Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_19.15.55
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "4f8192b997b188c29879f8728898715c",
"name": "M1_20170619_1508_dsRNA_V8_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:16:16"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8112 (max 8113) in '8112'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0804
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 4.75038e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 27 mean_circ: 0.958835
Ran for 2050 frames Cell Events: 54 mean_circ: 0.957515
Ran for 2550 frames Cell Events: 78 mean_circ: 0.957378
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Ran for 5050 frames Cell Events: 167 mean_circ: 0.956723
Ran for 5550 frames Cell Events: 180 mean_circ: 0.956457
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Ran for 6550 frames Cell Events: 220 mean_circ: 0.957186
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Ran for 58050 frames Cell Events: 1889 mean_circ: 0.956169
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Ran for 59550 frames Cell Events: 1946 mean_circ: 0.956211
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Ran for 72550 frames Cell Events: 2384 mean_circ: 0.956633
Ran for 73050 frames Cell Events: 2401 mean_circ: 0.956561
Ran for 73550 frames Cell Events: 2420 mean_circ: 0.956642
Ran for 74550 frames Cell Events: 2462 mean_circ: 0.956775
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Ran for 76050 frames Cell Events: 2516 mean_circ: 0.956732
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Ran for 77550 frames Cell Events: 2576 mean_circ: 0.956665
Ran for 78050 frames Cell Events: 2588 mean_circ: 0.956612
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Ran for 80050 frames Cell Events: 2668 mean_circ: 0.956728
Ran for 80550 frames Cell Events: 2695 mean_circ: 0.956658
Ran for 81050 frames Cell Events: 2716 mean_circ: 0.956639
Ran for 82050 frames Cell Events: 2779 mean_circ: 0.956688
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Ran for 84050 frames Cell Events: 2856 mean_circ: 0.956655
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Ran for 87050 frames Cell Events: 2974 mean_circ: 0.95672
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Ran for 92050 frames Cell Events: 3179 mean_circ: 0.956914
Ran for 93550 frames Cell Events: 3237 mean_circ: 0.957024
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Ran for 102050 frames Cell Events: 3564 mean_circ: 0.957058
Ran for 102550 frames Cell Events: 3600 mean_circ: 0.957046
Ran for 103050 frames Cell Events: 3627 mean_circ: 0.957104
Ran for 104550 frames Cell Events: 3688 mean_circ: 0.957029
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Ran for 106050 frames Cell Events: 3767 mean_circ: 0.95709
Ran for 106550 frames Cell Events: 3795 mean_circ: 0.957125
Ran for 108050 frames Cell Events: 3855 mean_circ: 0.957179
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Ran for 109550 frames Cell Events: 3918 mean_circ: 0.95716
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Ran for 111050 frames Cell Events: 3968 mean_circ: 0.957124
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Ran for 115050 frames Cell Events: 4119 mean_circ: 0.957166
Ran for 115550 frames Cell Events: 4158 mean_circ: 0.957078
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Ran for 121550 frames Cell Events: 4374 mean_circ: 0.957123
Ran for 122050 frames Cell Events: 4396 mean_circ: 0.957146
Ran for 122550 frames Cell Events: 4414 mean_circ: 0.957219
Ran for 123050 frames Cell Events: 4440 mean_circ: 0.957193
Ran for 123550 frames Cell Events: 4457 mean_circ: 0.957203
Ran for 124050 frames Cell Events: 4473 mean_circ: 0.95726
Ran for 124550 frames Cell Events: 4494 mean_circ: 0.957235
Ran for 125050 frames Cell Events: 4513 mean_circ: 0.957267
Ran for 125550 frames Cell Events: 4523 mean_circ: 0.957263
Ran for 126050 frames Cell Events: 4547 mean_circ: 0.957276
Ran for 127050 frames Cell Events: 4583 mean_circ: 0.957247
Ran for 127550 frames Cell Events: 4600 mean_circ: 0.957202
Ran for 129050 frames Cell Events: 4649 mean_circ: 0.957156
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Ran for 131050 frames Cell Events: 4705 mean_circ: 0.957237
Ran for 131550 frames Cell Events: 4729 mean_circ: 0.957248
Ran for 132050 frames Cell Events: 4739 mean_circ: 0.957229
Ran for 132550 frames Cell Events: 4750 mean_circ: 0.957235
Ran for 133050 frames Cell Events: 4764 mean_circ: 0.957257
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Ran for 152550 frames Cell Events: 5478 mean_circ: 0.957433
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Ran for 163550 frames Cell Events: 5842 mean_circ: 0.957493
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Ran for 166050 frames Cell Events: 5941 mean_circ: 0.95754
Ran for 167050 frames Cell Events: 5987 mean_circ: 0.957489
Ran for 167550 frames Cell Events: 6013 mean_circ: 0.957468
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Ran for 171050 frames Cell Events: 6138 mean_circ: 0.957384
Ran for 171550 frames Cell Events: 6150 mean_circ: 0.957389
Ran for 173050 frames Cell Events: 6214 mean_circ: 0.957438
Ran for 174050 frames Cell Events: 6246 mean_circ: 0.957465
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Ran for 177050 frames Cell Events: 6361 mean_circ: 0.957513
Ran for 177550 frames Cell Events: 6386 mean_circ: 0.957469
Ran for 178050 frames Cell Events: 6400 mean_circ: 0.957476
Ran for 179050 frames Cell Events: 6436 mean_circ: 0.957468
Ran for 180050 frames Cell Events: 6478 mean_circ: 0.957481
Ran for 180550 frames Cell Events: 6498 mean_circ: 0.957493
Ran for 181550 frames Cell Events: 6523 mean_circ: 0.9575
Ran for 182050 frames Cell Events: 6537 mean_circ: 0.957496
Ran for 183050 frames Cell Events: 6572 mean_circ: 0.957521
Ran for 183550 frames Cell Events: 6585 mean_circ: 0.957531
Ran for 184050 frames Cell Events: 6601 mean_circ: 0.95754
Ran for 184550 frames Cell Events: 6622 mean_circ: 0.957566
Ran for 185050 frames Cell Events: 6637 mean_circ: 0.957593
Ran for 185550 frames Cell Events: 6654 mean_circ: 0.957596
Ran for 186050 frames Cell Events: 6672 mean_circ: 0.957574
Ran for 186550 frames Cell Events: 6691 mean_circ: 0.957578
Ran for 188050 frames Cell Events: 6737 mean_circ: 0.957601
Ran for 188550 frames Cell Events: 6752 mean_circ: 0.957612
Ran for 189050 frames Cell Events: 6771 mean_circ: 0.957612
Ran for 189550 frames Cell Events: 6791 mean_circ: 0.957607
Ran for 190550 frames Cell Events: 6829 mean_circ: 0.957608
Ran for 191050 frames Cell Events: 6843 mean_circ: 0.957594
Ran for 191550 frames Cell Events: 6861 mean_circ: 0.9576
Ran for 192050 frames Cell Events: 6880 mean_circ: 0.957576
Ran for 192550 frames Cell Events: 6892 mean_circ: 0.957574
Ran for 193050 frames Cell Events: 6914 mean_circ: 0.957588
Ran for 194550 frames Cell Events: 6961 mean_circ: 0.957623
Ran for 195050 frames Cell Events: 6988 mean_circ: 0.957629
Ran for 195550 frames Cell Events: 7006 mean_circ: 0.957635
Ran for 196050 frames Cell Events: 7023 mean_circ: 0.957649
Ran for 196550 frames Cell Events: 7040 mean_circ: 0.95763
Ran for 197050 frames Cell Events: 7056 mean_circ: 0.957631
Ran for 198550 frames Cell Events: 7118 mean_circ: 0.957621
Ran for 199050 frames Cell Events: 7141 mean_circ: 0.957604
Ran for 199550 frames Cell Events: 7153 mean_circ: 0.957612
Ran for 200050 frames Cell Events: 7171 mean_circ: 0.957627
Ran for 200550 frames Cell Events: 7188 mean_circ: 0.957626
Ran for 201550 frames Cell Events: 7218 mean_circ: 0.957641
Ran for 202050 frames Cell Events: 7238 mean_circ: 0.957639
Ran for 202550 frames Cell Events: 7248 mean_circ: 0.957635
Ran for 203050 frames Cell Events: 7263 mean_circ: 0.957645
Ran for 203550 frames Cell Events: 7285 mean_circ: 0.957625
Ran for 204550 frames Cell Events: 7317 mean_circ: 0.957645
Ran for 205050 frames Cell Events: 7329 mean_circ: 0.95763
Ran for 205550 frames Cell Events: 7341 mean_circ: 0.957625
Ran for 207050 frames Cell Events: 7389 mean_circ: 0.957687
Ran for 208550 frames Cell Events: 7450 mean_circ: 0.957688
Ran for 209050 frames Cell Events: 7465 mean_circ: 0.957699
Ran for 211550 frames Cell Events: 7545 mean_circ: 0.957721
Ran for 212550 frames Cell Events: 7578 mean_circ: 0.957735
Ran for 213050 frames Cell Events: 7596 mean_circ: 0.957723
Ran for 213550 frames Cell Events: 7614 mean_circ: 0.957723
Ran for 214050 frames Cell Events: 7642 mean_circ: 0.957698
Ran for 215050 frames Cell Events: 7675 mean_circ: 0.957675
Ran for 215550 frames Cell Events: 7695 mean_circ: 0.957677
Ran for 216550 frames Cell Events: 7737 mean_circ: 0.957678
Ran for 217050 frames Cell Events: 7749 mean_circ: 0.957691
Ran for 217550 frames Cell Events: 7770 mean_circ: 0.957721
Ran for 218050 frames Cell Events: 7784 mean_circ: 0.957729
Ran for 218550 frames Cell Events: 7803 mean_circ: 0.957728
Ran for 219050 frames Cell Events: 7821 mean_circ: 0.957721
Ran for 219550 frames Cell Events: 7842 mean_circ: 0.957723
Ran for 220550 frames Cell Events: 7898 mean_circ: 0.957693
Ran for 223050 frames Cell Events: 7981 mean_circ: 0.957649
Ran for 223550 frames Cell Events: 7993 mean_circ: 0.957656
Ran for 224550 frames Cell Events: 8034 mean_circ: 0.957595
Ran for 226050 frames Cell Events: 8110 mean_circ: 0.957611
End this measurment..
Stopping time: 4.75151e+008
****************************************
Measurement finished
Mean Circularitiy: 0.957618
Mean Area: 614.948
Mean FL-1 intensity: 3510.15
Mean FL-2 intensity: 4261.63
Mean FL-3 intensity: 0
frames processed: 226011
Total Time: 113180ms
Number of Runs (new bg img) 0
Frames collected: 226181
Skipped frames: 4886
Rescued Frames: 790547
Samples DAQ: 56545369
Samples written: 113090626
Samples per Channel: 56545313
FPS: 1998.41
Samples per second: 499603
Expected samples/s: 500000
Events: 8113
Events per second: 71.682
jumped back (PMT): 5521
#Frames read, frequency
# 0 frames :313358
# 1 frames :121468
# 2 frames :5027
# 3 frames :1862
# 4 frames :879
# 5 frames :1866
# 6 frames :2000
# 7 frames :1275
# 8 frames :723
# 9 frames :484
# 10 frames :1006
# 11 frames :547
# 12 frames :435
# 13 frames :287
# 14 frames :238
# 15 frames :169
# 16 frames :150
# 17 frames :118
# 18 frames :94
# 19 frames :63
# 20 frames :49
# 21 frames :43
# 22 frames :26
# 23 frames :23
# 24 frames :24
# 25 frames :17
# 26 frames :14
# 27 frames :16
# 28 frames :5
# 29 frames :12
# 30 frames :10
# 31 frames :7
# 32 frames :5
# 33 frames :5
# 34 frames :1
# 36 frames :1
# 37 frames :3
# 38 frames :2
# 39 frames :1
# 40 frames :1
# 41 frames :2
# 43 frames :2
# 44 frames :1
# 47 frames :1
# 48 frames :2
# 53 frames :1
# 54 frames :1
# 63 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0804
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0