Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_19.29.05
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "8ba81b41d95fdfb069a095812b455cdf",
"name": "M1_20170522_1531_dsRNA_D6_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:29:26"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8045 (max 8046) in '8045'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.9902
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 6.30523e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 25 mean_circ: 0.930831
Ran for 1050 frames Cell Events: 51 mean_circ: 0.945265
Ran for 2050 frames Cell Events: 97 mean_circ: 0.948575
Ran for 2550 frames Cell Events: 113 mean_circ: 0.946802
Ran for 3050 frames Cell Events: 139 mean_circ: 0.948252
Ran for 3550 frames Cell Events: 164 mean_circ: 0.94928
Ran for 4050 frames Cell Events: 189 mean_circ: 0.949233
Ran for 5050 frames Cell Events: 221 mean_circ: 0.950189
Ran for 6050 frames Cell Events: 272 mean_circ: 0.948358
Ran for 6550 frames Cell Events: 298 mean_circ: 0.948136
Ran for 7050 frames Cell Events: 323 mean_circ: 0.948375
Ran for 9050 frames Cell Events: 443 mean_circ: 0.948371
Ran for 9550 frames Cell Events: 458 mean_circ: 0.948845
Ran for 10050 frames Cell Events: 489 mean_circ: 0.948752
Ran for 10550 frames Cell Events: 511 mean_circ: 0.948914
Ran for 11050 frames Cell Events: 534 mean_circ: 0.948403
Ran for 13050 frames Cell Events: 661 mean_circ: 0.946925
Ran for 13550 frames Cell Events: 689 mean_circ: 0.946398
Ran for 15550 frames Cell Events: 824 mean_circ: 0.946244
Ran for 16050 frames Cell Events: 870 mean_circ: 0.946244
Ran for 16550 frames Cell Events: 893 mean_circ: 0.94572
Ran for 17050 frames Cell Events: 926 mean_circ: 0.946106
Ran for 17550 frames Cell Events: 945 mean_circ: 0.946099
Ran for 18050 frames Cell Events: 976 mean_circ: 0.946125
Ran for 18550 frames Cell Events: 1009 mean_circ: 0.946154
Ran for 19050 frames Cell Events: 1051 mean_circ: 0.945714
Ran for 20550 frames Cell Events: 1144 mean_circ: 0.946217
Ran for 22550 frames Cell Events: 1260 mean_circ: 0.946173
Ran for 23550 frames Cell Events: 1328 mean_circ: 0.946342
Ran for 24050 frames Cell Events: 1351 mean_circ: 0.946302
Ran for 24550 frames Cell Events: 1374 mean_circ: 0.946406
Ran for 25050 frames Cell Events: 1401 mean_circ: 0.946428
Ran for 25550 frames Cell Events: 1424 mean_circ: 0.946528
Ran for 26050 frames Cell Events: 1440 mean_circ: 0.946354
Ran for 26550 frames Cell Events: 1477 mean_circ: 0.946243
Ran for 28050 frames Cell Events: 1560 mean_circ: 0.946645
Ran for 28550 frames Cell Events: 1590 mean_circ: 0.946604
Ran for 29050 frames Cell Events: 1624 mean_circ: 0.946421
Ran for 30050 frames Cell Events: 1697 mean_circ: 0.946568
Ran for 30550 frames Cell Events: 1724 mean_circ: 0.946705
Ran for 31550 frames Cell Events: 1771 mean_circ: 0.947052
Ran for 32050 frames Cell Events: 1791 mean_circ: 0.947061
Ran for 32550 frames Cell Events: 1807 mean_circ: 0.946996
Ran for 33050 frames Cell Events: 1832 mean_circ: 0.947046
Ran for 34050 frames Cell Events: 1873 mean_circ: 0.947007
Ran for 34550 frames Cell Events: 1910 mean_circ: 0.946996
Ran for 35550 frames Cell Events: 1969 mean_circ: 0.94675
Ran for 36050 frames Cell Events: 1989 mean_circ: 0.946824
Ran for 36550 frames Cell Events: 2024 mean_circ: 0.946864
Ran for 37050 frames Cell Events: 2042 mean_circ: 0.946955
Ran for 38050 frames Cell Events: 2121 mean_circ: 0.946548
Ran for 38550 frames Cell Events: 2148 mean_circ: 0.946645
Ran for 39050 frames Cell Events: 2191 mean_circ: 0.946761
Ran for 42050 frames Cell Events: 2395 mean_circ: 0.946346
Ran for 43050 frames Cell Events: 2453 mean_circ: 0.946447
Ran for 43550 frames Cell Events: 2475 mean_circ: 0.946617
Ran for 44050 frames Cell Events: 2498 mean_circ: 0.946598
Ran for 44550 frames Cell Events: 2523 mean_circ: 0.946575
Ran for 45550 frames Cell Events: 2586 mean_circ: 0.946563
Ran for 46050 frames Cell Events: 2602 mean_circ: 0.946665
Ran for 46550 frames Cell Events: 2623 mean_circ: 0.946757
Ran for 47050 frames Cell Events: 2649 mean_circ: 0.946809
Ran for 47550 frames Cell Events: 2676 mean_circ: 0.946902
Ran for 48050 frames Cell Events: 2691 mean_circ: 0.946963
Ran for 49550 frames Cell Events: 2759 mean_circ: 0.946972
Ran for 50050 frames Cell Events: 2779 mean_circ: 0.946809
Ran for 51050 frames Cell Events: 2834 mean_circ: 0.946946
Ran for 51550 frames Cell Events: 2858 mean_circ: 0.946929
Ran for 52050 frames Cell Events: 2886 mean_circ: 0.946743
Ran for 52550 frames Cell Events: 2912 mean_circ: 0.946781
Ran for 53550 frames Cell Events: 2961 mean_circ: 0.946769
Ran for 54550 frames Cell Events: 3022 mean_circ: 0.946665
Ran for 55550 frames Cell Events: 3084 mean_circ: 0.946629
Ran for 56050 frames Cell Events: 3122 mean_circ: 0.946533
Ran for 56550 frames Cell Events: 3146 mean_circ: 0.94637
Ran for 57550 frames Cell Events: 3197 mean_circ: 0.946317
Ran for 58050 frames Cell Events: 3231 mean_circ: 0.946271
Ran for 62050 frames Cell Events: 3473 mean_circ: 0.946516
Ran for 64050 frames Cell Events: 3578 mean_circ: 0.946467
Ran for 64550 frames Cell Events: 3608 mean_circ: 0.946414
Ran for 65050 frames Cell Events: 3644 mean_circ: 0.946463
Ran for 66050 frames Cell Events: 3690 mean_circ: 0.946483
Ran for 66550 frames Cell Events: 3708 mean_circ: 0.946521
Ran for 67050 frames Cell Events: 3718 mean_circ: 0.946523
Ran for 67550 frames Cell Events: 3736 mean_circ: 0.946609
Ran for 68050 frames Cell Events: 3769 mean_circ: 0.946632
Ran for 68550 frames Cell Events: 3794 mean_circ: 0.94658
Ran for 69550 frames Cell Events: 3842 mean_circ: 0.946661
Ran for 70550 frames Cell Events: 3913 mean_circ: 0.946547
Ran for 71050 frames Cell Events: 3945 mean_circ: 0.946544
Ran for 72050 frames Cell Events: 3994 mean_circ: 0.946535
Ran for 72550 frames Cell Events: 4023 mean_circ: 0.946582
Ran for 73550 frames Cell Events: 4071 mean_circ: 0.94657
Ran for 74550 frames Cell Events: 4134 mean_circ: 0.94657
Ran for 75050 frames Cell Events: 4139 mean_circ: 0.946509
Ran for 76050 frames Cell Events: 4198 mean_circ: 0.946487
Ran for 76550 frames Cell Events: 4223 mean_circ: 0.946497
Ran for 77050 frames Cell Events: 4250 mean_circ: 0.946499
Ran for 77550 frames Cell Events: 4278 mean_circ: 0.946382
Ran for 78550 frames Cell Events: 4315 mean_circ: 0.946389
Ran for 79050 frames Cell Events: 4344 mean_circ: 0.946377
Ran for 79550 frames Cell Events: 4364 mean_circ: 0.946259
Ran for 80550 frames Cell Events: 4441 mean_circ: 0.946204
Ran for 81050 frames Cell Events: 4464 mean_circ: 0.946128
Ran for 81550 frames Cell Events: 4501 mean_circ: 0.946122
Ran for 82550 frames Cell Events: 4563 mean_circ: 0.946114
Ran for 83050 frames Cell Events: 4588 mean_circ: 0.94617
Ran for 85550 frames Cell Events: 4708 mean_circ: 0.946202
Ran for 86050 frames Cell Events: 4729 mean_circ: 0.946178
Ran for 86550 frames Cell Events: 4750 mean_circ: 0.946134
Ran for 87050 frames Cell Events: 4762 mean_circ: 0.94611
Ran for 88050 frames Cell Events: 4803 mean_circ: 0.946207
Ran for 88550 frames Cell Events: 4833 mean_circ: 0.946158
Ran for 89050 frames Cell Events: 4863 mean_circ: 0.946171
Ran for 89550 frames Cell Events: 4883 mean_circ: 0.946223
Ran for 90050 frames Cell Events: 4909 mean_circ: 0.946242
Ran for 90550 frames Cell Events: 4928 mean_circ: 0.946248
Ran for 91050 frames Cell Events: 4953 mean_circ: 0.94622
Ran for 94050 frames Cell Events: 5141 mean_circ: 0.946053
Ran for 94550 frames Cell Events: 5174 mean_circ: 0.946058
Ran for 95050 frames Cell Events: 5207 mean_circ: 0.946024
Ran for 96050 frames Cell Events: 5250 mean_circ: 0.946038
Ran for 96550 frames Cell Events: 5274 mean_circ: 0.946006
Ran for 97050 frames Cell Events: 5306 mean_circ: 0.945992
Ran for 99050 frames Cell Events: 5396 mean_circ: 0.946015
Ran for 99550 frames Cell Events: 5421 mean_circ: 0.94607
Ran for 100050 frames Cell Events: 5451 mean_circ: 0.946058
Ran for 100550 frames Cell Events: 5478 mean_circ: 0.94601
Ran for 101050 frames Cell Events: 5502 mean_circ: 0.946002
Ran for 101550 frames Cell Events: 5535 mean_circ: 0.945967
Ran for 102550 frames Cell Events: 5579 mean_circ: 0.945971
Ran for 103550 frames Cell Events: 5633 mean_circ: 0.946029
Ran for 104550 frames Cell Events: 5690 mean_circ: 0.946137
Ran for 106550 frames Cell Events: 5815 mean_circ: 0.946125
Ran for 109550 frames Cell Events: 5979 mean_circ: 0.946065
Ran for 110050 frames Cell Events: 6012 mean_circ: 0.946109
Ran for 111550 frames Cell Events: 6119 mean_circ: 0.946033
Ran for 114050 frames Cell Events: 6262 mean_circ: 0.94595
Ran for 114550 frames Cell Events: 6284 mean_circ: 0.945939
Ran for 115050 frames Cell Events: 6311 mean_circ: 0.945921
Ran for 116050 frames Cell Events: 6375 mean_circ: 0.945851
Ran for 118050 frames Cell Events: 6516 mean_circ: 0.945785
Ran for 118550 frames Cell Events: 6543 mean_circ: 0.945745
Ran for 119050 frames Cell Events: 6573 mean_circ: 0.945765
Ran for 119550 frames Cell Events: 6599 mean_circ: 0.945764
Ran for 120550 frames Cell Events: 6659 mean_circ: 0.945767
Ran for 121550 frames Cell Events: 6706 mean_circ: 0.945816
Ran for 122050 frames Cell Events: 6730 mean_circ: 0.945835
Ran for 122550 frames Cell Events: 6767 mean_circ: 0.94586
Ran for 123050 frames Cell Events: 6817 mean_circ: 0.94586
Ran for 125550 frames Cell Events: 6951 mean_circ: 0.945962
Ran for 126050 frames Cell Events: 6984 mean_circ: 0.945962
Ran for 126550 frames Cell Events: 7003 mean_circ: 0.945998
Ran for 128550 frames Cell Events: 7095 mean_circ: 0.946022
Ran for 131050 frames Cell Events: 7244 mean_circ: 0.946085
Ran for 131550 frames Cell Events: 7265 mean_circ: 0.946091
Ran for 132050 frames Cell Events: 7300 mean_circ: 0.946123
Ran for 132550 frames Cell Events: 7321 mean_circ: 0.94611
Ran for 133050 frames Cell Events: 7355 mean_circ: 0.946089
Ran for 133550 frames Cell Events: 7371 mean_circ: 0.946115
Ran for 134550 frames Cell Events: 7427 mean_circ: 0.946128
Ran for 136050 frames Cell Events: 7516 mean_circ: 0.946134
Ran for 138050 frames Cell Events: 7623 mean_circ: 0.946114
Ran for 139050 frames Cell Events: 7689 mean_circ: 0.946181
Ran for 139550 frames Cell Events: 7714 mean_circ: 0.946165
Ran for 141050 frames Cell Events: 7787 mean_circ: 0.946141
Ran for 143050 frames Cell Events: 7930 mean_circ: 0.946141
Ran for 143550 frames Cell Events: 7948 mean_circ: 0.946152
Ran for 144550 frames Cell Events: 8008 mean_circ: 0.946186
End this measurment..
Stopping time: 6.30596e+008
****************************************
Measurement finished
Mean Circularitiy: 0.946186
Mean Area: 590.499
Mean FL-1 intensity: 3389.22
Mean FL-2 intensity: 4679.32
Mean FL-3 intensity: 0
frames processed: 144651
Total Time: 72726.9ms
Number of Runs (new bg img) 0
Frames collected: 145340
Skipped frames: 42153
Rescued Frames: 2168031
Samples DAQ: 36334958
Samples written: 72669818
Samples per Channel: 36334909
FPS: 1998.44
Samples per second: 499608
Expected samples/s: 500000
Events: 8046
Events per second: 110.633
jumped back (PMT): 3397
#Frames read, frequency
# 0 frames :221529
# 1 frames :57130
# 2 frames :3052
# 3 frames :1000
# 4 frames :674
# 5 frames :216
# 6 frames :1129
# 7 frames :1606
# 8 frames :739
# 9 frames :490
# 10 frames :921
# 11 frames :324
# 12 frames :275
# 13 frames :175
# 14 frames :167
# 15 frames :226
# 16 frames :174
# 17 frames :136
# 18 frames :92
# 19 frames :70
# 20 frames :47
# 21 frames :77
# 22 frames :53
# 23 frames :55
# 24 frames :33
# 25 frames :34
# 26 frames :27
# 27 frames :24
# 28 frames :26
# 29 frames :30
# 30 frames :11
# 31 frames :14
# 32 frames :7
# 33 frames :9
# 34 frames :10
# 35 frames :3
# 36 frames :13
# 37 frames :8
# 38 frames :9
# 39 frames :6
# 40 frames :4
# 41 frames :4
# 42 frames :7
# 43 frames :3
# 44 frames :2
# 45 frames :3
# 46 frames :3
# 47 frames :2
# 48 frames :1
# 49 frames :4
# 50 frames :1
# 51 frames :2
# 52 frames :1
# 53 frames :1
# 55 frames :1
# 56 frames :1
# 57 frames :1
# 59 frames :2
# 60 frames :1
# 63 frames :1
# 73 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.9902
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0