Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_19.29.54
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "5b6c43903f24c055fd3a8159194035c7",
"name": "M1_20170619_1801_dsRNA_25b_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:30:15"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8074 (max 8075) in '8074'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1204
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 4.85459e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 21 mean_circ: 0.924129
Ran for 1550 frames Cell Events: 56 mean_circ: 0.913798
Ran for 2050 frames Cell Events: 71 mean_circ: 0.913381
Ran for 2550 frames Cell Events: 85 mean_circ: 0.913578
Ran for 3050 frames Cell Events: 109 mean_circ: 0.912374
Ran for 4050 frames Cell Events: 169 mean_circ: 0.917066
Ran for 4550 frames Cell Events: 184 mean_circ: 0.916425
Ran for 5050 frames Cell Events: 199 mean_circ: 0.916994
Ran for 5550 frames Cell Events: 217 mean_circ: 0.918641
Ran for 6550 frames Cell Events: 251 mean_circ: 0.918475
Ran for 7050 frames Cell Events: 266 mean_circ: 0.917709
Ran for 7550 frames Cell Events: 281 mean_circ: 0.916829
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Ran for 8550 frames Cell Events: 320 mean_circ: 0.915884
Ran for 9050 frames Cell Events: 343 mean_circ: 0.915962
Ran for 10550 frames Cell Events: 390 mean_circ: 0.915857
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Ran for 12050 frames Cell Events: 466 mean_circ: 0.917334
Ran for 12550 frames Cell Events: 483 mean_circ: 0.918074
Ran for 13050 frames Cell Events: 497 mean_circ: 0.9178
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Ran for 33050 frames Cell Events: 1258 mean_circ: 0.917862
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Ran for 66550 frames Cell Events: 2349 mean_circ: 0.916557
Ran for 67050 frames Cell Events: 2361 mean_circ: 0.916411
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Ran for 73050 frames Cell Events: 2550 mean_circ: 0.916115
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Ran for 75550 frames Cell Events: 2641 mean_circ: 0.91644
Ran for 76550 frames Cell Events: 2705 mean_circ: 0.916445
Ran for 77550 frames Cell Events: 2741 mean_circ: 0.916535
Ran for 78050 frames Cell Events: 2755 mean_circ: 0.916478
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Ran for 79550 frames Cell Events: 2793 mean_circ: 0.916541
Ran for 80050 frames Cell Events: 2813 mean_circ: 0.916606
Ran for 80550 frames Cell Events: 2835 mean_circ: 0.916519
Ran for 81550 frames Cell Events: 2880 mean_circ: 0.916524
Ran for 82050 frames Cell Events: 2902 mean_circ: 0.916505
Ran for 82550 frames Cell Events: 2908 mean_circ: 0.91651
Ran for 83050 frames Cell Events: 2927 mean_circ: 0.916672
Ran for 83550 frames Cell Events: 2943 mean_circ: 0.916625
Ran for 84550 frames Cell Events: 2980 mean_circ: 0.916632
Ran for 85550 frames Cell Events: 3020 mean_circ: 0.916849
Ran for 86050 frames Cell Events: 3034 mean_circ: 0.916843
Ran for 86550 frames Cell Events: 3056 mean_circ: 0.91691
Ran for 87050 frames Cell Events: 3069 mean_circ: 0.916843
Ran for 88050 frames Cell Events: 3096 mean_circ: 0.916885
Ran for 91050 frames Cell Events: 3235 mean_circ: 0.917147
Ran for 91550 frames Cell Events: 3243 mean_circ: 0.917155
Ran for 93050 frames Cell Events: 3297 mean_circ: 0.917071
Ran for 93550 frames Cell Events: 3318 mean_circ: 0.917176
Ran for 94050 frames Cell Events: 3334 mean_circ: 0.917262
Ran for 94550 frames Cell Events: 3350 mean_circ: 0.917355
Ran for 95050 frames Cell Events: 3373 mean_circ: 0.917476
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Ran for 96550 frames Cell Events: 3421 mean_circ: 0.917352
Ran for 97050 frames Cell Events: 3432 mean_circ: 0.91737
Ran for 97550 frames Cell Events: 3450 mean_circ: 0.917306
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Ran for 100050 frames Cell Events: 3553 mean_circ: 0.917152
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Ran for 103050 frames Cell Events: 3642 mean_circ: 0.917312
Ran for 103550 frames Cell Events: 3663 mean_circ: 0.917382
Ran for 104050 frames Cell Events: 3681 mean_circ: 0.917352
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Ran for 106050 frames Cell Events: 3758 mean_circ: 0.917548
Ran for 106550 frames Cell Events: 3768 mean_circ: 0.917498
Ran for 107550 frames Cell Events: 3807 mean_circ: 0.917305
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Ran for 109050 frames Cell Events: 3884 mean_circ: 0.917373
Ran for 110550 frames Cell Events: 3937 mean_circ: 0.9173
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Ran for 112050 frames Cell Events: 3981 mean_circ: 0.917075
Ran for 112550 frames Cell Events: 3997 mean_circ: 0.917107
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Ran for 120050 frames Cell Events: 4290 mean_circ: 0.917115
Ran for 120550 frames Cell Events: 4304 mean_circ: 0.917066
Ran for 121550 frames Cell Events: 4343 mean_circ: 0.916982
Ran for 123050 frames Cell Events: 4398 mean_circ: 0.917096
Ran for 125550 frames Cell Events: 4490 mean_circ: 0.917005
Ran for 126550 frames Cell Events: 4526 mean_circ: 0.916884
Ran for 127050 frames Cell Events: 4541 mean_circ: 0.91688
Ran for 127550 frames Cell Events: 4557 mean_circ: 0.916908
Ran for 128550 frames Cell Events: 4582 mean_circ: 0.916848
Ran for 129050 frames Cell Events: 4597 mean_circ: 0.91679
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Ran for 131550 frames Cell Events: 4696 mean_circ: 0.916816
Ran for 133050 frames Cell Events: 4760 mean_circ: 0.916791
Ran for 133550 frames Cell Events: 4773 mean_circ: 0.916766
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Ran for 136050 frames Cell Events: 4907 mean_circ: 0.916646
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Ran for 138050 frames Cell Events: 4971 mean_circ: 0.916522
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Ran for 167050 frames Cell Events: 6013 mean_circ: 0.916048
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Ran for 182050 frames Cell Events: 6504 mean_circ: 0.916014
Ran for 183050 frames Cell Events: 6548 mean_circ: 0.916064
Ran for 183550 frames Cell Events: 6560 mean_circ: 0.916051
Ran for 184050 frames Cell Events: 6579 mean_circ: 0.916019
Ran for 184550 frames Cell Events: 6612 mean_circ: 0.916006
Ran for 185050 frames Cell Events: 6640 mean_circ: 0.916048
Ran for 186050 frames Cell Events: 6686 mean_circ: 0.916082
Ran for 186550 frames Cell Events: 6711 mean_circ: 0.916092
Ran for 188050 frames Cell Events: 6776 mean_circ: 0.916077
Ran for 189550 frames Cell Events: 6835 mean_circ: 0.916065
Ran for 190050 frames Cell Events: 6847 mean_circ: 0.916088
Ran for 190550 frames Cell Events: 6861 mean_circ: 0.916072
Ran for 191050 frames Cell Events: 6880 mean_circ: 0.916084
Ran for 191550 frames Cell Events: 6893 mean_circ: 0.916124
Ran for 192050 frames Cell Events: 6916 mean_circ: 0.916064
Ran for 192550 frames Cell Events: 6948 mean_circ: 0.916058
Ran for 193050 frames Cell Events: 6969 mean_circ: 0.916087
Ran for 193550 frames Cell Events: 6988 mean_circ: 0.916014
Ran for 194050 frames Cell Events: 6998 mean_circ: 0.915975
Ran for 194550 frames Cell Events: 7016 mean_circ: 0.91603
Ran for 195050 frames Cell Events: 7036 mean_circ: 0.916047
Ran for 195550 frames Cell Events: 7051 mean_circ: 0.916009
Ran for 196050 frames Cell Events: 7076 mean_circ: 0.916041
Ran for 196550 frames Cell Events: 7089 mean_circ: 0.916082
Ran for 197050 frames Cell Events: 7107 mean_circ: 0.916073
Ran for 198050 frames Cell Events: 7138 mean_circ: 0.916101
Ran for 198550 frames Cell Events: 7155 mean_circ: 0.916078
Ran for 199550 frames Cell Events: 7180 mean_circ: 0.91601
Ran for 200050 frames Cell Events: 7188 mean_circ: 0.916041
Ran for 200550 frames Cell Events: 7200 mean_circ: 0.916031
Ran for 201550 frames Cell Events: 7255 mean_circ: 0.916062
Ran for 202050 frames Cell Events: 7269 mean_circ: 0.916072
Ran for 203050 frames Cell Events: 7310 mean_circ: 0.916121
Ran for 203550 frames Cell Events: 7333 mean_circ: 0.916135
Ran for 204050 frames Cell Events: 7359 mean_circ: 0.916142
Ran for 204550 frames Cell Events: 7378 mean_circ: 0.916109
Ran for 205050 frames Cell Events: 7391 mean_circ: 0.916106
Ran for 205550 frames Cell Events: 7403 mean_circ: 0.916137
Ran for 206050 frames Cell Events: 7407 mean_circ: 0.916089
Ran for 206550 frames Cell Events: 7419 mean_circ: 0.916096
Ran for 207050 frames Cell Events: 7433 mean_circ: 0.916123
Ran for 207550 frames Cell Events: 7452 mean_circ: 0.916149
Ran for 209050 frames Cell Events: 7504 mean_circ: 0.916176
Ran for 210050 frames Cell Events: 7537 mean_circ: 0.916187
Ran for 210550 frames Cell Events: 7552 mean_circ: 0.916229
Ran for 211550 frames Cell Events: 7581 mean_circ: 0.916253
Ran for 212050 frames Cell Events: 7598 mean_circ: 0.916262
Ran for 212550 frames Cell Events: 7620 mean_circ: 0.916261
Ran for 213050 frames Cell Events: 7634 mean_circ: 0.916252
Ran for 214050 frames Cell Events: 7665 mean_circ: 0.916249
Ran for 214550 frames Cell Events: 7679 mean_circ: 0.916248
Ran for 215550 frames Cell Events: 7713 mean_circ: 0.91626
Ran for 216050 frames Cell Events: 7730 mean_circ: 0.916277
Ran for 216550 frames Cell Events: 7750 mean_circ: 0.916284
Ran for 217050 frames Cell Events: 7770 mean_circ: 0.916255
Ran for 217550 frames Cell Events: 7786 mean_circ: 0.916228
Ran for 218050 frames Cell Events: 7800 mean_circ: 0.916174
Ran for 218550 frames Cell Events: 7821 mean_circ: 0.916188
Ran for 219550 frames Cell Events: 7868 mean_circ: 0.916103
Ran for 220550 frames Cell Events: 7916 mean_circ: 0.915937
Ran for 221050 frames Cell Events: 7934 mean_circ: 0.915926
Ran for 222550 frames Cell Events: 7997 mean_circ: 0.915923
Ran for 223050 frames Cell Events: 8018 mean_circ: 0.915871
Ran for 223550 frames Cell Events: 8034 mean_circ: 0.915823
Ran for 224050 frames Cell Events: 8048 mean_circ: 0.915837
Ran for 224550 frames Cell Events: 8066 mean_circ: 0.915857
End this measurment..
Stopping time: 4.85572e+008
****************************************
Measurement finished
Mean Circularitiy: 0.915856
Mean Area: 676.984
Mean FL-1 intensity: 2675.61
Mean FL-2 intensity: 3829.79
Mean FL-3 intensity: 0
frames processed: 224676
Total Time: 112551ms
Number of Runs (new bg img) 0
Frames collected: 224889
Skipped frames: 20663
Rescued Frames: 3044279
Samples DAQ: 56222744
Samples written: 112444666
Samples per Channel: 56222333
FPS: 1998.1
Samples per second: 499527
Expected samples/s: 500000
Events: 8075
Events per second: 71.7451
jumped back (PMT): 14
#Frames read, frequency
# 0 frames :310081
# 1 frames :122119
# 2 frames :5285
# 3 frames :1866
# 4 frames :850
# 5 frames :1794
# 6 frames :2091
# 7 frames :1161
# 8 frames :613
# 9 frames :440
# 10 frames :1063
# 11 frames :635
# 12 frames :450
# 13 frames :275
# 14 frames :236
# 15 frames :194
# 16 frames :114
# 17 frames :90
# 18 frames :72
# 19 frames :59
# 20 frames :40
# 21 frames :47
# 22 frames :17
# 23 frames :18
# 24 frames :14
# 25 frames :22
# 26 frames :17
# 27 frames :19
# 28 frames :13
# 29 frames :9
# 30 frames :12
# 31 frames :4
# 32 frames :2
# 33 frames :1
# 37 frames :1
# 38 frames :1
# 39 frames :1
# 41 frames :1
# 42 frames :2
# 46 frames :1
# 68 frames :1
# 71 frames :1
# 74 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1204
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0