",
"name": "M1_20170615_1853_dsRNA_W2_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:58:14"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8980 (max 8981) in '8980'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.5811
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.42986e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 23 mean_circ: 0.928786
Ran for 1550 frames Cell Events: 106 mean_circ: 0.938212
Ran for 3050 frames Cell Events: 149 mean_circ: 0.940438
Ran for 3550 frames Cell Events: 176 mean_circ: 0.938465
Ran for 4050 frames Cell Events: 207 mean_circ: 0.939053
Ran for 4550 frames Cell Events: 234 mean_circ: 0.937869
Ran for 5050 frames Cell Events: 267 mean_circ: 0.937909
Ran for 6050 frames Cell Events: 328 mean_circ: 0.93789
Ran for 7050 frames Cell Events: 391 mean_circ: 0.938535
Ran for 7550 frames Cell Events: 432 mean_circ: 0.938313
Ran for 9050 frames Cell Events: 537 mean_circ: 0.938204
Ran for 10050 frames Cell Events: 610 mean_circ: 0.938288
Ran for 10550 frames Cell Events: 647 mean_circ: 0.938468
Ran for 11050 frames Cell Events: 681 mean_circ: 0.938685
Ran for 12550 frames Cell Events: 788 mean_circ: 0.938755
Ran for 14050 frames Cell Events: 900 mean_circ: 0.939237
Ran for 16050 frames Cell Events: 998 mean_circ: 0.939447
Ran for 17050 frames Cell Events: 1063 mean_circ: 0.939382
Ran for 17550 frames Cell Events: 1088 mean_circ: 0.939218
Ran for 18050 frames Cell Events: 1117 mean_circ: 0.939083
Ran for 19550 frames Cell Events: 1209 mean_circ: 0.93934
Ran for 20050 frames Cell Events: 1234 mean_circ: 0.939453
Ran for 20550 frames Cell Events: 1279 mean_circ: 0.939448
Ran for 21050 frames Cell Events: 1321 mean_circ: 0.93959
Ran for 22550 frames Cell Events: 1416 mean_circ: 0.940009
Ran for 23550 frames Cell Events: 1488 mean_circ: 0.940226
Ran for 24550 frames Cell Events: 1563 mean_circ: 0.940255
Ran for 25050 frames Cell Events: 1595 mean_circ: 0.940162
Ran for 26050 frames Cell Events: 1658 mean_circ: 0.940348
Ran for 27050 frames Cell Events: 1723 mean_circ: 0.940571
Ran for 27550 frames Cell Events: 1758 mean_circ: 0.94071
Ran for 28050 frames Cell Events: 1801 mean_circ: 0.940875
Ran for 29050 frames Cell Events: 1882 mean_circ: 0.940934
Ran for 30050 frames Cell Events: 1965 mean_circ: 0.940703
Ran for 30550 frames Cell Events: 2000 mean_circ: 0.940903
Ran for 31050 frames Cell Events: 2034 mean_circ: 0.940954
Ran for 31550 frames Cell Events: 2061 mean_circ: 0.940925
Ran for 32050 frames Cell Events: 2095 mean_circ: 0.941038
Ran for 32550 frames Cell Events: 2123 mean_circ: 0.941058
Ran for 34050 frames Cell Events: 2193 mean_circ: 0.941116
Ran for 34550 frames Cell Events: 2211 mean_circ: 0.941076
Ran for 35550 frames Cell Events: 2270 mean_circ: 0.940882
Ran for 36050 frames Cell Events: 2297 mean_circ: 0.940795
Ran for 36550 frames Cell Events: 2334 mean_circ: 0.940817
Ran for 37050 frames Cell Events: 2369 mean_circ: 0.940952
Ran for 38550 frames Cell Events: 2447 mean_circ: 0.94095
Ran for 39050 frames Cell Events: 2479 mean_circ: 0.940968
Ran for 40050 frames Cell Events: 2558 mean_circ: 0.940924
Ran for 40550 frames Cell Events: 2580 mean_circ: 0.940883
Ran for 41050 frames Cell Events: 2618 mean_circ: 0.941028
Ran for 42050 frames Cell Events: 2696 mean_circ: 0.941104
Ran for 43050 frames Cell Events: 2795 mean_circ: 0.941188
Ran for 43550 frames Cell Events: 2826 mean_circ: 0.941168
Ran for 44550 frames Cell Events: 2896 mean_circ: 0.941155
Ran for 45050 frames Cell Events: 2938 mean_circ: 0.941115
Ran for 46550 frames Cell Events: 3051 mean_circ: 0.941378
Ran for 48050 frames Cell Events: 3142 mean_circ: 0.941394
Ran for 48550 frames Cell Events: 3165 mean_circ: 0.941428
Ran for 49050 frames Cell Events: 3207 mean_circ: 0.941564
Ran for 49550 frames Cell Events: 3250 mean_circ: 0.94163
Ran for 50050 frames Cell Events: 3284 mean_circ: 0.941676
Ran for 51550 frames Cell Events: 3411 mean_circ: 0.941591
Ran for 52050 frames Cell Events: 3448 mean_circ: 0.941494
Ran for 52550 frames Cell Events: 3483 mean_circ: 0.941559
Ran for 53550 frames Cell Events: 3541 mean_circ: 0.941531
Ran for 54550 frames Cell Events: 3604 mean_circ: 0.941476
Ran for 55050 frames Cell Events: 3638 mean_circ: 0.941402
Ran for 55550 frames Cell Events: 3666 mean_circ: 0.941419
Ran for 56050 frames Cell Events: 3724 mean_circ: 0.941388
Ran for 57050 frames Cell Events: 3801 mean_circ: 0.941331
Ran for 58050 frames Cell Events: 3872 mean_circ: 0.941201
Ran for 58550 frames Cell Events: 3905 mean_circ: 0.941075
Ran for 60050 frames Cell Events: 4062 mean_circ: 0.94111
Ran for 60550 frames Cell Events: 4084 mean_circ: 0.941083
Ran for 61050 frames Cell Events: 4125 mean_circ: 0.941126
Ran for 62050 frames Cell Events: 4208 mean_circ: 0.941268
Ran for 62550 frames Cell Events: 4230 mean_circ: 0.941317
Ran for 63050 frames Cell Events: 4273 mean_circ: 0.941255
Ran for 63550 frames Cell Events: 4319 mean_circ: 0.941255
Ran for 64550 frames Cell Events: 4384 mean_circ: 0.941285
Ran for 65550 frames Cell Events: 4461 mean_circ: 0.941247
Ran for 66050 frames Cell Events: 4482 mean_circ: 0.941172
Ran for 66550 frames Cell Events: 4509 mean_circ: 0.94113
Ran for 67050 frames Cell Events: 4538 mean_circ: 0.941053
Ran for 67550 frames Cell Events: 4564 mean_circ: 0.941043
Ran for 69050 frames Cell Events: 4674 mean_circ: 0.941141
Ran for 69550 frames Cell Events: 4701 mean_circ: 0.941139
Ran for 70050 frames Cell Events: 4721 mean_circ: 0.941178
Ran for 70550 frames Cell Events: 4753 mean_circ: 0.941108
Ran for 71050 frames Cell Events: 4785 mean_circ: 0.94112
Ran for 72550 frames Cell Events: 4907 mean_circ: 0.940983
Ran for 73550 frames Cell Events: 4961 mean_circ: 0.940871
Ran for 74050 frames Cell Events: 4982 mean_circ: 0.940873
Ran for 74550 frames Cell Events: 5011 mean_circ: 0.940887
Ran for 75050 frames Cell Events: 5067 mean_circ: 0.940889
Ran for 75550 frames Cell Events: 5111 mean_circ: 0.940643
Ran for 76050 frames Cell Events: 5132 mean_circ: 0.940623
Ran for 76550 frames Cell Events: 5166 mean_circ: 0.940631
Ran for 77050 frames Cell Events: 5205 mean_circ: 0.940631
Ran for 77550 frames Cell Events: 5245 mean_circ: 0.940589
Ran for 78050 frames Cell Events: 5290 mean_circ: 0.940529
Ran for 78550 frames Cell Events: 5321 mean_circ: 0.940511
Ran for 80050 frames Cell Events: 5422 mean_circ: 0.940488
Ran for 80550 frames Cell Events: 5468 mean_circ: 0.940531
Ran for 82550 frames Cell Events: 5608 mean_circ: 0.940544
Ran for 83050 frames Cell Events: 5649 mean_circ: 0.940572
Ran for 83550 frames Cell Events: 5670 mean_circ: 0.940569
Ran for 85050 frames Cell Events: 5762 mean_circ: 0.940521
Ran for 85550 frames Cell Events: 5785 mean_circ: 0.940476
Ran for 86050 frames Cell Events: 5810 mean_circ: 0.940472
Ran for 86550 frames Cell Events: 5846 mean_circ: 0.940395
Ran for 87050 frames Cell Events: 5874 mean_circ: 0.940378
Ran for 87550 frames Cell Events: 5908 mean_circ: 0.94037
Ran for 88050 frames Cell Events: 5938 mean_circ: 0.940402
Ran for 88550 frames Cell Events: 5950 mean_circ: 0.940387
Ran for 89550 frames Cell Events: 6011 mean_circ: 0.940336
Ran for 90050 frames Cell Events: 6043 mean_circ: 0.940325
Ran for 90550 frames Cell Events: 6079 mean_circ: 0.940333
Ran for 92550 frames Cell Events: 6259 mean_circ: 0.940351
Ran for 96550 frames Cell Events: 6591 mean_circ: 0.940175
Ran for 97550 frames Cell Events: 6654 mean_circ: 0.940084
Ran for 98050 frames Cell Events: 6693 mean_circ: 0.940139
Ran for 100550 frames Cell Events: 6827 mean_circ: 0.940174
Ran for 101550 frames Cell Events: 6889 mean_circ: 0.940208
Ran for 103050 frames Cell Events: 7013 mean_circ: 0.940266
Ran for 104050 frames Cell Events: 7078 mean_circ: 0.940265
Ran for 104550 frames Cell Events: 7117 mean_circ: 0.940254
Ran for 105550 frames Cell Events: 7192 mean_circ: 0.940217
Ran for 106550 frames Cell Events: 7265 mean_circ: 0.94025
Ran for 107050 frames Cell Events: 7298 mean_circ: 0.940211
Ran for 107550 frames Cell Events: 7319 mean_circ: 0.940208
Ran for 109550 frames Cell Events: 7431 mean_circ: 0.940148
Ran for 110050 frames Cell Events: 7472 mean_circ: 0.940148
Ran for 112050 frames Cell Events: 7612 mean_circ: 0.940174
Ran for 112550 frames Cell Events: 7632 mean_circ: 0.940181
Ran for 113050 frames Cell Events: 7659 mean_circ: 0.940211
Ran for 113550 frames Cell Events: 7675 mean_circ: 0.940182
Ran for 114550 frames Cell Events: 7736 mean_circ: 0.940205
Ran for 115550 frames Cell Events: 7801 mean_circ: 0.940196
Ran for 117050 frames Cell Events: 7900 mean_circ: 0.940242
Ran for 117550 frames Cell Events: 7922 mean_circ: 0.940233
Ran for 118050 frames Cell Events: 7946 mean_circ: 0.940234
Ran for 118550 frames Cell Events: 7975 mean_circ: 0.940287
Ran for 119550 frames Cell Events: 8021 mean_circ: 0.940301
Ran for 120050 frames Cell Events: 8041 mean_circ: 0.940292
Ran for 120550 frames Cell Events: 8062 mean_circ: 0.94033
Ran for 121050 frames Cell Events: 8097 mean_circ: 0.94035
Ran for 121550 frames Cell Events: 8133 mean_circ: 0.94037
Ran for 122550 frames Cell Events: 8197 mean_circ: 0.940388
Ran for 123550 frames Cell Events: 8259 mean_circ: 0.940278
Ran for 124050 frames Cell Events: 8282 mean_circ: 0.94027
Ran for 125550 frames Cell Events: 8360 mean_circ: 0.940284
Ran for 126050 frames Cell Events: 8390 mean_circ: 0.940255
Ran for 127050 frames Cell Events: 8454 mean_circ: 0.940222
Ran for 127550 frames Cell Events: 8487 mean_circ: 0.94025
Ran for 128050 frames Cell Events: 8522 mean_circ: 0.940227
Ran for 128550 frames Cell Events: 8551 mean_circ: 0.940177
Ran for 129050 frames Cell Events: 8581 mean_circ: 0.9401
Ran for 130050 frames Cell Events: 8639 mean_circ: 0.940079
Ran for 130550 frames Cell Events: 8675 mean_circ: 0.940016
Ran for 131050 frames Cell Events: 8708 mean_circ: 0.940014
Ran for 132550 frames Cell Events: 8804 mean_circ: 0.940032
Ran for 133050 frames Cell Events: 8841 mean_circ: 0.940042
Ran for 133550 frames Cell Events: 8871 mean_circ: 0.940035
Ran for 134550 frames Cell Events: 8931 mean_circ: 0.94002
Ran for 135050 frames Cell Events: 8963 mean_circ: 0.940006
End this measurment..
Stopping time: 1.43054e+008
****************************************
Measurement finished
Mean Circularitiy: 0.940037
Mean Area: 625.74
Mean FL-1 intensity: 3304.98
Mean FL-2 intensity: 3621.61
Mean FL-3 intensity: 0
frames processed: 133138
Total Time: 67772.1ms
Number of Runs (new bg img) 0
Frames collected: 135393
Skipped frames: 21874
Rescued Frames: 1996393
Samples DAQ: 33850076
Samples written: 67696384
Samples per Channel: 33848192
FPS: 1997.77
Samples per second: 499441
Expected samples/s: 500000
Events: 8981
Events per second: 132.518
jumped back (PMT): 11
#Frames read, frequency
# 0 frames :199183
# 1 frames :59023
# 2 frames :2487
# 3 frames :1145
# 4 frames :396
# 5 frames :3205
# 6 frames :1665
# 7 frames :1130
# 8 frames :599
# 9 frames :333
# 10 frames :268
# 11 frames :286
# 12 frames :237
# 13 frames :180
# 14 frames :116
# 15 frames :75
# 16 frames :74
# 17 frames :60
# 18 frames :52
# 19 frames :48
# 20 frames :30
# 21 frames :31
# 22 frames :19
# 23 frames :12
# 24 frames :18
# 25 frames :9
# 26 frames :6
# 27 frames :6
# 28 frames :15
# 29 frames :9
# 30 frames :7
# 31 frames :4
# 32 frames :3
# 33 frames :3
# 34 frames :1
# 35 frames :1
# 36 frames :2
# 38 frames :2
# 39 frames :1
# 40 frames :1
# 42 frames :3
# 45 frames :1
# 71 frames :1
# 92 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.5811
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0