Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_18.18.38
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "ba8ade2c68e872eb2ee702ec9b2a0f44",
"name": "M1_20170519_1303_NK1_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "16:19:04"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8042 (max 8043) in '8042'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 27.1247
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.62425e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 16 mean_circ: 0.942812
Ran for 1550 frames Cell Events: 49 mean_circ: 0.947164
Ran for 3050 frames Cell Events: 87 mean_circ: 0.94953
Ran for 3550 frames Cell Events: 100 mean_circ: 0.950294
Ran for 4050 frames Cell Events: 113 mean_circ: 0.949721
Ran for 5050 frames Cell Events: 147 mean_circ: 0.950226
Ran for 5550 frames Cell Events: 158 mean_circ: 0.950276
Ran for 6050 frames Cell Events: 171 mean_circ: 0.949397
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Ran for 8050 frames Cell Events: 224 mean_circ: 0.948443
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Ran for 66550 frames Cell Events: 1947 mean_circ: 0.945541
Ran for 67050 frames Cell Events: 1961 mean_circ: 0.945458
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Ran for 81050 frames Cell Events: 2396 mean_circ: 0.945286
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Ran for 86050 frames Cell Events: 2540 mean_circ: 0.945293
Ran for 87050 frames Cell Events: 2571 mean_circ: 0.94534
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Ran for 90550 frames Cell Events: 2676 mean_circ: 0.945502
Ran for 92050 frames Cell Events: 2729 mean_circ: 0.945544
Ran for 92550 frames Cell Events: 2742 mean_circ: 0.945544
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Ran for 95550 frames Cell Events: 2852 mean_circ: 0.945558
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Ran for 97550 frames Cell Events: 2921 mean_circ: 0.945544
Ran for 98050 frames Cell Events: 2939 mean_circ: 0.945542
Ran for 100050 frames Cell Events: 3001 mean_circ: 0.94554
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Ran for 102550 frames Cell Events: 3062 mean_circ: 0.945438
Ran for 103550 frames Cell Events: 3098 mean_circ: 0.945358
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Ran for 106550 frames Cell Events: 3201 mean_circ: 0.945198
Ran for 107050 frames Cell Events: 3210 mean_circ: 0.945176
Ran for 107550 frames Cell Events: 3229 mean_circ: 0.945113
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Ran for 117550 frames Cell Events: 3533 mean_circ: 0.945011
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Ran for 121550 frames Cell Events: 3656 mean_circ: 0.944738
Ran for 122050 frames Cell Events: 3671 mean_circ: 0.944709
Ran for 122550 frames Cell Events: 3689 mean_circ: 0.944729
Ran for 123050 frames Cell Events: 3706 mean_circ: 0.944706
Ran for 124050 frames Cell Events: 3738 mean_circ: 0.944712
Ran for 125550 frames Cell Events: 3795 mean_circ: 0.944653
Ran for 126050 frames Cell Events: 3812 mean_circ: 0.944602
Ran for 127050 frames Cell Events: 3848 mean_circ: 0.944646
Ran for 127550 frames Cell Events: 3857 mean_circ: 0.944696
Ran for 128050 frames Cell Events: 3874 mean_circ: 0.944767
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Ran for 131050 frames Cell Events: 3968 mean_circ: 0.944559
Ran for 131550 frames Cell Events: 3985 mean_circ: 0.944551
Ran for 132550 frames Cell Events: 4017 mean_circ: 0.944548
Ran for 133050 frames Cell Events: 4030 mean_circ: 0.94457
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Ran for 172550 frames Cell Events: 5294 mean_circ: 0.944611
Ran for 173050 frames Cell Events: 5308 mean_circ: 0.944637
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Ran for 182050 frames Cell Events: 5580 mean_circ: 0.944616
Ran for 183550 frames Cell Events: 5625 mean_circ: 0.944685
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Ran for 186550 frames Cell Events: 5692 mean_circ: 0.944653
Ran for 187050 frames Cell Events: 5720 mean_circ: 0.94466
Ran for 188550 frames Cell Events: 5777 mean_circ: 0.944658
Ran for 189550 frames Cell Events: 5812 mean_circ: 0.944619
Ran for 190550 frames Cell Events: 5847 mean_circ: 0.944598
Ran for 191050 frames Cell Events: 5864 mean_circ: 0.94461
Ran for 191550 frames Cell Events: 5883 mean_circ: 0.944613
Ran for 192050 frames Cell Events: 5899 mean_circ: 0.94462
Ran for 192550 frames Cell Events: 5914 mean_circ: 0.944646
Ran for 193050 frames Cell Events: 5936 mean_circ: 0.944634
Ran for 194050 frames Cell Events: 5972 mean_circ: 0.944596
Ran for 194550 frames Cell Events: 5995 mean_circ: 0.944591
Ran for 195050 frames Cell Events: 6008 mean_circ: 0.944615
Ran for 195550 frames Cell Events: 6018 mean_circ: 0.944594
Ran for 196050 frames Cell Events: 6034 mean_circ: 0.944626
Ran for 197050 frames Cell Events: 6073 mean_circ: 0.944645
Ran for 198550 frames Cell Events: 6128 mean_circ: 0.944672
Ran for 199050 frames Cell Events: 6149 mean_circ: 0.944667
Ran for 199550 frames Cell Events: 6171 mean_circ: 0.94466
Ran for 200050 frames Cell Events: 6198 mean_circ: 0.94466
Ran for 200550 frames Cell Events: 6220 mean_circ: 0.944626
Ran for 201050 frames Cell Events: 6236 mean_circ: 0.94456
Ran for 201550 frames Cell Events: 6255 mean_circ: 0.944574
Ran for 202050 frames Cell Events: 6271 mean_circ: 0.944548
Ran for 202550 frames Cell Events: 6291 mean_circ: 0.944559
Ran for 204050 frames Cell Events: 6350 mean_circ: 0.944572
Ran for 204550 frames Cell Events: 6362 mean_circ: 0.944586
Ran for 205050 frames Cell Events: 6383 mean_circ: 0.944609
Ran for 205550 frames Cell Events: 6397 mean_circ: 0.944643
Ran for 207050 frames Cell Events: 6454 mean_circ: 0.944611
Ran for 207550 frames Cell Events: 6474 mean_circ: 0.944631
Ran for 208050 frames Cell Events: 6485 mean_circ: 0.944632
Ran for 208550 frames Cell Events: 6500 mean_circ: 0.944646
Ran for 209050 frames Cell Events: 6521 mean_circ: 0.944652
Ran for 210050 frames Cell Events: 6554 mean_circ: 0.94466
Ran for 211050 frames Cell Events: 6583 mean_circ: 0.944708
Ran for 211550 frames Cell Events: 6600 mean_circ: 0.944664
Ran for 212550 frames Cell Events: 6635 mean_circ: 0.944678
Ran for 214050 frames Cell Events: 6693 mean_circ: 0.944737
Ran for 214550 frames Cell Events: 6706 mean_circ: 0.944758
Ran for 215050 frames Cell Events: 6718 mean_circ: 0.944756
Ran for 215550 frames Cell Events: 6728 mean_circ: 0.94477
Ran for 216050 frames Cell Events: 6754 mean_circ: 0.944792
Ran for 217050 frames Cell Events: 6778 mean_circ: 0.94484
Ran for 218050 frames Cell Events: 6820 mean_circ: 0.944883
Ran for 218550 frames Cell Events: 6838 mean_circ: 0.944884
Ran for 219050 frames Cell Events: 6852 mean_circ: 0.944886
Ran for 220050 frames Cell Events: 6897 mean_circ: 0.944912
Ran for 220550 frames Cell Events: 6915 mean_circ: 0.944894
Ran for 221050 frames Cell Events: 6926 mean_circ: 0.94492
Ran for 221550 frames Cell Events: 6941 mean_circ: 0.944917
Ran for 222050 frames Cell Events: 6964 mean_circ: 0.944943
Ran for 224050 frames Cell Events: 7041 mean_circ: 0.944943
Ran for 224550 frames Cell Events: 7049 mean_circ: 0.944957
Ran for 225050 frames Cell Events: 7069 mean_circ: 0.944969
Ran for 225550 frames Cell Events: 7089 mean_circ: 0.944927
Ran for 226550 frames Cell Events: 7130 mean_circ: 0.945002
Ran for 227050 frames Cell Events: 7144 mean_circ: 0.945008
Ran for 227550 frames Cell Events: 7166 mean_circ: 0.944998
Ran for 228050 frames Cell Events: 7176 mean_circ: 0.945003
Ran for 228550 frames Cell Events: 7191 mean_circ: 0.945001
Ran for 229050 frames Cell Events: 7206 mean_circ: 0.944954
Ran for 229550 frames Cell Events: 7220 mean_circ: 0.944926
Ran for 230550 frames Cell Events: 7244 mean_circ: 0.944953
Ran for 231050 frames Cell Events: 7260 mean_circ: 0.944944
Ran for 231550 frames Cell Events: 7279 mean_circ: 0.944974
Ran for 232050 frames Cell Events: 7295 mean_circ: 0.945009
Ran for 232550 frames Cell Events: 7308 mean_circ: 0.945028
Ran for 233050 frames Cell Events: 7327 mean_circ: 0.945039
Ran for 234050 frames Cell Events: 7370 mean_circ: 0.945034
Ran for 234550 frames Cell Events: 7395 mean_circ: 0.945024
Ran for 235050 frames Cell Events: 7415 mean_circ: 0.944999
Ran for 235550 frames Cell Events: 7431 mean_circ: 0.944964
Ran for 236050 frames Cell Events: 7446 mean_circ: 0.944969
Ran for 236550 frames Cell Events: 7460 mean_circ: 0.944953
Ran for 237050 frames Cell Events: 7475 mean_circ: 0.944911
Ran for 237550 frames Cell Events: 7487 mean_circ: 0.944934
Ran for 238050 frames Cell Events: 7501 mean_circ: 0.944928
Ran for 239050 frames Cell Events: 7543 mean_circ: 0.944947
Ran for 239550 frames Cell Events: 7566 mean_circ: 0.944963
Ran for 240050 frames Cell Events: 7589 mean_circ: 0.944968
Ran for 240550 frames Cell Events: 7614 mean_circ: 0.944937
Ran for 241050 frames Cell Events: 7635 mean_circ: 0.944931
Ran for 241550 frames Cell Events: 7654 mean_circ: 0.944977
Ran for 242550 frames Cell Events: 7684 mean_circ: 0.944979
Ran for 243550 frames Cell Events: 7716 mean_circ: 0.945009
Ran for 244050 frames Cell Events: 7731 mean_circ: 0.945015
Ran for 244550 frames Cell Events: 7748 mean_circ: 0.945035
Ran for 245050 frames Cell Events: 7763 mean_circ: 0.945027
Ran for 245550 frames Cell Events: 7769 mean_circ: 0.945023
Ran for 246550 frames Cell Events: 7803 mean_circ: 0.945043
Ran for 247550 frames Cell Events: 7838 mean_circ: 0.94504
Ran for 248550 frames Cell Events: 7886 mean_circ: 0.945078
Ran for 249050 frames Cell Events: 7914 mean_circ: 0.945074
Ran for 249550 frames Cell Events: 7931 mean_circ: 0.945049
Ran for 250050 frames Cell Events: 7950 mean_circ: 0.945026
Ran for 250550 frames Cell Events: 7968 mean_circ: 0.945025
Ran for 251050 frames Cell Events: 7991 mean_circ: 0.945001
Ran for 251550 frames Cell Events: 8003 mean_circ: 0.945014
Ran for 252050 frames Cell Events: 8020 mean_circ: 0.945039
Ran for 252550 frames Cell Events: 8033 mean_circ: 0.94505
End this measurment..
Stopping time: 3.62552e+008
****************************************
Measurement finished
Mean Circularitiy: 0.94503
Mean Area: 614.917
Mean FL-1 intensity: 3283
Mean FL-2 intensity: 4618.31
Mean FL-3 intensity: 0
frames processed: 250702
Total Time: 126446ms
Number of Runs (new bg img) 0
Frames collected: 252732
Skipped frames: 2030
Rescued Frames: 89728
Samples DAQ: 63190033
Samples written: 126365956
Samples per Channel: 63182978
FPS: 1998.74
Samples per second: 499684
Expected samples/s: 500000
Events: 8043
Events per second: 63.6083
jumped back (PMT): 5239
#Frames read, frequency
# 0 frames :344293
# 1 frames :143796
# 2 frames :5232
# 3 frames :1582
# 4 frames :848
# 5 frames :313
# 6 frames :771
# 7 frames :3270
# 8 frames :1025
# 9 frames :1027
# 10 frames :1028
# 11 frames :393
# 12 frames :292
# 13 frames :361
# 14 frames :150
# 15 frames :141
# 16 frames :166
# 17 frames :136
# 18 frames :84
# 19 frames :103
# 20 frames :40
# 21 frames :34
# 22 frames :40
# 23 frames :23
# 24 frames :17
# 25 frames :18
# 26 frames :15
# 27 frames :12
# 28 frames :10
# 29 frames :10
# 30 frames :8
# 31 frames :6
# 32 frames :6
# 33 frames :4
# 35 frames :1
# 37 frames :3
# 38 frames :3
# 39 frames :1
# 40 frames :1
# 41 frames :1
# 43 frames :2
# 47 frames :1
# 58 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 27.1247
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0