Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_19.09.05
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "aa0d2f6ec5ba18f8758fd113896c63b4",
"name": "M1_20170619_1342_dsRNA_V7_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:09:26"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8522 (max 8523) in '8522'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9711
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 4.69892e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 46 mean_circ: 0.943207
Ran for 2050 frames Cell Events: 99 mean_circ: 0.942392
Ran for 2550 frames Cell Events: 121 mean_circ: 0.944773
Ran for 3550 frames Cell Events: 158 mean_circ: 0.946731
Ran for 4550 frames Cell Events: 204 mean_circ: 0.948635
Ran for 5050 frames Cell Events: 220 mean_circ: 0.948475
Ran for 5550 frames Cell Events: 239 mean_circ: 0.948361
Ran for 6550 frames Cell Events: 285 mean_circ: 0.947739
Ran for 7550 frames Cell Events: 321 mean_circ: 0.947932
Ran for 8050 frames Cell Events: 334 mean_circ: 0.947632
Ran for 8550 frames Cell Events: 354 mean_circ: 0.94655
Ran for 9050 frames Cell Events: 373 mean_circ: 0.946619
Ran for 9550 frames Cell Events: 387 mean_circ: 0.946141
Ran for 10050 frames Cell Events: 414 mean_circ: 0.946235
Ran for 10550 frames Cell Events: 430 mean_circ: 0.946745
Ran for 11050 frames Cell Events: 442 mean_circ: 0.946627
Ran for 11550 frames Cell Events: 472 mean_circ: 0.946381
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Ran for 12550 frames Cell Events: 515 mean_circ: 0.947053
Ran for 13050 frames Cell Events: 537 mean_circ: 0.94758
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Ran for 14050 frames Cell Events: 577 mean_circ: 0.947
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Ran for 77050 frames Cell Events: 2966 mean_circ: 0.94788
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Ran for 78050 frames Cell Events: 3000 mean_circ: 0.947966
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Ran for 82050 frames Cell Events: 3170 mean_circ: 0.948268
Ran for 83550 frames Cell Events: 3226 mean_circ: 0.948365
Ran for 84050 frames Cell Events: 3252 mean_circ: 0.948347
Ran for 84550 frames Cell Events: 3260 mean_circ: 0.948353
Ran for 85050 frames Cell Events: 3278 mean_circ: 0.948378
Ran for 85550 frames Cell Events: 3291 mean_circ: 0.948326
Ran for 86550 frames Cell Events: 3327 mean_circ: 0.948317
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Ran for 92050 frames Cell Events: 3526 mean_circ: 0.948353
Ran for 92550 frames Cell Events: 3546 mean_circ: 0.948363
Ran for 93550 frames Cell Events: 3590 mean_circ: 0.948301
Ran for 94050 frames Cell Events: 3606 mean_circ: 0.948332
Ran for 94550 frames Cell Events: 3620 mean_circ: 0.948401
Ran for 95050 frames Cell Events: 3639 mean_circ: 0.948429
Ran for 96050 frames Cell Events: 3688 mean_circ: 0.94824
Ran for 98050 frames Cell Events: 3767 mean_circ: 0.948277
Ran for 98550 frames Cell Events: 3779 mean_circ: 0.948316
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Ran for 100050 frames Cell Events: 3836 mean_circ: 0.948356
Ran for 100550 frames Cell Events: 3847 mean_circ: 0.948307
Ran for 101050 frames Cell Events: 3857 mean_circ: 0.948325
Ran for 102050 frames Cell Events: 3884 mean_circ: 0.948301
Ran for 102550 frames Cell Events: 3905 mean_circ: 0.948217
Ran for 103050 frames Cell Events: 3924 mean_circ: 0.94827
Ran for 103550 frames Cell Events: 3945 mean_circ: 0.948276
Ran for 104050 frames Cell Events: 3962 mean_circ: 0.948283
Ran for 104550 frames Cell Events: 3974 mean_circ: 0.948286
Ran for 105050 frames Cell Events: 3998 mean_circ: 0.948296
Ran for 107050 frames Cell Events: 4063 mean_circ: 0.948289
Ran for 108550 frames Cell Events: 4117 mean_circ: 0.948277
Ran for 110550 frames Cell Events: 4208 mean_circ: 0.948169
Ran for 111050 frames Cell Events: 4238 mean_circ: 0.948217
Ran for 111550 frames Cell Events: 4259 mean_circ: 0.94823
Ran for 112550 frames Cell Events: 4293 mean_circ: 0.948126
Ran for 113050 frames Cell Events: 4309 mean_circ: 0.948159
Ran for 113550 frames Cell Events: 4328 mean_circ: 0.948093
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Ran for 114550 frames Cell Events: 4361 mean_circ: 0.948127
Ran for 116050 frames Cell Events: 4434 mean_circ: 0.948063
Ran for 117050 frames Cell Events: 4469 mean_circ: 0.948044
Ran for 117550 frames Cell Events: 4490 mean_circ: 0.948092
Ran for 118550 frames Cell Events: 4528 mean_circ: 0.948111
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Ran for 121050 frames Cell Events: 4627 mean_circ: 0.948143
Ran for 121550 frames Cell Events: 4657 mean_circ: 0.948137
Ran for 122550 frames Cell Events: 4727 mean_circ: 0.948121
Ran for 124050 frames Cell Events: 4787 mean_circ: 0.948006
Ran for 124550 frames Cell Events: 4802 mean_circ: 0.948023
Ran for 125550 frames Cell Events: 4854 mean_circ: 0.947987
Ran for 126050 frames Cell Events: 4874 mean_circ: 0.948022
Ran for 126550 frames Cell Events: 4894 mean_circ: 0.94802
Ran for 127050 frames Cell Events: 4916 mean_circ: 0.947971
Ran for 128550 frames Cell Events: 4984 mean_circ: 0.947839
Ran for 129050 frames Cell Events: 5002 mean_circ: 0.947818
Ran for 131050 frames Cell Events: 5083 mean_circ: 0.947875
Ran for 132050 frames Cell Events: 5120 mean_circ: 0.947948
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Ran for 136050 frames Cell Events: 5336 mean_circ: 0.947833
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Ran for 137550 frames Cell Events: 5412 mean_circ: 0.947854
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Ran for 139050 frames Cell Events: 5468 mean_circ: 0.947783
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Ran for 141050 frames Cell Events: 5552 mean_circ: 0.947867
Ran for 144550 frames Cell Events: 5719 mean_circ: 0.947932
Ran for 147050 frames Cell Events: 5816 mean_circ: 0.947921
Ran for 147550 frames Cell Events: 5828 mean_circ: 0.947947
Ran for 149050 frames Cell Events: 5909 mean_circ: 0.947892
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Ran for 150050 frames Cell Events: 5949 mean_circ: 0.947898
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Ran for 152550 frames Cell Events: 6088 mean_circ: 0.947782
Ran for 153050 frames Cell Events: 6106 mean_circ: 0.947777
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Ran for 154550 frames Cell Events: 6165 mean_circ: 0.947702
Ran for 156050 frames Cell Events: 6235 mean_circ: 0.947692
Ran for 156550 frames Cell Events: 6256 mean_circ: 0.947728
Ran for 157550 frames Cell Events: 6288 mean_circ: 0.947756
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Ran for 181550 frames Cell Events: 7344 mean_circ: 0.947411
Ran for 182050 frames Cell Events: 7369 mean_circ: 0.947402
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Ran for 200550 frames Cell Events: 8114 mean_circ: 0.947382
Ran for 201050 frames Cell Events: 8140 mean_circ: 0.947377
Ran for 201550 frames Cell Events: 8161 mean_circ: 0.947383
Ran for 202050 frames Cell Events: 8187 mean_circ: 0.947384
Ran for 206050 frames Cell Events: 8376 mean_circ: 0.947253
Ran for 206550 frames Cell Events: 8395 mean_circ: 0.947263
Ran for 207050 frames Cell Events: 8416 mean_circ: 0.947273
Ran for 207550 frames Cell Events: 8443 mean_circ: 0.94727
Ran for 208050 frames Cell Events: 8462 mean_circ: 0.947272
Ran for 208550 frames Cell Events: 8494 mean_circ: 0.947303
End this measurment..
Stopping time: 4.69996e+008
****************************************
Measurement finished
Mean Circularitiy: 0.947286
Mean Area: 580.064
Mean FL-1 intensity: 3373.86
Mean FL-2 intensity: 3977.54
Mean FL-3 intensity: 0
frames processed: 209017
Total Time: 104613ms
Number of Runs (new bg img) 0
Frames collected: 209070
Skipped frames: 2610
Rescued Frames: 452135
Samples DAQ: 52268049
Samples written: 104534928
Samples per Channel: 52267464
FPS: 1998.51
Samples per second: 499628
Expected samples/s: 500000
Events: 8523
Events per second: 81.4718
jumped back (PMT): 5540
#Frames read, frequency
# 0 frames :301030
# 1 frames :99557
# 2 frames :5270
# 3 frames :1665
# 4 frames :972
# 5 frames :430
# 6 frames :1290
# 7 frames :2422
# 8 frames :993
# 9 frames :633
# 10 frames :982
# 11 frames :720
# 12 frames :501
# 13 frames :334
# 14 frames :192
# 15 frames :211
# 16 frames :171
# 17 frames :130
# 18 frames :86
# 19 frames :61
# 20 frames :45
# 21 frames :62
# 22 frames :35
# 23 frames :27
# 24 frames :32
# 25 frames :25
# 26 frames :19
# 27 frames :13
# 28 frames :6
# 29 frames :8
# 30 frames :6
# 31 frames :5
# 32 frames :4
# 33 frames :7
# 34 frames :3
# 35 frames :3
# 36 frames :1
# 37 frames :4
# 38 frames :1
# 40 frames :2
# 41 frames :2
# 42 frames :5
# 44 frames :2
# 46 frames :3
# 53 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9711
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0