Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_19.47.03
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "09d72fd84f5e3220e6dd9bd3a5d5739c",
"name": "M1_20170620_1711_NK_B2_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:47:23"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8204 (max 8205) in '8204'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.3657
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 5.68814e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 16 mean_circ: 0.965558
Ran for 1050 frames Cell Events: 31 mean_circ: 0.948126
Ran for 2050 frames Cell Events: 65 mean_circ: 0.943997
Ran for 3550 frames Cell Events: 116 mean_circ: 0.952604
Ran for 4050 frames Cell Events: 129 mean_circ: 0.952146
Ran for 4550 frames Cell Events: 145 mean_circ: 0.953223
Ran for 5050 frames Cell Events: 178 mean_circ: 0.954246
Ran for 6050 frames Cell Events: 208 mean_circ: 0.954128
Ran for 7550 frames Cell Events: 268 mean_circ: 0.954367
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Ran for 76550 frames Cell Events: 2664 mean_circ: 0.954259
Ran for 77050 frames Cell Events: 2683 mean_circ: 0.954306
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Ran for 82550 frames Cell Events: 2836 mean_circ: 0.954128
Ran for 83050 frames Cell Events: 2857 mean_circ: 0.954051
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Ran for 84550 frames Cell Events: 2920 mean_circ: 0.953927
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Ran for 87550 frames Cell Events: 3037 mean_circ: 0.953592
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Ran for 90050 frames Cell Events: 3123 mean_circ: 0.953674
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Ran for 92050 frames Cell Events: 3203 mean_circ: 0.953626
Ran for 93050 frames Cell Events: 3244 mean_circ: 0.953616
Ran for 94050 frames Cell Events: 3286 mean_circ: 0.953592
Ran for 95050 frames Cell Events: 3323 mean_circ: 0.953645
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Ran for 96550 frames Cell Events: 3372 mean_circ: 0.953603
Ran for 97050 frames Cell Events: 3392 mean_circ: 0.953632
Ran for 100050 frames Cell Events: 3504 mean_circ: 0.953442
Ran for 101050 frames Cell Events: 3540 mean_circ: 0.953405
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Ran for 102050 frames Cell Events: 3589 mean_circ: 0.953259
Ran for 103050 frames Cell Events: 3628 mean_circ: 0.953334
Ran for 103550 frames Cell Events: 3646 mean_circ: 0.95326
Ran for 104050 frames Cell Events: 3674 mean_circ: 0.95319
Ran for 104550 frames Cell Events: 3688 mean_circ: 0.953229
Ran for 105550 frames Cell Events: 3724 mean_circ: 0.953168
Ran for 106050 frames Cell Events: 3734 mean_circ: 0.953172
Ran for 106550 frames Cell Events: 3747 mean_circ: 0.953209
Ran for 107050 frames Cell Events: 3755 mean_circ: 0.953165
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Ran for 121550 frames Cell Events: 4285 mean_circ: 0.952701
Ran for 122050 frames Cell Events: 4295 mean_circ: 0.952727
Ran for 123050 frames Cell Events: 4326 mean_circ: 0.95275
Ran for 123550 frames Cell Events: 4342 mean_circ: 0.952795
Ran for 124050 frames Cell Events: 4355 mean_circ: 0.952798
Ran for 125050 frames Cell Events: 4379 mean_circ: 0.952858
Ran for 125550 frames Cell Events: 4400 mean_circ: 0.952859
Ran for 126050 frames Cell Events: 4415 mean_circ: 0.952844
Ran for 127550 frames Cell Events: 4473 mean_circ: 0.952769
Ran for 128050 frames Cell Events: 4495 mean_circ: 0.952748
Ran for 128550 frames Cell Events: 4511 mean_circ: 0.952743
Ran for 129050 frames Cell Events: 4524 mean_circ: 0.952744
Ran for 130050 frames Cell Events: 4563 mean_circ: 0.95278
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Ran for 131550 frames Cell Events: 4614 mean_circ: 0.952742
Ran for 132050 frames Cell Events: 4624 mean_circ: 0.952749
Ran for 132550 frames Cell Events: 4641 mean_circ: 0.952674
Ran for 133050 frames Cell Events: 4663 mean_circ: 0.95273
Ran for 133550 frames Cell Events: 4686 mean_circ: 0.952727
Ran for 134050 frames Cell Events: 4708 mean_circ: 0.952728
Ran for 136050 frames Cell Events: 4778 mean_circ: 0.952712
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Ran for 141050 frames Cell Events: 4979 mean_circ: 0.952671
Ran for 141550 frames Cell Events: 5000 mean_circ: 0.952637
Ran for 142050 frames Cell Events: 5026 mean_circ: 0.952631
Ran for 142550 frames Cell Events: 5046 mean_circ: 0.952632
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Ran for 193550 frames Cell Events: 6756 mean_circ: 0.951891
Ran for 194050 frames Cell Events: 6776 mean_circ: 0.951872
Ran for 194550 frames Cell Events: 6790 mean_circ: 0.951879
Ran for 195050 frames Cell Events: 6805 mean_circ: 0.951881
Ran for 195550 frames Cell Events: 6833 mean_circ: 0.951884
Ran for 196050 frames Cell Events: 6843 mean_circ: 0.951824
Ran for 196550 frames Cell Events: 6861 mean_circ: 0.951816
Ran for 197050 frames Cell Events: 6877 mean_circ: 0.951856
Ran for 198550 frames Cell Events: 6931 mean_circ: 0.951773
Ran for 199550 frames Cell Events: 6959 mean_circ: 0.951759
Ran for 201050 frames Cell Events: 7020 mean_circ: 0.9518
Ran for 201550 frames Cell Events: 7050 mean_circ: 0.951786
Ran for 202050 frames Cell Events: 7083 mean_circ: 0.951762
Ran for 202550 frames Cell Events: 7102 mean_circ: 0.951759
Ran for 203050 frames Cell Events: 7128 mean_circ: 0.951736
Ran for 203550 frames Cell Events: 7146 mean_circ: 0.95174
Ran for 204050 frames Cell Events: 7160 mean_circ: 0.951776
Ran for 204550 frames Cell Events: 7184 mean_circ: 0.951777
Ran for 205050 frames Cell Events: 7201 mean_circ: 0.951812
Ran for 205550 frames Cell Events: 7220 mean_circ: 0.951813
Ran for 206050 frames Cell Events: 7229 mean_circ: 0.951814
Ran for 207050 frames Cell Events: 7260 mean_circ: 0.951748
Ran for 207550 frames Cell Events: 7280 mean_circ: 0.951766
Ran for 208050 frames Cell Events: 7297 mean_circ: 0.951764
Ran for 208550 frames Cell Events: 7317 mean_circ: 0.95179
Ran for 209050 frames Cell Events: 7326 mean_circ: 0.951796
Ran for 209550 frames Cell Events: 7346 mean_circ: 0.951778
Ran for 210050 frames Cell Events: 7363 mean_circ: 0.951804
Ran for 210550 frames Cell Events: 7376 mean_circ: 0.951802
Ran for 211050 frames Cell Events: 7396 mean_circ: 0.951769
Ran for 211550 frames Cell Events: 7423 mean_circ: 0.95176
Ran for 212050 frames Cell Events: 7439 mean_circ: 0.951766
Ran for 212550 frames Cell Events: 7463 mean_circ: 0.951777
Ran for 213050 frames Cell Events: 7482 mean_circ: 0.951795
Ran for 213550 frames Cell Events: 7502 mean_circ: 0.951787
Ran for 214550 frames Cell Events: 7542 mean_circ: 0.951805
Ran for 215050 frames Cell Events: 7567 mean_circ: 0.951798
Ran for 215550 frames Cell Events: 7591 mean_circ: 0.951789
Ran for 216050 frames Cell Events: 7607 mean_circ: 0.951797
Ran for 216550 frames Cell Events: 7630 mean_circ: 0.951808
Ran for 217550 frames Cell Events: 7666 mean_circ: 0.951815
Ran for 218050 frames Cell Events: 7686 mean_circ: 0.951812
Ran for 218550 frames Cell Events: 7712 mean_circ: 0.951823
Ran for 220050 frames Cell Events: 7779 mean_circ: 0.951798
Ran for 220550 frames Cell Events: 7806 mean_circ: 0.951797
Ran for 221050 frames Cell Events: 7824 mean_circ: 0.951804
Ran for 222050 frames Cell Events: 7873 mean_circ: 0.951815
Ran for 222550 frames Cell Events: 7892 mean_circ: 0.951835
Ran for 224050 frames Cell Events: 7942 mean_circ: 0.951804
Ran for 225050 frames Cell Events: 7985 mean_circ: 0.951809
Ran for 225550 frames Cell Events: 8014 mean_circ: 0.951797
Ran for 226050 frames Cell Events: 8040 mean_circ: 0.951831
Ran for 226550 frames Cell Events: 8056 mean_circ: 0.951829
Ran for 227050 frames Cell Events: 8074 mean_circ: 0.951829
Ran for 228050 frames Cell Events: 8102 mean_circ: 0.951831
Ran for 228550 frames Cell Events: 8115 mean_circ: 0.95181
Ran for 230050 frames Cell Events: 8167 mean_circ: 0.951779
End this measurment..
Stopping time: 5.6893e+008
****************************************
Measurement finished
Mean Circularitiy: 0.951693
Mean Area: 630.133
Mean FL-1 intensity: 3769.46
Mean FL-2 intensity: 4658.12
Mean FL-3 intensity: 0
frames processed: 230274
Total Time: 115469ms
Number of Runs (new bg img) 0
Frames collected: 230761
Skipped frames: 57378
Rescued Frames: 3348787
Samples DAQ: 57690260
Samples written: 115380282
Samples per Channel: 57690141
FPS: 1998.47
Samples per second: 499617
Expected samples/s: 500000
Events: 8206
Events per second: 71.0668
jumped back (PMT): 5885
#Frames read, frequency
# 0 frames :324479
# 1 frames :119173
# 2 frames :5447
# 3 frames :1819
# 4 frames :1002
# 5 frames :417
# 6 frames :1160
# 7 frames :2475
# 8 frames :1002
# 9 frames :611
# 10 frames :1239
# 11 frames :687
# 12 frames :469
# 13 frames :314
# 14 frames :222
# 15 frames :207
# 16 frames :179
# 17 frames :146
# 18 frames :101
# 19 frames :58
# 20 frames :40
# 21 frames :48
# 22 frames :47
# 23 frames :18
# 24 frames :29
# 25 frames :14
# 26 frames :17
# 27 frames :7
# 28 frames :10
# 29 frames :7
# 30 frames :9
# 31 frames :11
# 32 frames :5
# 33 frames :1
# 34 frames :1
# 35 frames :2
# 36 frames :1
# 37 frames :3
# 38 frames :1
# 39 frames :1
# 40 frames :1
# 41 frames :1
# 42 frames :1
# 50 frames :1
# 51 frames :2
# 57 frames :1
# 60 frames :1
# 61 frames :1
# 62 frames :1
# 71 frames :1
# 74 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.3657
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0