Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_19.28.32
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "25a28cdc762b4c7c639a84eb9983b797",
"name": "M1_20170619_1747_dsRNA_26B_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:28:57"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9999 (max 10000) in
'9999'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9751
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 4.84589e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1550 frames Cell Events: 91 mean_circ: 0.931769
Ran for 3050 frames Cell Events: 181 mean_circ: 0.930537
Ran for 4550 frames Cell Events: 264 mean_circ: 0.933392
Ran for 5550 frames Cell Events: 320 mean_circ: 0.933867
Ran for 6050 frames Cell Events: 346 mean_circ: 0.933679
Ran for 6550 frames Cell Events: 362 mean_circ: 0.933758
Ran for 7050 frames Cell Events: 378 mean_circ: 0.934464
Ran for 9050 frames Cell Events: 472 mean_circ: 0.932855
Ran for 9550 frames Cell Events: 504 mean_circ: 0.933381
Ran for 10550 frames Cell Events: 581 mean_circ: 0.933025
Ran for 11050 frames Cell Events: 610 mean_circ: 0.933181
Ran for 12050 frames Cell Events: 659 mean_circ: 0.933345
Ran for 13050 frames Cell Events: 712 mean_circ: 0.933836
Ran for 14050 frames Cell Events: 761 mean_circ: 0.933425
Ran for 14550 frames Cell Events: 790 mean_circ: 0.933418
Ran for 15050 frames Cell Events: 807 mean_circ: 0.933176
Ran for 15550 frames Cell Events: 826 mean_circ: 0.933363
Ran for 17550 frames Cell Events: 939 mean_circ: 0.932734
Ran for 18050 frames Cell Events: 963 mean_circ: 0.93281
Ran for 18550 frames Cell Events: 981 mean_circ: 0.93287
Ran for 19050 frames Cell Events: 996 mean_circ: 0.932725
Ran for 19550 frames Cell Events: 1019 mean_circ: 0.932842
Ran for 20050 frames Cell Events: 1043 mean_circ: 0.93259
Ran for 20550 frames Cell Events: 1066 mean_circ: 0.932627
Ran for 21050 frames Cell Events: 1087 mean_circ: 0.93228
Ran for 21550 frames Cell Events: 1107 mean_circ: 0.932182
Ran for 22050 frames Cell Events: 1124 mean_circ: 0.932323
Ran for 22550 frames Cell Events: 1144 mean_circ: 0.931827
Ran for 23050 frames Cell Events: 1159 mean_circ: 0.931655
Ran for 24050 frames Cell Events: 1208 mean_circ: 0.931906
Ran for 24550 frames Cell Events: 1233 mean_circ: 0.932024
Ran for 25050 frames Cell Events: 1249 mean_circ: 0.932082
Ran for 25550 frames Cell Events: 1273 mean_circ: 0.932418
Ran for 26550 frames Cell Events: 1308 mean_circ: 0.932472
Ran for 27050 frames Cell Events: 1332 mean_circ: 0.932557
Ran for 28550 frames Cell Events: 1397 mean_circ: 0.932576
Ran for 29050 frames Cell Events: 1415 mean_circ: 0.932619
Ran for 29550 frames Cell Events: 1438 mean_circ: 0.932863
Ran for 30050 frames Cell Events: 1452 mean_circ: 0.932818
Ran for 30550 frames Cell Events: 1473 mean_circ: 0.932754
Ran for 31050 frames Cell Events: 1493 mean_circ: 0.932716
Ran for 33050 frames Cell Events: 1636 mean_circ: 0.932906
Ran for 34550 frames Cell Events: 1788 mean_circ: 0.932899
Ran for 36050 frames Cell Events: 1908 mean_circ: 0.932076
Ran for 36550 frames Cell Events: 1942 mean_circ: 0.932049
Ran for 37050 frames Cell Events: 1982 mean_circ: 0.932038
Ran for 38550 frames Cell Events: 2120 mean_circ: 0.931996
Ran for 39050 frames Cell Events: 2155 mean_circ: 0.931751
Ran for 40550 frames Cell Events: 2230 mean_circ: 0.931175
Ran for 41550 frames Cell Events: 2284 mean_circ: 0.931258
Ran for 42050 frames Cell Events: 2313 mean_circ: 0.93142
Ran for 42550 frames Cell Events: 2336 mean_circ: 0.931458
Ran for 43050 frames Cell Events: 2355 mean_circ: 0.931442
Ran for 43550 frames Cell Events: 2374 mean_circ: 0.931265
Ran for 44050 frames Cell Events: 2394 mean_circ: 0.931326
Ran for 44550 frames Cell Events: 2419 mean_circ: 0.931276
Ran for 45050 frames Cell Events: 2443 mean_circ: 0.931359
Ran for 45550 frames Cell Events: 2475 mean_circ: 0.931329
Ran for 46050 frames Cell Events: 2509 mean_circ: 0.931439
Ran for 46550 frames Cell Events: 2525 mean_circ: 0.931476
Ran for 47050 frames Cell Events: 2545 mean_circ: 0.931532
Ran for 48050 frames Cell Events: 2583 mean_circ: 0.931507
Ran for 48550 frames Cell Events: 2616 mean_circ: 0.931463
Ran for 49050 frames Cell Events: 2652 mean_circ: 0.931552
Ran for 49550 frames Cell Events: 2683 mean_circ: 0.931546
Ran for 50050 frames Cell Events: 2730 mean_circ: 0.931639
Ran for 51050 frames Cell Events: 2813 mean_circ: 0.931666
Ran for 52050 frames Cell Events: 2892 mean_circ: 0.931993
Ran for 53050 frames Cell Events: 2958 mean_circ: 0.931891
Ran for 54050 frames Cell Events: 2984 mean_circ: 0.931889
Ran for 56050 frames Cell Events: 3125 mean_circ: 0.931485
Ran for 56550 frames Cell Events: 3157 mean_circ: 0.931417
Ran for 57550 frames Cell Events: 3200 mean_circ: 0.931433
Ran for 58050 frames Cell Events: 3233 mean_circ: 0.93152
Ran for 58550 frames Cell Events: 3277 mean_circ: 0.931674
Ran for 60050 frames Cell Events: 3390 mean_circ: 0.931595
Ran for 60550 frames Cell Events: 3413 mean_circ: 0.931487
Ran for 62050 frames Cell Events: 3494 mean_circ: 0.931398
Ran for 62550 frames Cell Events: 3507 mean_circ: 0.931407
Ran for 63050 frames Cell Events: 3532 mean_circ: 0.931443
Ran for 63550 frames Cell Events: 3556 mean_circ: 0.931456
Ran for 64550 frames Cell Events: 3607 mean_circ: 0.931512
Ran for 65050 frames Cell Events: 3626 mean_circ: 0.931514
Ran for 65550 frames Cell Events: 3655 mean_circ: 0.931507
Ran for 67550 frames Cell Events: 3784 mean_circ: 0.931431
Ran for 68050 frames Cell Events: 3819 mean_circ: 0.93137
Ran for 68550 frames Cell Events: 3864 mean_circ: 0.931309
Ran for 69050 frames Cell Events: 3903 mean_circ: 0.931279
Ran for 69550 frames Cell Events: 3922 mean_circ: 0.931294
Ran for 70050 frames Cell Events: 3947 mean_circ: 0.931252
Ran for 71050 frames Cell Events: 4020 mean_circ: 0.931278
Ran for 71550 frames Cell Events: 4052 mean_circ: 0.931224
Ran for 72550 frames Cell Events: 4136 mean_circ: 0.931245
Ran for 73050 frames Cell Events: 4164 mean_circ: 0.931212
Ran for 74050 frames Cell Events: 4219 mean_circ: 0.931199
Ran for 74550 frames Cell Events: 4236 mean_circ: 0.931215
Ran for 75550 frames Cell Events: 4289 mean_circ: 0.931196
Ran for 76550 frames Cell Events: 4342 mean_circ: 0.931243
Ran for 77550 frames Cell Events: 4404 mean_circ: 0.931169
Ran for 79050 frames Cell Events: 4534 mean_circ: 0.931269
Ran for 79550 frames Cell Events: 4554 mean_circ: 0.931225
Ran for 80050 frames Cell Events: 4578 mean_circ: 0.9312
Ran for 81050 frames Cell Events: 4615 mean_circ: 0.931131
Ran for 81550 frames Cell Events: 4638 mean_circ: 0.931206
Ran for 82050 frames Cell Events: 4662 mean_circ: 0.93116
Ran for 82550 frames Cell Events: 4688 mean_circ: 0.931187
Ran for 83550 frames Cell Events: 4759 mean_circ: 0.931186
Ran for 84050 frames Cell Events: 4811 mean_circ: 0.931151
Ran for 86050 frames Cell Events: 4968 mean_circ: 0.931052
Ran for 87550 frames Cell Events: 5055 mean_circ: 0.931028
Ran for 89050 frames Cell Events: 5200 mean_circ: 0.930939
Ran for 89550 frames Cell Events: 5242 mean_circ: 0.931057
Ran for 91050 frames Cell Events: 5353 mean_circ: 0.930995
Ran for 92050 frames Cell Events: 5404 mean_circ: 0.931052
Ran for 93050 frames Cell Events: 5458 mean_circ: 0.931062
Ran for 93550 frames Cell Events: 5481 mean_circ: 0.931134
Ran for 94050 frames Cell Events: 5515 mean_circ: 0.931158
Ran for 94550 frames Cell Events: 5547 mean_circ: 0.931172
Ran for 95050 frames Cell Events: 5590 mean_circ: 0.93117
Ran for 96050 frames Cell Events: 5654 mean_circ: 0.93116
Ran for 96550 frames Cell Events: 5686 mean_circ: 0.931194
Ran for 97050 frames Cell Events: 5717 mean_circ: 0.931228
Ran for 97550 frames Cell Events: 5746 mean_circ: 0.9312
Ran for 98050 frames Cell Events: 5774 mean_circ: 0.931207
Ran for 101550 frames Cell Events: 6018 mean_circ: 0.93113
Ran for 102050 frames Cell Events: 6062 mean_circ: 0.931193
Ran for 102550 frames Cell Events: 6080 mean_circ: 0.931153
Ran for 103050 frames Cell Events: 6107 mean_circ: 0.9311
Ran for 103550 frames Cell Events: 6127 mean_circ: 0.931098
Ran for 104550 frames Cell Events: 6170 mean_circ: 0.931132
Ran for 105050 frames Cell Events: 6214 mean_circ: 0.931135
Ran for 105550 frames Cell Events: 6257 mean_circ: 0.931096
Ran for 106050 frames Cell Events: 6281 mean_circ: 0.931099
Ran for 106550 frames Cell Events: 6316 mean_circ: 0.93109
Ran for 107050 frames Cell Events: 6341 mean_circ: 0.931108
Ran for 107550 frames Cell Events: 6367 mean_circ: 0.931047
Ran for 109050 frames Cell Events: 6409 mean_circ: 0.93111
Ran for 109550 frames Cell Events: 6417 mean_circ: 0.931109
Ran for 110050 frames Cell Events: 6432 mean_circ: 0.931131
Ran for 110550 frames Cell Events: 6446 mean_circ: 0.931109
Ran for 111050 frames Cell Events: 6464 mean_circ: 0.931103
Ran for 112550 frames Cell Events: 6539 mean_circ: 0.931055
Ran for 113050 frames Cell Events: 6563 mean_circ: 0.931043
Ran for 114050 frames Cell Events: 6604 mean_circ: 0.931094
Ran for 115050 frames Cell Events: 6641 mean_circ: 0.931056
Ran for 115550 frames Cell Events: 6656 mean_circ: 0.931034
Ran for 116050 frames Cell Events: 6669 mean_circ: 0.931072
Ran for 116550 frames Cell Events: 6694 mean_circ: 0.931071
Ran for 118050 frames Cell Events: 6785 mean_circ: 0.930994
Ran for 118550 frames Cell Events: 6816 mean_circ: 0.930969
Ran for 119050 frames Cell Events: 6844 mean_circ: 0.931046
Ran for 119550 frames Cell Events: 6859 mean_circ: 0.931025
Ran for 121050 frames Cell Events: 6935 mean_circ: 0.930935
Ran for 122050 frames Cell Events: 7008 mean_circ: 0.930919
Ran for 122550 frames Cell Events: 7047 mean_circ: 0.930827
Ran for 123050 frames Cell Events: 7091 mean_circ: 0.930785
Ran for 123550 frames Cell Events: 7135 mean_circ: 0.930821
Ran for 124550 frames Cell Events: 7224 mean_circ: 0.930808
Ran for 125050 frames Cell Events: 7270 mean_circ: 0.930815
Ran for 125550 frames Cell Events: 7299 mean_circ: 0.930797
Ran for 126050 frames Cell Events: 7316 mean_circ: 0.93072
Ran for 126550 frames Cell Events: 7335 mean_circ: 0.930693
Ran for 127550 frames Cell Events: 7374 mean_circ: 0.930686
Ran for 128550 frames Cell Events: 7432 mean_circ: 0.930637
Ran for 129050 frames Cell Events: 7453 mean_circ: 0.93065
Ran for 129550 frames Cell Events: 7476 mean_circ: 0.930659
Ran for 130550 frames Cell Events: 7534 mean_circ: 0.930744
Ran for 131050 frames Cell Events: 7561 mean_circ: 0.930765
Ran for 131550 frames Cell Events: 7594 mean_circ: 0.930806
Ran for 132050 frames Cell Events: 7631 mean_circ: 0.930771
Ran for 135050 frames Cell Events: 7916 mean_circ: 0.930893
Ran for 135550 frames Cell Events: 7948 mean_circ: 0.930861
Ran for 136050 frames Cell Events: 7998 mean_circ: 0.930861
Ran for 136550 frames Cell Events: 8040 mean_circ: 0.930882
Ran for 137050 frames Cell Events: 8068 mean_circ: 0.930877
Ran for 138050 frames Cell Events: 8124 mean_circ: 0.930894
Ran for 138550 frames Cell Events: 8136 mean_circ: 0.930927
Ran for 139550 frames Cell Events: 8190 mean_circ: 0.93081
Ran for 141050 frames Cell Events: 8278 mean_circ: 0.930768
Ran for 141550 frames Cell Events: 8314 mean_circ: 0.930719
Ran for 142550 frames Cell Events: 8363 mean_circ: 0.930721
Ran for 143050 frames Cell Events: 8386 mean_circ: 0.930697
Ran for 143550 frames Cell Events: 8412 mean_circ: 0.930687
Ran for 144050 frames Cell Events: 8436 mean_circ: 0.930732
Ran for 144550 frames Cell Events: 8472 mean_circ: 0.930762
Ran for 145050 frames Cell Events: 8507 mean_circ: 0.930725
Ran for 146050 frames Cell Events: 8583 mean_circ: 0.930746
Ran for 146550 frames Cell Events: 8618 mean_circ: 0.930728
Ran for 147050 frames Cell Events: 8645 mean_circ: 0.930689
Ran for 147550 frames Cell Events: 8684 mean_circ: 0.930639
Ran for 148550 frames Cell Events: 8733 mean_circ: 0.930632
Ran for 149050 frames Cell Events: 8751 mean_circ: 0.930647
Ran for 149550 frames Cell Events: 8775 mean_circ: 0.930639
Ran for 152050 frames Cell Events: 8993 mean_circ: 0.930663
Ran for 152550 frames Cell Events: 9040 mean_circ: 0.930726
Ran for 154050 frames Cell Events: 9150 mean_circ: 0.930615
Ran for 156050 frames Cell Events: 9297 mean_circ: 0.930491
Ran for 156550 frames Cell Events: 9329 mean_circ: 0.930542
Ran for 157050 frames Cell Events: 9362 mean_circ: 0.930502
Ran for 157550 frames Cell Events: 9405 mean_circ: 0.930557
Ran for 158550 frames Cell Events: 9479 mean_circ: 0.930573
Ran for 159550 frames Cell Events: 9536 mean_circ: 0.930565
Ran for 160550 frames Cell Events: 9625 mean_circ: 0.930626
Ran for 161050 frames Cell Events: 9647 mean_circ: 0.930624
Ran for 161550 frames Cell Events: 9673 mean_circ: 0.930652
Ran for 162050 frames Cell Events: 9696 mean_circ: 0.930671
Ran for 162550 frames Cell Events: 9732 mean_circ: 0.930634
Ran for 163550 frames Cell Events: 9799 mean_circ: 0.930689
Ran for 164050 frames Cell Events: 9827 mean_circ: 0.930697
Ran for 165050 frames Cell Events: 9913 mean_circ: 0.9306
End this measurment.
because stop condition (time/nr of cells) was met
Stopping time: 4.84672e+008
****************************************
Measurement finished
Mean Circularitiy: 0.930503
Mean Area: 785.076
Mean FL-1 intensity: 11377.3
Mean FL-2 intensity: 13067
Mean FL-3 intensity: 0
frames processed: 164151
Total Time: 83074.4ms
Number of Runs (new bg img) 0
Frames collected: 165971
Skipped frames: 20195
Rescued Frames: 2866089
Samples DAQ: 41492805
Samples written: 82985526
Samples per Channel: 41492763
FPS: 1997.86
Samples per second: 499465
Expected samples/s: 500000
Events: 10000
Events per second: 120.374
jumped back (PMT): 16
#Frames read, frequency
# 0 frames :238399
# 1 frames :78125
# 2 frames :3543
# 3 frames :1247
# 4 frames :408
# 5 frames :3460
# 6 frames :1974
# 7 frames :1580
# 8 frames :707
# 9 frames :341
# 10 frames :333
# 11 frames :248
# 12 frames :338
# 13 frames :177
# 14 frames :125
# 15 frames :82
# 16 frames :63
# 17 frames :67
# 18 frames :52
# 19 frames :47
# 20 frames :41
# 21 frames :26
# 22 frames :28
# 23 frames :13
# 24 frames :14
# 25 frames :13
# 26 frames :10
# 27 frames :12
# 28 frames :10
# 29 frames :9
# 30 frames :4
# 31 frames :4
# 32 frames :3
# 33 frames :2
# 34 frames :5
# 35 frames :2
# 36 frames :4
# 37 frames :2
# 38 frames :1
# 39 frames :1
# 40 frames :2
# 42 frames :1
# 45 frames :2
# 47 frames :1
# 55 frames :1
# 56 frames :1
# 60 frames :2
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9751
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0