CD34_HSPC.rtdc
| Date of measurement | 2017-02-09 |
|---|---|
| Index of measurement run | 1 |
| Sample name | HSC_apher_raw_APC |
| Number of recorded events | 1.12e+05 |
| Start time of measurement | 15:13:04 |
| Software | fRT-DC V1.06170206 | dclab 0.9.1 |
|---|---|
| Modules used | Cell_Flow_2, Fluor |
| Flow rate in channel | 0.06 µL/s |
| Imaged chip region | channel |
| Medium used | 0.49% MC-PBS |
| Sample flow rate | 0.015 µL/s |
| Sheath flow rate | 0.045 µL/s |
| Unique setup identifier | Custom_RT-FDC |
| Width of microfluidic channel | 20 µm |
| Image height | 96 px |
|---|---|
| Image width | 256 px |
| Image x coordinate on sensor | 504 px |
| Image y coordinate on sensor | 472 px |
| Imaging frame rate | 2000 Hz |
| Light source device type | LED (undefined) |
| Light source flash duration | 2 µs |
| Pixel size | 0.34 µm |
| FL3 description | 700/75 |
|---|---|
| Laser 3 output power | 100 % |
| Laser 3 wavelength | 640 nm |
| Lower voltage detection limit | -1 V |
| Number of active channels | 1 |
| Number of active lasers | 1 |
| Number of available channels | 3 |
| Number of available lasers | 3 |
| Rolling median filter size for traces | 20 |
| Samples per event | 400 |
| Trace bit depth | 16 |
| Trace sample rate | 1e+06 Hz |
| Upper voltage detection limit | 1 V |
Additional Information
| Field | Value |
|---|---|
| license | Creative Commons Public Domain Dedication |
| created | 4 years ago |
| format | RT-FDC |
| id | eb98752d-e92d-e34e-4174-d108a276aedc |
| metadata modified | 1 year ago |
| mimetype | RT-DC |
| package id | 89bf2177-ffeb-9893-83cc-b619fc2f6663 |
| position | 0 |
| s3 available | True |
| s3 url | https://objectstore.hpccloud.mpcdf.mpg.de/circle-5a7a053d-55fb-4f99-960c-f478d0bd418f/resource/eb9/875/2d-e92d-e34e-4174-d108a276aedc |
| sha256 | 663b44a9db88d85996500045489e37a317cf115719223a531d617f8e3d450e79 |
| size | 1.9 GiB |
| dc:experiment:date | 2017-02-09 |
| dc:experiment:event count | 112,000 |
| dc:experiment:run index | 1 |
| dc:experiment:sample | HSC_apher_raw_APC |
| dc:experiment:time | 15:13:04 |
| dc:fluorescence:bit depth | 16 |
| dc:fluorescence:channel 3 name | 700/75 |
| dc:fluorescence:channel count | 1 |
| dc:fluorescence:channels installed | 3 |
| dc:fluorescence:laser 3 lambda | 640 |
| dc:fluorescence:laser 3 power | 100 |
| dc:fluorescence:laser count | 1 |
| dc:fluorescence:lasers installed | 3 |
| dc:fluorescence:sample rate | 1,000,000 |
| dc:fluorescence:samples per event | 400 |
| dc:fluorescence:signal max | 1 |
| dc:fluorescence:signal min | -1 |
| dc:fluorescence:trace median | 20 |
| dc:imaging:flash device | LED (undefined) |
| dc:imaging:flash duration | 2 |
| dc:imaging:frame rate | 2,000 |
| dc:imaging:pixel size | 0.34 |
| dc:imaging:roi position x | 504 |
| dc:imaging:roi position y | 472 |
| dc:imaging:roi size x | 256 |
| dc:imaging:roi size y | 96 |
| dc:online contour:bin area min | 50 |
| dc:online contour:bin kernel | 5 |
| dc:online contour:bin threshold | 6 |
| dc:online contour:image blur | 0 |
| dc:online contour:no absdiff | True |
| dc:setup:channel width | 20 |
| dc:setup:chip region | channel |
| dc:setup:flow rate | 0.06 |
| dc:setup:flow rate sample | 0.015 |
| dc:setup:flow rate sheath | 0.045 |
| dc:setup:identifier | Custom_RT-FDC |
| dc:setup:medium | CellCarrier |
| dc:setup:module composition | Cell_Flow_2, Fluor |
| dc:setup:software version | fRT-DC V1.06170206 | dclab 0.9.1 |