Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_18.47.24
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "781169c021e58eab2d381230f4292b08",
"name": "M1_20170618_1541_dsRNA_R8_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "16:47:48"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9094 (max 9095) in '9094'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.924
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.90662e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 11 mean_circ: 0.943664
Ran for 1550 frames Cell Events: 36 mean_circ: 0.942839
Ran for 3550 frames Cell Events: 105 mean_circ: 0.944107
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Ran for 84050 frames Cell Events: 2650 mean_circ: 0.946244
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Ran for 85050 frames Cell Events: 2689 mean_circ: 0.946373
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Ran for 87050 frames Cell Events: 2745 mean_circ: 0.946338
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Ran for 92050 frames Cell Events: 2885 mean_circ: 0.946458
Ran for 92550 frames Cell Events: 2900 mean_circ: 0.946473
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Ran for 93550 frames Cell Events: 2931 mean_circ: 0.946491
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Ran for 121550 frames Cell Events: 3871 mean_circ: 0.946721
Ran for 122050 frames Cell Events: 3898 mean_circ: 0.946676
Ran for 123050 frames Cell Events: 3933 mean_circ: 0.946581
Ran for 123550 frames Cell Events: 3946 mean_circ: 0.946581
Ran for 124050 frames Cell Events: 3955 mean_circ: 0.9466
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Ran for 125050 frames Cell Events: 3981 mean_circ: 0.946663
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Ran for 127550 frames Cell Events: 4074 mean_circ: 0.946709
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Ran for 187550 frames Cell Events: 6054 mean_circ: 0.946778
Ran for 188050 frames Cell Events: 6063 mean_circ: 0.946744
Ran for 188550 frames Cell Events: 6079 mean_circ: 0.946751
Ran for 189050 frames Cell Events: 6093 mean_circ: 0.946759
Ran for 189550 frames Cell Events: 6112 mean_circ: 0.946753
Ran for 191050 frames Cell Events: 6171 mean_circ: 0.946723
Ran for 191550 frames Cell Events: 6189 mean_circ: 0.946737
Ran for 192050 frames Cell Events: 6211 mean_circ: 0.946756
Ran for 192550 frames Cell Events: 6231 mean_circ: 0.94675
Ran for 193050 frames Cell Events: 6256 mean_circ: 0.946682
Ran for 193550 frames Cell Events: 6270 mean_circ: 0.946682
Ran for 194550 frames Cell Events: 6307 mean_circ: 0.946682
Ran for 195050 frames Cell Events: 6327 mean_circ: 0.94663
Ran for 195550 frames Cell Events: 6340 mean_circ: 0.946613
Ran for 196550 frames Cell Events: 6368 mean_circ: 0.946638
Ran for 197050 frames Cell Events: 6383 mean_circ: 0.946659
Ran for 198050 frames Cell Events: 6425 mean_circ: 0.946657
Ran for 198550 frames Cell Events: 6439 mean_circ: 0.94667
Ran for 199050 frames Cell Events: 6451 mean_circ: 0.946672
Ran for 199550 frames Cell Events: 6466 mean_circ: 0.946679
Ran for 200050 frames Cell Events: 6484 mean_circ: 0.946658
Ran for 200550 frames Cell Events: 6496 mean_circ: 0.946594
Ran for 201550 frames Cell Events: 6521 mean_circ: 0.946583
Ran for 202050 frames Cell Events: 6537 mean_circ: 0.94661
Ran for 202550 frames Cell Events: 6565 mean_circ: 0.94659
Ran for 203550 frames Cell Events: 6602 mean_circ: 0.946657
Ran for 204550 frames Cell Events: 6639 mean_circ: 0.946676
Ran for 205050 frames Cell Events: 6655 mean_circ: 0.94671
Ran for 206050 frames Cell Events: 6685 mean_circ: 0.946722
Ran for 206550 frames Cell Events: 6702 mean_circ: 0.946729
Ran for 207550 frames Cell Events: 6730 mean_circ: 0.946755
Ran for 208050 frames Cell Events: 6757 mean_circ: 0.946761
Ran for 208550 frames Cell Events: 6774 mean_circ: 0.946796
Ran for 209050 frames Cell Events: 6789 mean_circ: 0.9468
Ran for 209550 frames Cell Events: 6809 mean_circ: 0.946803
Ran for 210050 frames Cell Events: 6834 mean_circ: 0.946844
Ran for 210550 frames Cell Events: 6852 mean_circ: 0.946863
Ran for 211050 frames Cell Events: 6876 mean_circ: 0.946877
Ran for 211550 frames Cell Events: 6897 mean_circ: 0.946893
Ran for 212550 frames Cell Events: 6932 mean_circ: 0.946855
Ran for 214050 frames Cell Events: 6991 mean_circ: 0.9469
Ran for 214550 frames Cell Events: 7018 mean_circ: 0.946941
Ran for 215550 frames Cell Events: 7066 mean_circ: 0.946907
Ran for 216050 frames Cell Events: 7079 mean_circ: 0.946881
Ran for 216550 frames Cell Events: 7097 mean_circ: 0.94691
Ran for 218050 frames Cell Events: 7169 mean_circ: 0.946919
Ran for 219550 frames Cell Events: 7236 mean_circ: 0.946875
Ran for 220050 frames Cell Events: 7260 mean_circ: 0.946879
Ran for 220550 frames Cell Events: 7277 mean_circ: 0.946902
Ran for 221550 frames Cell Events: 7296 mean_circ: 0.946911
Ran for 222050 frames Cell Events: 7307 mean_circ: 0.946922
Ran for 222550 frames Cell Events: 7321 mean_circ: 0.946946
Ran for 223050 frames Cell Events: 7334 mean_circ: 0.946935
Ran for 224050 frames Cell Events: 7366 mean_circ: 0.946926
Ran for 224550 frames Cell Events: 7386 mean_circ: 0.946886
Ran for 225050 frames Cell Events: 7401 mean_circ: 0.946885
Ran for 227050 frames Cell Events: 7463 mean_circ: 0.946872
Ran for 227550 frames Cell Events: 7481 mean_circ: 0.946883
Ran for 228050 frames Cell Events: 7496 mean_circ: 0.946899
Ran for 228550 frames Cell Events: 7510 mean_circ: 0.946917
Ran for 229050 frames Cell Events: 7532 mean_circ: 0.946925
Ran for 230050 frames Cell Events: 7562 mean_circ: 0.946942
Ran for 231050 frames Cell Events: 7595 mean_circ: 0.946931
Ran for 232050 frames Cell Events: 7651 mean_circ: 0.946967
Ran for 233050 frames Cell Events: 7684 mean_circ: 0.946944
Ran for 233550 frames Cell Events: 7706 mean_circ: 0.946922
Ran for 234050 frames Cell Events: 7718 mean_circ: 0.946917
Ran for 234550 frames Cell Events: 7736 mean_circ: 0.946911
Ran for 235050 frames Cell Events: 7749 mean_circ: 0.946911
Ran for 235550 frames Cell Events: 7771 mean_circ: 0.946921
Ran for 236050 frames Cell Events: 7784 mean_circ: 0.946924
Ran for 236550 frames Cell Events: 7791 mean_circ: 0.946932
Ran for 237050 frames Cell Events: 7807 mean_circ: 0.946944
Ran for 237550 frames Cell Events: 7828 mean_circ: 0.946921
Ran for 238550 frames Cell Events: 7856 mean_circ: 0.946897
Ran for 239050 frames Cell Events: 7870 mean_circ: 0.946905
Ran for 239550 frames Cell Events: 7885 mean_circ: 0.946901
Ran for 240050 frames Cell Events: 7899 mean_circ: 0.946903
Ran for 240550 frames Cell Events: 7918 mean_circ: 0.946905
Ran for 241050 frames Cell Events: 7933 mean_circ: 0.946907
Ran for 241550 frames Cell Events: 7953 mean_circ: 0.946918
Ran for 242050 frames Cell Events: 7967 mean_circ: 0.946913
Ran for 244050 frames Cell Events: 8027 mean_circ: 0.946909
Ran for 244550 frames Cell Events: 8049 mean_circ: 0.946909
Ran for 245550 frames Cell Events: 8094 mean_circ: 0.946861
Ran for 246050 frames Cell Events: 8106 mean_circ: 0.946849
Ran for 246550 frames Cell Events: 8124 mean_circ: 0.946864
Ran for 247050 frames Cell Events: 8143 mean_circ: 0.946836
Ran for 249550 frames Cell Events: 8234 mean_circ: 0.946846
Ran for 250050 frames Cell Events: 8245 mean_circ: 0.946853
Ran for 250550 frames Cell Events: 8256 mean_circ: 0.946855
Ran for 251050 frames Cell Events: 8271 mean_circ: 0.946871
Ran for 251550 frames Cell Events: 8280 mean_circ: 0.946868
Ran for 252050 frames Cell Events: 8300 mean_circ: 0.946847
Ran for 252550 frames Cell Events: 8310 mean_circ: 0.94684
Ran for 253050 frames Cell Events: 8323 mean_circ: 0.946855
Ran for 253550 frames Cell Events: 8338 mean_circ: 0.946851
Ran for 254050 frames Cell Events: 8356 mean_circ: 0.946873
Ran for 254550 frames Cell Events: 8374 mean_circ: 0.946899
Ran for 255050 frames Cell Events: 8386 mean_circ: 0.946911
Ran for 255550 frames Cell Events: 8400 mean_circ: 0.946924
Ran for 256550 frames Cell Events: 8433 mean_circ: 0.946953
Ran for 257050 frames Cell Events: 8442 mean_circ: 0.946968
Ran for 257550 frames Cell Events: 8458 mean_circ: 0.946987
Ran for 258050 frames Cell Events: 8476 mean_circ: 0.946997
Ran for 258550 frames Cell Events: 8492 mean_circ: 0.946978
Ran for 259050 frames Cell Events: 8511 mean_circ: 0.94698
Ran for 259550 frames Cell Events: 8528 mean_circ: 0.946977
Ran for 260050 frames Cell Events: 8547 mean_circ: 0.946953
Ran for 260550 frames Cell Events: 8567 mean_circ: 0.946977
Ran for 261050 frames Cell Events: 8588 mean_circ: 0.946983
Ran for 261550 frames Cell Events: 8601 mean_circ: 0.947001
Ran for 262050 frames Cell Events: 8615 mean_circ: 0.946967
Ran for 263050 frames Cell Events: 8652 mean_circ: 0.946949
Ran for 264050 frames Cell Events: 8684 mean_circ: 0.946958
Ran for 264550 frames Cell Events: 8692 mean_circ: 0.946937
Ran for 265050 frames Cell Events: 8706 mean_circ: 0.946932
Ran for 265550 frames Cell Events: 8719 mean_circ: 0.946937
Ran for 266050 frames Cell Events: 8738 mean_circ: 0.946932
Ran for 266550 frames Cell Events: 8761 mean_circ: 0.946908
Ran for 267050 frames Cell Events: 8784 mean_circ: 0.946893
Ran for 267550 frames Cell Events: 8799 mean_circ: 0.94687
Ran for 268050 frames Cell Events: 8813 mean_circ: 0.946858
Ran for 270050 frames Cell Events: 8905 mean_circ: 0.946885
Ran for 270550 frames Cell Events: 8928 mean_circ: 0.946855
Ran for 271050 frames Cell Events: 8946 mean_circ: 0.94687
Ran for 272550 frames Cell Events: 8992 mean_circ: 0.946806
Ran for 274050 frames Cell Events: 9025 mean_circ: 0.946769
Ran for 274550 frames Cell Events: 9052 mean_circ: 0.946752
Ran for 275050 frames Cell Events: 9067 mean_circ: 0.946754
End this measurment..
Stopping time: 3.908e+008
****************************************
Measurement finished
Mean Circularitiy: 0.946746
Mean Area: 630.856
Mean FL-1 intensity: 2545.71
Mean FL-2 intensity: 3122.11
Mean FL-3 intensity: 0
frames processed: 275642
Total Time: 137987ms
Number of Runs (new bg img) 0
Frames collected: 275815
Skipped frames: 15316
Rescued Frames: 2286381
Samples DAQ: 68953934
Samples written: 137907236
Samples per Channel: 68953618
FPS: 1998.85
Samples per second: 499712
Expected samples/s: 500000
Events: 9095
Events per second: 65.9121
jumped back (PMT): 6311
#Frames read, frequency
# 0 frames :387057
# 1 frames :143776
# 2 frames :6381
# 3 frames :2098
# 4 frames :1318
# 5 frames :553
# 6 frames :1320
# 7 frames :2541
# 8 frames :1161
# 9 frames :797
# 10 frames :1085
# 11 frames :717
# 12 frames :598
# 13 frames :473
# 14 frames :291
# 15 frames :282
# 16 frames :246
# 17 frames :224
# 18 frames :151
# 19 frames :114
# 20 frames :73
# 21 frames :45
# 22 frames :55
# 23 frames :33
# 24 frames :25
# 25 frames :29
# 26 frames :14
# 27 frames :26
# 28 frames :15
# 29 frames :10
# 30 frames :11
# 31 frames :9
# 32 frames :10
# 33 frames :3
# 34 frames :3
# 35 frames :2
# 36 frames :1
# 37 frames :3
# 38 frames :3
# 39 frames :1
# 40 frames :4
# 41 frames :3
# 42 frames :1
# 43 frames :1
# 44 frames :4
# 45 frames :1
# 47 frames :1
# 52 frames :1
# 56 frames :1
# 65 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.924
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0