Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_19.24.55
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "e40e597302892f990075bbaaf198c54d",
"name": "M1_20170522_1447_dsRNA_D7_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:25:17"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8906 (max 8907) in '8906'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.3277
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 6.27902e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 24 mean_circ: 0.965131
Ran for 1550 frames Cell Events: 72 mean_circ: 0.949811
Ran for 2050 frames Cell Events: 96 mean_circ: 0.94702
Ran for 3550 frames Cell Events: 165 mean_circ: 0.949447
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Ran for 6550 frames Cell Events: 284 mean_circ: 0.947487
Ran for 7550 frames Cell Events: 322 mean_circ: 0.948125
Ran for 8050 frames Cell Events: 341 mean_circ: 0.948418
Ran for 9550 frames Cell Events: 408 mean_circ: 0.949036
Ran for 10550 frames Cell Events: 455 mean_circ: 0.948347
Ran for 12050 frames Cell Events: 487 mean_circ: 0.948107
Ran for 13050 frames Cell Events: 541 mean_circ: 0.948638
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Ran for 16050 frames Cell Events: 650 mean_circ: 0.948836
Ran for 17050 frames Cell Events: 695 mean_circ: 0.948862
Ran for 18050 frames Cell Events: 743 mean_circ: 0.94905
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Ran for 19550 frames Cell Events: 818 mean_circ: 0.9491
Ran for 20050 frames Cell Events: 840 mean_circ: 0.949122
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Ran for 68550 frames Cell Events: 2792 mean_circ: 0.949373
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Ran for 70550 frames Cell Events: 2879 mean_circ: 0.949262
Ran for 71550 frames Cell Events: 2927 mean_circ: 0.949345
Ran for 73050 frames Cell Events: 3009 mean_circ: 0.94921
Ran for 74050 frames Cell Events: 3071 mean_circ: 0.949279
Ran for 75050 frames Cell Events: 3112 mean_circ: 0.949301
Ran for 75550 frames Cell Events: 3121 mean_circ: 0.949277
Ran for 76550 frames Cell Events: 3166 mean_circ: 0.949169
Ran for 77050 frames Cell Events: 3187 mean_circ: 0.949166
Ran for 77550 frames Cell Events: 3210 mean_circ: 0.949266
Ran for 78050 frames Cell Events: 3242 mean_circ: 0.949178
Ran for 78550 frames Cell Events: 3263 mean_circ: 0.949122
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Ran for 80550 frames Cell Events: 3336 mean_circ: 0.949206
Ran for 81550 frames Cell Events: 3384 mean_circ: 0.949214
Ran for 82050 frames Cell Events: 3412 mean_circ: 0.949189
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Ran for 83050 frames Cell Events: 3471 mean_circ: 0.94923
Ran for 83550 frames Cell Events: 3495 mean_circ: 0.949267
Ran for 84050 frames Cell Events: 3522 mean_circ: 0.949307
Ran for 85550 frames Cell Events: 3597 mean_circ: 0.949388
Ran for 86050 frames Cell Events: 3628 mean_circ: 0.949367
Ran for 87050 frames Cell Events: 3677 mean_circ: 0.949375
Ran for 87550 frames Cell Events: 3697 mean_circ: 0.949404
Ran for 88050 frames Cell Events: 3717 mean_circ: 0.949404
Ran for 88550 frames Cell Events: 3734 mean_circ: 0.949308
Ran for 89050 frames Cell Events: 3757 mean_circ: 0.949318
Ran for 89550 frames Cell Events: 3774 mean_circ: 0.949221
Ran for 90050 frames Cell Events: 3793 mean_circ: 0.949248
Ran for 90550 frames Cell Events: 3813 mean_circ: 0.949249
Ran for 91050 frames Cell Events: 3838 mean_circ: 0.949195
Ran for 91550 frames Cell Events: 3852 mean_circ: 0.949161
Ran for 92050 frames Cell Events: 3884 mean_circ: 0.949159
Ran for 92550 frames Cell Events: 3919 mean_circ: 0.949205
Ran for 93050 frames Cell Events: 3934 mean_circ: 0.949143
Ran for 93550 frames Cell Events: 3949 mean_circ: 0.949144
Ran for 94050 frames Cell Events: 3965 mean_circ: 0.949147
Ran for 94550 frames Cell Events: 3985 mean_circ: 0.949181
Ran for 96050 frames Cell Events: 4053 mean_circ: 0.949003
Ran for 97050 frames Cell Events: 4103 mean_circ: 0.949013
Ran for 97550 frames Cell Events: 4131 mean_circ: 0.949024
Ran for 98050 frames Cell Events: 4156 mean_circ: 0.949072
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Ran for 100550 frames Cell Events: 4274 mean_circ: 0.949159
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Ran for 102050 frames Cell Events: 4355 mean_circ: 0.949116
Ran for 103050 frames Cell Events: 4401 mean_circ: 0.949062
Ran for 103550 frames Cell Events: 4425 mean_circ: 0.949035
Ran for 104050 frames Cell Events: 4446 mean_circ: 0.949015
Ran for 106550 frames Cell Events: 4515 mean_circ: 0.948959
Ran for 107050 frames Cell Events: 4544 mean_circ: 0.948973
Ran for 107550 frames Cell Events: 4581 mean_circ: 0.94896
Ran for 109050 frames Cell Events: 4664 mean_circ: 0.949069
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Ran for 112550 frames Cell Events: 4807 mean_circ: 0.949154
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Ran for 122550 frames Cell Events: 5290 mean_circ: 0.948992
Ran for 124550 frames Cell Events: 5371 mean_circ: 0.94908
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Ran for 127050 frames Cell Events: 5510 mean_circ: 0.948982
Ran for 127550 frames Cell Events: 5523 mean_circ: 0.949007
Ran for 128050 frames Cell Events: 5537 mean_circ: 0.949034
Ran for 129050 frames Cell Events: 5583 mean_circ: 0.949078
Ran for 129550 frames Cell Events: 5600 mean_circ: 0.949125
Ran for 130050 frames Cell Events: 5627 mean_circ: 0.949172
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Ran for 131050 frames Cell Events: 5655 mean_circ: 0.949198
Ran for 131550 frames Cell Events: 5677 mean_circ: 0.949206
Ran for 132050 frames Cell Events: 5703 mean_circ: 0.94924
Ran for 132550 frames Cell Events: 5716 mean_circ: 0.949286
Ran for 133050 frames Cell Events: 5734 mean_circ: 0.949317
Ran for 134550 frames Cell Events: 5799 mean_circ: 0.94934
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Ran for 138050 frames Cell Events: 5935 mean_circ: 0.949252
Ran for 138550 frames Cell Events: 5954 mean_circ: 0.949276
Ran for 139050 frames Cell Events: 5973 mean_circ: 0.949223
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Ran for 141050 frames Cell Events: 6064 mean_circ: 0.949052
Ran for 141550 frames Cell Events: 6092 mean_circ: 0.949015
Ran for 142050 frames Cell Events: 6119 mean_circ: 0.948982
Ran for 142550 frames Cell Events: 6146 mean_circ: 0.948861
Ran for 143550 frames Cell Events: 6194 mean_circ: 0.948937
Ran for 144050 frames Cell Events: 6209 mean_circ: 0.948931
Ran for 144550 frames Cell Events: 6227 mean_circ: 0.948953
Ran for 145050 frames Cell Events: 6255 mean_circ: 0.948998
Ran for 145550 frames Cell Events: 6278 mean_circ: 0.948983
Ran for 146050 frames Cell Events: 6305 mean_circ: 0.948964
Ran for 146550 frames Cell Events: 6324 mean_circ: 0.948936
Ran for 147050 frames Cell Events: 6333 mean_circ: 0.948937
Ran for 147550 frames Cell Events: 6363 mean_circ: 0.94893
Ran for 148050 frames Cell Events: 6386 mean_circ: 0.948949
Ran for 148550 frames Cell Events: 6414 mean_circ: 0.948931
Ran for 149050 frames Cell Events: 6442 mean_circ: 0.94892
Ran for 149550 frames Cell Events: 6456 mean_circ: 0.948941
Ran for 150050 frames Cell Events: 6482 mean_circ: 0.948953
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Ran for 152550 frames Cell Events: 6590 mean_circ: 0.949003
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Ran for 177050 frames Cell Events: 7719 mean_circ: 0.948902
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Ran for 196550 frames Cell Events: 8609 mean_circ: 0.948693
Ran for 197050 frames Cell Events: 8631 mean_circ: 0.948715
Ran for 197550 frames Cell Events: 8650 mean_circ: 0.948723
Ran for 200050 frames Cell Events: 8744 mean_circ: 0.948715
Ran for 200550 frames Cell Events: 8776 mean_circ: 0.94868
Ran for 201050 frames Cell Events: 8791 mean_circ: 0.948685
Ran for 202050 frames Cell Events: 8860 mean_circ: 0.948696
Ran for 203050 frames Cell Events: 8904 mean_circ: 0.948659
End this measurment..
Stopping time: 6.28004e+008
****************************************
Measurement finished
Mean Circularitiy: 0.948666
Mean Area: 581.806
Mean FL-1 intensity: 3286.65
Mean FL-2 intensity: 4600.25
Mean FL-3 intensity: 0
frames processed: 200962
Total Time: 101650ms
Number of Runs (new bg img) 0
Frames collected: 203160
Skipped frames: 29257
Rescued Frames: 1518942
Samples DAQ: 50789936
Samples written: 101579532
Samples per Channel: 50789766
FPS: 1998.61
Samples per second: 499651
Expected samples/s: 500000
Events: 8907
Events per second: 87.6238
jumped back (PMT): 4981
#Frames read, frequency
# 0 frames :286201
# 1 frames :104497
# 2 frames :4556
# 3 frames :1176
# 4 frames :634
# 5 frames :198
# 6 frames :2958
# 7 frames :1860
# 8 frames :1320
# 9 frames :573
# 10 frames :436
# 11 frames :262
# 12 frames :325
# 13 frames :212
# 14 frames :262
# 15 frames :196
# 16 frames :108
# 17 frames :92
# 18 frames :52
# 19 frames :47
# 20 frames :62
# 21 frames :49
# 22 frames :33
# 23 frames :26
# 24 frames :24
# 25 frames :22
# 26 frames :16
# 27 frames :15
# 28 frames :8
# 29 frames :12
# 30 frames :5
# 31 frames :12
# 32 frames :4
# 33 frames :5
# 34 frames :1
# 35 frames :3
# 36 frames :5
# 37 frames :2
# 38 frames :1
# 39 frames :1
# 40 frames :2
# 41 frames :3
# 43 frames :4
# 44 frames :3
# 46 frames :4
# 49 frames :1
# 50 frames :1
# 54 frames :1
# 64 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.3277
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0