Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_18.29.17
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "2a36d3f2ffc89d197bece4159a89f9e6",
"name": "M1_20170616_1859_dsRNA_p5_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "16:29:38"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8362 (max 8363) in '8362'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9512
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.29698e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 32 mean_circ: 0.938579
Ran for 1550 frames Cell Events: 43 mean_circ: 0.933409
Ran for 2050 frames Cell Events: 61 mean_circ: 0.935747
Ran for 2550 frames Cell Events: 71 mean_circ: 0.937882
Ran for 3050 frames Cell Events: 83 mean_circ: 0.937715
Ran for 3550 frames Cell Events: 103 mean_circ: 0.939451
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Ran for 5050 frames Cell Events: 147 mean_circ: 0.940415
Ran for 5550 frames Cell Events: 158 mean_circ: 0.940787
Ran for 6550 frames Cell Events: 182 mean_circ: 0.940553
Ran for 7050 frames Cell Events: 193 mean_circ: 0.940286
Ran for 8050 frames Cell Events: 218 mean_circ: 0.940798
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Ran for 67050 frames Cell Events: 1966 mean_circ: 0.935874
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Ran for 72550 frames Cell Events: 2157 mean_circ: 0.935544
Ran for 73050 frames Cell Events: 2169 mean_circ: 0.935568
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Ran for 83050 frames Cell Events: 2496 mean_circ: 0.935898
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Ran for 84050 frames Cell Events: 2536 mean_circ: 0.935928
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Ran for 86550 frames Cell Events: 2625 mean_circ: 0.935919
Ran for 87550 frames Cell Events: 2665 mean_circ: 0.936004
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Ran for 90050 frames Cell Events: 2739 mean_circ: 0.936233
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Ran for 91050 frames Cell Events: 2771 mean_circ: 0.936238
Ran for 92550 frames Cell Events: 2814 mean_circ: 0.936344
Ran for 93050 frames Cell Events: 2826 mean_circ: 0.936383
Ran for 93550 frames Cell Events: 2836 mean_circ: 0.936391
Ran for 94050 frames Cell Events: 2851 mean_circ: 0.936358
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Ran for 97050 frames Cell Events: 2930 mean_circ: 0.936176
Ran for 97550 frames Cell Events: 2940 mean_circ: 0.936111
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Ran for 100050 frames Cell Events: 3004 mean_circ: 0.936214
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Ran for 101550 frames Cell Events: 3050 mean_circ: 0.936104
Ran for 102050 frames Cell Events: 3062 mean_circ: 0.936118
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Ran for 107050 frames Cell Events: 3217 mean_circ: 0.936124
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Ran for 122050 frames Cell Events: 3727 mean_circ: 0.93605
Ran for 122550 frames Cell Events: 3746 mean_circ: 0.936036
Ran for 123050 frames Cell Events: 3774 mean_circ: 0.935891
Ran for 123550 frames Cell Events: 3792 mean_circ: 0.935885
Ran for 124050 frames Cell Events: 3814 mean_circ: 0.935897
Ran for 124550 frames Cell Events: 3827 mean_circ: 0.93593
Ran for 125550 frames Cell Events: 3863 mean_circ: 0.935883
Ran for 126550 frames Cell Events: 3896 mean_circ: 0.935944
Ran for 127050 frames Cell Events: 3904 mean_circ: 0.935971
Ran for 127550 frames Cell Events: 3916 mean_circ: 0.935962
Ran for 128550 frames Cell Events: 3950 mean_circ: 0.935932
Ran for 129050 frames Cell Events: 3966 mean_circ: 0.9359
Ran for 130050 frames Cell Events: 4003 mean_circ: 0.935972
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Ran for 131550 frames Cell Events: 4046 mean_circ: 0.935851
Ran for 132050 frames Cell Events: 4059 mean_circ: 0.935875
Ran for 132550 frames Cell Events: 4067 mean_circ: 0.935907
Ran for 133050 frames Cell Events: 4090 mean_circ: 0.93593
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Ran for 136050 frames Cell Events: 4196 mean_circ: 0.936081
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Ran for 142050 frames Cell Events: 4398 mean_circ: 0.935993
Ran for 143550 frames Cell Events: 4436 mean_circ: 0.935956
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Ran for 147050 frames Cell Events: 4554 mean_circ: 0.935905
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Ran for 148050 frames Cell Events: 4580 mean_circ: 0.935938
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Ran for 172550 frames Cell Events: 5383 mean_circ: 0.935903
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Ran for 177050 frames Cell Events: 5536 mean_circ: 0.935911
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Ran for 182050 frames Cell Events: 5722 mean_circ: 0.935798
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Ran for 189550 frames Cell Events: 5941 mean_circ: 0.935725
Ran for 190050 frames Cell Events: 5950 mean_circ: 0.935725
Ran for 190550 frames Cell Events: 5971 mean_circ: 0.935739
Ran for 191050 frames Cell Events: 5984 mean_circ: 0.935725
Ran for 192550 frames Cell Events: 6037 mean_circ: 0.935745
Ran for 193050 frames Cell Events: 6057 mean_circ: 0.93574
Ran for 195050 frames Cell Events: 6141 mean_circ: 0.935653
Ran for 195550 frames Cell Events: 6157 mean_circ: 0.935652
Ran for 197550 frames Cell Events: 6217 mean_circ: 0.935651
Ran for 198050 frames Cell Events: 6237 mean_circ: 0.935651
Ran for 198550 frames Cell Events: 6252 mean_circ: 0.935643
Ran for 199050 frames Cell Events: 6258 mean_circ: 0.935598
Ran for 200550 frames Cell Events: 6299 mean_circ: 0.935603
Ran for 201050 frames Cell Events: 6311 mean_circ: 0.935594
Ran for 201550 frames Cell Events: 6332 mean_circ: 0.935625
Ran for 203050 frames Cell Events: 6398 mean_circ: 0.935542
Ran for 203550 frames Cell Events: 6417 mean_circ: 0.935518
Ran for 204050 frames Cell Events: 6434 mean_circ: 0.935535
Ran for 204550 frames Cell Events: 6444 mean_circ: 0.935537
Ran for 208050 frames Cell Events: 6563 mean_circ: 0.935415
Ran for 208550 frames Cell Events: 6574 mean_circ: 0.935404
Ran for 210050 frames Cell Events: 6619 mean_circ: 0.935454
Ran for 210550 frames Cell Events: 6627 mean_circ: 0.935402
Ran for 211050 frames Cell Events: 6642 mean_circ: 0.935374
Ran for 211550 frames Cell Events: 6657 mean_circ: 0.935386
Ran for 212050 frames Cell Events: 6671 mean_circ: 0.935423
Ran for 213050 frames Cell Events: 6699 mean_circ: 0.935426
Ran for 214550 frames Cell Events: 6749 mean_circ: 0.935453
Ran for 215050 frames Cell Events: 6763 mean_circ: 0.935452
Ran for 216050 frames Cell Events: 6805 mean_circ: 0.935465
Ran for 216550 frames Cell Events: 6821 mean_circ: 0.935498
Ran for 218050 frames Cell Events: 6872 mean_circ: 0.935436
Ran for 218550 frames Cell Events: 6887 mean_circ: 0.935398
Ran for 219050 frames Cell Events: 6913 mean_circ: 0.935398
Ran for 219550 frames Cell Events: 6926 mean_circ: 0.935442
Ran for 220050 frames Cell Events: 6950 mean_circ: 0.935445
Ran for 220550 frames Cell Events: 6971 mean_circ: 0.935437
Ran for 221050 frames Cell Events: 6984 mean_circ: 0.935426
Ran for 222050 frames Cell Events: 7023 mean_circ: 0.935469
Ran for 222550 frames Cell Events: 7038 mean_circ: 0.935462
Ran for 223050 frames Cell Events: 7078 mean_circ: 0.935439
Ran for 224050 frames Cell Events: 7115 mean_circ: 0.935487
Ran for 224550 frames Cell Events: 7137 mean_circ: 0.935475
Ran for 225050 frames Cell Events: 7148 mean_circ: 0.935478
Ran for 226050 frames Cell Events: 7188 mean_circ: 0.935486
Ran for 227050 frames Cell Events: 7217 mean_circ: 0.935535
Ran for 227550 frames Cell Events: 7225 mean_circ: 0.935531
Ran for 229050 frames Cell Events: 7270 mean_circ: 0.935511
Ran for 229550 frames Cell Events: 7286 mean_circ: 0.9355
Ran for 230050 frames Cell Events: 7298 mean_circ: 0.935538
Ran for 230550 frames Cell Events: 7321 mean_circ: 0.935547
Ran for 231050 frames Cell Events: 7341 mean_circ: 0.935572
Ran for 232550 frames Cell Events: 7393 mean_circ: 0.935578
Ran for 233050 frames Cell Events: 7412 mean_circ: 0.935572
Ran for 234050 frames Cell Events: 7449 mean_circ: 0.935591
Ran for 234550 frames Cell Events: 7462 mean_circ: 0.93556
Ran for 235050 frames Cell Events: 7481 mean_circ: 0.935567
Ran for 236050 frames Cell Events: 7515 mean_circ: 0.935561
Ran for 236550 frames Cell Events: 7534 mean_circ: 0.935551
Ran for 237550 frames Cell Events: 7583 mean_circ: 0.935513
Ran for 238550 frames Cell Events: 7619 mean_circ: 0.935432
Ran for 240050 frames Cell Events: 7662 mean_circ: 0.935374
Ran for 240550 frames Cell Events: 7671 mean_circ: 0.935393
Ran for 241050 frames Cell Events: 7685 mean_circ: 0.935389
Ran for 241550 frames Cell Events: 7697 mean_circ: 0.935371
Ran for 242050 frames Cell Events: 7704 mean_circ: 0.935376
Ran for 242550 frames Cell Events: 7716 mean_circ: 0.935338
Ran for 243550 frames Cell Events: 7753 mean_circ: 0.935422
Ran for 244050 frames Cell Events: 7765 mean_circ: 0.935442
Ran for 244550 frames Cell Events: 7778 mean_circ: 0.93545
Ran for 245050 frames Cell Events: 7796 mean_circ: 0.935467
Ran for 245550 frames Cell Events: 7810 mean_circ: 0.935458
Ran for 246050 frames Cell Events: 7831 mean_circ: 0.935473
Ran for 246550 frames Cell Events: 7835 mean_circ: 0.935497
Ran for 247550 frames Cell Events: 7857 mean_circ: 0.93547
Ran for 248050 frames Cell Events: 7871 mean_circ: 0.935473
Ran for 248550 frames Cell Events: 7897 mean_circ: 0.93544
Ran for 249550 frames Cell Events: 7937 mean_circ: 0.935501
Ran for 250050 frames Cell Events: 7953 mean_circ: 0.935502
Ran for 250550 frames Cell Events: 7969 mean_circ: 0.93553
Ran for 251050 frames Cell Events: 7981 mean_circ: 0.935524
Ran for 251550 frames Cell Events: 8002 mean_circ: 0.935536
Ran for 252050 frames Cell Events: 8016 mean_circ: 0.935559
Ran for 252550 frames Cell Events: 8033 mean_circ: 0.935501
Ran for 253550 frames Cell Events: 8062 mean_circ: 0.935511
Ran for 254550 frames Cell Events: 8090 mean_circ: 0.935498
Ran for 256050 frames Cell Events: 8150 mean_circ: 0.935527
Ran for 257550 frames Cell Events: 8187 mean_circ: 0.935562
Ran for 258050 frames Cell Events: 8209 mean_circ: 0.935587
Ran for 258550 frames Cell Events: 8229 mean_circ: 0.935574
Ran for 259050 frames Cell Events: 8242 mean_circ: 0.935596
Ran for 259550 frames Cell Events: 8262 mean_circ: 0.935597
Ran for 260550 frames Cell Events: 8287 mean_circ: 0.93562
Ran for 261050 frames Cell Events: 8302 mean_circ: 0.935628
Ran for 262050 frames Cell Events: 8334 mean_circ: 0.935554
End this measurment..
Stopping time: 2.2983e+008
****************************************
Measurement finished
Mean Circularitiy: 0.935554
Mean Area: 644.502
Mean FL-1 intensity: 3093.63
Mean FL-2 intensity: 4289.9
Mean FL-3 intensity: 0
frames processed: 261784
Total Time: 131593ms
Number of Runs (new bg img) 0
Frames collected: 262987
Skipped frames: 66645
Rescued Frames: 5527820
Samples DAQ: 65746692
Samples written: 131493042
Samples per Channel: 65746521
FPS: 1998.48
Samples per second: 499619
Expected samples/s: 500000
Events: 8363
Events per second: 63.5518
jumped back (PMT): 22
#Frames read, frequency
# 0 frames :379078
# 1 frames :127351
# 2 frames :5065
# 3 frames :2241
# 4 frames :1395
# 5 frames :682
# 6 frames :1309
# 7 frames :1724
# 8 frames :1302
# 9 frames :853
# 10 frames :863
# 11 frames :981
# 12 frames :679
# 13 frames :503
# 14 frames :354
# 15 frames :262
# 16 frames :199
# 17 frames :172
# 18 frames :141
# 19 frames :125
# 20 frames :119
# 21 frames :90
# 22 frames :78
# 23 frames :62
# 24 frames :43
# 25 frames :28
# 26 frames :35
# 27 frames :28
# 28 frames :21
# 29 frames :19
# 30 frames :19
# 31 frames :18
# 32 frames :16
# 33 frames :22
# 34 frames :8
# 35 frames :6
# 36 frames :6
# 37 frames :5
# 38 frames :5
# 39 frames :7
# 40 frames :6
# 41 frames :5
# 42 frames :3
# 44 frames :2
# 45 frames :2
# 50 frames :3
# 51 frames :1
# 52 frames :1
# 54 frames :1
# 57 frames :3
# 65 frames :2
# 87 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9512
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0