Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_19.32.14
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "4d5c8c23035ab458dc90661b7055f802",
"name": "M1_20170620_1351_dsRNA_T1_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:32:35"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8326 (max 8327) in '8326'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.4062
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 5.56813e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 17 mean_circ: 0.928871
Ran for 1050 frames Cell Events: 33 mean_circ: 0.95405
Ran for 1550 frames Cell Events: 42 mean_circ: 0.956939
Ran for 2550 frames Cell Events: 66 mean_circ: 0.955428
Ran for 3050 frames Cell Events: 86 mean_circ: 0.95686
Ran for 3550 frames Cell Events: 96 mean_circ: 0.956161
Ran for 4050 frames Cell Events: 113 mean_circ: 0.956831
Ran for 4550 frames Cell Events: 140 mean_circ: 0.95746
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Ran for 6050 frames Cell Events: 194 mean_circ: 0.955143
Ran for 6550 frames Cell Events: 206 mean_circ: 0.955018
Ran for 7550 frames Cell Events: 253 mean_circ: 0.955706
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Ran for 9550 frames Cell Events: 311 mean_circ: 0.954617
Ran for 10050 frames Cell Events: 321 mean_circ: 0.95474
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Ran for 83050 frames Cell Events: 2642 mean_circ: 0.950551
Ran for 84050 frames Cell Events: 2686 mean_circ: 0.950536
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Ran for 85550 frames Cell Events: 2735 mean_circ: 0.950604
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Ran for 91550 frames Cell Events: 2951 mean_circ: 0.950776
Ran for 92050 frames Cell Events: 2961 mean_circ: 0.95077
Ran for 92550 frames Cell Events: 2982 mean_circ: 0.950798
Ran for 93050 frames Cell Events: 2990 mean_circ: 0.950829
Ran for 93550 frames Cell Events: 3006 mean_circ: 0.950865
Ran for 94050 frames Cell Events: 3024 mean_circ: 0.950805
Ran for 95050 frames Cell Events: 3066 mean_circ: 0.950848
Ran for 95550 frames Cell Events: 3087 mean_circ: 0.9508
Ran for 96050 frames Cell Events: 3101 mean_circ: 0.950843
Ran for 96550 frames Cell Events: 3115 mean_circ: 0.950821
Ran for 97550 frames Cell Events: 3147 mean_circ: 0.950871
Ran for 98050 frames Cell Events: 3158 mean_circ: 0.950865
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Ran for 99550 frames Cell Events: 3212 mean_circ: 0.950906
Ran for 100550 frames Cell Events: 3243 mean_circ: 0.950946
Ran for 101050 frames Cell Events: 3258 mean_circ: 0.950962
Ran for 101550 frames Cell Events: 3272 mean_circ: 0.950923
Ran for 102050 frames Cell Events: 3283 mean_circ: 0.950925
Ran for 103550 frames Cell Events: 3335 mean_circ: 0.951014
Ran for 104050 frames Cell Events: 3353 mean_circ: 0.951037
Ran for 105550 frames Cell Events: 3397 mean_circ: 0.950832
Ran for 107050 frames Cell Events: 3442 mean_circ: 0.95093
Ran for 107550 frames Cell Events: 3466 mean_circ: 0.950913
Ran for 108050 frames Cell Events: 3489 mean_circ: 0.950903
Ran for 108550 frames Cell Events: 3504 mean_circ: 0.950869
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Ran for 122550 frames Cell Events: 3988 mean_circ: 0.950936
Ran for 123550 frames Cell Events: 4023 mean_circ: 0.950914
Ran for 124050 frames Cell Events: 4046 mean_circ: 0.950971
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Ran for 126050 frames Cell Events: 4118 mean_circ: 0.951044
Ran for 126550 frames Cell Events: 4136 mean_circ: 0.951008
Ran for 127550 frames Cell Events: 4171 mean_circ: 0.950932
Ran for 128050 frames Cell Events: 4195 mean_circ: 0.950872
Ran for 129050 frames Cell Events: 4221 mean_circ: 0.95088
Ran for 131550 frames Cell Events: 4296 mean_circ: 0.950922
Ran for 132050 frames Cell Events: 4315 mean_circ: 0.95089
Ran for 132550 frames Cell Events: 4328 mean_circ: 0.950887
Ran for 133050 frames Cell Events: 4340 mean_circ: 0.9509
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Ran for 141550 frames Cell Events: 4640 mean_circ: 0.950978
Ran for 145050 frames Cell Events: 4776 mean_circ: 0.950954
Ran for 145550 frames Cell Events: 4800 mean_circ: 0.950926
Ran for 146550 frames Cell Events: 4830 mean_circ: 0.950921
Ran for 147550 frames Cell Events: 4865 mean_circ: 0.950855
Ran for 149050 frames Cell Events: 4915 mean_circ: 0.950836
Ran for 149550 frames Cell Events: 4928 mean_circ: 0.950847
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Ran for 151050 frames Cell Events: 4979 mean_circ: 0.950813
Ran for 151550 frames Cell Events: 4998 mean_circ: 0.950802
Ran for 152050 frames Cell Events: 5012 mean_circ: 0.950775
Ran for 152550 frames Cell Events: 5027 mean_circ: 0.950722
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Ran for 156050 frames Cell Events: 5155 mean_circ: 0.950677
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Ran for 157050 frames Cell Events: 5179 mean_circ: 0.950716
Ran for 158050 frames Cell Events: 5220 mean_circ: 0.950765
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Ran for 207050 frames Cell Events: 7007 mean_circ: 0.950468
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Ran for 210050 frames Cell Events: 7123 mean_circ: 0.950484
Ran for 210550 frames Cell Events: 7135 mean_circ: 0.950495
Ran for 211050 frames Cell Events: 7144 mean_circ: 0.95046
Ran for 211550 frames Cell Events: 7158 mean_circ: 0.950455
Ran for 212050 frames Cell Events: 7176 mean_circ: 0.950474
Ran for 212550 frames Cell Events: 7185 mean_circ: 0.950471
Ran for 213050 frames Cell Events: 7203 mean_circ: 0.950482
Ran for 213550 frames Cell Events: 7219 mean_circ: 0.950448
Ran for 215050 frames Cell Events: 7268 mean_circ: 0.95038
Ran for 215550 frames Cell Events: 7282 mean_circ: 0.950377
Ran for 216550 frames Cell Events: 7315 mean_circ: 0.950351
Ran for 217050 frames Cell Events: 7332 mean_circ: 0.950372
Ran for 217550 frames Cell Events: 7344 mean_circ: 0.950373
Ran for 218050 frames Cell Events: 7363 mean_circ: 0.950392
Ran for 218550 frames Cell Events: 7384 mean_circ: 0.950389
Ran for 219550 frames Cell Events: 7420 mean_circ: 0.950391
Ran for 220050 frames Cell Events: 7439 mean_circ: 0.950373
Ran for 221050 frames Cell Events: 7469 mean_circ: 0.950385
Ran for 221550 frames Cell Events: 7479 mean_circ: 0.950384
Ran for 222050 frames Cell Events: 7499 mean_circ: 0.950365
Ran for 222550 frames Cell Events: 7520 mean_circ: 0.950351
Ran for 223050 frames Cell Events: 7542 mean_circ: 0.95033
Ran for 224050 frames Cell Events: 7580 mean_circ: 0.950369
Ran for 225050 frames Cell Events: 7614 mean_circ: 0.950354
Ran for 226050 frames Cell Events: 7655 mean_circ: 0.950312
Ran for 226550 frames Cell Events: 7672 mean_circ: 0.950316
Ran for 227550 frames Cell Events: 7705 mean_circ: 0.950332
Ran for 228050 frames Cell Events: 7723 mean_circ: 0.950312
Ran for 228550 frames Cell Events: 7735 mean_circ: 0.950312
Ran for 229050 frames Cell Events: 7747 mean_circ: 0.950283
Ran for 229550 frames Cell Events: 7770 mean_circ: 0.950312
Ran for 230050 frames Cell Events: 7785 mean_circ: 0.950321
Ran for 231050 frames Cell Events: 7822 mean_circ: 0.95034
Ran for 232050 frames Cell Events: 7854 mean_circ: 0.950377
Ran for 232550 frames Cell Events: 7867 mean_circ: 0.950353
Ran for 233550 frames Cell Events: 7903 mean_circ: 0.950352
Ran for 234050 frames Cell Events: 7921 mean_circ: 0.950356
Ran for 234550 frames Cell Events: 7941 mean_circ: 0.950356
Ran for 235050 frames Cell Events: 7965 mean_circ: 0.950352
Ran for 236050 frames Cell Events: 8004 mean_circ: 0.950385
Ran for 236550 frames Cell Events: 8015 mean_circ: 0.950418
Ran for 237050 frames Cell Events: 8030 mean_circ: 0.950428
Ran for 237550 frames Cell Events: 8052 mean_circ: 0.950425
Ran for 239550 frames Cell Events: 8127 mean_circ: 0.95044
Ran for 240050 frames Cell Events: 8141 mean_circ: 0.950418
Ran for 240550 frames Cell Events: 8169 mean_circ: 0.950437
Ran for 241050 frames Cell Events: 8183 mean_circ: 0.95042
Ran for 241550 frames Cell Events: 8197 mean_circ: 0.950428
Ran for 242050 frames Cell Events: 8217 mean_circ: 0.950379
Ran for 242550 frames Cell Events: 8231 mean_circ: 0.950386
Ran for 243550 frames Cell Events: 8273 mean_circ: 0.95037
Ran for 244550 frames Cell Events: 8316 mean_circ: 0.95038
End this measurment..
Stopping time: 5.56935e+008
****************************************
Measurement finished
Mean Circularitiy: 0.950377
Mean Area: 613.021
Mean FL-1 intensity: 3853.48
Mean FL-2 intensity: 4553.48
Mean FL-3 intensity: 0
frames processed: 244784
Total Time: 122540ms
Number of Runs (new bg img) 0
Frames collected: 244918
Skipped frames: 2175
Rescued Frames: 553378
Samples DAQ: 61229614
Samples written: 122459032
Samples per Channel: 61229516
FPS: 1998.68
Samples per second: 499669
Expected samples/s: 500000
Events: 8327
Events per second: 67.9533
jumped back (PMT): 5187
#Frames read, frequency
# 0 frames :352423
# 1 frames :118541
# 2 frames :5523
# 3 frames :1896
# 4 frames :1275
# 5 frames :568
# 6 frames :1337
# 7 frames :2006
# 8 frames :1146
# 9 frames :725
# 10 frames :722
# 11 frames :707
# 12 frames :555
# 13 frames :411
# 14 frames :315
# 15 frames :295
# 16 frames :230
# 17 frames :261
# 18 frames :172
# 19 frames :121
# 20 frames :85
# 21 frames :80
# 22 frames :62
# 23 frames :51
# 24 frames :49
# 25 frames :47
# 26 frames :29
# 27 frames :33
# 28 frames :32
# 29 frames :21
# 30 frames :20
# 31 frames :15
# 32 frames :11
# 33 frames :11
# 34 frames :4
# 35 frames :4
# 36 frames :6
# 37 frames :4
# 38 frames :1
# 39 frames :3
# 40 frames :1
# 41 frames :4
# 42 frames :1
# 43 frames :1
# 44 frames :1
# 45 frames :1
# 47 frames :2
# 51 frames :1
# 83 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.4062
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0