M1_20170616_1614_dsRNA_N7_col.rtdc
Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_18.17.40
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}dclab-tdms2rtdc
{"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "52d82d6f992d55d05b50bc9a143bb308",
"name": "M1_20170616_1614_dsRNA_N7_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "16:18:01"
}
}
FG_Config.mcf
[Kamerasektion]ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.8228
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.19842e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 0 mean_circ: 0.934725
Ran for 550 frames Cell Events: 7 mean_circ: 0.955979
Ran for 1550 frames Cell Events: 23 mean_circ: 0.938084
Ran for 2050 frames Cell Events: 29 mean_circ: 0.929851
Ran for 2550 frames Cell Events: 39 mean_circ: 0.92295
Ran for 3050 frames Cell Events: 46 mean_circ: 0.925474
Ran for 3550 frames Cell Events: 54 mean_circ: 0.924278
Ran for 4050 frames Cell Events: 62 mean_circ: 0.924977
Ran for 5050 frames Cell Events: 95 mean_circ: 0.931955
Ran for 5550 frames Cell Events: 105 mean_circ: 0.933678
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Ran for 6550 frames Cell Events: 119 mean_circ: 0.932253
Ran for 7050 frames Cell Events: 133 mean_circ: 0.930684
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Ran for 20050 frames Cell Events: 335 mean_circ: 0.929625
Ran for 21050 frames Cell Events: 350 mean_circ: 0.93008
Ran for 21550 frames Cell Events: 352 mean_circ: 0.930275
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Ran for 22550 frames Cell Events: 378 mean_circ: 0.931106
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Ran for 30550 frames Cell Events: 573 mean_circ: 0.932402
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Ran for 31550 frames Cell Events: 590 mean_circ: 0.932745
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Ran for 32550 frames Cell Events: 601 mean_circ: 0.933121
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Ran for 33550 frames Cell Events: 615 mean_circ: 0.933371
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Ran for 35550 frames Cell Events: 638 mean_circ: 0.93356
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Ran for 72550 frames Cell Events: 1214 mean_circ: 0.931843
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Ran for 76550 frames Cell Events: 1265 mean_circ: 0.931744
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Ran for 81050 frames Cell Events: 1335 mean_circ: 0.932147
Ran for 81550 frames Cell Events: 1355 mean_circ: 0.93215
Ran for 82050 frames Cell Events: 1372 mean_circ: 0.932258
Ran for 82550 frames Cell Events: 1379 mean_circ: 0.932361
Ran for 83050 frames Cell Events: 1384 mean_circ: 0.932418
Ran for 83550 frames Cell Events: 1394 mean_circ: 0.932308
Ran for 84050 frames Cell Events: 1404 mean_circ: 0.932229
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Ran for 118050 frames Cell Events: 1951 mean_circ: 0.931364
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Ran for 121550 frames Cell Events: 1993 mean_circ: 0.93102
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Ran for 159550 frames Cell Events: 2687 mean_circ: 0.930366
Ran for 160050 frames Cell Events: 2696 mean_circ: 0.930383
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Ran for 379550 frames Cell Events: 6563 mean_circ: 0.930872
Ran for 380050 frames Cell Events: 6572 mean_circ: 0.930889
Ran for 380550 frames Cell Events: 6581 mean_circ: 0.93082
Ran for 381050 frames Cell Events: 6588 mean_circ: 0.930793
Ran for 381550 frames Cell Events: 6598 mean_circ: 0.930793
Ran for 382050 frames Cell Events: 6609 mean_circ: 0.930785
Ran for 383050 frames Cell Events: 6624 mean_circ: 0.930791
Ran for 383550 frames Cell Events: 6631 mean_circ: 0.9307
Ran for 384050 frames Cell Events: 6635 mean_circ: 0.930729
Ran for 384550 frames Cell Events: 6642 mean_circ: 0.930725
Ran for 385050 frames Cell Events: 6649 mean_circ: 0.930691
Ran for 386050 frames Cell Events: 6669 mean_circ: 0.930599
Ran for 386550 frames Cell Events: 6675 mean_circ: 0.930618
Ran for 387050 frames Cell Events: 6688 mean_circ: 0.93058
Ran for 387550 frames Cell Events: 6690 mean_circ: 0.930583
Ran for 388050 frames Cell Events: 6703 mean_circ: 0.930609
Ran for 388550 frames Cell Events: 6713 mean_circ: 0.930604
Ran for 389050 frames Cell Events: 6718 mean_circ: 0.930569
Ran for 389550 frames Cell Events: 6728 mean_circ: 0.930591
Ran for 390050 frames Cell Events: 6739 mean_circ: 0.930593
Ran for 391050 frames Cell Events: 6764 mean_circ: 0.930567
Ran for 391550 frames Cell Events: 6770 mean_circ: 0.930561
Ran for 392050 frames Cell Events: 6778 mean_circ: 0.930585
Ran for 393550 frames Cell Events: 6796 mean_circ: 0.93059
Ran for 394050 frames Cell Events: 6802 mean_circ: 0.930556
Ran for 394550 frames Cell Events: 6807 mean_circ: 0.93057
Ran for 395050 frames Cell Events: 6815 mean_circ: 0.930554
Ran for 395550 frames Cell Events: 6824 mean_circ: 0.930556
Ran for 396050 frames Cell Events: 6837 mean_circ: 0.930518
Ran for 396550 frames Cell Events: 6840 mean_circ: 0.930522
Ran for 397050 frames Cell Events: 6847 mean_circ: 0.930532
Ran for 397550 frames Cell Events: 6853 mean_circ: 0.93053
Ran for 398050 frames Cell Events: 6857 mean_circ: 0.93052
Ran for 398550 frames Cell Events: 6868 mean_circ: 0.9305
Ran for 399050 frames Cell Events: 6874 mean_circ: 0.930485
Ran for 399550 frames Cell Events: 6884 mean_circ: 0.930493
Ran for 400050 frames Cell Events: 6888 mean_circ: 0.930493
Ran for 401550 frames Cell Events: 6914 mean_circ: 0.930538
Ran for 402050 frames Cell Events: 6916 mean_circ: 0.93055
Ran for 402550 frames Cell Events: 6927 mean_circ: 0.930573
Ran for 403050 frames Cell Events: 6936 mean_circ: 0.930569
Ran for 404550 frames Cell Events: 6957 mean_circ: 0.930541
Ran for 405050 frames Cell Events: 6967 mean_circ: 0.930537
Ran for 406550 frames Cell Events: 6990 mean_circ: 0.930535
Ran for 407050 frames Cell Events: 6996 mean_circ: 0.930529
Ran for 407550 frames Cell Events: 7000 mean_circ: 0.930526
Ran for 408550 frames Cell Events: 7015 mean_circ: 0.93053
Ran for 409050 frames Cell Events: 7024 mean_circ: 0.930537
Ran for 409550 frames Cell Events: 7032 mean_circ: 0.930551
Ran for 410050 frames Cell Events: 7039 mean_circ: 0.930567
Ran for 411050 frames Cell Events: 7050 mean_circ: 0.930572
Ran for 411550 frames Cell Events: 7063 mean_circ: 0.930579
Ran for 412050 frames Cell Events: 7073 mean_circ: 0.930589
Ran for 412550 frames Cell Events: 7081 mean_circ: 0.930571
Ran for 413050 frames Cell Events: 7090 mean_circ: 0.930563
Ran for 413550 frames Cell Events: 7101 mean_circ: 0.930607
Ran for 414050 frames Cell Events: 7108 mean_circ: 0.930629
Ran for 414550 frames Cell Events: 7115 mean_circ: 0.930646
Ran for 415050 frames Cell Events: 7118 mean_circ: 0.930664
Ran for 415550 frames Cell Events: 7128 mean_circ: 0.930654
Ran for 416050 frames Cell Events: 7141 mean_circ: 0.930627
Ran for 416550 frames Cell Events: 7146 mean_circ: 0.930615
Ran for 417550 frames Cell Events: 7170 mean_circ: 0.930629
Ran for 418050 frames Cell Events: 7176 mean_circ: 0.930631
Ran for 418550 frames Cell Events: 7189 mean_circ: 0.930655
Ran for 419050 frames Cell Events: 7194 mean_circ: 0.930662
Ran for 419550 frames Cell Events: 7205 mean_circ: 0.93067
Ran for 420050 frames Cell Events: 7214 mean_circ: 0.930673
Ran for 420550 frames Cell Events: 7227 mean_circ: 0.930705
Ran for 421050 frames Cell Events: 7236 mean_circ: 0.930731
Ran for 421550 frames Cell Events: 7247 mean_circ: 0.930724
Ran for 422550 frames Cell Events: 7259 mean_circ: 0.930697
Ran for 423050 frames Cell Events: 7269 mean_circ: 0.93072
Ran for 423550 frames Cell Events: 7275 mean_circ: 0.930743
Ran for 424050 frames Cell Events: 7285 mean_circ: 0.930765
Ran for 424550 frames Cell Events: 7295 mean_circ: 0.930797
Ran for 425050 frames Cell Events: 7302 mean_circ: 0.930796
Ran for 425550 frames Cell Events: 7317 mean_circ: 0.930794
Ran for 426050 frames Cell Events: 7328 mean_circ: 0.930809
Ran for 426550 frames Cell Events: 7334 mean_circ: 0.930819
Ran for 427050 frames Cell Events: 7351 mean_circ: 0.930803
Ran for 427550 frames Cell Events: 7360 mean_circ: 0.930791
Ran for 428050 frames Cell Events: 7371 mean_circ: 0.930798
Ran for 428550 frames Cell Events: 7381 mean_circ: 0.930784
Ran for 429050 frames Cell Events: 7389 mean_circ: 0.930783
Ran for 429550 frames Cell Events: 7398 mean_circ: 0.930806
Ran for 430050 frames Cell Events: 7407 mean_circ: 0.930806
Ran for 430550 frames Cell Events: 7414 mean_circ: 0.930809
Ran for 431050 frames Cell Events: 7421 mean_circ: 0.930821
Ran for 431550 frames Cell Events: 7428 mean_circ: 0.930833
Ran for 432050 frames Cell Events: 7437 mean_circ: 0.930844
Ran for 432550 frames Cell Events: 7448 mean_circ: 0.930831
Ran for 433050 frames Cell Events: 7454 mean_circ: 0.930848
Ran for 433550 frames Cell Events: 7460 mean_circ: 0.930861
Ran for 434050 frames Cell Events: 7466 mean_circ: 0.930869
Ran for 434550 frames Cell Events: 7471 mean_circ: 0.930838
Ran for 435050 frames Cell Events: 7483 mean_circ: 0.930839
Ran for 435550 frames Cell Events: 7493 mean_circ: 0.93083
Ran for 436050 frames Cell Events: 7502 mean_circ: 0.930826
Ran for 436550 frames Cell Events: 7506 mean_circ: 0.930826
Ran for 437050 frames Cell Events: 7516 mean_circ: 0.930841
Ran for 438050 frames Cell Events: 7526 mean_circ: 0.930795
Ran for 438550 frames Cell Events: 7531 mean_circ: 0.930799
Ran for 439050 frames Cell Events: 7541 mean_circ: 0.930791
Ran for 439550 frames Cell Events: 7552 mean_circ: 0.930774
Ran for 440050 frames Cell Events: 7560 mean_circ: 0.930764
Ran for 440550 frames Cell Events: 7568 mean_circ: 0.930791
Ran for 441050 frames Cell Events: 7575 mean_circ: 0.930822
Ran for 442050 frames Cell Events: 7590 mean_circ: 0.930794
Ran for 442550 frames Cell Events: 7600 mean_circ: 0.930812
Ran for 443050 frames Cell Events: 7605 mean_circ: 0.930829
Ran for 443550 frames Cell Events: 7612 mean_circ: 0.930805
Ran for 444050 frames Cell Events: 7619 mean_circ: 0.930772
Ran for 445550 frames Cell Events: 7644 mean_circ: 0.930693
Ran for 446050 frames Cell Events: 7651 mean_circ: 0.930668
Ran for 447050 frames Cell Events: 7672 mean_circ: 0.930636
Ran for 447550 frames Cell Events: 7676 mean_circ: 0.930643
Ran for 448050 frames Cell Events: 7682 mean_circ: 0.930657
Ran for 448550 frames Cell Events: 7690 mean_circ: 0.930618
Ran for 449050 frames Cell Events: 7697 mean_circ: 0.930635
Ran for 449550 frames Cell Events: 7704 mean_circ: 0.930594
Ran for 450050 frames Cell Events: 7714 mean_circ: 0.930585
Ran for 450550 frames Cell Events: 7723 mean_circ: 0.930594
Ran for 451050 frames Cell Events: 7735 mean_circ: 0.930623
Ran for 451550 frames Cell Events: 7741 mean_circ: 0.930639
Ran for 452050 frames Cell Events: 7751 mean_circ: 0.930653
Ran for 452550 frames Cell Events: 7759 mean_circ: 0.930609
Ran for 453050 frames Cell Events: 7765 mean_circ: 0.930626
Ran for 454050 frames Cell Events: 7782 mean_circ: 0.930594
Ran for 454550 frames Cell Events: 7789 mean_circ: 0.930601
Ran for 455550 frames Cell Events: 7812 mean_circ: 0.930606
Ran for 456050 frames Cell Events: 7823 mean_circ: 0.930606
Ran for 457550 frames Cell Events: 7844 mean_circ: 0.930573
Ran for 458050 frames Cell Events: 7856 mean_circ: 0.930579
Ran for 458550 frames Cell Events: 7865 mean_circ: 0.930571
Ran for 459050 frames Cell Events: 7870 mean_circ: 0.930558
Ran for 459550 frames Cell Events: 7880 mean_circ: 0.930565
Ran for 460050 frames Cell Events: 7890 mean_circ: 0.930531
Ran for 460550 frames Cell Events: 7897 mean_circ: 0.930555
Ran for 461550 frames Cell Events: 7922 mean_circ: 0.930515
Ran for 462050 frames Cell Events: 7927 mean_circ: 0.930525
Ran for 462550 frames Cell Events: 7931 mean_circ: 0.930491
Ran for 463050 frames Cell Events: 7941 mean_circ: 0.930447
Ran for 464050 frames Cell Events: 7959 mean_circ: 0.930451
Ran for 464550 frames Cell Events: 7965 mean_circ: 0.930467
Ran for 465050 frames Cell Events: 7976 mean_circ: 0.930456
Ran for 465550 frames Cell Events: 7983 mean_circ: 0.930466
Ran for 466050 frames Cell Events: 7994 mean_circ: 0.930475
Ran for 466550 frames Cell Events: 8005 mean_circ: 0.930489
Ran for 467550 frames Cell Events: 8023 mean_circ: 0.930501
Ran for 468050 frames Cell Events: 8030 mean_circ: 0.930443
Ran for 469050 frames Cell Events: 8049 mean_circ: 0.930394
Ran for 469550 frames Cell Events: 8059 mean_circ: 0.930403
Ran for 470050 frames Cell Events: 8068 mean_circ: 0.930411
Ran for 470550 frames Cell Events: 8085 mean_circ: 0.930374
Ran for 471050 frames Cell Events: 8092 mean_circ: 0.930386
Ran for 471550 frames Cell Events: 8104 mean_circ: 0.930373
Ran for 472050 frames Cell Events: 8111 mean_circ: 0.930344
Ran for 473050 frames Cell Events: 8135 mean_circ: 0.930312
Ran for 473550 frames Cell Events: 8141 mean_circ: 0.930298
Ran for 474050 frames Cell Events: 8151 mean_circ: 0.930317
Ran for 474550 frames Cell Events: 8159 mean_circ: 0.930315
End this measurment..
Stopping time: 2.2008e+008
****************************************
Measurement finished
Mean Circularitiy: 0.930312
Mean Area: 710.269
Mean FL-1 intensity: 3057.51
Mean FL-2 intensity: 3822.05
Mean FL-3 intensity: 0
frames processed: 473713
Total Time: 237466ms
Number of Runs (new bg img) 0
Frames collected: 474800
Skipped frames: 33103
Rescued Frames: 2979249
Samples DAQ: 118700013
Samples written: 237399782
Samples per Channel: 118699891
FPS: 1999.44
Samples per second: 499860
Expected samples/s: 500000
Events: 8161
Events per second: 34.367
jumped back (PMT): 7093
#Frames read, frequency
# 0 frames :602558
# 1 frames :321235
# 2 frames :7654
# 3 frames :3832
# 4 frames :1401
# 5 frames :985
# 6 frames :1507
# 7 frames :2297
# 8 frames :1072
# 9 frames :715
# 10 frames :1755
# 11 frames :2473
# 12 frames :972
# 13 frames :307
# 14 frames :152
# 15 frames :138
# 16 frames :125
# 17 frames :97
# 18 frames :74
# 19 frames :38
# 20 frames :39
# 21 frames :23
# 22 frames :27
# 23 frames :21
# 24 frames :18
# 25 frames :8
# 26 frames :9
# 27 frames :8
# 28 frames :9
# 29 frames :4
# 30 frames :2
# 31 frames :4
# 32 frames :4
# 33 frames :3
# 34 frames :2
# 35 frames :2
# 36 frames :1
# 37 frames :1
# 38 frames :3
# 40 frames :1
# 43 frames :1
# 45 frames :1
# 50 frames :1
# 52 frames :1
M1_para.ini
[General]Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.8228
parameters.txt
#fRT-DC Configuration file# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0
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Additional Information
Field | Value |
---|---|
license | Creative Commons Public Domain Dedication |
created | 3 months ago |
etag | c35761ddd2ab2a3027177f5d969fb93e |
format | RT-FDC |
id | 7ad98059-4cfa-42c7-b4ee-8fe14868390b |
metadata modified | 3 months ago |
mimetype | RT-DC |
package id | a527d179-7904-445c-8749-7dd63ffc8c4a |
position | 0 |
s3 available | True |
s3 url | https://objectstore.hpccloud.mpcdf.mpg.de/circle-8a23b5b7-6d86-46b7-9833-62373e9ec110/resource/7ad/980/59-4cfa-42c7-b4ee-8fe14868390b |
sha256 | df4ab3040e4391c6ca507ef048efcac9f1d499e2b2f5658ff47ff59cc8c4f5c8 |
size | 141.8 MiB |
dc:experiment:date | 2017-06-16 |
dc:experiment:event count | 8,160 |
dc:experiment:run identifier | None-9c91 |
dc:experiment:run index | 1 |
dc:experiment:sample | 20170616_1614_dsRNA_N7_col |
dc:experiment:time | 16:14:55 |
dc:fluorescence:bit depth | 16 |
dc:fluorescence:channel 1 name | FL1 |
dc:fluorescence:channel 2 name | FL2 |
dc:fluorescence:channel count | 2 |
dc:fluorescence:channels installed | 3 |
dc:fluorescence:laser 1 lambda | 488 |
dc:fluorescence:laser 1 power | 10 |
dc:fluorescence:laser 2 lambda | 561 |
dc:fluorescence:laser 2 power | 35 |
dc:fluorescence:laser 3 lambda | 640 |
dc:fluorescence:laser 3 power | 0 |
dc:fluorescence:laser count | 2 |
dc:fluorescence:lasers installed | 3 |
dc:fluorescence:sample rate | 500,000 |
dc:fluorescence:samples per event | 300 |
dc:fluorescence:signal max | 1 |
dc:fluorescence:signal min | -1 |
dc:fluorescence:trace median | 5 |
dc:imaging:flash device | LED (undefined) |
dc:imaging:flash duration | 2 |
dc:imaging:frame rate | 2,000 |
dc:imaging:pixel size | 0.34 |
dc:imaging:roi position x | 504 |
dc:imaging:roi position y | 472 |
dc:imaging:roi size x | 256 |
dc:imaging:roi size y | 96 |
dc:online contour:bin area min | 50 |
dc:online contour:bin kernel | 5 |
dc:online contour:bin threshold | 6 |
dc:online contour:image blur | 0 |
dc:online contour:no absdiff | True |
dc:setup:channel width | 20 |
dc:setup:chip region | channel |
dc:setup:flow rate | 0.04 |
dc:setup:module composition | Cell_Flow_2, Fluor |
dc:setup:software version | fRT-DC V1.06170206 | dclab 0.60.3 |
sp:cells:cell type | KC167 |
sp:cells:live | True |
sp:cells:organism | Drosophila melanogaster |
sp:chip:channel width | 20 |
sp:experiment:buffer osmolality | 325 |
sp:experiment:buffer ph | 6.8 |
sp:experiment:concentration | 5 |
sp:identifiers:labels | GFP tdTomato |
sp:identifiers:tags | NLS-GFP |