Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_19.26.05
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "29847b2d49f2411eacb3495563f77dfe",
"name": "M1_20170619_1720_set1_dsRNA_37b.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:26:26"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8169 (max 8170) in '8169'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9749
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 4.82975e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 22 mean_circ: 0.927866
Ran for 1550 frames Cell Events: 61 mean_circ: 0.915742
Ran for 2550 frames Cell Events: 87 mean_circ: 0.917639
Ran for 3550 frames Cell Events: 116 mean_circ: 0.917583
Ran for 4050 frames Cell Events: 135 mean_circ: 0.917797
Ran for 4550 frames Cell Events: 166 mean_circ: 0.917587
Ran for 5050 frames Cell Events: 175 mean_circ: 0.916501
Ran for 6550 frames Cell Events: 221 mean_circ: 0.916017
Ran for 7050 frames Cell Events: 235 mean_circ: 0.916248
Ran for 7550 frames Cell Events: 245 mean_circ: 0.91627
Ran for 8050 frames Cell Events: 260 mean_circ: 0.915712
Ran for 8550 frames Cell Events: 281 mean_circ: 0.916319
Ran for 9550 frames Cell Events: 310 mean_circ: 0.916216
Ran for 10550 frames Cell Events: 342 mean_circ: 0.916637
Ran for 11050 frames Cell Events: 356 mean_circ: 0.916201
Ran for 11550 frames Cell Events: 371 mean_circ: 0.916382
Ran for 13050 frames Cell Events: 425 mean_circ: 0.917891
Ran for 13550 frames Cell Events: 444 mean_circ: 0.918352
Ran for 14050 frames Cell Events: 461 mean_circ: 0.91871
Ran for 14550 frames Cell Events: 477 mean_circ: 0.918533
Ran for 15050 frames Cell Events: 502 mean_circ: 0.919404
Ran for 16050 frames Cell Events: 544 mean_circ: 0.920069
Ran for 17050 frames Cell Events: 583 mean_circ: 0.920093
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Ran for 25050 frames Cell Events: 935 mean_circ: 0.920341
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Ran for 38050 frames Cell Events: 1447 mean_circ: 0.919982
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Ran for 40550 frames Cell Events: 1530 mean_circ: 0.920245
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Ran for 51550 frames Cell Events: 1952 mean_circ: 0.920591
Ran for 52050 frames Cell Events: 1969 mean_circ: 0.920754
Ran for 52550 frames Cell Events: 1994 mean_circ: 0.920659
Ran for 53050 frames Cell Events: 2010 mean_circ: 0.920664
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Ran for 70550 frames Cell Events: 2720 mean_circ: 0.921024
Ran for 71050 frames Cell Events: 2745 mean_circ: 0.921016
Ran for 72550 frames Cell Events: 2828 mean_circ: 0.920892
Ran for 73550 frames Cell Events: 2855 mean_circ: 0.920816
Ran for 74050 frames Cell Events: 2882 mean_circ: 0.920815
Ran for 74550 frames Cell Events: 2901 mean_circ: 0.920789
Ran for 75050 frames Cell Events: 2924 mean_circ: 0.920853
Ran for 76050 frames Cell Events: 2969 mean_circ: 0.920973
Ran for 76550 frames Cell Events: 2990 mean_circ: 0.921038
Ran for 77050 frames Cell Events: 3006 mean_circ: 0.920929
Ran for 78550 frames Cell Events: 3062 mean_circ: 0.921025
Ran for 79050 frames Cell Events: 3083 mean_circ: 0.920956
Ran for 79550 frames Cell Events: 3102 mean_circ: 0.920878
Ran for 80050 frames Cell Events: 3122 mean_circ: 0.920823
Ran for 80550 frames Cell Events: 3139 mean_circ: 0.920797
Ran for 81050 frames Cell Events: 3164 mean_circ: 0.920718
Ran for 81550 frames Cell Events: 3172 mean_circ: 0.920721
Ran for 82050 frames Cell Events: 3189 mean_circ: 0.920681
Ran for 82550 frames Cell Events: 3212 mean_circ: 0.920635
Ran for 83050 frames Cell Events: 3242 mean_circ: 0.920557
Ran for 83550 frames Cell Events: 3256 mean_circ: 0.92059
Ran for 84050 frames Cell Events: 3275 mean_circ: 0.920467
Ran for 84550 frames Cell Events: 3298 mean_circ: 0.92047
Ran for 85050 frames Cell Events: 3314 mean_circ: 0.92051
Ran for 86550 frames Cell Events: 3380 mean_circ: 0.920671
Ran for 87050 frames Cell Events: 3391 mean_circ: 0.920636
Ran for 87550 frames Cell Events: 3402 mean_circ: 0.920658
Ran for 88050 frames Cell Events: 3424 mean_circ: 0.920689
Ran for 88550 frames Cell Events: 3447 mean_circ: 0.920729
Ran for 89550 frames Cell Events: 3480 mean_circ: 0.920763
Ran for 91050 frames Cell Events: 3529 mean_circ: 0.920746
Ran for 91550 frames Cell Events: 3546 mean_circ: 0.920768
Ran for 92050 frames Cell Events: 3550 mean_circ: 0.920762
Ran for 92550 frames Cell Events: 3561 mean_circ: 0.920771
Ran for 93050 frames Cell Events: 3580 mean_circ: 0.920773
Ran for 93550 frames Cell Events: 3597 mean_circ: 0.920677
Ran for 94550 frames Cell Events: 3641 mean_circ: 0.920722
Ran for 95050 frames Cell Events: 3656 mean_circ: 0.920715
Ran for 95550 frames Cell Events: 3669 mean_circ: 0.920713
Ran for 96050 frames Cell Events: 3690 mean_circ: 0.920735
Ran for 97550 frames Cell Events: 3753 mean_circ: 0.920685
Ran for 98550 frames Cell Events: 3791 mean_circ: 0.920592
Ran for 99550 frames Cell Events: 3836 mean_circ: 0.920638
Ran for 100050 frames Cell Events: 3855 mean_circ: 0.920694
Ran for 101550 frames Cell Events: 3905 mean_circ: 0.920689
Ran for 102050 frames Cell Events: 3916 mean_circ: 0.920656
Ran for 102550 frames Cell Events: 3928 mean_circ: 0.920641
Ran for 105050 frames Cell Events: 4038 mean_circ: 0.92067
Ran for 106050 frames Cell Events: 4082 mean_circ: 0.920592
Ran for 106550 frames Cell Events: 4102 mean_circ: 0.920643
Ran for 107050 frames Cell Events: 4114 mean_circ: 0.920665
Ran for 108050 frames Cell Events: 4159 mean_circ: 0.920653
Ran for 109550 frames Cell Events: 4209 mean_circ: 0.920677
Ran for 110550 frames Cell Events: 4250 mean_circ: 0.920587
Ran for 111050 frames Cell Events: 4263 mean_circ: 0.920636
Ran for 111550 frames Cell Events: 4278 mean_circ: 0.920635
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Ran for 112550 frames Cell Events: 4318 mean_circ: 0.920657
Ran for 113050 frames Cell Events: 4337 mean_circ: 0.920657
Ran for 115550 frames Cell Events: 4450 mean_circ: 0.920617
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Ran for 120050 frames Cell Events: 4649 mean_circ: 0.920609
Ran for 120550 frames Cell Events: 4667 mean_circ: 0.920625
Ran for 122050 frames Cell Events: 4731 mean_circ: 0.920504
Ran for 123550 frames Cell Events: 4789 mean_circ: 0.920502
Ran for 124050 frames Cell Events: 4814 mean_circ: 0.920427
Ran for 124550 frames Cell Events: 4841 mean_circ: 0.920429
Ran for 125050 frames Cell Events: 4853 mean_circ: 0.920383
Ran for 125550 frames Cell Events: 4868 mean_circ: 0.920406
Ran for 126550 frames Cell Events: 4901 mean_circ: 0.920401
Ran for 127550 frames Cell Events: 4936 mean_circ: 0.920551
Ran for 128550 frames Cell Events: 4978 mean_circ: 0.920518
Ran for 129050 frames Cell Events: 5002 mean_circ: 0.920463
Ran for 129550 frames Cell Events: 5020 mean_circ: 0.920544
Ran for 130050 frames Cell Events: 5032 mean_circ: 0.920557
Ran for 130550 frames Cell Events: 5057 mean_circ: 0.920542
Ran for 131050 frames Cell Events: 5067 mean_circ: 0.920586
Ran for 131550 frames Cell Events: 5085 mean_circ: 0.920538
Ran for 132050 frames Cell Events: 5095 mean_circ: 0.920524
Ran for 133050 frames Cell Events: 5159 mean_circ: 0.920559
Ran for 133550 frames Cell Events: 5183 mean_circ: 0.920534
Ran for 134050 frames Cell Events: 5200 mean_circ: 0.920523
Ran for 134550 frames Cell Events: 5227 mean_circ: 0.920575
Ran for 135050 frames Cell Events: 5255 mean_circ: 0.920636
Ran for 136050 frames Cell Events: 5301 mean_circ: 0.920628
Ran for 137050 frames Cell Events: 5346 mean_circ: 0.920702
Ran for 138050 frames Cell Events: 5399 mean_circ: 0.92064
Ran for 139050 frames Cell Events: 5443 mean_circ: 0.92073
Ran for 139550 frames Cell Events: 5468 mean_circ: 0.92075
Ran for 140550 frames Cell Events: 5513 mean_circ: 0.920779
Ran for 141550 frames Cell Events: 5552 mean_circ: 0.920834
Ran for 142050 frames Cell Events: 5576 mean_circ: 0.920855
Ran for 143050 frames Cell Events: 5610 mean_circ: 0.920858
Ran for 143550 frames Cell Events: 5627 mean_circ: 0.92085
Ran for 144050 frames Cell Events: 5646 mean_circ: 0.920813
Ran for 146050 frames Cell Events: 5745 mean_circ: 0.920873
Ran for 146550 frames Cell Events: 5766 mean_circ: 0.920899
Ran for 147050 frames Cell Events: 5789 mean_circ: 0.920884
Ran for 147550 frames Cell Events: 5810 mean_circ: 0.920929
Ran for 148550 frames Cell Events: 5844 mean_circ: 0.920896
Ran for 149050 frames Cell Events: 5865 mean_circ: 0.920916
Ran for 149550 frames Cell Events: 5891 mean_circ: 0.920967
Ran for 150050 frames Cell Events: 5916 mean_circ: 0.920999
Ran for 150550 frames Cell Events: 5927 mean_circ: 0.920992
Ran for 151050 frames Cell Events: 5946 mean_circ: 0.920988
Ran for 152050 frames Cell Events: 5995 mean_circ: 0.920992
Ran for 153050 frames Cell Events: 6032 mean_circ: 0.920934
Ran for 153550 frames Cell Events: 6059 mean_circ: 0.9209
Ran for 154050 frames Cell Events: 6069 mean_circ: 0.92089
Ran for 154550 frames Cell Events: 6094 mean_circ: 0.920862
Ran for 155050 frames Cell Events: 6115 mean_circ: 0.920852
Ran for 155550 frames Cell Events: 6143 mean_circ: 0.920858
Ran for 156050 frames Cell Events: 6161 mean_circ: 0.920833
Ran for 156550 frames Cell Events: 6184 mean_circ: 0.920823
Ran for 157050 frames Cell Events: 6208 mean_circ: 0.920898
Ran for 159050 frames Cell Events: 6298 mean_circ: 0.920909
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Ran for 160050 frames Cell Events: 6330 mean_circ: 0.920984
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Ran for 162550 frames Cell Events: 6449 mean_circ: 0.921045
Ran for 163050 frames Cell Events: 6461 mean_circ: 0.921054
Ran for 163550 frames Cell Events: 6487 mean_circ: 0.92101
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Ran for 172050 frames Cell Events: 6801 mean_circ: 0.920991
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Ran for 176550 frames Cell Events: 6983 mean_circ: 0.920844
Ran for 177050 frames Cell Events: 6998 mean_circ: 0.920831
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Ran for 181550 frames Cell Events: 7147 mean_circ: 0.920734
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Ran for 183050 frames Cell Events: 7218 mean_circ: 0.920822
Ran for 183550 frames Cell Events: 7238 mean_circ: 0.920831
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Ran for 185050 frames Cell Events: 7315 mean_circ: 0.920866
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Ran for 198050 frames Cell Events: 7847 mean_circ: 0.920954
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Ran for 199050 frames Cell Events: 7877 mean_circ: 0.920954
Ran for 199550 frames Cell Events: 7897 mean_circ: 0.920942
Ran for 200050 frames Cell Events: 7905 mean_circ: 0.920946
Ran for 200550 frames Cell Events: 7914 mean_circ: 0.920963
Ran for 201550 frames Cell Events: 7963 mean_circ: 0.920965
Ran for 202550 frames Cell Events: 8012 mean_circ: 0.920969
Ran for 204050 frames Cell Events: 8072 mean_circ: 0.920989
Ran for 205050 frames Cell Events: 8101 mean_circ: 0.921017
Ran for 205550 frames Cell Events: 8124 mean_circ: 0.921038
Ran for 206050 frames Cell Events: 8144 mean_circ: 0.92105
End this measurment..
Stopping time: 4.83078e+008
****************************************
Measurement finished
Mean Circularitiy: 0.921044
Mean Area: 711.94
Mean FL-1 intensity: 3145.18
Mean FL-2 intensity: 3529.16
Mean FL-3 intensity: 0
frames processed: 206350
Total Time: 103349ms
Number of Runs (new bg img) 0
Frames collected: 206535
Skipped frames: 17330
Rescued Frames: 2492761
Samples DAQ: 51633694
Samples written: 103267124
Samples per Channel: 51633562
FPS: 1998.42
Samples per second: 499604
Expected samples/s: 500000
Events: 8170
Events per second: 79.0525
jumped back (PMT): 5508
#Frames read, frequency
# 0 frames :291678
# 1 frames :104060
# 2 frames :5121
# 3 frames :1761
# 4 frames :886
# 5 frames :1979
# 6 frames :1873
# 7 frames :1230
# 8 frames :742
# 9 frames :453
# 10 frames :903
# 11 frames :545
# 12 frames :433
# 13 frames :289
# 14 frames :214
# 15 frames :179
# 16 frames :142
# 17 frames :118
# 18 frames :96
# 19 frames :71
# 20 frames :42
# 21 frames :37
# 22 frames :36
# 23 frames :28
# 24 frames :24
# 25 frames :25
# 26 frames :13
# 27 frames :4
# 28 frames :10
# 29 frames :5
# 30 frames :11
# 31 frames :7
# 32 frames :3
# 33 frames :1
# 34 frames :1
# 35 frames :2
# 36 frames :2
# 37 frames :3
# 38 frames :2
# 39 frames :1
# 40 frames :4
# 42 frames :1
# 46 frames :1
# 51 frames :1
# 59 frames :1
# 75 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9749
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0