Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_19.27.58
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "3de73373811e920259924604aff0ad04",
"name": "M1_20170522_1519_dsRNA_D2_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:28:22"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9090 (max 9091) in '9090'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.0572
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 6.29817e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 24 mean_circ: 0.946796
Ran for 1050 frames Cell Events: 46 mean_circ: 0.950566
Ran for 1550 frames Cell Events: 71 mean_circ: 0.94784
Ran for 2050 frames Cell Events: 95 mean_circ: 0.947642
Ran for 2550 frames Cell Events: 108 mean_circ: 0.949377
Ran for 3050 frames Cell Events: 126 mean_circ: 0.945514
Ran for 3550 frames Cell Events: 149 mean_circ: 0.945152
Ran for 4550 frames Cell Events: 195 mean_circ: 0.945844
Ran for 5050 frames Cell Events: 216 mean_circ: 0.946499
Ran for 5550 frames Cell Events: 245 mean_circ: 0.947671
Ran for 7050 frames Cell Events: 322 mean_circ: 0.948073
Ran for 7550 frames Cell Events: 338 mean_circ: 0.948284
Ran for 8050 frames Cell Events: 355 mean_circ: 0.948269
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Ran for 10050 frames Cell Events: 434 mean_circ: 0.948673
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Ran for 11550 frames Cell Events: 492 mean_circ: 0.947132
Ran for 12550 frames Cell Events: 525 mean_circ: 0.946968
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Ran for 16050 frames Cell Events: 647 mean_circ: 0.946745
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Ran for 69050 frames Cell Events: 2725 mean_circ: 0.94858
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Ran for 72550 frames Cell Events: 2862 mean_circ: 0.948853
Ran for 73050 frames Cell Events: 2883 mean_circ: 0.948837
Ran for 74050 frames Cell Events: 2910 mean_circ: 0.948844
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Ran for 76050 frames Cell Events: 2993 mean_circ: 0.948927
Ran for 76550 frames Cell Events: 3029 mean_circ: 0.948808
Ran for 77550 frames Cell Events: 3075 mean_circ: 0.948715
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Ran for 81550 frames Cell Events: 3230 mean_circ: 0.948936
Ran for 82050 frames Cell Events: 3249 mean_circ: 0.948932
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Ran for 83550 frames Cell Events: 3305 mean_circ: 0.948844
Ran for 84050 frames Cell Events: 3324 mean_circ: 0.948778
Ran for 85050 frames Cell Events: 3367 mean_circ: 0.948738
Ran for 86050 frames Cell Events: 3410 mean_circ: 0.948673
Ran for 86550 frames Cell Events: 3426 mean_circ: 0.948689
Ran for 87050 frames Cell Events: 3444 mean_circ: 0.948685
Ran for 87550 frames Cell Events: 3473 mean_circ: 0.948671
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Ran for 88550 frames Cell Events: 3519 mean_circ: 0.948603
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Ran for 89550 frames Cell Events: 3551 mean_circ: 0.94864
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Ran for 91050 frames Cell Events: 3615 mean_circ: 0.948556
Ran for 92050 frames Cell Events: 3664 mean_circ: 0.948559
Ran for 92550 frames Cell Events: 3683 mean_circ: 0.94861
Ran for 93050 frames Cell Events: 3700 mean_circ: 0.948637
Ran for 95050 frames Cell Events: 3797 mean_circ: 0.948674
Ran for 95550 frames Cell Events: 3821 mean_circ: 0.948748
Ran for 96050 frames Cell Events: 3839 mean_circ: 0.948745
Ran for 97050 frames Cell Events: 3897 mean_circ: 0.94881
Ran for 98550 frames Cell Events: 3977 mean_circ: 0.948722
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Ran for 99550 frames Cell Events: 4027 mean_circ: 0.948656
Ran for 102050 frames Cell Events: 4150 mean_circ: 0.948592
Ran for 102550 frames Cell Events: 4179 mean_circ: 0.948648
Ran for 103050 frames Cell Events: 4201 mean_circ: 0.948687
Ran for 103550 frames Cell Events: 4229 mean_circ: 0.948647
Ran for 104550 frames Cell Events: 4273 mean_circ: 0.948553
Ran for 105550 frames Cell Events: 4320 mean_circ: 0.948536
Ran for 106050 frames Cell Events: 4335 mean_circ: 0.948578
Ran for 106550 frames Cell Events: 4343 mean_circ: 0.948566
Ran for 107050 frames Cell Events: 4358 mean_circ: 0.94857
Ran for 107550 frames Cell Events: 4376 mean_circ: 0.948577
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Ran for 119050 frames Cell Events: 4880 mean_circ: 0.948531
Ran for 120050 frames Cell Events: 4925 mean_circ: 0.948609
Ran for 121050 frames Cell Events: 4981 mean_circ: 0.948616
Ran for 122050 frames Cell Events: 5035 mean_circ: 0.948661
Ran for 123550 frames Cell Events: 5108 mean_circ: 0.94873
Ran for 124050 frames Cell Events: 5121 mean_circ: 0.948736
Ran for 125050 frames Cell Events: 5158 mean_circ: 0.948745
Ran for 126550 frames Cell Events: 5223 mean_circ: 0.948785
Ran for 127550 frames Cell Events: 5257 mean_circ: 0.948776
Ran for 128050 frames Cell Events: 5280 mean_circ: 0.948763
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Ran for 129050 frames Cell Events: 5320 mean_circ: 0.948757
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Ran for 130550 frames Cell Events: 5393 mean_circ: 0.948829
Ran for 131050 frames Cell Events: 5415 mean_circ: 0.948853
Ran for 131550 frames Cell Events: 5435 mean_circ: 0.948837
Ran for 132050 frames Cell Events: 5453 mean_circ: 0.948833
Ran for 132550 frames Cell Events: 5472 mean_circ: 0.948808
Ran for 133050 frames Cell Events: 5493 mean_circ: 0.948794
Ran for 133550 frames Cell Events: 5525 mean_circ: 0.948685
Ran for 134050 frames Cell Events: 5546 mean_circ: 0.948665
Ran for 134550 frames Cell Events: 5573 mean_circ: 0.948669
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Ran for 141550 frames Cell Events: 5890 mean_circ: 0.948666
Ran for 142050 frames Cell Events: 5910 mean_circ: 0.94873
Ran for 142550 frames Cell Events: 5930 mean_circ: 0.948608
Ran for 144050 frames Cell Events: 5989 mean_circ: 0.948602
Ran for 145050 frames Cell Events: 6050 mean_circ: 0.948626
Ran for 146050 frames Cell Events: 6092 mean_circ: 0.94863
Ran for 146550 frames Cell Events: 6107 mean_circ: 0.948594
Ran for 147050 frames Cell Events: 6129 mean_circ: 0.948589
Ran for 147550 frames Cell Events: 6149 mean_circ: 0.948599
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Ran for 149050 frames Cell Events: 6210 mean_circ: 0.94865
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Ran for 206050 frames Cell Events: 8785 mean_circ: 0.948597
Ran for 206550 frames Cell Events: 8804 mean_circ: 0.948595
Ran for 207550 frames Cell Events: 8843 mean_circ: 0.948557
Ran for 208050 frames Cell Events: 8858 mean_circ: 0.948568
Ran for 209550 frames Cell Events: 8929 mean_circ: 0.948525
Ran for 210050 frames Cell Events: 8939 mean_circ: 0.94853
Ran for 210550 frames Cell Events: 8962 mean_circ: 0.948525
Ran for 211050 frames Cell Events: 8978 mean_circ: 0.948504
Ran for 211550 frames Cell Events: 9002 mean_circ: 0.948524
Ran for 212550 frames Cell Events: 9047 mean_circ: 0.948572
Ran for 213550 frames Cell Events: 9084 mean_circ: 0.948572
End this measurment..
Stopping time: 6.29924e+008
****************************************
Measurement finished
Mean Circularitiy: 0.948569
Mean Area: 588.305
Mean FL-1 intensity: 3337.22
Mean FL-2 intensity: 4636.85
Mean FL-3 intensity: 0
frames processed: 213696
Total Time: 106941ms
Number of Runs (new bg img) 0
Frames collected: 213754
Skipped frames: 39808
Rescued Frames: 2006626
Samples DAQ: 53438635
Samples written: 106877028
Samples per Channel: 53438514
FPS: 1998.79
Samples per second: 499699
Expected samples/s: 500000
Events: 9091
Events per second: 85.0091
jumped back (PMT): 5212
#Frames read, frequency
# 0 frames :304241
# 1 frames :106409
# 2 frames :4765
# 3 frames :1650
# 4 frames :757
# 5 frames :291
# 6 frames :1790
# 7 frames :2146
# 8 frames :1100
# 9 frames :613
# 10 frames :1377
# 11 frames :576
# 12 frames :377
# 13 frames :219
# 14 frames :209
# 15 frames :195
# 16 frames :145
# 17 frames :113
# 18 frames :92
# 19 frames :42
# 20 frames :36
# 21 frames :61
# 22 frames :29
# 23 frames :34
# 24 frames :31
# 25 frames :24
# 26 frames :14
# 27 frames :10
# 28 frames :14
# 29 frames :15
# 30 frames :12
# 31 frames :12
# 32 frames :9
# 33 frames :3
# 34 frames :3
# 35 frames :5
# 36 frames :3
# 37 frames :3
# 39 frames :2
# 40 frames :2
# 42 frames :1
# 43 frames :1
# 44 frames :1
# 46 frames :1
# 48 frames :1
# 58 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.0572
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0