Real-time deformability cytometry reference data

This dataset consists of four exemplary real-time fluorescence and deformability cytometry measurements. The HDF5-files can be opened with dclab [1] or Shape-Out [2].

calibration_beads.rtdc
The calibartion beads (8 Peaks, PolyAN) consist of eight bead populations with different mixtures of fluorophores.

CD34_HSPC.rtdc
Hematopoietic stem and progenitor cells (HSPCs) were obtained using apheresis. The cells were tagged with a fluorescently labeled antibody that binds to the CD34 transmembrane protein. CD34-positive HSPCs are gated with fl3_max > 90. Set area_ratio < 1.05 to remove aggregates. Data were used in [3].

leukocytes.rtdc
The leukocyte population (white blood cells) of this blood sample can be visualized by setting aspect < 2 and area_ratio < 1.05. For more information, see e.g. [4].

reticulocytes.rtdc
Blood contains mostly red blood cells (RBCs) and about 1% reticulocytes (which develop into mature RBCs). Reticulocytes contain ribosomal RNA which was stained with Syto13 for this measurement. Set area_ratio < 1.05 to remove aggregates. Data were used in [3].

[1] https://github.com/ZellMechanik-Dresden/dclab

[2] https://github.com/ZellMechanik-Dresden/ShapeOut

[3] Rosendahl et al., "Real-time fluorescence and deformability cytometry". Nature Methods, 15(5):355–358, 2018. doi:10.1038/nmeth.4639.

[4] Toepfner et al., "Detection of human disease conditions by single-cell morpho-rheological phenotyping of whole blood". eLife, 7:e29213, 2017. doi:10.1101/145078.

SHA256 sums:
08c2ef13eed903ef0f9e451727ab8484df09b5d3b39227dab726e0164dcbe244 calibration_beads.rtdc
663b44a9db88d85996500045489e37a317cf115719223a531d617f8e3d450e79 CD34_HSPC.rtdc
68bd538b42ffb990f1db52d5f3b21f37c9aff31208ab284f3910fd6872c40fdb leukocytes.rtdc
5c323ea75bf7eeb2a28d922730772d50270dd872d6957e60d6062663f3628fb3 reticulocytes.rtdc

Data and Resources

Additional Info

Field Value
Last Updated September 16, 2020, 18:06 (+0200)
Created September 16, 2020, 17:58 (+0200)
Authors Philipp Rosendahl, Christoph Herold, Paul Müller, Jochen Guck
DOI 10.6084/m9.figshare.7771184.v2
References doi:10.1038/nmeth.4639 doi:10.1101/145078 https://github.com/ZellMechanik-Dresden/ShapeOut https://github.com/ZellMechanik-Dresden/dclab
License Creative Commons Public Domain Dedication
Dataset ID 89bf2177-ffeb-9893-83cc-b619fc2f6663