Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_19.22.17
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "bf2732deff2917414558a1d093dc207e",
"name": "M1_20170619_1626_dsRNA_U7_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:22:37"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8022 (max 8024) in '8022'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1033
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 4.79757e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 16 mean_circ: 0.62999
Ran for 1550 frames Cell Events: 49 mean_circ: 0.926391
Ran for 3050 frames Cell Events: 111 mean_circ: 0.935356
Ran for 3550 frames Cell Events: 129 mean_circ: 0.931239
Ran for 4050 frames Cell Events: 146 mean_circ: 0.931547
Ran for 4550 frames Cell Events: 165 mean_circ: 0.933074
Ran for 5050 frames Cell Events: 183 mean_circ: 0.933143
Ran for 5550 frames Cell Events: 205 mean_circ: 0.931619
Ran for 6050 frames Cell Events: 218 mean_circ: 0.932164
Ran for 6550 frames Cell Events: 236 mean_circ: 0.931037
Ran for 9050 frames Cell Events: 315 mean_circ: 0.933156
Ran for 9550 frames Cell Events: 328 mean_circ: 0.933197
Ran for 11050 frames Cell Events: 401 mean_circ: 0.934792
Ran for 11550 frames Cell Events: 424 mean_circ: 0.935145
Ran for 12050 frames Cell Events: 458 mean_circ: 0.935406
Ran for 12550 frames Cell Events: 479 mean_circ: 0.935775
Ran for 13550 frames Cell Events: 535 mean_circ: 0.935313
Ran for 14550 frames Cell Events: 581 mean_circ: 0.935303
Ran for 16050 frames Cell Events: 658 mean_circ: 0.935503
Ran for 16550 frames Cell Events: 680 mean_circ: 0.93551
Ran for 17050 frames Cell Events: 704 mean_circ: 0.935035
Ran for 18050 frames Cell Events: 752 mean_circ: 0.93489
Ran for 20050 frames Cell Events: 840 mean_circ: 0.935308
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Ran for 38050 frames Cell Events: 1590 mean_circ: 0.936058
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Ran for 39050 frames Cell Events: 1635 mean_circ: 0.936104
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Ran for 40050 frames Cell Events: 1667 mean_circ: 0.936046
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Ran for 47050 frames Cell Events: 1926 mean_circ: 0.936031
Ran for 48050 frames Cell Events: 1968 mean_circ: 0.936078
Ran for 48550 frames Cell Events: 1994 mean_circ: 0.93609
Ran for 49050 frames Cell Events: 2010 mean_circ: 0.936065
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Ran for 64050 frames Cell Events: 2646 mean_circ: 0.935675
Ran for 65550 frames Cell Events: 2711 mean_circ: 0.935711
Ran for 66050 frames Cell Events: 2732 mean_circ: 0.935616
Ran for 66550 frames Cell Events: 2751 mean_circ: 0.935698
Ran for 67050 frames Cell Events: 2770 mean_circ: 0.935711
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Ran for 68550 frames Cell Events: 2847 mean_circ: 0.935768
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Ran for 70050 frames Cell Events: 2905 mean_circ: 0.935741
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Ran for 71550 frames Cell Events: 2991 mean_circ: 0.935478
Ran for 72050 frames Cell Events: 3014 mean_circ: 0.935419
Ran for 72550 frames Cell Events: 3041 mean_circ: 0.935404
Ran for 73050 frames Cell Events: 3063 mean_circ: 0.935259
Ran for 74050 frames Cell Events: 3116 mean_circ: 0.935228
Ran for 75050 frames Cell Events: 3174 mean_circ: 0.935271
Ran for 75550 frames Cell Events: 3202 mean_circ: 0.9353
Ran for 76050 frames Cell Events: 3230 mean_circ: 0.935343
Ran for 76550 frames Cell Events: 3252 mean_circ: 0.935438
Ran for 77050 frames Cell Events: 3279 mean_circ: 0.935458
Ran for 77550 frames Cell Events: 3293 mean_circ: 0.935489
Ran for 78050 frames Cell Events: 3317 mean_circ: 0.93551
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Ran for 79050 frames Cell Events: 3343 mean_circ: 0.935505
Ran for 80050 frames Cell Events: 3390 mean_circ: 0.935436
Ran for 80550 frames Cell Events: 3413 mean_circ: 0.935517
Ran for 81050 frames Cell Events: 3441 mean_circ: 0.935581
Ran for 81550 frames Cell Events: 3471 mean_circ: 0.935557
Ran for 82050 frames Cell Events: 3502 mean_circ: 0.935636
Ran for 82550 frames Cell Events: 3523 mean_circ: 0.935593
Ran for 83550 frames Cell Events: 3559 mean_circ: 0.935588
Ran for 84050 frames Cell Events: 3595 mean_circ: 0.935577
Ran for 84550 frames Cell Events: 3614 mean_circ: 0.935608
Ran for 85550 frames Cell Events: 3658 mean_circ: 0.935627
Ran for 86050 frames Cell Events: 3695 mean_circ: 0.935695
Ran for 87050 frames Cell Events: 3748 mean_circ: 0.935843
Ran for 87550 frames Cell Events: 3770 mean_circ: 0.935854
Ran for 88050 frames Cell Events: 3794 mean_circ: 0.935864
Ran for 88550 frames Cell Events: 3822 mean_circ: 0.935827
Ran for 89050 frames Cell Events: 3842 mean_circ: 0.935891
Ran for 89550 frames Cell Events: 3862 mean_circ: 0.935936
Ran for 90050 frames Cell Events: 3885 mean_circ: 0.935917
Ran for 90550 frames Cell Events: 3900 mean_circ: 0.93594
Ran for 91050 frames Cell Events: 3921 mean_circ: 0.935809
Ran for 92050 frames Cell Events: 3970 mean_circ: 0.935805
Ran for 93050 frames Cell Events: 4025 mean_circ: 0.93574
Ran for 93550 frames Cell Events: 4056 mean_circ: 0.935654
Ran for 94050 frames Cell Events: 4072 mean_circ: 0.935623
Ran for 94550 frames Cell Events: 4090 mean_circ: 0.935632
Ran for 95050 frames Cell Events: 4102 mean_circ: 0.935673
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Ran for 96050 frames Cell Events: 4137 mean_circ: 0.935645
Ran for 96550 frames Cell Events: 4163 mean_circ: 0.93557
Ran for 97050 frames Cell Events: 4188 mean_circ: 0.93565
Ran for 97550 frames Cell Events: 4206 mean_circ: 0.935592
Ran for 98050 frames Cell Events: 4226 mean_circ: 0.935607
Ran for 98550 frames Cell Events: 4252 mean_circ: 0.935622
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Ran for 104050 frames Cell Events: 4469 mean_circ: 0.935593
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Ran for 106550 frames Cell Events: 4597 mean_circ: 0.935385
Ran for 107050 frames Cell Events: 4622 mean_circ: 0.935346
Ran for 108050 frames Cell Events: 4662 mean_circ: 0.935282
Ran for 108550 frames Cell Events: 4674 mean_circ: 0.93531
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Ran for 109550 frames Cell Events: 4720 mean_circ: 0.935214
Ran for 110050 frames Cell Events: 4730 mean_circ: 0.935212
Ran for 111050 frames Cell Events: 4772 mean_circ: 0.935216
Ran for 112050 frames Cell Events: 4789 mean_circ: 0.935161
Ran for 112550 frames Cell Events: 4807 mean_circ: 0.935132
Ran for 113050 frames Cell Events: 4832 mean_circ: 0.935169
Ran for 113550 frames Cell Events: 4848 mean_circ: 0.935147
Ran for 114050 frames Cell Events: 4863 mean_circ: 0.935172
Ran for 114550 frames Cell Events: 4888 mean_circ: 0.935196
Ran for 115050 frames Cell Events: 4907 mean_circ: 0.935246
Ran for 115550 frames Cell Events: 4933 mean_circ: 0.935239
Ran for 117050 frames Cell Events: 4996 mean_circ: 0.935274
Ran for 119050 frames Cell Events: 5091 mean_circ: 0.935349
Ran for 120550 frames Cell Events: 5150 mean_circ: 0.935267
Ran for 121050 frames Cell Events: 5162 mean_circ: 0.935283
Ran for 122550 frames Cell Events: 5229 mean_circ: 0.935317
Ran for 123050 frames Cell Events: 5255 mean_circ: 0.935312
Ran for 123550 frames Cell Events: 5278 mean_circ: 0.935304
Ran for 124550 frames Cell Events: 5315 mean_circ: 0.935326
Ran for 125050 frames Cell Events: 5332 mean_circ: 0.935274
Ran for 125550 frames Cell Events: 5349 mean_circ: 0.935304
Ran for 126050 frames Cell Events: 5373 mean_circ: 0.935296
Ran for 126550 frames Cell Events: 5396 mean_circ: 0.935267
Ran for 127050 frames Cell Events: 5414 mean_circ: 0.935288
Ran for 127550 frames Cell Events: 5432 mean_circ: 0.9353
Ran for 128550 frames Cell Events: 5464 mean_circ: 0.935353
Ran for 129050 frames Cell Events: 5486 mean_circ: 0.935326
Ran for 129550 frames Cell Events: 5508 mean_circ: 0.93532
Ran for 130050 frames Cell Events: 5521 mean_circ: 0.935312
Ran for 130550 frames Cell Events: 5546 mean_circ: 0.935328
Ran for 132050 frames Cell Events: 5617 mean_circ: 0.935345
Ran for 132550 frames Cell Events: 5643 mean_circ: 0.935261
Ran for 133550 frames Cell Events: 5652 mean_circ: 0.935253
Ran for 135050 frames Cell Events: 5729 mean_circ: 0.935242
Ran for 135550 frames Cell Events: 5752 mean_circ: 0.935217
Ran for 136050 frames Cell Events: 5760 mean_circ: 0.935214
Ran for 137050 frames Cell Events: 5804 mean_circ: 0.935224
Ran for 137550 frames Cell Events: 5822 mean_circ: 0.935231
Ran for 138050 frames Cell Events: 5839 mean_circ: 0.935222
Ran for 138550 frames Cell Events: 5873 mean_circ: 0.935201
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Ran for 141050 frames Cell Events: 5988 mean_circ: 0.935192
Ran for 142550 frames Cell Events: 6046 mean_circ: 0.935123
Ran for 143050 frames Cell Events: 6061 mean_circ: 0.935174
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Ran for 146050 frames Cell Events: 6200 mean_circ: 0.935176
Ran for 146550 frames Cell Events: 6215 mean_circ: 0.935194
Ran for 147050 frames Cell Events: 6237 mean_circ: 0.935219
Ran for 147550 frames Cell Events: 6252 mean_circ: 0.93518
Ran for 148050 frames Cell Events: 6269 mean_circ: 0.935205
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Ran for 151050 frames Cell Events: 6411 mean_circ: 0.935116
Ran for 152050 frames Cell Events: 6453 mean_circ: 0.935091
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Ran for 166550 frames Cell Events: 7101 mean_circ: 0.935061
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Ran for 168050 frames Cell Events: 7160 mean_circ: 0.935064
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Ran for 170050 frames Cell Events: 7239 mean_circ: 0.935116
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Ran for 171050 frames Cell Events: 7273 mean_circ: 0.935115
Ran for 171550 frames Cell Events: 7292 mean_circ: 0.935062
Ran for 172050 frames Cell Events: 7311 mean_circ: 0.935056
Ran for 172550 frames Cell Events: 7333 mean_circ: 0.935053
Ran for 174050 frames Cell Events: 7421 mean_circ: 0.935011
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Ran for 178050 frames Cell Events: 7564 mean_circ: 0.935009
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Ran for 179550 frames Cell Events: 7628 mean_circ: 0.934971
Ran for 180550 frames Cell Events: 7664 mean_circ: 0.93499
Ran for 181050 frames Cell Events: 7701 mean_circ: 0.934939
Ran for 182050 frames Cell Events: 7737 mean_circ: 0.934965
Ran for 182550 frames Cell Events: 7764 mean_circ: 0.934948
Ran for 183050 frames Cell Events: 7775 mean_circ: 0.934968
Ran for 183550 frames Cell Events: 7799 mean_circ: 0.934963
Ran for 184050 frames Cell Events: 7831 mean_circ: 0.934928
Ran for 184550 frames Cell Events: 7850 mean_circ: 0.934964
Ran for 185550 frames Cell Events: 7888 mean_circ: 0.934961
Ran for 186050 frames Cell Events: 7908 mean_circ: 0.934962
Ran for 186550 frames Cell Events: 7927 mean_circ: 0.934975
Ran for 187050 frames Cell Events: 7941 mean_circ: 0.934979
Ran for 187550 frames Cell Events: 7966 mean_circ: 0.934959
Ran for 188550 frames Cell Events: 8016 mean_circ: 0.934931
End this measurment..
Stopping time: 4.79852e+008
****************************************
Measurement finished
Mean Circularitiy: 0.934925
Mean Area: 633.074
Mean FL-1 intensity: 3120
Mean FL-2 intensity: 4309.71
Mean FL-3 intensity: 0
frames processed: 186635
Total Time: 94456.5ms
Number of Runs (new bg img) 0
Frames collected: 188769
Skipped frames: 12997
Rescued Frames: 1881726
Samples DAQ: 47192268
Samples written: 94384418
Samples per Channel: 47192209
FPS: 1998.47
Samples per second: 499618
Expected samples/s: 500000
Events: 8024
Events per second: 84.9491
jumped back (PMT): 16
#Frames read, frequency
# 0 frames :255610
# 1 frames :107223
# 2 frames :3712
# 3 frames :1496
# 4 frames :424
# 5 frames :3254
# 6 frames :1954
# 7 frames :1322
# 8 frames :577
# 9 frames :322
# 10 frames :409
# 11 frames :345
# 12 frames :255
# 13 frames :151
# 14 frames :93
# 15 frames :65
# 16 frames :54
# 17 frames :39
# 18 frames :36
# 19 frames :36
# 20 frames :29
# 21 frames :16
# 22 frames :18
# 23 frames :7
# 24 frames :13
# 25 frames :4
# 26 frames :4
# 27 frames :6
# 28 frames :4
# 29 frames :4
# 30 frames :2
# 31 frames :1
# 32 frames :2
# 33 frames :3
# 34 frames :2
# 36 frames :1
# 38 frames :1
# 41 frames :1
# 43 frames :2
# 47 frames :1
# 49 frames :1
# 72 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1033
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0