Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_19.33.40
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "7cc33f0701d9dac2d7a9a1e7599fda0a",
"name": "M1_20170620_1407_dsRNA_T7_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:34:01"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8118 (max 8119) in '8118'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.4795
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 5.57769e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 15 mean_circ: 0.946285
Ran for 1050 frames Cell Events: 26 mean_circ: 0.948181
Ran for 4050 frames Cell Events: 126 mean_circ: 0.935931
Ran for 5050 frames Cell Events: 158 mean_circ: 0.935521
Ran for 5550 frames Cell Events: 176 mean_circ: 0.935319
Ran for 6050 frames Cell Events: 191 mean_circ: 0.9343
Ran for 6550 frames Cell Events: 209 mean_circ: 0.934138
Ran for 7050 frames Cell Events: 227 mean_circ: 0.934943
Ran for 8550 frames Cell Events: 266 mean_circ: 0.932302
Ran for 9550 frames Cell Events: 291 mean_circ: 0.931081
Ran for 10050 frames Cell Events: 305 mean_circ: 0.931102
Ran for 10550 frames Cell Events: 324 mean_circ: 0.930651
Ran for 11050 frames Cell Events: 341 mean_circ: 0.930154
Ran for 11550 frames Cell Events: 355 mean_circ: 0.929403
Ran for 12050 frames Cell Events: 376 mean_circ: 0.929111
Ran for 12550 frames Cell Events: 389 mean_circ: 0.929147
Ran for 14050 frames Cell Events: 460 mean_circ: 0.930256
Ran for 15050 frames Cell Events: 494 mean_circ: 0.930265
Ran for 15550 frames Cell Events: 513 mean_circ: 0.929892
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Ran for 18550 frames Cell Events: 643 mean_circ: 0.928391
Ran for 19050 frames Cell Events: 652 mean_circ: 0.928315
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Ran for 20050 frames Cell Events: 692 mean_circ: 0.928442
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Ran for 73050 frames Cell Events: 2622 mean_circ: 0.926203
Ran for 73550 frames Cell Events: 2641 mean_circ: 0.926309
Ran for 74550 frames Cell Events: 2673 mean_circ: 0.926379
Ran for 75550 frames Cell Events: 2706 mean_circ: 0.926249
Ran for 76550 frames Cell Events: 2752 mean_circ: 0.92605
Ran for 77050 frames Cell Events: 2769 mean_circ: 0.926084
Ran for 77550 frames Cell Events: 2787 mean_circ: 0.925948
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Ran for 80550 frames Cell Events: 2882 mean_circ: 0.925862
Ran for 81050 frames Cell Events: 2895 mean_circ: 0.925904
Ran for 81550 frames Cell Events: 2906 mean_circ: 0.925972
Ran for 83050 frames Cell Events: 2956 mean_circ: 0.925872
Ran for 83550 frames Cell Events: 2978 mean_circ: 0.92584
Ran for 84050 frames Cell Events: 3000 mean_circ: 0.925791
Ran for 84550 frames Cell Events: 3021 mean_circ: 0.925836
Ran for 85050 frames Cell Events: 3034 mean_circ: 0.925809
Ran for 85550 frames Cell Events: 3048 mean_circ: 0.92579
Ran for 86550 frames Cell Events: 3088 mean_circ: 0.925689
Ran for 88050 frames Cell Events: 3142 mean_circ: 0.925758
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Ran for 91050 frames Cell Events: 3247 mean_circ: 0.925716
Ran for 91550 frames Cell Events: 3265 mean_circ: 0.925729
Ran for 92050 frames Cell Events: 3279 mean_circ: 0.925757
Ran for 92550 frames Cell Events: 3294 mean_circ: 0.925693
Ran for 93050 frames Cell Events: 3315 mean_circ: 0.925756
Ran for 93550 frames Cell Events: 3330 mean_circ: 0.925791
Ran for 94550 frames Cell Events: 3372 mean_circ: 0.925846
Ran for 95050 frames Cell Events: 3383 mean_circ: 0.925927
Ran for 95550 frames Cell Events: 3397 mean_circ: 0.925904
Ran for 96050 frames Cell Events: 3409 mean_circ: 0.925939
Ran for 96550 frames Cell Events: 3418 mean_circ: 0.925806
Ran for 97050 frames Cell Events: 3427 mean_circ: 0.925745
Ran for 98050 frames Cell Events: 3457 mean_circ: 0.925847
Ran for 98550 frames Cell Events: 3476 mean_circ: 0.925828
Ran for 101050 frames Cell Events: 3582 mean_circ: 0.92597
Ran for 101550 frames Cell Events: 3601 mean_circ: 0.925964
Ran for 102050 frames Cell Events: 3616 mean_circ: 0.925875
Ran for 102550 frames Cell Events: 3632 mean_circ: 0.925903
Ran for 103050 frames Cell Events: 3647 mean_circ: 0.925909
Ran for 104050 frames Cell Events: 3693 mean_circ: 0.925974
Ran for 105050 frames Cell Events: 3720 mean_circ: 0.925926
Ran for 105550 frames Cell Events: 3740 mean_circ: 0.925951
Ran for 106550 frames Cell Events: 3785 mean_circ: 0.925925
Ran for 107050 frames Cell Events: 3804 mean_circ: 0.925883
Ran for 107550 frames Cell Events: 3819 mean_circ: 0.925926
Ran for 108050 frames Cell Events: 3827 mean_circ: 0.925891
Ran for 108550 frames Cell Events: 3845 mean_circ: 0.925842
Ran for 109050 frames Cell Events: 3863 mean_circ: 0.925854
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Ran for 122550 frames Cell Events: 4354 mean_circ: 0.926149
Ran for 123050 frames Cell Events: 4371 mean_circ: 0.926172
Ran for 123550 frames Cell Events: 4398 mean_circ: 0.926204
Ran for 124050 frames Cell Events: 4418 mean_circ: 0.926256
Ran for 125050 frames Cell Events: 4451 mean_circ: 0.926339
Ran for 125550 frames Cell Events: 4460 mean_circ: 0.926346
Ran for 126050 frames Cell Events: 4472 mean_circ: 0.926299
Ran for 127050 frames Cell Events: 4503 mean_circ: 0.926358
Ran for 127550 frames Cell Events: 4518 mean_circ: 0.926332
Ran for 128050 frames Cell Events: 4542 mean_circ: 0.92631
Ran for 129050 frames Cell Events: 4578 mean_circ: 0.926303
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Ran for 131050 frames Cell Events: 4653 mean_circ: 0.926324
Ran for 132550 frames Cell Events: 4695 mean_circ: 0.926197
Ran for 133050 frames Cell Events: 4716 mean_circ: 0.926177
Ran for 133550 frames Cell Events: 4732 mean_circ: 0.926189
Ran for 134050 frames Cell Events: 4752 mean_circ: 0.926152
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Ran for 136050 frames Cell Events: 4832 mean_circ: 0.926102
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Ran for 138050 frames Cell Events: 4906 mean_circ: 0.926118
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Ran for 140050 frames Cell Events: 4976 mean_circ: 0.926091
Ran for 141050 frames Cell Events: 5010 mean_circ: 0.926053
Ran for 141550 frames Cell Events: 5022 mean_circ: 0.926031
Ran for 142050 frames Cell Events: 5039 mean_circ: 0.926007
Ran for 144550 frames Cell Events: 5152 mean_circ: 0.925948
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Ran for 151050 frames Cell Events: 5391 mean_circ: 0.925954
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Ran for 152050 frames Cell Events: 5431 mean_circ: 0.925909
Ran for 152550 frames Cell Events: 5443 mean_circ: 0.925957
Ran for 153050 frames Cell Events: 5457 mean_circ: 0.925985
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Ran for 204550 frames Cell Events: 7344 mean_circ: 0.925252
Ran for 205050 frames Cell Events: 7363 mean_circ: 0.925256
Ran for 205550 frames Cell Events: 7377 mean_circ: 0.925238
Ran for 206550 frames Cell Events: 7415 mean_circ: 0.925275
Ran for 207550 frames Cell Events: 7448 mean_circ: 0.925262
Ran for 208050 frames Cell Events: 7463 mean_circ: 0.925244
Ran for 208550 frames Cell Events: 7476 mean_circ: 0.925227
Ran for 210550 frames Cell Events: 7528 mean_circ: 0.925222
Ran for 211050 frames Cell Events: 7544 mean_circ: 0.925261
Ran for 211550 frames Cell Events: 7555 mean_circ: 0.925239
Ran for 213050 frames Cell Events: 7613 mean_circ: 0.925219
Ran for 213550 frames Cell Events: 7630 mean_circ: 0.925221
Ran for 214050 frames Cell Events: 7639 mean_circ: 0.925204
Ran for 214550 frames Cell Events: 7647 mean_circ: 0.92521
Ran for 215050 frames Cell Events: 7669 mean_circ: 0.925228
Ran for 215550 frames Cell Events: 7682 mean_circ: 0.925249
Ran for 216050 frames Cell Events: 7706 mean_circ: 0.925232
Ran for 216550 frames Cell Events: 7729 mean_circ: 0.925182
Ran for 217050 frames Cell Events: 7753 mean_circ: 0.925179
Ran for 217550 frames Cell Events: 7770 mean_circ: 0.925166
Ran for 218550 frames Cell Events: 7820 mean_circ: 0.925092
Ran for 220050 frames Cell Events: 7880 mean_circ: 0.925071
Ran for 220550 frames Cell Events: 7898 mean_circ: 0.92504
Ran for 221050 frames Cell Events: 7917 mean_circ: 0.925055
Ran for 221550 frames Cell Events: 7927 mean_circ: 0.925009
Ran for 222550 frames Cell Events: 7962 mean_circ: 0.925021
Ran for 223050 frames Cell Events: 7981 mean_circ: 0.92501
Ran for 224050 frames Cell Events: 8009 mean_circ: 0.925049
Ran for 225050 frames Cell Events: 8053 mean_circ: 0.924974
Ran for 225550 frames Cell Events: 8071 mean_circ: 0.924929
Ran for 226050 frames Cell Events: 8084 mean_circ: 0.924953
End this measRan for 227050 frames Cell Events: 8119 mean_circ: u0.924941
ment..
Stopping time: 5.57883e+008
****************************************
Measurement finished
Mean Circularitiy: 0.924937
Mean Area: 679.755
Mean FL-1 intensity: 3588.99
Mean FL-2 intensity: 4883.3
Mean FL-3 intensity: 0
frames processed: 227050
Total Time: 113586ms
Number of Runs (new bg img) 0
Frames collected: 227050
Skipped frames: 2524
Rescued Frames: 856578
Samples DAQ: 56763404
Samples written: 113524794
Samples per Channel: 56762397
FPS: 1998.92
Samples per second: 499729
Expected samples/s: 500000
Events: 8119
Events per second: 71.4787
jumped back (PMT): 19
#Frames read, frequency
# 0 frames :321594
# 1 frames :113848
# 2 frames :5710
# 3 frames :1978
# 4 frames :1118
# 5 frames :486
# 6 frames :1891
# 7 frames :2017
# 8 frames :999
# 9 frames :680
# 10 frames :1220
# 11 frames :598
# 12 frames :477
# 13 frames :274
# 14 frames :206
# 15 frames :218
# 16 frames :184
# 17 frames :141
# 18 frames :100
# 19 frames :63
# 20 frames :41
# 21 frames :38
# 22 frames :32
# 23 frames :30
# 24 frames :38
# 25 frames :19
# 26 frames :15
# 27 frames :11
# 28 frames :9
# 29 frames :6
# 30 frames :6
# 31 frames :9
# 32 frames :2
# 33 frames :7
# 34 frames :2
# 35 frames :3
# 37 frames :2
# 40 frames :4
# 41 frames :1
# 49 frames :1
# 50 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.4795
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0