Blind_Colon__frozen_Sample_2.rtdc
| Date of measurement | 2020-11-12 |
|---|---|
| Index of measurement run | 1 |
| Sample name | Blind_Colon_ frozen_Sample 2 |
| Number of recorded events | 1.543e+05 |
| Start time of measurement | 13:06:12 |
| Software | 2.1.1.0 | dclab 0.38.2 |
|---|---|
| Modules used | AcCellerator, FluorescenceModule |
| Flow rate in channel | 0.06 µL/s |
| Imaged chip region | channel |
| Mean chip temperature | 25.8 °C |
| Medium used | 0.59% MC-PBS |
| Sample flow rate | 0.015 µL/s |
| Sheath flow rate | 0.045 µL/s |
| Unique setup identifier | ZMDD-AcC-774f84-3da488 |
| Width of microfluidic channel | 20 µm |
| Image height | 80 px |
|---|---|
| Image width | 250 px |
| Image x coordinate on sensor | 557 px |
| Image y coordinate on sensor | 470 px |
| Imaging frame rate | 1600 Hz |
| Light source device type | LED (ZMD L1) |
| Light source flash duration | 2 µs |
| Pixel size | 0.339 µm |
| FL1 description | 525/50 |
|---|---|
| FL2 description | 593/46 |
| FL3 description | 700/75 |
| Laser 1 output power | 2 % |
| Laser 1 wavelength | 488 nm |
| Laser 2 output power | 0.2 % |
| Laser 2 wavelength | 561 nm |
| Laser 3 output power | 70 % |
| Laser 3 wavelength | 640 nm |
| Lower voltage detection limit | -1 V |
| Number of active channels | 3 |
| Number of active lasers | 3 |
| Number of available channels | 3 |
| Number of available lasers | 3 |
| Rolling median filter size for traces | 5 |
| Samples per event | 353 |
| Trace bit depth | 16 |
| Trace sample rate | 6.25e+05 Hz |
| Upper voltage detection limit | 1 V |
Additional Information
| Field | Value |
|---|---|
| license | Creative Commons Public Domain Dedication |
| created | 2 years ago |
| format | RT-FDC |
| id | d9dd4607-24b4-420e-b9b2-009b5a66a256 |
| metadata modified | 1 year ago |
| mimetype | RT-DC |
| package id | 342b1d2e-5e1d-47ed-88c2-7871243f739a |
| position | 1 |
| s3 available | True |
| s3 url | https://objectstore.hpccloud.mpcdf.mpg.de/circle-13748aef-a5b5-47b4-b86d-8a0c1ff49035/resource/d9d/d46/07-24b4-420e-b9b2-009b5a66a256 |
| sha256 | 21c7681715cfea044d23267fff1f16a46b68e32f7a8ef6ac513f8831c432f532 |
| size | 1.8 GiB |
| dc:experiment:date | 2020-11-12 |
| dc:experiment:event count | 154,336 |
| dc:experiment:run index | 1 |
| dc:experiment:sample | Blind_Colon_ frozen_Sample 2 |
| dc:experiment:time | 13:06:12 |
| dc:fluorescence:bit depth | 16 |
| dc:fluorescence:channel 1 name | 525/50 |
| dc:fluorescence:channel 2 name | 593/46 |
| dc:fluorescence:channel 3 name | 700/75 |
| dc:fluorescence:channel count | 3 |
| dc:fluorescence:channels installed | 3 |
| dc:fluorescence:laser 1 lambda | 488 |
| dc:fluorescence:laser 1 power | 2 |
| dc:fluorescence:laser 2 lambda | 561 |
| dc:fluorescence:laser 2 power | 0.2 |
| dc:fluorescence:laser 3 lambda | 640 |
| dc:fluorescence:laser 3 power | 70 |
| dc:fluorescence:laser count | 3 |
| dc:fluorescence:lasers installed | 3 |
| dc:fluorescence:sample rate | 625,000 |
| dc:fluorescence:samples per event | 353 |
| dc:fluorescence:signal max | 1 |
| dc:fluorescence:signal min | -1 |
| dc:fluorescence:trace median | 5 |
| dc:imaging:flash device | LED (ZMD L1) |
| dc:imaging:flash duration | 2 |
| dc:imaging:frame rate | 1,600 |
| dc:imaging:pixel size | 0.339 |
| dc:imaging:roi position x | 557 |
| dc:imaging:roi position y | 470 |
| dc:imaging:roi size x | 250 |
| dc:imaging:roi size y | 80 |
| dc:online contour:bin area min | 10 |
| dc:online contour:bin kernel | 5 |
| dc:online contour:bin threshold | -6 |
| dc:online contour:image blur | 0 |
| dc:online contour:no absdiff | True |
| dc:setup:channel width | 20 |
| dc:setup:chip region | channel |
| dc:setup:flow rate | 0.06 |
| dc:setup:flow rate sample | 0.015 |
| dc:setup:flow rate sheath | 0.045 |
| dc:setup:identifier | ZMDD-AcC-774f84-3da488 |
| dc:setup:medium | CellCarrierB |
| dc:setup:module composition | AcCellerator,FluorescenceModule |
| dc:setup:software version | 2.1.1.0 | dclab 0.38.2 |
| dc:setup:temperature | 25.8 |