figshare_dclab_7771184.v2_calibration_beads.rtdc
| Date of measurement | 2018-12-11 |
|---|---|
| Index of measurement run | 1 |
| Sample name | calibration_beads |
| Number of recorded events | 5000 |
| Start time of measurement | 10:04:03 |
| Software | ShapeIn 2.0.5 | dclab 0.58.6 |
|---|---|
| Modules used | AcCellerator, FluorescenceModule |
| Flow rate in channel | 0.06 µL/s |
| Imaged chip region | channel |
| Medium used | 0.59% MC-PBS |
| Sample flow rate | 0.015 µL/s |
| Sheath flow rate | 0.045 µL/s |
| Unique setup identifier | ZMDD-AcC-fe3cdd-2a2250 |
| Width of microfluidic channel | 20 µm |
| Image height | 80 px |
|---|---|
| Image width | 250 px |
| Image x coordinate on sensor | 629 px |
| Image y coordinate on sensor | 512 px |
| Imaging frame rate | 1600 Hz |
| Light source device type | LED (ZMD L1) |
| Light source flash duration | 2 µs |
| Pixel size | 0.34 µm |
| FL1 description | 525/50 |
|---|---|
| FL2 description | 593/46 |
| FL3 description | 700/75 |
| Laser 1 output power | 8 % |
| Laser 1 wavelength | 488 nm |
| Laser 3 output power | 100 % |
| Laser 3 wavelength | 640 nm |
| Lower voltage detection limit | -1 V |
| Number of active channels | 3 |
| Number of active lasers | 2 |
| Number of available channels | 3 |
| Number of available lasers | 3 |
| Rolling median filter size for traces | 0 |
| Samples per event | 177 |
| Trace bit depth | 16 |
| Trace sample rate | 3.125e+05 Hz |
| Upper voltage detection limit | 1 V |
Additional Information
| Field | Value |
|---|---|
| license | Creative Commons Attribution Share-Alike 4.0 |
| created | 1 year ago |
| etag | ccc2f870ee935802eaacc3fb866d5caa |
| format | RT-FDC |
| id | 578d64b9-b445-4b8f-a0fb-dff1b9e08cb1 |
| metadata modified | 1 year ago |
| mimetype | RT-DC |
| package id | d62c9f4e-b701-4cad-9330-4e6af2b393bc |
| position | 1 |
| s3 available | True |
| s3 url | https://objectstore.hpccloud.mpcdf.mpg.de/circle-91a014f0-6976-4657-9d3b-ac0e329639bf/resource/578/d64/b9-b445-4b8f-a0fb-dff1b9e08cb1 |
| sha256 | 649a6e5c476c188d0c921a2d40626e96985aaebd8c5b152211958a6c47363f2a |
| size | 66.4 MiB |
| dc:experiment:date | 2018-12-11 |
| dc:experiment:event count | 5,000 |
| dc:experiment:run index | 1 |
| dc:experiment:sample | calibration_beads |
| dc:experiment:time | 10:04:03 |
| dc:fluorescence:bit depth | 16 |
| dc:fluorescence:channel 1 name | 525/50 |
| dc:fluorescence:channel 2 name | 593/46 |
| dc:fluorescence:channel 3 name | 700/75 |
| dc:fluorescence:channel count | 3 |
| dc:fluorescence:channels installed | 3 |
| dc:fluorescence:laser 1 lambda | 488 |
| dc:fluorescence:laser 1 power | 8 |
| dc:fluorescence:laser 3 lambda | 640 |
| dc:fluorescence:laser 3 power | 100 |
| dc:fluorescence:laser count | 2 |
| dc:fluorescence:lasers installed | 3 |
| dc:fluorescence:sample rate | 312,500 |
| dc:fluorescence:samples per event | 177 |
| dc:fluorescence:signal max | 1 |
| dc:fluorescence:signal min | -1 |
| dc:fluorescence:trace median | 0 |
| dc:imaging:flash device | LED (ZMD L1) |
| dc:imaging:flash duration | 2 |
| dc:imaging:frame rate | 1,600 |
| dc:imaging:pixel size | 0.34 |
| dc:imaging:roi position x | 629 |
| dc:imaging:roi position y | 512 |
| dc:imaging:roi size x | 250 |
| dc:imaging:roi size y | 80 |
| dc:online contour:bin area min | 10 |
| dc:online contour:bin kernel | 5 |
| dc:online contour:bin threshold | -6 |
| dc:online contour:image blur | 0 |
| dc:online contour:no absdiff | True |
| dc:online filter:target event count | 5,000 |
| dc:setup:channel width | 20 |
| dc:setup:chip region | channel |
| dc:setup:flow rate | 0.06 |
| dc:setup:flow rate sample | 0.015 |
| dc:setup:flow rate sheath | 0.045 |
| dc:setup:identifier | ZMDD-AcC-fe3cdd-2a2250 |
| dc:setup:medium | CellCarrierB |
| dc:setup:module composition | AcCellerator,FluorescenceModule |
| dc:setup:software version | ShapeIn 2.0.5 | dclab 0.58.6 |