Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_19.21.46
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "51d4e759ab008563aabc6a690e16e4b0",
"name": "M1_20170619_1622_dsRNA_U7_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:22:10"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9457 (max 9458) in '9457'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0536
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 4.7951e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 21 mean_circ: 0.948331
Ran for 1050 frames Cell Events: 34 mean_circ: 0.948593
Ran for 1550 frames Cell Events: 55 mean_circ: 0.95425
Ran for 2550 frames Cell Events: 79 mean_circ: 0.951193
Ran for 3050 frames Cell Events: 94 mean_circ: 0.952579
Ran for 3550 frames Cell Events: 116 mean_circ: 0.949935
Ran for 4050 frames Cell Events: 128 mean_circ: 0.950749
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Ran for 5050 frames Cell Events: 150 mean_circ: 0.951549
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Ran for 6550 frames Cell Events: 205 mean_circ: 0.949991
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Ran for 12550 frames Cell Events: 396 mean_circ: 0.9497
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Ran for 81050 frames Cell Events: 2991 mean_circ: 0.949676
Ran for 81550 frames Cell Events: 3014 mean_circ: 0.949698
Ran for 82550 frames Cell Events: 3063 mean_circ: 0.949806
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Ran for 83550 frames Cell Events: 3097 mean_circ: 0.949857
Ran for 84050 frames Cell Events: 3111 mean_circ: 0.949902
Ran for 84550 frames Cell Events: 3121 mean_circ: 0.949892
Ran for 85550 frames Cell Events: 3168 mean_circ: 0.949886
Ran for 86550 frames Cell Events: 3202 mean_circ: 0.949933
Ran for 87050 frames Cell Events: 3219 mean_circ: 0.949909
Ran for 88050 frames Cell Events: 3257 mean_circ: 0.949875
Ran for 89050 frames Cell Events: 3291 mean_circ: 0.949828
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Ran for 91550 frames Cell Events: 3410 mean_circ: 0.949496
Ran for 92050 frames Cell Events: 3422 mean_circ: 0.949518
Ran for 93050 frames Cell Events: 3460 mean_circ: 0.949562
Ran for 93550 frames Cell Events: 3480 mean_circ: 0.949594
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Ran for 95050 frames Cell Events: 3527 mean_circ: 0.949559
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Ran for 96050 frames Cell Events: 3567 mean_circ: 0.949579
Ran for 96550 frames Cell Events: 3586 mean_circ: 0.949561
Ran for 97050 frames Cell Events: 3610 mean_circ: 0.949642
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Ran for 102550 frames Cell Events: 3822 mean_circ: 0.949672
Ran for 103050 frames Cell Events: 3844 mean_circ: 0.949665
Ran for 103550 frames Cell Events: 3865 mean_circ: 0.949685
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Ran for 121550 frames Cell Events: 4522 mean_circ: 0.949046
Ran for 122550 frames Cell Events: 4551 mean_circ: 0.949092
Ran for 123050 frames Cell Events: 4568 mean_circ: 0.949088
Ran for 123550 frames Cell Events: 4586 mean_circ: 0.949089
Ran for 124050 frames Cell Events: 4598 mean_circ: 0.949038
Ran for 124550 frames Cell Events: 4618 mean_circ: 0.949041
Ran for 125050 frames Cell Events: 4631 mean_circ: 0.949037
Ran for 125550 frames Cell Events: 4651 mean_circ: 0.949068
Ran for 126050 frames Cell Events: 4666 mean_circ: 0.949045
Ran for 126550 frames Cell Events: 4682 mean_circ: 0.949015
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Ran for 131050 frames Cell Events: 4868 mean_circ: 0.949052
Ran for 131550 frames Cell Events: 4889 mean_circ: 0.94903
Ran for 132050 frames Cell Events: 4914 mean_circ: 0.949087
Ran for 132550 frames Cell Events: 4931 mean_circ: 0.949093
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Ran for 171550 frames Cell Events: 6517 mean_circ: 0.948921
Ran for 172050 frames Cell Events: 6546 mean_circ: 0.94892
Ran for 172550 frames Cell Events: 6568 mean_circ: 0.948911
Ran for 173050 frames Cell Events: 6588 mean_circ: 0.948913
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Ran for 182550 frames Cell Events: 6915 mean_circ: 0.948807
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Ran for 183550 frames Cell Events: 6944 mean_circ: 0.948807
Ran for 184050 frames Cell Events: 6960 mean_circ: 0.948764
Ran for 184550 frames Cell Events: 6979 mean_circ: 0.948801
Ran for 186050 frames Cell Events: 7045 mean_circ: 0.948826
Ran for 186550 frames Cell Events: 7057 mean_circ: 0.948835
Ran for 187050 frames Cell Events: 7077 mean_circ: 0.948829
Ran for 187550 frames Cell Events: 7113 mean_circ: 0.948848
Ran for 188550 frames Cell Events: 7164 mean_circ: 0.948844
Ran for 189050 frames Cell Events: 7178 mean_circ: 0.948846
Ran for 189550 frames Cell Events: 7199 mean_circ: 0.948862
Ran for 190050 frames Cell Events: 7214 mean_circ: 0.948856
Ran for 191050 frames Cell Events: 7253 mean_circ: 0.94885
Ran for 191550 frames Cell Events: 7275 mean_circ: 0.948773
Ran for 192550 frames Cell Events: 7328 mean_circ: 0.948832
Ran for 194550 frames Cell Events: 7417 mean_circ: 0.948857
Ran for 195050 frames Cell Events: 7429 mean_circ: 0.948882
Ran for 196550 frames Cell Events: 7495 mean_circ: 0.948877
Ran for 197050 frames Cell Events: 7525 mean_circ: 0.948917
Ran for 197550 frames Cell Events: 7546 mean_circ: 0.948935
Ran for 198050 frames Cell Events: 7568 mean_circ: 0.94895
Ran for 198550 frames Cell Events: 7588 mean_circ: 0.948926
Ran for 199050 frames Cell Events: 7608 mean_circ: 0.9489
Ran for 199550 frames Cell Events: 7617 mean_circ: 0.948878
Ran for 200050 frames Cell Events: 7649 mean_circ: 0.948903
Ran for 201050 frames Cell Events: 7700 mean_circ: 0.948959
Ran for 202050 frames Cell Events: 7738 mean_circ: 0.948927
Ran for 202550 frames Cell Events: 7770 mean_circ: 0.948903
Ran for 203050 frames Cell Events: 7804 mean_circ: 0.948908
Ran for 204550 frames Cell Events: 7854 mean_circ: 0.948817
Ran for 206050 frames Cell Events: 7922 mean_circ: 0.948822
Ran for 207050 frames Cell Events: 7951 mean_circ: 0.948862
Ran for 207550 frames Cell Events: 7969 mean_circ: 0.948862
Ran for 208050 frames Cell Events: 7989 mean_circ: 0.948859
Ran for 210050 frames Cell Events: 8060 mean_circ: 0.948884
Ran for 210550 frames Cell Events: 8091 mean_circ: 0.948911
Ran for 211050 frames Cell Events: 8105 mean_circ: 0.948905
Ran for 212550 frames Cell Events: 8177 mean_circ: 0.948886
Ran for 213550 frames Cell Events: 8214 mean_circ: 0.948858
Ran for 214050 frames Cell Events: 8241 mean_circ: 0.948863
Ran for 214550 frames Cell Events: 8266 mean_circ: 0.948878
Ran for 215050 frames Cell Events: 8284 mean_circ: 0.948904
Ran for 216050 frames Cell Events: 8335 mean_circ: 0.948917
Ran for 217050 frames Cell Events: 8380 mean_circ: 0.94893
Ran for 219550 frames Cell Events: 8500 mean_circ: 0.948928
Ran for 220050 frames Cell Events: 8520 mean_circ: 0.948937
Ran for 220550 frames Cell Events: 8535 mean_circ: 0.948949
Ran for 221550 frames Cell Events: 8577 mean_circ: 0.948969
Ran for 222050 frames Cell Events: 8609 mean_circ: 0.94904
Ran for 222550 frames Cell Events: 8631 mean_circ: 0.949024
Ran for 223550 frames Cell Events: 8685 mean_circ: 0.949021
Ran for 224050 frames Cell Events: 8708 mean_circ: 0.949087
Ran for 224550 frames Cell Events: 8723 mean_circ: 0.949105
Ran for 225050 frames Cell Events: 8742 mean_circ: 0.949106
Ran for 225550 frames Cell Events: 8761 mean_circ: 0.949105
Ran for 226550 frames Cell Events: 8798 mean_circ: 0.949097
Ran for 227550 frames Cell Events: 8837 mean_circ: 0.949106
Ran for 228050 frames Cell Events: 8861 mean_circ: 0.949106
Ran for 228550 frames Cell Events: 8883 mean_circ: 0.949093
Ran for 229050 frames Cell Events: 8894 mean_circ: 0.949065
Ran for 229550 frames Cell Events: 8925 mean_circ: 0.949058
Ran for 230050 frames Cell Events: 8935 mean_circ: 0.949068
Ran for 230550 frames Cell Events: 8961 mean_circ: 0.949041
Ran for 231050 frames Cell Events: 8978 mean_circ: 0.949042
Ran for 231550 frames Cell Events: 9001 mean_circ: 0.949052
Ran for 232050 frames Cell Events: 9022 mean_circ: 0.949036
Ran for 233050 frames Cell Events: 9062 mean_circ: 0.949069
Ran for 234050 frames Cell Events: 9102 mean_circ: 0.949077
Ran for 235050 frames Cell Events: 9152 mean_circ: 0.949056
Ran for 235550 frames Cell Events: 9171 mean_circ: 0.949046
Ran for 236550 frames Cell Events: 9216 mean_circ: 0.949053
Ran for 237050 frames Cell Events: 9242 mean_circ: 0.949061
Ran for 237550 frames Cell Events: 9261 mean_circ: 0.949077
Ran for 240550 frames Cell Events: 9405 mean_circ: 0.949012
Ran for 241050 frames Cell Events: 9424 mean_circ: 0.949017
Ran for 241550 frames Cell Events: 9447 mean_circ: 0.948986
End this measurment..
Stopping time: 4.79631e+008
****************************************
Measurement finished
Mean Circularitiy: 0.948893
Mean Area: 621.691
Mean FL-1 intensity: 3290.88
Mean FL-2 intensity: 3998.43
Mean FL-3 intensity: 0
frames processed: 241659
Total Time: 120945ms
Number of Runs (new bg img) 0
Frames collected: 241721
Skipped frames: 10862
Rescued Frames: 1816980
Samples DAQ: 60430565
Samples written: 120860284
Samples per Channel: 60430142
FPS: 1998.6
Samples per second: 499648
Expected samples/s: 500000
Events: 9459
Events per second: 78.2089
jumped back (PMT): 6688
#Frames read, frequency
# 0 frames :335539
# 1 frames :127977
# 2 frames :6102
# 3 frames :2106
# 4 frames :830
# 5 frames :2677
# 6 frames :2219
# 7 frames :1298
# 8 frames :648
# 9 frames :457
# 10 frames :1142
# 11 frames :628
# 12 frames :464
# 13 frames :240
# 14 frames :201
# 15 frames :171
# 16 frames :141
# 17 frames :132
# 18 frames :83
# 19 frames :70
# 20 frames :40
# 21 frames :39
# 22 frames :32
# 23 frames :25
# 24 frames :28
# 25 frames :21
# 26 frames :14
# 27 frames :21
# 28 frames :22
# 29 frames :12
# 30 frames :4
# 31 frames :7
# 32 frames :3
# 33 frames :3
# 35 frames :1
# 36 frames :1
# 37 frames :1
# 38 frames :2
# 39 frames :2
# 40 frames :2
# 41 frames :1
# 43 frames :1
# 44 frames :1
# 47 frames :1
# 62 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0536
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0