Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_18.40.40
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "8af7ec11b9cbaafa88d75e62754edae2",
"name": "M1_20170618_1415_dsRNA_R7_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "16:41:01"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8366 (max 8367) in '8366'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9366
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.85462e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 11 mean_circ: 0.963198
Ran for 1050 frames Cell Events: 30 mean_circ: 0.949094
Ran for 1550 frames Cell Events: 48 mean_circ: 0.950268
Ran for 2050 frames Cell Events: 64 mean_circ: 0.952794
Ran for 2550 frames Cell Events: 80 mean_circ: 0.952928
Ran for 3050 frames Cell Events: 93 mean_circ: 0.953666
Ran for 3550 frames Cell Events: 107 mean_circ: 0.953447
Ran for 4050 frames Cell Events: 120 mean_circ: 0.953106
Ran for 5050 frames Cell Events: 149 mean_circ: 0.952817
Ran for 5550 frames Cell Events: 163 mean_circ: 0.953084
Ran for 6050 frames Cell Events: 171 mean_circ: 0.953531
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Ran for 9550 frames Cell Events: 290 mean_circ: 0.95283
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Ran for 13550 frames Cell Events: 441 mean_circ: 0.953568
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Ran for 73550 frames Cell Events: 2503 mean_circ: 0.953437
Ran for 74050 frames Cell Events: 2526 mean_circ: 0.953547
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Ran for 76550 frames Cell Events: 2638 mean_circ: 0.953365
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Ran for 77550 frames Cell Events: 2669 mean_circ: 0.953346
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Ran for 80050 frames Cell Events: 2763 mean_circ: 0.953433
Ran for 80550 frames Cell Events: 2776 mean_circ: 0.953475
Ran for 81050 frames Cell Events: 2798 mean_circ: 0.953475
Ran for 81550 frames Cell Events: 2816 mean_circ: 0.953393
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Ran for 82550 frames Cell Events: 2863 mean_circ: 0.953472
Ran for 83050 frames Cell Events: 2883 mean_circ: 0.953461
Ran for 84050 frames Cell Events: 2925 mean_circ: 0.95351
Ran for 84550 frames Cell Events: 2951 mean_circ: 0.953485
Ran for 85050 frames Cell Events: 2968 mean_circ: 0.953506
Ran for 85550 frames Cell Events: 2980 mean_circ: 0.953512
Ran for 86050 frames Cell Events: 3003 mean_circ: 0.953535
Ran for 86550 frames Cell Events: 3024 mean_circ: 0.953544
Ran for 87050 frames Cell Events: 3040 mean_circ: 0.953565
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Ran for 91050 frames Cell Events: 3200 mean_circ: 0.953665
Ran for 91550 frames Cell Events: 3215 mean_circ: 0.953687
Ran for 92050 frames Cell Events: 3232 mean_circ: 0.953697
Ran for 92550 frames Cell Events: 3256 mean_circ: 0.953691
Ran for 93050 frames Cell Events: 3276 mean_circ: 0.953676
Ran for 93550 frames Cell Events: 3294 mean_circ: 0.953626
Ran for 94050 frames Cell Events: 3309 mean_circ: 0.953563
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Ran for 97050 frames Cell Events: 3428 mean_circ: 0.95364
Ran for 97550 frames Cell Events: 3441 mean_circ: 0.953668
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Ran for 102550 frames Cell Events: 3684 mean_circ: 0.95367
Ran for 103050 frames Cell Events: 3709 mean_circ: 0.953656
Ran for 103550 frames Cell Events: 3724 mean_circ: 0.953671
Ran for 104050 frames Cell Events: 3750 mean_circ: 0.95369
Ran for 107050 frames Cell Events: 3868 mean_circ: 0.953774
Ran for 110050 frames Cell Events: 4009 mean_circ: 0.953734
Ran for 110550 frames Cell Events: 4026 mean_circ: 0.953725
Ran for 111050 frames Cell Events: 4054 mean_circ: 0.953651
Ran for 112050 frames Cell Events: 4087 mean_circ: 0.953587
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Ran for 118050 frames Cell Events: 4350 mean_circ: 0.953606
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Ran for 121550 frames Cell Events: 4500 mean_circ: 0.953274
Ran for 122050 frames Cell Events: 4516 mean_circ: 0.953321
Ran for 122550 frames Cell Events: 4530 mean_circ: 0.953328
Ran for 123050 frames Cell Events: 4547 mean_circ: 0.953321
Ran for 123550 frames Cell Events: 4558 mean_circ: 0.953318
Ran for 124050 frames Cell Events: 4580 mean_circ: 0.95332
Ran for 125550 frames Cell Events: 4653 mean_circ: 0.953286
Ran for 126050 frames Cell Events: 4672 mean_circ: 0.953276
Ran for 126550 frames Cell Events: 4695 mean_circ: 0.953271
Ran for 127550 frames Cell Events: 4730 mean_circ: 0.953289
Ran for 128550 frames Cell Events: 4782 mean_circ: 0.953227
Ran for 129050 frames Cell Events: 4794 mean_circ: 0.953249
Ran for 129550 frames Cell Events: 4812 mean_circ: 0.953306
Ran for 130050 frames Cell Events: 4828 mean_circ: 0.953317
Ran for 130550 frames Cell Events: 4842 mean_circ: 0.953352
Ran for 132050 frames Cell Events: 4906 mean_circ: 0.953387
Ran for 132550 frames Cell Events: 4929 mean_circ: 0.953379
Ran for 133050 frames Cell Events: 4948 mean_circ: 0.953407
Ran for 134050 frames Cell Events: 4989 mean_circ: 0.953424
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Ran for 167550 frames Cell Events: 6446 mean_circ: 0.953131
Ran for 168050 frames Cell Events: 6471 mean_circ: 0.953138
Ran for 168550 frames Cell Events: 6495 mean_circ: 0.95313
Ran for 169550 frames Cell Events: 6534 mean_circ: 0.953143
Ran for 170550 frames Cell Events: 6570 mean_circ: 0.953136
Ran for 171550 frames Cell Events: 6620 mean_circ: 0.953076
Ran for 173050 frames Cell Events: 6684 mean_circ: 0.953051
Ran for 174050 frames Cell Events: 6725 mean_circ: 0.953008
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Ran for 179550 frames Cell Events: 6976 mean_circ: 0.952861
Ran for 180050 frames Cell Events: 6999 mean_circ: 0.952842
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Ran for 191050 frames Cell Events: 7467 mean_circ: 0.952835
Ran for 191550 frames Cell Events: 7499 mean_circ: 0.952876
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Ran for 194550 frames Cell Events: 7647 mean_circ: 0.952916
Ran for 195050 frames Cell Events: 7668 mean_circ: 0.952927
Ran for 195550 frames Cell Events: 7687 mean_circ: 0.952941
Ran for 196550 frames Cell Events: 7726 mean_circ: 0.952935
Ran for 199050 frames Cell Events: 7837 mean_circ: 0.952914
Ran for 199550 frames Cell Events: 7863 mean_circ: 0.952928
Ran for 200050 frames Cell Events: 7876 mean_circ: 0.952936
Ran for 200550 frames Cell Events: 7897 mean_circ: 0.952933
Ran for 201050 frames Cell Events: 7907 mean_circ: 0.952939
Ran for 201550 frames Cell Events: 7928 mean_circ: 0.952947
Ran for 202550 frames Cell Events: 7971 mean_circ: 0.952917
Ran for 203050 frames Cell Events: 7987 mean_circ: 0.952928
Ran for 203550 frames Cell Events: 8001 mean_circ: 0.952909
Ran for 204050 frames Cell Events: 8021 mean_circ: 0.952923
Ran for 205050 frames Cell Events: 8058 mean_circ: 0.952919
Ran for 206050 frames Cell Events: 8117 mean_circ: 0.952939
Ran for 207050 frames Cell Events: 8170 mean_circ: 0.952967
Ran for 207550 frames Cell Events: 8205 mean_circ: 0.952983
Ran for 208050 frames Cell Events: 8228 mean_circ: 0.952992
Ran for 208550 frames Cell Events: 8253 mean_circ: 0.953001
Ran for 210050 frames Cell Events: 8326 mean_circ: 0.952937
Ran for 210550 frames Cell Events: 8355 mean_circ: 0.952932
End this measurment..
Stopping time: 3.85567e+008
****************************************
Measurement finished
Mean Circularitiy: 0.952946
Mean Area: 570.233
Mean FL-1 intensity: 2695.15
Mean FL-2 intensity: 2968.3
Mean FL-3 intensity: 0
frames processed: 210634
Total Time: 105491ms
Number of Runs (new bg img) 0
Frames collected: 210805
Skipped frames: 6303
Rescued Frames: 920804
Samples DAQ: 52702858
Samples written: 105402462
Samples per Channel: 52701231
FPS: 1998.33
Samples per second: 499581
Expected samples/s: 500000
Events: 8367
Events per second: 79.315
jumped back (PMT): 5805
#Frames read, frequency
# 0 frames :301009
# 1 frames :103345
# 2 frames :5288
# 3 frames :1575
# 4 frames :973
# 5 frames :390
# 6 frames :1720
# 7 frames :2088
# 8 frames :950
# 9 frames :543
# 10 frames :1326
# 11 frames :518
# 12 frames :377
# 13 frames :250
# 14 frames :188
# 15 frames :206
# 16 frames :160
# 17 frames :128
# 18 frames :102
# 19 frames :65
# 20 frames :47
# 21 frames :54
# 22 frames :54
# 23 frames :43
# 24 frames :25
# 25 frames :22
# 26 frames :12
# 27 frames :15
# 28 frames :16
# 29 frames :9
# 30 frames :5
# 31 frames :11
# 32 frames :4
# 33 frames :2
# 34 frames :1
# 35 frames :4
# 36 frames :2
# 37 frames :2
# 38 frames :2
# 39 frames :3
# 40 frames :2
# 41 frames :1
# 42 frames :1
# 44 frames :3
# 45 frames :1
# 48 frames :1
# 49 frames :1
# 51 frames :1
# 53 frames :1
# 67 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9366
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0