Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_18.57.35
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "dfdaccbfcf468c27e48f001a90c65d35",
"name": "M1_20170618_1809_dsRNA_S2_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "16:58:00"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9536 (max 9537) in '9536'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0149
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.99534e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 20 mean_circ: 0.931965
Ran for 1050 frames Cell Events: 36 mean_circ: 0.931989
Ran for 1550 frames Cell Events: 48 mean_circ: 0.935073
Ran for 2550 frames Cell Events: 83 mean_circ: 0.935828
Ran for 3050 frames Cell Events: 106 mean_circ: 0.938693
Ran for 3550 frames Cell Events: 119 mean_circ: 0.939727
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Ran for 6050 frames Cell Events: 192 mean_circ: 0.941433
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Ran for 73550 frames Cell Events: 2446 mean_circ: 0.940326
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Ran for 83050 frames Cell Events: 2768 mean_circ: 0.940328
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Ran for 85550 frames Cell Events: 2863 mean_circ: 0.940414
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Ran for 86550 frames Cell Events: 2900 mean_circ: 0.94031
Ran for 87050 frames Cell Events: 2912 mean_circ: 0.940297
Ran for 87550 frames Cell Events: 2928 mean_circ: 0.940302
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Ran for 89550 frames Cell Events: 2997 mean_circ: 0.940321
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Ran for 91550 frames Cell Events: 3070 mean_circ: 0.940284
Ran for 92550 frames Cell Events: 3106 mean_circ: 0.940252
Ran for 93050 frames Cell Events: 3126 mean_circ: 0.940283
Ran for 94050 frames Cell Events: 3164 mean_circ: 0.940255
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Ran for 97550 frames Cell Events: 3292 mean_circ: 0.940149
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Ran for 100050 frames Cell Events: 3384 mean_circ: 0.940165
Ran for 102050 frames Cell Events: 3474 mean_circ: 0.940253
Ran for 103550 frames Cell Events: 3536 mean_circ: 0.940276
Ran for 104550 frames Cell Events: 3573 mean_circ: 0.940146
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Ran for 122550 frames Cell Events: 4238 mean_circ: 0.940142
Ran for 123050 frames Cell Events: 4259 mean_circ: 0.940161
Ran for 123550 frames Cell Events: 4275 mean_circ: 0.940215
Ran for 124050 frames Cell Events: 4289 mean_circ: 0.940215
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Ran for 125050 frames Cell Events: 4314 mean_circ: 0.940189
Ran for 125550 frames Cell Events: 4327 mean_circ: 0.94019
Ran for 126550 frames Cell Events: 4366 mean_circ: 0.940199
Ran for 127550 frames Cell Events: 4417 mean_circ: 0.940218
Ran for 128050 frames Cell Events: 4431 mean_circ: 0.940196
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Ran for 131550 frames Cell Events: 4558 mean_circ: 0.940056
Ran for 132050 frames Cell Events: 4569 mean_circ: 0.940025
Ran for 133050 frames Cell Events: 4607 mean_circ: 0.940095
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Ran for 142050 frames Cell Events: 4904 mean_circ: 0.939993
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Ran for 188550 frames Cell Events: 6514 mean_circ: 0.939987
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Ran for 189550 frames Cell Events: 6550 mean_circ: 0.939953
Ran for 190050 frames Cell Events: 6562 mean_circ: 0.939965
Ran for 191050 frames Cell Events: 6597 mean_circ: 0.940005
Ran for 191550 frames Cell Events: 6612 mean_circ: 0.940002
Ran for 192050 frames Cell Events: 6631 mean_circ: 0.939993
Ran for 192550 frames Cell Events: 6648 mean_circ: 0.94001
Ran for 193050 frames Cell Events: 6662 mean_circ: 0.93998
Ran for 193550 frames Cell Events: 6676 mean_circ: 0.939996
Ran for 194050 frames Cell Events: 6691 mean_circ: 0.939923
Ran for 195050 frames Cell Events: 6720 mean_circ: 0.939891
Ran for 195550 frames Cell Events: 6735 mean_circ: 0.939881
Ran for 196050 frames Cell Events: 6755 mean_circ: 0.939854
Ran for 196550 frames Cell Events: 6775 mean_circ: 0.939889
Ran for 197050 frames Cell Events: 6791 mean_circ: 0.939882
Ran for 198050 frames Cell Events: 6824 mean_circ: 0.939903
Ran for 198550 frames Cell Events: 6838 mean_circ: 0.939886
Ran for 199050 frames Cell Events: 6852 mean_circ: 0.939881
Ran for 200050 frames Cell Events: 6879 mean_circ: 0.939911
Ran for 201050 frames Cell Events: 6920 mean_circ: 0.939944
Ran for 201550 frames Cell Events: 6934 mean_circ: 0.939942
Ran for 202550 frames Cell Events: 6982 mean_circ: 0.939898
Ran for 203550 frames Cell Events: 7012 mean_circ: 0.939871
Ran for 204050 frames Cell Events: 7031 mean_circ: 0.939893
Ran for 204550 frames Cell Events: 7047 mean_circ: 0.939867
Ran for 205050 frames Cell Events: 7063 mean_circ: 0.939903
Ran for 205550 frames Cell Events: 7081 mean_circ: 0.93988
Ran for 206050 frames Cell Events: 7101 mean_circ: 0.939901
Ran for 207050 frames Cell Events: 7144 mean_circ: 0.939891
Ran for 208550 frames Cell Events: 7204 mean_circ: 0.939936
Ran for 209050 frames Cell Events: 7221 mean_circ: 0.939955
Ran for 209550 frames Cell Events: 7247 mean_circ: 0.939984
Ran for 210050 frames Cell Events: 7266 mean_circ: 0.940011
Ran for 210550 frames Cell Events: 7281 mean_circ: 0.940031
Ran for 211050 frames Cell Events: 7301 mean_circ: 0.940032
Ran for 211550 frames Cell Events: 7316 mean_circ: 0.940018
Ran for 212550 frames Cell Events: 7347 mean_circ: 0.940055
Ran for 214050 frames Cell Events: 7409 mean_circ: 0.940071
Ran for 214550 frames Cell Events: 7418 mean_circ: 0.940067
Ran for 216050 frames Cell Events: 7475 mean_circ: 0.939979
Ran for 216550 frames Cell Events: 7500 mean_circ: 0.93994
Ran for 217050 frames Cell Events: 7509 mean_circ: 0.939944
Ran for 217550 frames Cell Events: 7529 mean_circ: 0.939915
Ran for 218050 frames Cell Events: 7546 mean_circ: 0.939889
Ran for 218550 frames Cell Events: 7565 mean_circ: 0.93991
Ran for 219050 frames Cell Events: 7578 mean_circ: 0.939885
Ran for 219550 frames Cell Events: 7589 mean_circ: 0.939884
Ran for 221050 frames Cell Events: 7634 mean_circ: 0.939909
Ran for 222050 frames Cell Events: 7663 mean_circ: 0.93992
Ran for 223050 frames Cell Events: 7698 mean_circ: 0.939942
Ran for 223550 frames Cell Events: 7723 mean_circ: 0.939949
Ran for 224050 frames Cell Events: 7741 mean_circ: 0.939967
Ran for 224550 frames Cell Events: 7759 mean_circ: 0.939945
Ran for 226050 frames Cell Events: 7821 mean_circ: 0.939933
Ran for 228050 frames Cell Events: 7914 mean_circ: 0.939917
Ran for 230050 frames Cell Events: 7988 mean_circ: 0.939879
Ran for 231050 frames Cell Events: 8026 mean_circ: 0.93989
Ran for 231550 frames Cell Events: 8042 mean_circ: 0.939913
Ran for 232050 frames Cell Events: 8061 mean_circ: 0.939894
Ran for 233550 frames Cell Events: 8108 mean_circ: 0.939892
Ran for 234050 frames Cell Events: 8125 mean_circ: 0.939882
Ran for 234550 frames Cell Events: 8148 mean_circ: 0.939887
Ran for 235050 frames Cell Events: 8177 mean_circ: 0.939877
Ran for 235550 frames Cell Events: 8196 mean_circ: 0.939861
Ran for 236050 frames Cell Events: 8214 mean_circ: 0.939823
Ran for 237550 frames Cell Events: 8264 mean_circ: 0.93984
Ran for 238550 frames Cell Events: 8298 mean_circ: 0.939811
Ran for 240050 frames Cell Events: 8357 mean_circ: 0.939801
Ran for 241550 frames Cell Events: 8408 mean_circ: 0.939799
Ran for 242550 frames Cell Events: 8454 mean_circ: 0.939819
Ran for 243050 frames Cell Events: 8476 mean_circ: 0.939835
Ran for 244050 frames Cell Events: 8505 mean_circ: 0.93978
Ran for 244550 frames Cell Events: 8525 mean_circ: 0.939765
Ran for 245050 frames Cell Events: 8551 mean_circ: 0.939761
Ran for 246050 frames Cell Events: 8585 mean_circ: 0.939676
Ran for 246550 frames Cell Events: 8598 mean_circ: 0.939637
Ran for 247050 frames Cell Events: 8606 mean_circ: 0.939639
Ran for 248550 frames Cell Events: 8668 mean_circ: 0.939629
Ran for 249550 frames Cell Events: 8709 mean_circ: 0.93961
Ran for 250550 frames Cell Events: 8762 mean_circ: 0.939467
Ran for 251050 frames Cell Events: 8785 mean_circ: 0.939473
Ran for 252050 frames Cell Events: 8811 mean_circ: 0.939495
Ran for 252550 frames Cell Events: 8831 mean_circ: 0.939473
Ran for 253050 frames Cell Events: 8848 mean_circ: 0.939466
Ran for 253550 frames Cell Events: 8859 mean_circ: 0.939455
Ran for 254050 frames Cell Events: 8876 mean_circ: 0.93946
Ran for 254550 frames Cell Events: 8898 mean_circ: 0.939471
Ran for 255050 frames Cell Events: 8913 mean_circ: 0.93948
Ran for 255550 frames Cell Events: 8926 mean_circ: 0.939442
Ran for 257050 frames Cell Events: 8966 mean_circ: 0.939449
Ran for 257550 frames Cell Events: 8983 mean_circ: 0.939405
Ran for 258550 frames Cell Events: 9011 mean_circ: 0.939325
Ran for 259550 frames Cell Events: 9045 mean_circ: 0.939307
Ran for 260050 frames Cell Events: 9062 mean_circ: 0.939306
Ran for 260550 frames Cell Events: 9077 mean_circ: 0.939268
Ran for 261050 frames Cell Events: 9090 mean_circ: 0.939247
Ran for 261550 frames Cell Events: 9105 mean_circ: 0.939241
Ran for 262050 frames Cell Events: 9117 mean_circ: 0.939232
Ran for 263050 frames Cell Events: 9146 mean_circ: 0.939239
Ran for 263550 frames Cell Events: 9160 mean_circ: 0.939214
Ran for 264550 frames Cell Events: 9196 mean_circ: 0.93917
Ran for 265050 frames Cell Events: 9211 mean_circ: 0.939153
Ran for 266050 frames Cell Events: 9236 mean_circ: 0.939169
Ran for 266550 frames Cell Events: 9257 mean_circ: 0.93918
Ran for 267550 frames Cell Events: 9304 mean_circ: 0.939181
Ran for 268550 frames Cell Events: 9345 mean_circ: 0.93922
Ran for 270050 frames Cell Events: 9395 mean_circ: 0.939248
Ran for 270550 frames Cell Events: 9414 mean_circ: 0.939248
Ran for 271050 frames Cell Events: 9436 mean_circ: 0.939251
Ran for 271550 frames Cell Events: 9460 mean_circ: 0.93922
Ran for 272050 frames Cell Events: 9474 mean_circ: 0.939217
Ran for 273050 frames Cell Events: 9507 mean_circ: 0.939174
Ran for 273550 frames Cell Events: 9521 mean_circ: 0.939156
End this measurment..
Stopping time: 3.99671e+008
****************************************
Measurement finished
Mean Circularitiy: 0.939149
Mean Area: 556.112
Mean FL-1 intensity: 2732.01
Mean FL-2 intensity: 3699.09
Mean FL-3 intensity: 0
frames processed: 272450
Total Time: 137033ms
Number of Runs (new bg img) 0
Frames collected: 273959
Skipped frames: 3701
Rescued Frames: 598844
Samples DAQ: 68489874
Samples written: 136979536
Samples per Channel: 68489768
FPS: 1999.22
Samples per second: 499805
Expected samples/s: 500000
Events: 9537
Events per second: 69.5964
jumped back (PMT): 11
#Frames read, frequency
# 0 frames :376308
# 1 frames :150743
# 2 frames :6681
# 3 frames :1839
# 4 frames :987
# 5 frames :374
# 6 frames :2893
# 7 frames :2461
# 8 frames :1976
# 9 frames :872
# 10 frames :476
# 11 frames :380
# 12 frames :462
# 13 frames :274
# 14 frames :276
# 15 frames :211
# 16 frames :167
# 17 frames :124
# 18 frames :90
# 19 frames :61
# 20 frames :51
# 21 frames :39
# 22 frames :35
# 23 frames :19
# 24 frames :18
# 25 frames :11
# 26 frames :11
# 27 frames :8
# 28 frames :10
# 29 frames :7
# 30 frames :9
# 31 frames :7
# 32 frames :2
# 33 frames :3
# 34 frames :4
# 35 frames :1
# 36 frames :4
# 37 frames :1
# 39 frames :1
# 40 frames :2
# 41 frames :2
# 47 frames :1
# 48 frames :1
# 49 frames :1
# 50 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0149
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0