Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_19.42.30
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "54567b2b819b7222a0bc4d5fd655c08b",
"name": "M1_20170620_1607_dsRNA_W3_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:42:51"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8095 (max 8096) in '8095'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9143
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 5.65022e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 24 mean_circ: 0.925253
Ran for 1550 frames Cell Events: 58 mean_circ: 0.926752
Ran for 2550 frames Cell Events: 89 mean_circ: 0.933815
Ran for 3050 frames Cell Events: 116 mean_circ: 0.932738
Ran for 4050 frames Cell Events: 136 mean_circ: 0.93252
Ran for 5050 frames Cell Events: 181 mean_circ: 0.934198
Ran for 5550 frames Cell Events: 206 mean_circ: 0.933477
Ran for 6050 frames Cell Events: 222 mean_circ: 0.933858
Ran for 6550 frames Cell Events: 232 mean_circ: 0.934206
Ran for 7050 frames Cell Events: 255 mean_circ: 0.933496
Ran for 7550 frames Cell Events: 268 mean_circ: 0.933855
Ran for 8050 frames Cell Events: 284 mean_circ: 0.933418
Ran for 8550 frames Cell Events: 298 mean_circ: 0.932898
Ran for 9550 frames Cell Events: 338 mean_circ: 0.930806
Ran for 10050 frames Cell Events: 353 mean_circ: 0.931744
Ran for 10550 frames Cell Events: 382 mean_circ: 0.932672
Ran for 11050 frames Cell Events: 402 mean_circ: 0.932626
Ran for 11550 frames Cell Events: 430 mean_circ: 0.932626
Ran for 14050 frames Cell Events: 497 mean_circ: 0.933479
Ran for 14550 frames Cell Events: 519 mean_circ: 0.93384
Ran for 15050 frames Cell Events: 535 mean_circ: 0.933593
Ran for 17550 frames Cell Events: 629 mean_circ: 0.933996
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Ran for 19050 frames Cell Events: 681 mean_circ: 0.934194
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Ran for 24550 frames Cell Events: 874 mean_circ: 0.933664
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Ran for 35550 frames Cell Events: 1283 mean_circ: 0.9336
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Ran for 40050 frames Cell Events: 1468 mean_circ: 0.933626
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Ran for 45550 frames Cell Events: 1666 mean_circ: 0.933942
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Ran for 47050 frames Cell Events: 1708 mean_circ: 0.934142
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Ran for 52050 frames Cell Events: 1875 mean_circ: 0.933725
Ran for 52550 frames Cell Events: 1890 mean_circ: 0.93371
Ran for 53550 frames Cell Events: 1925 mean_circ: 0.933737
Ran for 54550 frames Cell Events: 1956 mean_circ: 0.933836
Ran for 55050 frames Cell Events: 1972 mean_circ: 0.933878
Ran for 57050 frames Cell Events: 2050 mean_circ: 0.934081
Ran for 58050 frames Cell Events: 2082 mean_circ: 0.934223
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Ran for 59550 frames Cell Events: 2144 mean_circ: 0.934396
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Ran for 65050 frames Cell Events: 2338 mean_circ: 0.933868
Ran for 65550 frames Cell Events: 2357 mean_circ: 0.933773
Ran for 66050 frames Cell Events: 2369 mean_circ: 0.933859
Ran for 66550 frames Cell Events: 2391 mean_circ: 0.933946
Ran for 67050 frames Cell Events: 2403 mean_circ: 0.93399
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Ran for 68050 frames Cell Events: 2453 mean_circ: 0.933891
Ran for 69050 frames Cell Events: 2489 mean_circ: 0.933682
Ran for 70550 frames Cell Events: 2527 mean_circ: 0.933512
Ran for 71550 frames Cell Events: 2572 mean_circ: 0.933526
Ran for 73050 frames Cell Events: 2625 mean_circ: 0.933652
Ran for 74050 frames Cell Events: 2672 mean_circ: 0.933555
Ran for 74550 frames Cell Events: 2685 mean_circ: 0.933593
Ran for 75050 frames Cell Events: 2697 mean_circ: 0.93356
Ran for 75550 frames Cell Events: 2723 mean_circ: 0.933577
Ran for 76050 frames Cell Events: 2745 mean_circ: 0.933536
Ran for 78050 frames Cell Events: 2837 mean_circ: 0.93344
Ran for 78550 frames Cell Events: 2864 mean_circ: 0.933536
Ran for 79050 frames Cell Events: 2879 mean_circ: 0.933559
Ran for 80050 frames Cell Events: 2917 mean_circ: 0.933685
Ran for 81050 frames Cell Events: 2960 mean_circ: 0.933676
Ran for 81550 frames Cell Events: 2980 mean_circ: 0.933601
Ran for 82050 frames Cell Events: 2999 mean_circ: 0.933582
Ran for 82550 frames Cell Events: 3009 mean_circ: 0.933598
Ran for 83050 frames Cell Events: 3032 mean_circ: 0.933589
Ran for 83550 frames Cell Events: 3055 mean_circ: 0.933585
Ran for 84550 frames Cell Events: 3105 mean_circ: 0.933554
Ran for 85550 frames Cell Events: 3148 mean_circ: 0.933439
Ran for 87550 frames Cell Events: 3237 mean_circ: 0.933574
Ran for 88050 frames Cell Events: 3252 mean_circ: 0.933606
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Ran for 89550 frames Cell Events: 3308 mean_circ: 0.933592
Ran for 90050 frames Cell Events: 3326 mean_circ: 0.933701
Ran for 90550 frames Cell Events: 3346 mean_circ: 0.933766
Ran for 91050 frames Cell Events: 3361 mean_circ: 0.933863
Ran for 91550 frames Cell Events: 3374 mean_circ: 0.933878
Ran for 92550 frames Cell Events: 3403 mean_circ: 0.933868
Ran for 93050 frames Cell Events: 3422 mean_circ: 0.933859
Ran for 93550 frames Cell Events: 3444 mean_circ: 0.933847
Ran for 94050 frames Cell Events: 3466 mean_circ: 0.933861
Ran for 95550 frames Cell Events: 3529 mean_circ: 0.933891
Ran for 96050 frames Cell Events: 3555 mean_circ: 0.933845
Ran for 96550 frames Cell Events: 3575 mean_circ: 0.933877
Ran for 97050 frames Cell Events: 3589 mean_circ: 0.933801
Ran for 98050 frames Cell Events: 3625 mean_circ: 0.933827
Ran for 98550 frames Cell Events: 3653 mean_circ: 0.933815
Ran for 99050 frames Cell Events: 3670 mean_circ: 0.933837
Ran for 100550 frames Cell Events: 3758 mean_circ: 0.933755
Ran for 101050 frames Cell Events: 3779 mean_circ: 0.933739
Ran for 101550 frames Cell Events: 3796 mean_circ: 0.933703
Ran for 102050 frames Cell Events: 3813 mean_circ: 0.933711
Ran for 102550 frames Cell Events: 3823 mean_circ: 0.933709
Ran for 103050 frames Cell Events: 3839 mean_circ: 0.933624
Ran for 104550 frames Cell Events: 3887 mean_circ: 0.933515
Ran for 105050 frames Cell Events: 3903 mean_circ: 0.933525
Ran for 105550 frames Cell Events: 3924 mean_circ: 0.933493
Ran for 106050 frames Cell Events: 3941 mean_circ: 0.933475
Ran for 106550 frames Cell Events: 3960 mean_circ: 0.933517
Ran for 107550 frames Cell Events: 4009 mean_circ: 0.933308
Ran for 110050 frames Cell Events: 4110 mean_circ: 0.933308
Ran for 110550 frames Cell Events: 4135 mean_circ: 0.933268
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Ran for 112550 frames Cell Events: 4231 mean_circ: 0.933213
Ran for 113050 frames Cell Events: 4253 mean_circ: 0.93325
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Ran for 120050 frames Cell Events: 4549 mean_circ: 0.933354
Ran for 121050 frames Cell Events: 4592 mean_circ: 0.93348
Ran for 122550 frames Cell Events: 4646 mean_circ: 0.933467
Ran for 123050 frames Cell Events: 4660 mean_circ: 0.933549
Ran for 123550 frames Cell Events: 4683 mean_circ: 0.933588
Ran for 124050 frames Cell Events: 4706 mean_circ: 0.933656
Ran for 125050 frames Cell Events: 4748 mean_circ: 0.933703
Ran for 126550 frames Cell Events: 4803 mean_circ: 0.933714
Ran for 127550 frames Cell Events: 4843 mean_circ: 0.933729
Ran for 128050 frames Cell Events: 4861 mean_circ: 0.933721
Ran for 128550 frames Cell Events: 4880 mean_circ: 0.933653
Ran for 129050 frames Cell Events: 4895 mean_circ: 0.933653
Ran for 129550 frames Cell Events: 4923 mean_circ: 0.933668
Ran for 130050 frames Cell Events: 4948 mean_circ: 0.933679
Ran for 130550 frames Cell Events: 4967 mean_circ: 0.933672
Ran for 131550 frames Cell Events: 5000 mean_circ: 0.933662
Ran for 132050 frames Cell Events: 5022 mean_circ: 0.93363
Ran for 132550 frames Cell Events: 5047 mean_circ: 0.933685
Ran for 133050 frames Cell Events: 5060 mean_circ: 0.93371
Ran for 133550 frames Cell Events: 5074 mean_circ: 0.933659
Ran for 135550 frames Cell Events: 5161 mean_circ: 0.933812
Ran for 136050 frames Cell Events: 5184 mean_circ: 0.933808
Ran for 137550 frames Cell Events: 5240 mean_circ: 0.933874
Ran for 138550 frames Cell Events: 5276 mean_circ: 0.933863
Ran for 139050 frames Cell Events: 5288 mean_circ: 0.933884
Ran for 140550 frames Cell Events: 5343 mean_circ: 0.933902
Ran for 141050 frames Cell Events: 5362 mean_circ: 0.933896
Ran for 141550 frames Cell Events: 5373 mean_circ: 0.933906
Ran for 142050 frames Cell Events: 5393 mean_circ: 0.933946
Ran for 143050 frames Cell Events: 5445 mean_circ: 0.933972
Ran for 143550 frames Cell Events: 5473 mean_circ: 0.933883
Ran for 146050 frames Cell Events: 5561 mean_circ: 0.9339
Ran for 146550 frames Cell Events: 5573 mean_circ: 0.933898
Ran for 147050 frames Cell Events: 5598 mean_circ: 0.933938
Ran for 148050 frames Cell Events: 5640 mean_circ: 0.933971
Ran for 148550 frames Cell Events: 5668 mean_circ: 0.934012
Ran for 149050 frames Cell Events: 5693 mean_circ: 0.934041
Ran for 151550 frames Cell Events: 5796 mean_circ: 0.933995
Ran for 152050 frames Cell Events: 5817 mean_circ: 0.933985
Ran for 152550 frames Cell Events: 5834 mean_circ: 0.933983
Ran for 153050 frames Cell Events: 5846 mean_circ: 0.934028
Ran for 153550 frames Cell Events: 5870 mean_circ: 0.933971
Ran for 154050 frames Cell Events: 5897 mean_circ: 0.934019
Ran for 155550 frames Cell Events: 5975 mean_circ: 0.933962
Ran for 156050 frames Cell Events: 6004 mean_circ: 0.93391
Ran for 156550 frames Cell Events: 6028 mean_circ: 0.93388
Ran for 157050 frames Cell Events: 6049 mean_circ: 0.933912
Ran for 158050 frames Cell Events: 6083 mean_circ: 0.933779
Ran for 158550 frames Cell Events: 6109 mean_circ: 0.9338
Ran for 159550 frames Cell Events: 6155 mean_circ: 0.933931
Ran for 160050 frames Cell Events: 6179 mean_circ: 0.933954
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Ran for 161550 frames Cell Events: 6235 mean_circ: 0.934031
Ran for 163050 frames Cell Events: 6282 mean_circ: 0.934062
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Ran for 166050 frames Cell Events: 6400 mean_circ: 0.934065
Ran for 166550 frames Cell Events: 6420 mean_circ: 0.9341
Ran for 167050 frames Cell Events: 6445 mean_circ: 0.934085
Ran for 167550 frames Cell Events: 6461 mean_circ: 0.93408
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Ran for 171550 frames Cell Events: 6628 mean_circ: 0.934031
Ran for 172050 frames Cell Events: 6637 mean_circ: 0.934023
Ran for 172550 frames Cell Events: 6658 mean_circ: 0.934041
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Ran for 183050 frames Cell Events: 7108 mean_circ: 0.933742
Ran for 183550 frames Cell Events: 7122 mean_circ: 0.933803
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Ran for 185550 frames Cell Events: 7210 mean_circ: 0.933821
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Ran for 189050 frames Cell Events: 7323 mean_circ: 0.933822
Ran for 190050 frames Cell Events: 7366 mean_circ: 0.933805
Ran for 190550 frames Cell Events: 7388 mean_circ: 0.933812
Ran for 191050 frames Cell Events: 7407 mean_circ: 0.933831
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Ran for 192550 frames Cell Events: 7475 mean_circ: 0.933832
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Ran for 193550 frames Cell Events: 7502 mean_circ: 0.933869
Ran for 195550 frames Cell Events: 7580 mean_circ: 0.933907
Ran for 196050 frames Cell Events: 7598 mean_circ: 0.933929
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Ran for 199550 frames Cell Events: 7726 mean_circ: 0.93387
Ran for 200050 frames Cell Events: 7739 mean_circ: 0.93383
Ran for 200550 frames Cell Events: 7749 mean_circ: 0.93384
Ran for 201050 frames Cell Events: 7771 mean_circ: 0.933811
Ran for 202050 frames Cell Events: 7820 mean_circ: 0.933897
Ran for 202550 frames Cell Events: 7853 mean_circ: 0.933863
Ran for 203050 frames Cell Events: 7880 mean_circ: 0.9339
Ran for 203550 frames Cell Events: 7905 mean_circ: 0.933848
Ran for 204550 frames Cell Events: 7945 mean_circ: 0.933922
Ran for 205550 frames Cell Events: 7976 mean_circ: 0.933914
Ran for 206550 frames Cell Events: 8020 mean_circ: 0.933929
Ran for 207050 frames Cell Events: 8042 mean_circ: 0.933927
End this measurment..
Stopping time: 5.65127e+008
****************************************
Measurement finished
Mean Circularitiy: 0.933891
Mean Area: 643.73
Mean FL-1 intensity: 3818
Mean FL-2 intensity: 3878.14
Mean FL-3 intensity: 0
frames processed: 206422
Total Time: 104254ms
Number of Runs (new bg img) 0
Frames collected: 208371
Skipped frames: 43959
Rescued Frames: 2485649
Samples DAQ: 52093225
Samples written: 104185678
Samples per Channel: 52092839
FPS: 1998.69
Samples per second: 499673
Expected samples/s: 500000
Events: 8096
Events per second: 77.6565
jumped back (PMT): 20
#Frames read, frequency
# 0 frames :296368
# 1 frames :103071
# 2 frames :5534
# 3 frames :1451
# 4 frames :998
# 5 frames :408
# 6 frames :1605
# 7 frames :2328
# 8 frames :1307
# 9 frames :830
# 10 frames :671
# 11 frames :472
# 12 frames :384
# 13 frames :238
# 14 frames :190
# 15 frames :203
# 16 frames :151
# 17 frames :116
# 18 frames :69
# 19 frames :66
# 20 frames :37
# 21 frames :37
# 22 frames :33
# 23 frames :24
# 24 frames :20
# 25 frames :15
# 26 frames :10
# 27 frames :9
# 28 frames :8
# 29 frames :8
# 30 frames :6
# 31 frames :2
# 32 frames :5
# 33 frames :2
# 34 frames :6
# 35 frames :2
# 36 frames :1
# 37 frames :1
# 39 frames :1
# 40 frames :1
# 41 frames :1
# 42 frames :1
# 43 frames :1
# 44 frames :2
# 45 frames :2
# 46 frames :1
# 52 frames :1
# 54 frames :1
# 60 frames :1
# 92 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9143
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0