Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_11.47.44
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "f7c41cd5e5bb5b5a0912dc1fa412c287",
"name": "M1_20170530_1912_dsRNA_A1_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "09:48:06"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8158 (max 8159) in '8158'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6261
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.33497e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 17 mean_circ: 0.907466
Ran for 1050 frames Cell Events: 27 mean_circ: 0.927443
Ran for 1550 frames Cell Events: 44 mean_circ: 0.934717
Ran for 2050 frames Cell Events: 57 mean_circ: 0.935845
Ran for 2550 frames Cell Events: 71 mean_circ: 0.931867
Ran for 3050 frames Cell Events: 89 mean_circ: 0.935665
Ran for 3550 frames Cell Events: 99 mean_circ: 0.935637
Ran for 4050 frames Cell Events: 118 mean_circ: 0.936148
Ran for 4550 frames Cell Events: 126 mean_circ: 0.937271
Ran for 5050 frames Cell Events: 142 mean_circ: 0.937864
Ran for 6550 frames Cell Events: 184 mean_circ: 0.935974
Ran for 7550 frames Cell Events: 216 mean_circ: 0.935749
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Ran for 14550 frames Cell Events: 393 mean_circ: 0.938424
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Ran for 63050 frames Cell Events: 1948 mean_circ: 0.94
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Ran for 64550 frames Cell Events: 1986 mean_circ: 0.939805
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Ran for 67050 frames Cell Events: 2077 mean_circ: 0.939834
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Ran for 82050 frames Cell Events: 2564 mean_circ: 0.939456
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Ran for 83550 frames Cell Events: 2614 mean_circ: 0.939603
Ran for 84050 frames Cell Events: 2637 mean_circ: 0.939706
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Ran for 87050 frames Cell Events: 2735 mean_circ: 0.939766
Ran for 88050 frames Cell Events: 2772 mean_circ: 0.9399
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Ran for 93050 frames Cell Events: 2922 mean_circ: 0.939759
Ran for 93550 frames Cell Events: 2938 mean_circ: 0.939829
Ran for 94550 frames Cell Events: 2974 mean_circ: 0.939794
Ran for 96050 frames Cell Events: 3050 mean_circ: 0.939755
Ran for 96550 frames Cell Events: 3066 mean_circ: 0.939826
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Ran for 100050 frames Cell Events: 3210 mean_circ: 0.939862
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Ran for 110550 frames Cell Events: 3642 mean_circ: 0.940214
Ran for 111050 frames Cell Events: 3667 mean_circ: 0.940172
Ran for 111550 frames Cell Events: 3686 mean_circ: 0.940196
Ran for 112050 frames Cell Events: 3705 mean_circ: 0.940226
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Ran for 116050 frames Cell Events: 3851 mean_circ: 0.940082
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Ran for 121550 frames Cell Events: 4033 mean_circ: 0.94035
Ran for 122050 frames Cell Events: 4049 mean_circ: 0.940307
Ran for 123050 frames Cell Events: 4082 mean_circ: 0.940233
Ran for 123550 frames Cell Events: 4098 mean_circ: 0.940166
Ran for 124050 frames Cell Events: 4117 mean_circ: 0.940183
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Ran for 125550 frames Cell Events: 4168 mean_circ: 0.940195
Ran for 126050 frames Cell Events: 4180 mean_circ: 0.940207
Ran for 126550 frames Cell Events: 4206 mean_circ: 0.940176
Ran for 127050 frames Cell Events: 4227 mean_circ: 0.940176
Ran for 127550 frames Cell Events: 4237 mean_circ: 0.940105
Ran for 128050 frames Cell Events: 4249 mean_circ: 0.940059
Ran for 129050 frames Cell Events: 4307 mean_circ: 0.940086
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Ran for 131050 frames Cell Events: 4387 mean_circ: 0.940246
Ran for 132050 frames Cell Events: 4411 mean_circ: 0.940274
Ran for 133050 frames Cell Events: 4436 mean_circ: 0.940213
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Ran for 162550 frames Cell Events: 5443 mean_circ: 0.940779
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Ran for 179050 frames Cell Events: 6022 mean_circ: 0.94075
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Ran for 182050 frames Cell Events: 6120 mean_circ: 0.940673
Ran for 183050 frames Cell Events: 6157 mean_circ: 0.940705
Ran for 183550 frames Cell Events: 6184 mean_circ: 0.940633
Ran for 184550 frames Cell Events: 6232 mean_circ: 0.940636
Ran for 185050 frames Cell Events: 6254 mean_circ: 0.940675
Ran for 185550 frames Cell Events: 6265 mean_circ: 0.940664
Ran for 186050 frames Cell Events: 6291 mean_circ: 0.940679
Ran for 187050 frames Cell Events: 6325 mean_circ: 0.940699
Ran for 188050 frames Cell Events: 6359 mean_circ: 0.940761
Ran for 189050 frames Cell Events: 6390 mean_circ: 0.940857
Ran for 189550 frames Cell Events: 6398 mean_circ: 0.940871
Ran for 190550 frames Cell Events: 6445 mean_circ: 0.940951
Ran for 191050 frames Cell Events: 6456 mean_circ: 0.940975
Ran for 191550 frames Cell Events: 6478 mean_circ: 0.940974
Ran for 192550 frames Cell Events: 6516 mean_circ: 0.94096
Ran for 193050 frames Cell Events: 6534 mean_circ: 0.940983
Ran for 193550 frames Cell Events: 6545 mean_circ: 0.940973
Ran for 194050 frames Cell Events: 6561 mean_circ: 0.941001
Ran for 194550 frames Cell Events: 6588 mean_circ: 0.941028
Ran for 195050 frames Cell Events: 6601 mean_circ: 0.941048
Ran for 195550 frames Cell Events: 6614 mean_circ: 0.941049
Ran for 196050 frames Cell Events: 6634 mean_circ: 0.941096
Ran for 197050 frames Cell Events: 6669 mean_circ: 0.941152
Ran for 197550 frames Cell Events: 6689 mean_circ: 0.941134
Ran for 198050 frames Cell Events: 6705 mean_circ: 0.941171
Ran for 199050 frames Cell Events: 6734 mean_circ: 0.941226
Ran for 200050 frames Cell Events: 6770 mean_circ: 0.941187
Ran for 200550 frames Cell Events: 6783 mean_circ: 0.94122
Ran for 202550 frames Cell Events: 6863 mean_circ: 0.941138
Ran for 203050 frames Cell Events: 6878 mean_circ: 0.941131
Ran for 203550 frames Cell Events: 6893 mean_circ: 0.94113
Ran for 205050 frames Cell Events: 6943 mean_circ: 0.941088
Ran for 205550 frames Cell Events: 6956 mean_circ: 0.94109
Ran for 206050 frames Cell Events: 6969 mean_circ: 0.941044
Ran for 206550 frames Cell Events: 6984 mean_circ: 0.941051
Ran for 207050 frames Cell Events: 6995 mean_circ: 0.941061
Ran for 208550 frames Cell Events: 7059 mean_circ: 0.941005
Ran for 209050 frames Cell Events: 7078 mean_circ: 0.940977
Ran for 209550 frames Cell Events: 7100 mean_circ: 0.940973
Ran for 210050 frames Cell Events: 7127 mean_circ: 0.940972
Ran for 211050 frames Cell Events: 7147 mean_circ: 0.940966
Ran for 212050 frames Cell Events: 7187 mean_circ: 0.940987
Ran for 213050 frames Cell Events: 7223 mean_circ: 0.940942
Ran for 213550 frames Cell Events: 7231 mean_circ: 0.940953
Ran for 214050 frames Cell Events: 7249 mean_circ: 0.940964
Ran for 215050 frames Cell Events: 7274 mean_circ: 0.940978
Ran for 215550 frames Cell Events: 7279 mean_circ: 0.940965
Ran for 216050 frames Cell Events: 7300 mean_circ: 0.940982
Ran for 216550 frames Cell Events: 7310 mean_circ: 0.941004
Ran for 217050 frames Cell Events: 7324 mean_circ: 0.941003
Ran for 218050 frames Cell Events: 7360 mean_circ: 0.941059
Ran for 219050 frames Cell Events: 7390 mean_circ: 0.94106
Ran for 219550 frames Cell Events: 7405 mean_circ: 0.941088
Ran for 220050 frames Cell Events: 7429 mean_circ: 0.941091
Ran for 220550 frames Cell Events: 7441 mean_circ: 0.941098
Ran for 221550 frames Cell Events: 7476 mean_circ: 0.941061
Ran for 222050 frames Cell Events: 7484 mean_circ: 0.941057
Ran for 222550 frames Cell Events: 7507 mean_circ: 0.941054
Ran for 223050 frames Cell Events: 7523 mean_circ: 0.941064
Ran for 223550 frames Cell Events: 7539 mean_circ: 0.941062
Ran for 224550 frames Cell Events: 7577 mean_circ: 0.941089
Ran for 225050 frames Cell Events: 7592 mean_circ: 0.941078
Ran for 225550 frames Cell Events: 7605 mean_circ: 0.941092
Ran for 226050 frames Cell Events: 7626 mean_circ: 0.94104
Ran for 226550 frames Cell Events: 7648 mean_circ: 0.94104
Ran for 227050 frames Cell Events: 7675 mean_circ: 0.941011
Ran for 228050 frames Cell Events: 7702 mean_circ: 0.941002
Ran for 228550 frames Cell Events: 7718 mean_circ: 0.941021
Ran for 229050 frames Cell Events: 7731 mean_circ: 0.941003
Ran for 229550 frames Cell Events: 7744 mean_circ: 0.940961
Ran for 231050 frames Cell Events: 7803 mean_circ: 0.940896
Ran for 231550 frames Cell Events: 7817 mean_circ: 0.940901
Ran for 232050 frames Cell Events: 7830 mean_circ: 0.940898
Ran for 232550 frames Cell Events: 7844 mean_circ: 0.940875
Ran for 234050 frames Cell Events: 7902 mean_circ: 0.940836
Ran for 234550 frames Cell Events: 7922 mean_circ: 0.940812
Ran for 235550 frames Cell Events: 7954 mean_circ: 0.940789
Ran for 236550 frames Cell Events: 7987 mean_circ: 0.940784
Ran for 238550 frames Cell Events: 8044 mean_circ: 0.940702
Ran for 239550 frames Cell Events: 8103 mean_circ: 0.940679
Ran for 240050 frames Cell Events: 8138 mean_circ: 0.940663
End this measurment..
Stopping time: 1.33509e+009
****************************************
Measurement finished
Mean Circularitiy: 0.940666
Mean Area: 642.415
Mean FL-1 intensity: 2646.76
Mean FL-2 intensity: 3858.45
Mean FL-3 intensity: 0
frames processed: 237694
Total Time: 120316ms
Number of Runs (new bg img) 0
Frames collected: 240431
Skipped frames: 79282
Rescued Frames: 3680804
Samples DAQ: 60108760
Samples written: 120216274
Samples per Channel: 60108137
FPS: 1998.34
Samples per second: 499587
Expected samples/s: 500000
Events: 8159
Events per second: 67.8133
jumped back (PMT): 5768
#Frames read, frequency
# 0 frames :322819
# 1 frames :141055
# 2 frames :5289
# 3 frames :1560
# 4 frames :607
# 5 frames :234
# 6 frames :3506
# 7 frames :1693
# 8 frames :1384
# 9 frames :544
# 10 frames :497
# 11 frames :268
# 12 frames :356
# 13 frames :169
# 14 frames :230
# 15 frames :150
# 16 frames :116
# 17 frames :84
# 18 frames :54
# 19 frames :45
# 20 frames :37
# 21 frames :30
# 22 frames :17
# 23 frames :21
# 24 frames :15
# 25 frames :11
# 26 frames :10
# 27 frames :6
# 28 frames :3
# 29 frames :1
# 30 frames :1
# 31 frames :4
# 32 frames :2
# 34 frames :1
# 36 frames :3
# 38 frames :1
# 39 frames :1
# 40 frames :1
# 42 frames :1
# 44 frames :1
# 54 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6261
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0