Thymus_TG.rtdc
| Date of measurement | 2019-06-14 |
|---|---|
| Index of measurement run | 2 |
| Sample name | Thymus TG |
| Number of recorded events | 4.892e+04 |
| Start time of measurement | 18:39:08 |
| Software | ShapeIn 2.0.8 | dclab 0.38.2 | dclab 0.47.2 |
|---|---|
| Modules used | AcCellerator, FluorescenceModule |
| Flow rate in channel | 0.12 µL/s |
| Imaged chip region | channel |
| Medium used | 0.59% MC-PBS |
| Sample flow rate | 0.03 µL/s |
| Sheath flow rate | 0.09 µL/s |
| Unique setup identifier | ZMDD-AcC-47be2b-bd1d61 |
| Width of microfluidic channel | 30 µm |
| Image height | 100 px |
|---|---|
| Image width | 250 px |
| Image x coordinate on sensor | 605 px |
| Image y coordinate on sensor | 580 px |
| Imaging frame rate | 1600 Hz |
| Light source device type | LED (ZMD L1) |
| Light source flash duration | 2 µs |
| Pixel size | 0.339 µm |
| FL1 description | 525/50 Green |
|---|---|
| FL2 description | 593/46 Yellow |
| FL3 description | 700/75 Red |
| Laser 1 output power | 30 % |
| Laser 1 wavelength | 488 nm |
| Laser 2 output power | 30 % |
| Laser 2 wavelength | 561 nm |
| Laser 3 output power | 30 % |
| Laser 3 wavelength | 640 nm |
| Lower voltage detection limit | -1 V |
| Number of active channels | 3 |
| Number of active lasers | 3 |
| Number of available channels | 3 |
| Number of available lasers | 3 |
| Rolling median filter size for traces | 5 |
| Samples per event | 397 |
| Trace bit depth | 16 |
| Trace sample rate | 6.25e+05 Hz |
| Upper voltage detection limit | 1 V |
Additional Information
| Field | Value |
|---|---|
| license | Creative Commons Public Domain Dedication |
| created | 2 years ago |
| format | RT-FDC |
| id | 15dd8d56-83d9-4b74-b7b0-38cccb88342e |
| metadata modified | 1 year ago |
| mimetype | RT-DC |
| package id | 96ade6cb-3d46-40fd-88b6-d9c59a9ae330 |
| position | 2 |
| s3 available | True |
| s3 url | https://objectstore.hpccloud.mpcdf.mpg.de/circle-13748aef-a5b5-47b4-b86d-8a0c1ff49035/resource/15d/d8d/56-83d9-4b74-b7b0-38cccb88342e |
| sha256 | 0e116665ebdca311928ff67b25039ba1cf6f2a4aaab82914beead971fb25b4c4 |
| size | 766.4 MiB |
| dc:experiment:date | 2019-06-14 |
| dc:experiment:event count | 48,922 |
| dc:experiment:run index | 2 |
| dc:experiment:sample | Thymus TG |
| dc:experiment:time | 18:39:08 |
| dc:fluorescence:bit depth | 16 |
| dc:fluorescence:channel 1 name | 525/50 Green |
| dc:fluorescence:channel 2 name | 593/46 Yellow |
| dc:fluorescence:channel 3 name | 700/75 Red |
| dc:fluorescence:channel count | 3 |
| dc:fluorescence:channels installed | 3 |
| dc:fluorescence:laser 1 lambda | 488 |
| dc:fluorescence:laser 1 power | 30 |
| dc:fluorescence:laser 2 lambda | 561 |
| dc:fluorescence:laser 2 power | 30 |
| dc:fluorescence:laser 3 lambda | 640 |
| dc:fluorescence:laser 3 power | 30 |
| dc:fluorescence:laser count | 3 |
| dc:fluorescence:lasers installed | 3 |
| dc:fluorescence:sample rate | 625,000 |
| dc:fluorescence:samples per event | 397 |
| dc:fluorescence:signal max | 1 |
| dc:fluorescence:signal min | -1 |
| dc:fluorescence:trace median | 5 |
| dc:imaging:flash device | LED (ZMD L1) |
| dc:imaging:flash duration | 2 |
| dc:imaging:frame rate | 1,600 |
| dc:imaging:pixel size | 0.339 |
| dc:imaging:roi position x | 605 |
| dc:imaging:roi position y | 580 |
| dc:imaging:roi size x | 250 |
| dc:imaging:roi size y | 100 |
| dc:online contour:bin area min | 10 |
| dc:online contour:bin kernel | 5 |
| dc:online contour:bin threshold | -6 |
| dc:online contour:image blur | 0 |
| dc:online contour:no absdiff | True |
| dc:setup:channel width | 30 |
| dc:setup:chip region | channel |
| dc:setup:flow rate | 0.12 |
| dc:setup:flow rate sample | 0.03 |
| dc:setup:flow rate sheath | 0.09 |
| dc:setup:identifier | ZMDD-AcC-47be2b-bd1d61 |
| dc:setup:medium | CellCarrierB |
| dc:setup:module composition | AcCellerator,FluorescenceModule |
| dc:setup:software version | ShapeIn 2.0.8 | dclab 0.38.2 | dclab 0.47.2 |