Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_18.14.01
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "af8b806df384066106a88f90556a97d4",
"name": "M1_20170616_1520_dsRNA_N5_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "16:14:22"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8083 (max 8084) in '8083'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0753
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.16565e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 12 mean_circ: 0.968077
Ran for 1050 frames Cell Events: 36 mean_circ: 0.954687
Ran for 2050 frames Cell Events: 65 mean_circ: 0.95425
Ran for 3550 frames Cell Events: 110 mean_circ: 0.952975
Ran for 4550 frames Cell Events: 139 mean_circ: 0.952419
Ran for 5050 frames Cell Events: 153 mean_circ: 0.95304
Ran for 5550 frames Cell Events: 159 mean_circ: 0.953176
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Ran for 86050 frames Cell Events: 2652 mean_circ: 0.952378
Ran for 86550 frames Cell Events: 2669 mean_circ: 0.952338
Ran for 87550 frames Cell Events: 2700 mean_circ: 0.952375
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Ran for 91050 frames Cell Events: 2806 mean_circ: 0.952201
Ran for 91550 frames Cell Events: 2815 mean_circ: 0.952219
Ran for 92550 frames Cell Events: 2848 mean_circ: 0.952109
Ran for 93050 frames Cell Events: 2859 mean_circ: 0.952044
Ran for 93550 frames Cell Events: 2870 mean_circ: 0.952004
Ran for 94050 frames Cell Events: 2888 mean_circ: 0.951854
Ran for 94550 frames Cell Events: 2906 mean_circ: 0.951862
Ran for 95050 frames Cell Events: 2919 mean_circ: 0.951872
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Ran for 96550 frames Cell Events: 2964 mean_circ: 0.951845
Ran for 98050 frames Cell Events: 3013 mean_circ: 0.951711
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Ran for 100550 frames Cell Events: 3088 mean_circ: 0.951475
Ran for 102050 frames Cell Events: 3139 mean_circ: 0.951357
Ran for 102550 frames Cell Events: 3155 mean_circ: 0.951324
Ran for 103050 frames Cell Events: 3172 mean_circ: 0.951281
Ran for 104050 frames Cell Events: 3205 mean_circ: 0.951304
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Ran for 107550 frames Cell Events: 3330 mean_circ: 0.951202
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Ran for 121550 frames Cell Events: 3787 mean_circ: 0.951292
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Ran for 123550 frames Cell Events: 3872 mean_circ: 0.951232
Ran for 124550 frames Cell Events: 3906 mean_circ: 0.951272
Ran for 125050 frames Cell Events: 3927 mean_circ: 0.951272
Ran for 126050 frames Cell Events: 3959 mean_circ: 0.951268
Ran for 127050 frames Cell Events: 3985 mean_circ: 0.951247
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Ran for 131550 frames Cell Events: 4115 mean_circ: 0.951404
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Ran for 132550 frames Cell Events: 4137 mean_circ: 0.951366
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Ran for 143550 frames Cell Events: 4470 mean_circ: 0.951337
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Ran for 146550 frames Cell Events: 4553 mean_circ: 0.951365
Ran for 147050 frames Cell Events: 4572 mean_circ: 0.951324
Ran for 148550 frames Cell Events: 4639 mean_circ: 0.951221
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Ran for 202050 frames Cell Events: 6668 mean_circ: 0.95107
Ran for 204050 frames Cell Events: 6727 mean_circ: 0.951116
Ran for 204550 frames Cell Events: 6738 mean_circ: 0.95113
Ran for 205050 frames Cell Events: 6750 mean_circ: 0.95115
Ran for 207050 frames Cell Events: 6809 mean_circ: 0.95107
Ran for 208050 frames Cell Events: 6849 mean_circ: 0.951068
Ran for 209050 frames Cell Events: 6892 mean_circ: 0.951108
Ran for 210050 frames Cell Events: 6920 mean_circ: 0.951115
Ran for 210550 frames Cell Events: 6938 mean_circ: 0.951139
Ran for 211050 frames Cell Events: 6947 mean_circ: 0.951123
Ran for 211550 frames Cell Events: 6965 mean_circ: 0.951138
Ran for 212550 frames Cell Events: 7009 mean_circ: 0.951146
Ran for 213050 frames Cell Events: 7026 mean_circ: 0.951106
Ran for 214050 frames Cell Events: 7057 mean_circ: 0.951111
Ran for 214550 frames Cell Events: 7075 mean_circ: 0.951149
Ran for 215050 frames Cell Events: 7087 mean_circ: 0.951123
Ran for 215550 frames Cell Events: 7100 mean_circ: 0.951132
Ran for 216050 frames Cell Events: 7112 mean_circ: 0.951146
Ran for 216550 frames Cell Events: 7129 mean_circ: 0.951158
Ran for 217050 frames Cell Events: 7144 mean_circ: 0.951182
Ran for 217550 frames Cell Events: 7162 mean_circ: 0.951219
Ran for 218050 frames Cell Events: 7186 mean_circ: 0.95123
Ran for 218550 frames Cell Events: 7197 mean_circ: 0.951219
Ran for 219050 frames Cell Events: 7209 mean_circ: 0.951234
Ran for 219550 frames Cell Events: 7229 mean_circ: 0.951217
Ran for 220550 frames Cell Events: 7256 mean_circ: 0.951216
Ran for 221050 frames Cell Events: 7278 mean_circ: 0.951245
Ran for 221550 frames Cell Events: 7302 mean_circ: 0.951249
Ran for 224050 frames Cell Events: 7385 mean_circ: 0.951224
Ran for 225050 frames Cell Events: 7421 mean_circ: 0.951201
Ran for 225550 frames Cell Events: 7435 mean_circ: 0.95121
Ran for 226050 frames Cell Events: 7442 mean_circ: 0.951213
Ran for 228550 frames Cell Events: 7502 mean_circ: 0.951226
Ran for 229050 frames Cell Events: 7517 mean_circ: 0.951203
Ran for 229550 frames Cell Events: 7538 mean_circ: 0.951216
Ran for 230050 frames Cell Events: 7567 mean_circ: 0.951199
Ran for 230550 frames Cell Events: 7580 mean_circ: 0.951217
Ran for 231050 frames Cell Events: 7600 mean_circ: 0.951153
Ran for 232050 frames Cell Events: 7682 mean_circ: 0.951091
Ran for 233050 frames Cell Events: 7722 mean_circ: 0.951026
Ran for 233550 frames Cell Events: 7735 mean_circ: 0.951003
Ran for 235050 frames Cell Events: 7786 mean_circ: 0.951046
Ran for 235550 frames Cell Events: 7808 mean_circ: 0.95101
Ran for 236050 frames Cell Events: 7832 mean_circ: 0.951025
Ran for 236550 frames Cell Events: 7840 mean_circ: 0.951001
Ran for 237050 frames Cell Events: 7855 mean_circ: 0.951009
Ran for 237550 frames Cell Events: 7870 mean_circ: 0.95101
Ran for 240050 frames Cell Events: 7958 mean_circ: 0.95104
Ran for 241050 frames Cell Events: 8001 mean_circ: 0.951032
Ran for 241550 frames Cell Events: 8021 mean_circ: 0.951022
Ran for 242050 frames Cell Events: 8035 mean_circ: 0.951044
Ran for 243050 frames Cell Events: 8070 mean_circ: 0.951084
End this measurment..
Stopping time: 2.16687e+008
****************************************
Measurement finished
Mean Circularitiy: 0.951068
Mean Area: 599.437
Mean FL-1 intensity: 3354.61
Mean FL-2 intensity: 4181.77
Mean FL-3 intensity: 0
frames processed: 240660
Total Time: 121769ms
Number of Runs (new bg img) 0
Frames collected: 243341
Skipped frames: 22303
Rescued Frames: 2192838
Samples DAQ: 60835563
Samples written: 121670330
Samples per Channel: 60835165
FPS: 1998.38
Samples per second: 499595
Expected samples/s: 500000
Events: 8084
Events per second: 66.388
jumped back (PMT): 4806
#Frames read, frequency
# 0 frames :353595
# 1 frames :115131
# 2 frames :4687
# 3 frames :1967
# 4 frames :1171
# 5 frames :617
# 6 frames :1223
# 7 frames :1551
# 8 frames :1179
# 9 frames :771
# 10 frames :590
# 11 frames :1000
# 12 frames :649
# 13 frames :421
# 14 frames :286
# 15 frames :238
# 16 frames :196
# 17 frames :201
# 18 frames :180
# 19 frames :121
# 20 frames :117
# 21 frames :112
# 22 frames :76
# 23 frames :62
# 24 frames :47
# 25 frames :37
# 26 frames :41
# 27 frames :34
# 28 frames :23
# 29 frames :23
# 30 frames :19
# 31 frames :12
# 32 frames :9
# 33 frames :16
# 34 frames :9
# 35 frames :10
# 36 frames :11
# 37 frames :11
# 38 frames :5
# 39 frames :3
# 40 frames :6
# 41 frames :2
# 42 frames :3
# 43 frames :2
# 44 frames :2
# 45 frames :3
# 47 frames :7
# 48 frames :1
# 49 frames :4
# 50 frames :2
# 52 frames :2
# 53 frames :2
# 62 frames :1
# 64 frames :1
# 68 frames :1
# 76 frames :1
# 93 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0753
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0