M3_data_scalar.rtdc
| Date of measurement | 2019-01-25 |
|---|---|
| Index of measurement run | 3 |
| Sample name | CONTROL |
| Number of recorded events | 6808 |
| Start time of measurement | 15:25:19 |
| Unique measurement identifier | e3308df4-a370-ef01-f38a-b0eaf0104ec3-3888 |
| Software | ShapeIn 2.0.6 | dclab 0.60.3 | dclab 0.62.14 |
|---|---|
| Modules used | AcCellerator, FluorescenceModule |
| Flow rate in channel | 0.04 µL/s |
| Imaged chip region | channel |
| Medium used | 0.49% MC-PBS |
| Sample flow rate | 0.01 µL/s |
| Sheath flow rate | 0.03 µL/s |
| Unique setup identifier | ZMDD-AcC-fe3cdd-2a2250 |
| Width of microfluidic channel | 20 µm |
| Image height | 80 px |
|---|---|
| Image width | 250 px |
| Image x coordinate on sensor | 629 px |
| Image y coordinate on sensor | 512 px |
| Imaging frame rate | 1250 Hz |
| Light source device type | LED (ZMD L1) |
| Light source flash duration | 2 µs |
| Pixel size | 0.34 µm |
| FL1 description | 525/50 |
|---|---|
| FL2 description | 593/46 |
| Laser 1 output power | 32 % |
| Laser 1 wavelength | 488 nm |
| Laser 2 output power | 100 % |
| Laser 2 wavelength | 561 nm |
| Lower voltage detection limit | -1 V |
| Number of active channels | 2 |
| Number of active lasers | 2 |
| Number of available channels | 3 |
| Number of available lasers | 3 |
| Rolling median filter size for traces | 5 |
| Samples per event | 425 |
| Trace bit depth | 16 |
| Trace sample rate | 5e+05 Hz |
| Upper voltage detection limit | 1 V |
Additional Information
| Field | Value |
|---|---|
| license | Creative Commons Public Domain Dedication |
| created | 3 months ago |
| etag | d364963c750efe64d449187aaec5638f |
| format | RT-FDC |
| id | a2254022-e414-431a-b2e0-64bf056f3b1c |
| metadata modified | 3 months ago |
| mimetype | RT-DC |
| package id | f5565300-d5a5-4645-bd0f-70494727af6e |
| position | 4 |
| s3 available | True |
| s3 url | https://objectstore.hpccloud.mpcdf.mpg.de/circle-8a23b5b7-6d86-46b7-9833-62373e9ec110/resource/a22/540/22-e414-431a-b2e0-64bf056f3b1c |
| sha256 | 1a1cd97bd876e8ff704ef8ac81c6c77c94477e78e140808503355606aff30f74 |
| size | 287.5 KiB |
| dc:experiment:date | 2019-01-25 |
| dc:experiment:event count | 6,808 |
| dc:experiment:run identifier | e3308df4-a370-ef01-f38a-b0eaf0104ec3-3888 |
| dc:experiment:run index | 3 |
| dc:experiment:sample | CONTROL |
| dc:experiment:time | 15:25:19 |
| dc:fluorescence:bit depth | 16 |
| dc:fluorescence:channel 1 name | 525/50 |
| dc:fluorescence:channel 2 name | 593/46 |
| dc:fluorescence:channel count | 2 |
| dc:fluorescence:channels installed | 3 |
| dc:fluorescence:laser 1 lambda | 488 |
| dc:fluorescence:laser 1 power | 32 |
| dc:fluorescence:laser 2 lambda | 561 |
| dc:fluorescence:laser 2 power | 100 |
| dc:fluorescence:laser count | 2 |
| dc:fluorescence:lasers installed | 3 |
| dc:fluorescence:sample rate | 500,000 |
| dc:fluorescence:samples per event | 425 |
| dc:fluorescence:signal max | 1 |
| dc:fluorescence:signal min | -1 |
| dc:fluorescence:trace median | 5 |
| dc:imaging:flash device | LED (ZMD L1) |
| dc:imaging:flash duration | 2 |
| dc:imaging:frame rate | 1,250 |
| dc:imaging:pixel size | 0.34 |
| dc:imaging:roi position x | 629 |
| dc:imaging:roi position y | 512 |
| dc:imaging:roi size x | 250 |
| dc:imaging:roi size y | 80 |
| dc:online contour:bin area min | 10 |
| dc:online contour:bin kernel | 5 |
| dc:online contour:bin threshold | -6 |
| dc:online contour:image blur | 0 |
| dc:online contour:no absdiff | True |
| dc:online filter:target event count | 10,000 |
| dc:setup:channel width | 20 |
| dc:setup:chip region | channel |
| dc:setup:flow rate | 0.04 |
| dc:setup:flow rate sample | 0.01 |
| dc:setup:flow rate sheath | 0.03 |
| dc:setup:identifier | ZMDD-AcC-fe3cdd-2a2250 |
| dc:setup:medium | CellCarrier |
| dc:setup:module composition | AcCellerator,FluorescenceModule |
| dc:setup:software version | ShapeIn 2.0.6 | dclab 0.60.3 | dclab 0.62.14 |
| sp:cells:cell type | KC167 |
| sp:cells:live | True |
| sp:cells:organism | Drosophila melanogaster |
| sp:chip:channel width | 20 |
| sp:experiment:buffer osmolality | 325 |
| sp:experiment:buffer ph | 6.8 |
| sp:experiment:concentration | 5 |
| sp:identifiers:labels | GFP tdTomato |
| sp:identifiers:tags | NLS-GFP |