Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_18.50.17
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "a5ed8d40dd921119f8e922df92889d53",
"name": "M1_20170618_1629_dsRNA_Q6_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "16:50:38"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8453 (max 8454) in '8453'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0475
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.93489e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 20 mean_circ: 0.954963
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Ran for 199050 frames Cell Events: 7404 mean_circ: 0.954201
Ran for 200050 frames Cell Events: 7440 mean_circ: 0.954217
Ran for 200550 frames Cell Events: 7460 mean_circ: 0.954199
Ran for 201050 frames Cell Events: 7488 mean_circ: 0.954208
Ran for 202050 frames Cell Events: 7538 mean_circ: 0.954237
Ran for 203550 frames Cell Events: 7598 mean_circ: 0.954233
Ran for 204550 frames Cell Events: 7637 mean_circ: 0.954267
Ran for 205050 frames Cell Events: 7658 mean_circ: 0.95429
Ran for 205550 frames Cell Events: 7682 mean_circ: 0.954287
Ran for 206050 frames Cell Events: 7699 mean_circ: 0.954292
Ran for 207050 frames Cell Events: 7760 mean_circ: 0.95429
Ran for 207550 frames Cell Events: 7778 mean_circ: 0.954302
Ran for 208550 frames Cell Events: 7826 mean_circ: 0.954289
Ran for 209550 frames Cell Events: 7877 mean_circ: 0.954271
Ran for 210050 frames Cell Events: 7904 mean_circ: 0.95432
Ran for 210550 frames Cell Events: 7926 mean_circ: 0.954295
Ran for 212050 frames Cell Events: 7985 mean_circ: 0.954221
Ran for 212550 frames Cell Events: 8008 mean_circ: 0.954226
Ran for 215550 frames Cell Events: 8182 mean_circ: 0.954267
Ran for 216050 frames Cell Events: 8201 mean_circ: 0.954292
Ran for 216550 frames Cell Events: 8223 mean_circ: 0.954304
Ran for 217550 frames Cell Events: 8258 mean_circ: 0.9543
Ran for 218050 frames Cell Events: 8285 mean_circ: 0.954307
Ran for 218550 frames Cell Events: 8308 mean_circ: 0.954325
Ran for 219050 frames Cell Events: 8331 mean_circ: 0.954342
Ran for 219550 frames Cell Events: 8356 mean_circ: 0.954317
End this measurment..
Stopping time: 3.936e+008
****************************************
Measurement finished
Mean Circularitiy: 0.954356
Mean Area: 576.548
Mean FL-1 intensity: 3030.87
Mean FL-2 intensity: 3540.8
Mean FL-3 intensity: 0
frames processed: 221809
Total Time: 111033ms
Number of Runs (new bg img) 0
Frames collected: 221921
Skipped frames: 18385
Rescued Frames: 3041036
Samples DAQ: 55480312
Samples written: 110960542
Samples per Channel: 55480271
FPS: 1998.7
Samples per second: 499675
Expected samples/s: 500000
Events: 8454
Events per second: 76.1397
jumped back (PMT): 6173
#Frames read, frequency
# 0 frames :313806
# 1 frames :111952
# 2 frames :5790
# 3 frames :1655
# 4 frames :1031
# 5 frames :349
# 6 frames :1210
# 7 frames :2677
# 8 frames :986
# 9 frames :708
# 10 frames :1417
# 11 frames :503
# 12 frames :337
# 13 frames :282
# 14 frames :196
# 15 frames :200
# 16 frames :186
# 17 frames :132
# 18 frames :92
# 19 frames :56
# 20 frames :36
# 21 frames :42
# 22 frames :26
# 23 frames :33
# 24 frames :23
# 25 frames :19
# 26 frames :11
# 27 frames :9
# 28 frames :8
# 29 frames :9
# 30 frames :7
# 31 frames :3
# 32 frames :8
# 33 frames :3
# 35 frames :1
# 36 frames :2
# 37 frames :1
# 38 frames :1
# 42 frames :2
# 43 frames :1
# 48 frames :2
# 50 frames :1
# 54 frames :1
# 58 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0475
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0