Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_19.04.11
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "4ab00432ec6856e221a79416db6facd9",
"name": "M1_20170521_1741_dsRNA_A2_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:04:33"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8208 (max 8209) in '8208'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0986
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 5.5193e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 9 mean_circ: 0.944506
Ran for 1050 frames Cell Events: 15 mean_circ: 0.944026
Ran for 1550 frames Cell Events: 26 mean_circ: 0.945889
Ran for 3050 frames Cell Events: 68 mean_circ: 0.94154
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Ran for 4050 frames Cell Events: 95 mean_circ: 0.942488
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Ran for 10050 frames Cell Events: 236 mean_circ: 0.939724
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Ran for 11050 frames Cell Events: 273 mean_circ: 0.940584
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Ran for 73050 frames Cell Events: 1949 mean_circ: 0.939637
Ran for 73550 frames Cell Events: 1964 mean_circ: 0.939537
Ran for 74050 frames Cell Events: 1978 mean_circ: 0.939584
Ran for 74550 frames Cell Events: 1984 mean_circ: 0.939616
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Ran for 75550 frames Cell Events: 2005 mean_circ: 0.939625
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Ran for 87550 frames Cell Events: 2343 mean_circ: 0.939919
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Ran for 91550 frames Cell Events: 2478 mean_circ: 0.940095
Ran for 92050 frames Cell Events: 2490 mean_circ: 0.940167
Ran for 92550 frames Cell Events: 2506 mean_circ: 0.940216
Ran for 93550 frames Cell Events: 2532 mean_circ: 0.940046
Ran for 94050 frames Cell Events: 2552 mean_circ: 0.939955
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Ran for 123050 frames Cell Events: 3356 mean_circ: 0.939623
Ran for 123550 frames Cell Events: 3367 mean_circ: 0.939627
Ran for 125550 frames Cell Events: 3421 mean_circ: 0.939544
Ran for 126050 frames Cell Events: 3432 mean_circ: 0.93959
Ran for 126550 frames Cell Events: 3440 mean_circ: 0.939609
Ran for 127050 frames Cell Events: 3451 mean_circ: 0.939604
Ran for 128050 frames Cell Events: 3482 mean_circ: 0.939637
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Ran for 131050 frames Cell Events: 3582 mean_circ: 0.939598
Ran for 131550 frames Cell Events: 3595 mean_circ: 0.939615
Ran for 132050 frames Cell Events: 3607 mean_circ: 0.939665
Ran for 132550 frames Cell Events: 3619 mean_circ: 0.939679
Ran for 133050 frames Cell Events: 3632 mean_circ: 0.9397
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Ran for 138050 frames Cell Events: 3772 mean_circ: 0.939579
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Ran for 182050 frames Cell Events: 5135 mean_circ: 0.939357
Ran for 182550 frames Cell Events: 5158 mean_circ: 0.939317
Ran for 183050 frames Cell Events: 5170 mean_circ: 0.939328
Ran for 183550 frames Cell Events: 5189 mean_circ: 0.93931
Ran for 184550 frames Cell Events: 5210 mean_circ: 0.939336
Ran for 185550 frames Cell Events: 5238 mean_circ: 0.939331
Ran for 186550 frames Cell Events: 5273 mean_circ: 0.939312
Ran for 187050 frames Cell Events: 5290 mean_circ: 0.939317
Ran for 187550 frames Cell Events: 5314 mean_circ: 0.939254
Ran for 188550 frames Cell Events: 5337 mean_circ: 0.939315
Ran for 189050 frames Cell Events: 5345 mean_circ: 0.939288
Ran for 190050 frames Cell Events: 5364 mean_circ: 0.939295
Ran for 191050 frames Cell Events: 5392 mean_circ: 0.939317
Ran for 192050 frames Cell Events: 5419 mean_circ: 0.939301
Ran for 192550 frames Cell Events: 5436 mean_circ: 0.939357
Ran for 193550 frames Cell Events: 5465 mean_circ: 0.939334
Ran for 194050 frames Cell Events: 5486 mean_circ: 0.939338
Ran for 195050 frames Cell Events: 5517 mean_circ: 0.939372
Ran for 195550 frames Cell Events: 5535 mean_circ: 0.939367
Ran for 196050 frames Cell Events: 5555 mean_circ: 0.939367
Ran for 196550 frames Cell Events: 5568 mean_circ: 0.939359
Ran for 197050 frames Cell Events: 5588 mean_circ: 0.939409
Ran for 197550 frames Cell Events: 5610 mean_circ: 0.939354
Ran for 198050 frames Cell Events: 5625 mean_circ: 0.939296
Ran for 200050 frames Cell Events: 5699 mean_circ: 0.939262
Ran for 200550 frames Cell Events: 5719 mean_circ: 0.939274
Ran for 201050 frames Cell Events: 5727 mean_circ: 0.939308
Ran for 201550 frames Cell Events: 5738 mean_circ: 0.939284
Ran for 203550 frames Cell Events: 5811 mean_circ: 0.939218
Ran for 204050 frames Cell Events: 5824 mean_circ: 0.939218
Ran for 205050 frames Cell Events: 5845 mean_circ: 0.93924
Ran for 205550 frames Cell Events: 5863 mean_circ: 0.93921
Ran for 207050 frames Cell Events: 5906 mean_circ: 0.939316
Ran for 208050 frames Cell Events: 5927 mean_circ: 0.93935
Ran for 208550 frames Cell Events: 5947 mean_circ: 0.939359
Ran for 209050 frames Cell Events: 5958 mean_circ: 0.939387
Ran for 209550 frames Cell Events: 5978 mean_circ: 0.939341
Ran for 210550 frames Cell Events: 6009 mean_circ: 0.939321
Ran for 211050 frames Cell Events: 6024 mean_circ: 0.939318
Ran for 212550 frames Cell Events: 6094 mean_circ: 0.939304
Ran for 213050 frames Cell Events: 6102 mean_circ: 0.939291
Ran for 213550 frames Cell Events: 6117 mean_circ: 0.939289
Ran for 214050 frames Cell Events: 6132 mean_circ: 0.939299
Ran for 215050 frames Cell Events: 6164 mean_circ: 0.939305
Ran for 216050 frames Cell Events: 6190 mean_circ: 0.939338
Ran for 216550 frames Cell Events: 6204 mean_circ: 0.93934
Ran for 217050 frames Cell Events: 6219 mean_circ: 0.939326
Ran for 217550 frames Cell Events: 6234 mean_circ: 0.93933
Ran for 218050 frames Cell Events: 6252 mean_circ: 0.939367
Ran for 218550 frames Cell Events: 6266 mean_circ: 0.939366
Ran for 219050 frames Cell Events: 6271 mean_circ: 0.939341
Ran for 219550 frames Cell Events: 6282 mean_circ: 0.939347
Ran for 220050 frames Cell Events: 6299 mean_circ: 0.939321
Ran for 220550 frames Cell Events: 6316 mean_circ: 0.939364
Ran for 221050 frames Cell Events: 6338 mean_circ: 0.93939
Ran for 221550 frames Cell Events: 6356 mean_circ: 0.939437
Ran for 222050 frames Cell Events: 6368 mean_circ: 0.939448
Ran for 223050 frames Cell Events: 6409 mean_circ: 0.939483
Ran for 223550 frames Cell Events: 6415 mean_circ: 0.939515
Ran for 224050 frames Cell Events: 6428 mean_circ: 0.939541
Ran for 225050 frames Cell Events: 6457 mean_circ: 0.939572
Ran for 225550 frames Cell Events: 6467 mean_circ: 0.939609
Ran for 226050 frames Cell Events: 6478 mean_circ: 0.939617
Ran for 227050 frames Cell Events: 6510 mean_circ: 0.939568
Ran for 227550 frames Cell Events: 6530 mean_circ: 0.939571
Ran for 228550 frames Cell Events: 6566 mean_circ: 0.939629
Ran for 229050 frames Cell Events: 6582 mean_circ: 0.939638
Ran for 229550 frames Cell Events: 6595 mean_circ: 0.93964
Ran for 230050 frames Cell Events: 6609 mean_circ: 0.939654
Ran for 231050 frames Cell Events: 6650 mean_circ: 0.939694
Ran for 232050 frames Cell Events: 6674 mean_circ: 0.939758
Ran for 233050 frames Cell Events: 6693 mean_circ: 0.939746
Ran for 233550 frames Cell Events: 6706 mean_circ: 0.939758
Ran for 235550 frames Cell Events: 6763 mean_circ: 0.939786
Ran for 236050 frames Cell Events: 6770 mean_circ: 0.93981
Ran for 237050 frames Cell Events: 6799 mean_circ: 0.939832
Ran for 237550 frames Cell Events: 6806 mean_circ: 0.93983
Ran for 238550 frames Cell Events: 6838 mean_circ: 0.939839
Ran for 239550 frames Cell Events: 6868 mean_circ: 0.939888
Ran for 240050 frames Cell Events: 6886 mean_circ: 0.939919
Ran for 240550 frames Cell Events: 6901 mean_circ: 0.939911
Ran for 241050 frames Cell Events: 6911 mean_circ: 0.939906
Ran for 241550 frames Cell Events: 6923 mean_circ: 0.939908
Ran for 243550 frames Cell Events: 6999 mean_circ: 0.939859
Ran for 244050 frames Cell Events: 7011 mean_circ: 0.939834
Ran for 244550 frames Cell Events: 7033 mean_circ: 0.939872
Ran for 247550 frames Cell Events: 7128 mean_circ: 0.939699
Ran for 248550 frames Cell Events: 7160 mean_circ: 0.939653
Ran for 249550 frames Cell Events: 7191 mean_circ: 0.939682
Ran for 250550 frames Cell Events: 7226 mean_circ: 0.939648
Ran for 251550 frames Cell Events: 7253 mean_circ: 0.939712
Ran for 252050 frames Cell Events: 7278 mean_circ: 0.939717
Ran for 252550 frames Cell Events: 7293 mean_circ: 0.93973
Ran for 253050 frames Cell Events: 7308 mean_circ: 0.939727
Ran for 254050 frames Cell Events: 7331 mean_circ: 0.939708
Ran for 254550 frames Cell Events: 7341 mean_circ: 0.939723
Ran for 255050 frames Cell Events: 7351 mean_circ: 0.939698
Ran for 255550 frames Cell Events: 7364 mean_circ: 0.93972
Ran for 256050 frames Cell Events: 7373 mean_circ: 0.939712
Ran for 256550 frames Cell Events: 7383 mean_circ: 0.939718
Ran for 257050 frames Cell Events: 7409 mean_circ: 0.939718
Ran for 257550 frames Cell Events: 7426 mean_circ: 0.939712
Ran for 258050 frames Cell Events: 7440 mean_circ: 0.939701
Ran for 259550 frames Cell Events: 7495 mean_circ: 0.939708
Ran for 260050 frames Cell Events: 7506 mean_circ: 0.9397
Ran for 260550 frames Cell Events: 7524 mean_circ: 0.939676
Ran for 261050 frames Cell Events: 7547 mean_circ: 0.939668
Ran for 262550 frames Cell Events: 7606 mean_circ: 0.939679
Ran for 263050 frames Cell Events: 7623 mean_circ: 0.939623
Ran for 263550 frames Cell Events: 7633 mean_circ: 0.939601
Ran for 264050 frames Cell Events: 7644 mean_circ: 0.939611
Ran for 264550 frames Cell Events: 7667 mean_circ: 0.939606
Ran for 265050 frames Cell Events: 7684 mean_circ: 0.939598
Ran for 265550 frames Cell Events: 7699 mean_circ: 0.939604
Ran for 266050 frames Cell Events: 7713 mean_circ: 0.939636
Ran for 266550 frames Cell Events: 7720 mean_circ: 0.939612
Ran for 267050 frames Cell Events: 7740 mean_circ: 0.939633
Ran for 268050 frames Cell Events: 7783 mean_circ: 0.939694
Ran for 268550 frames Cell Events: 7801 mean_circ: 0.939701
Ran for 269050 frames Cell Events: 7816 mean_circ: 0.939673
Ran for 269550 frames Cell Events: 7831 mean_circ: 0.9397
Ran for 270050 frames Cell Events: 7845 mean_circ: 0.939708
Ran for 271050 frames Cell Events: 7880 mean_circ: 0.939613
Ran for 272550 frames Cell Events: 7938 mean_circ: 0.939597
Ran for 273050 frames Cell Events: 7954 mean_circ: 0.939596
Ran for 273550 frames Cell Events: 7974 mean_circ: 0.939554
Ran for 274050 frames Cell Events: 7998 mean_circ: 0.939549
Ran for 275550 frames Cell Events: 8052 mean_circ: 0.939547
Ran for 276050 frames Cell Events: 8082 mean_circ: 0.939556
Ran for 276550 frames Cell Events: 8097 mean_circ: 0.939516
Ran for 277050 frames Cell Events: 8108 mean_circ: 0.939475
Ran for 277550 frames Cell Events: 8119 mean_circ: 0.939505
Ran for 278550 frames Cell Events: 8153 mean_circ: 0.939546
Ran for 279050 frames Cell Events: 8174 mean_circ: 0.939549
End this measurment..
Stopping time: 5.5207e+008
****************************************
Measurement finished
Mean Circularitiy: 0.93955
Mean Area: 652.673
Mean FL-1 intensity: 3089.03
Mean FL-2 intensity: 3960.3
Mean FL-3 intensity: 0
frames processed: 280094
Total Time: 140218ms
Number of Runs (new bg img) 0
Frames collected: 280259
Skipped frames: 56715
Rescued Frames: 2805558
Samples DAQ: 70064852
Samples written: 140129610
Samples per Channel: 70064805
FPS: 1998.74
Samples per second: 499686
Expected samples/s: 500000
Events: 8209
Events per second: 58.5446
jumped back (PMT): 4891
#Frames read, frequency
# 0 frames :391338
# 1 frames :150233
# 2 frames :5116
# 3 frames :2051
# 4 frames :1276
# 5 frames :584
# 6 frames :972
# 7 frames :2120
# 8 frames :1166
# 9 frames :798
# 10 frames :868
# 11 frames :840
# 12 frames :648
# 13 frames :493
# 14 frames :326
# 15 frames :300
# 16 frames :240
# 17 frames :210
# 18 frames :205
# 19 frames :136
# 20 frames :119
# 21 frames :68
# 22 frames :57
# 23 frames :46
# 24 frames :42
# 25 frames :34
# 26 frames :27
# 27 frames :20
# 28 frames :31
# 29 frames :12
# 30 frames :23
# 31 frames :15
# 32 frames :12
# 33 frames :7
# 34 frames :7
# 35 frames :6
# 36 frames :3
# 37 frames :3
# 38 frames :4
# 39 frames :1
# 40 frames :1
# 41 frames :1
# 42 frames :1
# 43 frames :1
# 45 frames :2
# 48 frames :1
# 49 frames :1
# 51 frames :1
# 53 frames :1
# 61 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0986
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0