Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_18.08.20
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "0fcea94726f7dde0d4b30815cd0ead0e",
"name": "M1_20170616_1355_dsRNA_O6_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "16:08:40"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8165 (max 8166) in '8165'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.83
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.11472e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 16 mean_circ: 0.947491
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Ran for 106550 frames Cell Events: 3010 mean_circ: 0.949757
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Ran for 124050 frames Cell Events: 3482 mean_circ: 0.949628
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Ran for 125050 frames Cell Events: 3510 mean_circ: 0.949658
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Ran for 126050 frames Cell Events: 3552 mean_circ: 0.949534
Ran for 126550 frames Cell Events: 3568 mean_circ: 0.949559
Ran for 127050 frames Cell Events: 3576 mean_circ: 0.949547
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Ran for 132050 frames Cell Events: 3700 mean_circ: 0.949458
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Ran for 194550 frames Cell Events: 5500 mean_circ: 0.950166
Ran for 195050 frames Cell Events: 5518 mean_circ: 0.950168
Ran for 196050 frames Cell Events: 5562 mean_circ: 0.950181
Ran for 196550 frames Cell Events: 5572 mean_circ: 0.950181
Ran for 197050 frames Cell Events: 5585 mean_circ: 0.950215
Ran for 198050 frames Cell Events: 5628 mean_circ: 0.95021
Ran for 198550 frames Cell Events: 5646 mean_circ: 0.950234
Ran for 199050 frames Cell Events: 5659 mean_circ: 0.950257
Ran for 199550 frames Cell Events: 5677 mean_circ: 0.95021
Ran for 203550 frames Cell Events: 5826 mean_circ: 0.950244
Ran for 204050 frames Cell Events: 5840 mean_circ: 0.950198
Ran for 204550 frames Cell Events: 5862 mean_circ: 0.950223
Ran for 205050 frames Cell Events: 5879 mean_circ: 0.950247
Ran for 205550 frames Cell Events: 5900 mean_circ: 0.950247
Ran for 206050 frames Cell Events: 5911 mean_circ: 0.950219
Ran for 206550 frames Cell Events: 5935 mean_circ: 0.950225
Ran for 207550 frames Cell Events: 5967 mean_circ: 0.950131
Ran for 208050 frames Cell Events: 5985 mean_circ: 0.950116
Ran for 208550 frames Cell Events: 5993 mean_circ: 0.950125
Ran for 209550 frames Cell Events: 6025 mean_circ: 0.950119
Ran for 210050 frames Cell Events: 6048 mean_circ: 0.950119
Ran for 212050 frames Cell Events: 6118 mean_circ: 0.950193
Ran for 213050 frames Cell Events: 6143 mean_circ: 0.950197
Ran for 213550 frames Cell Events: 6151 mean_circ: 0.950188
Ran for 214550 frames Cell Events: 6187 mean_circ: 0.95019
Ran for 215050 frames Cell Events: 6200 mean_circ: 0.950179
Ran for 215550 frames Cell Events: 6212 mean_circ: 0.950188
Ran for 216050 frames Cell Events: 6232 mean_circ: 0.950215
Ran for 216550 frames Cell Events: 6245 mean_circ: 0.95019
Ran for 217050 frames Cell Events: 6269 mean_circ: 0.9502
Ran for 217550 frames Cell Events: 6280 mean_circ: 0.950183
Ran for 218050 frames Cell Events: 6292 mean_circ: 0.950188
Ran for 218550 frames Cell Events: 6310 mean_circ: 0.950199
Ran for 219050 frames Cell Events: 6327 mean_circ: 0.95021
Ran for 219550 frames Cell Events: 6349 mean_circ: 0.950248
Ran for 220550 frames Cell Events: 6369 mean_circ: 0.950228
Ran for 221050 frames Cell Events: 6381 mean_circ: 0.950234
Ran for 221550 frames Cell Events: 6392 mean_circ: 0.950241
Ran for 222050 frames Cell Events: 6401 mean_circ: 0.950247
Ran for 222550 frames Cell Events: 6415 mean_circ: 0.950294
Ran for 223050 frames Cell Events: 6435 mean_circ: 0.950304
Ran for 223550 frames Cell Events: 6448 mean_circ: 0.950308
Ran for 224050 frames Cell Events: 6466 mean_circ: 0.950318
Ran for 224550 frames Cell Events: 6486 mean_circ: 0.950301
Ran for 225050 frames Cell Events: 6494 mean_circ: 0.950273
Ran for 225550 frames Cell Events: 6507 mean_circ: 0.95028
Ran for 226050 frames Cell Events: 6523 mean_circ: 0.950281
Ran for 226550 frames Cell Events: 6548 mean_circ: 0.950272
Ran for 227550 frames Cell Events: 6581 mean_circ: 0.95025
Ran for 228050 frames Cell Events: 6590 mean_circ: 0.950249
Ran for 228550 frames Cell Events: 6605 mean_circ: 0.950268
Ran for 229050 frames Cell Events: 6623 mean_circ: 0.950276
Ran for 230550 frames Cell Events: 6660 mean_circ: 0.95029
Ran for 231050 frames Cell Events: 6677 mean_circ: 0.950287
Ran for 231550 frames Cell Events: 6696 mean_circ: 0.950302
Ran for 232050 frames Cell Events: 6715 mean_circ: 0.950363
Ran for 232550 frames Cell Events: 6730 mean_circ: 0.950365
Ran for 233050 frames Cell Events: 6749 mean_circ: 0.95038
Ran for 234050 frames Cell Events: 6767 mean_circ: 0.950406
Ran for 235050 frames Cell Events: 6790 mean_circ: 0.950389
Ran for 236050 frames Cell Events: 6825 mean_circ: 0.950317
Ran for 237050 frames Cell Events: 6851 mean_circ: 0.950251
Ran for 237550 frames Cell Events: 6871 mean_circ: 0.95025
Ran for 238550 frames Cell Events: 6908 mean_circ: 0.950259
Ran for 239550 frames Cell Events: 6929 mean_circ: 0.950262
Ran for 240550 frames Cell Events: 6959 mean_circ: 0.950273
Ran for 242550 frames Cell Events: 7028 mean_circ: 0.950143
Ran for 243550 frames Cell Events: 7060 mean_circ: 0.95016
Ran for 244050 frames Cell Events: 7075 mean_circ: 0.950174
Ran for 244550 frames Cell Events: 7084 mean_circ: 0.950187
Ran for 246050 frames Cell Events: 7138 mean_circ: 0.950182
Ran for 246550 frames Cell Events: 7154 mean_circ: 0.950168
Ran for 247050 frames Cell Events: 7162 mean_circ: 0.950167
Ran for 248550 frames Cell Events: 7208 mean_circ: 0.950216
Ran for 249050 frames Cell Events: 7231 mean_circ: 0.950203
Ran for 250550 frames Cell Events: 7278 mean_circ: 0.950204
Ran for 251050 frames Cell Events: 7294 mean_circ: 0.950198
Ran for 252050 frames Cell Events: 7322 mean_circ: 0.950189
Ran for 252550 frames Cell Events: 7339 mean_circ: 0.950163
Ran for 253050 frames Cell Events: 7355 mean_circ: 0.950151
Ran for 253550 frames Cell Events: 7366 mean_circ: 0.950122
Ran for 254050 frames Cell Events: 7379 mean_circ: 0.950123
Ran for 254550 frames Cell Events: 7398 mean_circ: 0.950139
Ran for 255550 frames Cell Events: 7423 mean_circ: 0.950145
Ran for 256050 frames Cell Events: 7444 mean_circ: 0.950113
Ran for 257550 frames Cell Events: 7489 mean_circ: 0.950123
Ran for 258050 frames Cell Events: 7504 mean_circ: 0.950153
Ran for 258550 frames Cell Events: 7517 mean_circ: 0.950149
Ran for 259550 frames Cell Events: 7548 mean_circ: 0.950181
Ran for 260550 frames Cell Events: 7572 mean_circ: 0.950147
Ran for 261050 frames Cell Events: 7586 mean_circ: 0.950168
Ran for 261550 frames Cell Events: 7601 mean_circ: 0.950166
Ran for 262050 frames Cell Events: 7611 mean_circ: 0.95015
Ran for 262550 frames Cell Events: 7623 mean_circ: 0.950151
Ran for 263050 frames Cell Events: 7635 mean_circ: 0.950138
Ran for 263550 frames Cell Events: 7648 mean_circ: 0.950155
Ran for 264050 frames Cell Events: 7663 mean_circ: 0.950172
Ran for 264550 frames Cell Events: 7678 mean_circ: 0.950145
Ran for 265050 frames Cell Events: 7685 mean_circ: 0.950137
Ran for 266050 frames Cell Events: 7713 mean_circ: 0.950164
Ran for 267050 frames Cell Events: 7739 mean_circ: 0.950171
Ran for 267550 frames Cell Events: 7746 mean_circ: 0.950147
Ran for 268050 frames Cell Events: 7758 mean_circ: 0.950136
Ran for 268550 frames Cell Events: 7772 mean_circ: 0.950152
Ran for 269050 frames Cell Events: 7788 mean_circ: 0.950169
Ran for 269550 frames Cell Events: 7806 mean_circ: 0.950171
Ran for 270050 frames Cell Events: 7818 mean_circ: 0.95017
Ran for 271550 frames Cell Events: 7859 mean_circ: 0.950118
Ran for 272050 frames Cell Events: 7875 mean_circ: 0.950107
Ran for 272550 frames Cell Events: 7888 mean_circ: 0.950081
Ran for 273050 frames Cell Events: 7899 mean_circ: 0.950086
Ran for 273550 frames Cell Events: 7909 mean_circ: 0.95008
Ran for 274050 frames Cell Events: 7917 mean_circ: 0.950082
Ran for 274550 frames Cell Events: 7926 mean_circ: 0.950087
Ran for 275050 frames Cell Events: 7942 mean_circ: 0.950098
Ran for 275550 frames Cell Events: 7959 mean_circ: 0.950097
Ran for 276050 frames Cell Events: 7973 mean_circ: 0.950077
Ran for 276550 frames Cell Events: 7986 mean_circ: 0.950087
Ran for 277050 frames Cell Events: 8002 mean_circ: 0.95009
Ran for 277550 frames Cell Events: 8013 mean_circ: 0.95007
Ran for 278550 frames Cell Events: 8044 mean_circ: 0.950055
Ran for 279050 frames Cell Events: 8056 mean_circ: 0.950085
Ran for 280550 frames Cell Events: 8112 mean_circ: 0.950127
Ran for 281550 frames Cell Events: 8136 mean_circ: 0.950133
Ran for 282550 frames Cell Events: 8157 mean_circ: 0.950086
End this measurment..
Stopping time: 2.11613e+008
****************************************
Measurement finished
Mean Circularitiy: 0.950093
Mean Area: 566.51
Mean FL-1 intensity: 2898.95
Mean FL-2 intensity: 3844.46
Mean FL-3 intensity: 0
frames processed: 282177
Total Time: 141458ms
Number of Runs (new bg img) 0
Frames collected: 282787
Skipped frames: 3638
Rescued Frames: 832654
Samples DAQ: 70696954
Samples written: 141393264
Samples per Channel: 70696632
FPS: 1999.09
Samples per second: 499771
Expected samples/s: 500000
Events: 8166
Events per second: 57.7273
jumped back (PMT): 5450
#Frames read, frequency
# 0 frames :399824
# 1 frames :145320
# 2 frames :5613
# 3 frames :2476
# 4 frames :1361
# 5 frames :738
# 6 frames :993
# 7 frames :1669
# 8 frames :1419
# 9 frames :1135
# 10 frames :1211
# 11 frames :902
# 12 frames :601
# 13 frames :378
# 14 frames :294
# 15 frames :242
# 16 frames :238
# 17 frames :179
# 18 frames :173
# 19 frames :142
# 20 frames :115
# 21 frames :97
# 22 frames :73
# 23 frames :49
# 24 frames :38
# 25 frames :34
# 26 frames :25
# 27 frames :20
# 28 frames :17
# 29 frames :18
# 30 frames :19
# 31 frames :18
# 32 frames :10
# 33 frames :12
# 34 frames :9
# 35 frames :7
# 36 frames :10
# 37 frames :4
# 38 frames :6
# 39 frames :4
# 40 frames :5
# 41 frames :1
# 42 frames :1
# 44 frames :2
# 45 frames :3
# 46 frames :1
# 47 frames :4
# 49 frames :3
# 50 frames :2
# 51 frames :1
# 52 frames :1
# 54 frames :1
# 56 frames :1
# 60 frames :1
# 61 frames :1
# 64 frames :2
# 65 frames :1
# 83 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.83
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0