Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_12.48.00
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "1f078f236ecb38f5524dcd4d1f5231d0",
"name": "M1_20170604_1739_dsRNA_E2_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:48:21"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8307 (max 8308) in '8307'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.2476
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.76141e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 8 mean_circ: 0.956218
Ran for 1550 frames Cell Events: 46 mean_circ: 0.94624
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Ran for 3550 frames Cell Events: 98 mean_circ: 0.947386
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Ran for 5550 frames Cell Events: 164 mean_circ: 0.944534
Ran for 6550 frames Cell Events: 198 mean_circ: 0.944729
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Ran for 87050 frames Cell Events: 2575 mean_circ: 0.94824
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Ran for 91050 frames Cell Events: 2696 mean_circ: 0.948329
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Ran for 96550 frames Cell Events: 2858 mean_circ: 0.948491
Ran for 97550 frames Cell Events: 2888 mean_circ: 0.948499
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Ran for 121550 frames Cell Events: 3619 mean_circ: 0.948533
Ran for 122050 frames Cell Events: 3631 mean_circ: 0.94854
Ran for 123050 frames Cell Events: 3665 mean_circ: 0.948559
Ran for 124050 frames Cell Events: 3698 mean_circ: 0.948587
Ran for 124550 frames Cell Events: 3711 mean_circ: 0.948608
Ran for 125550 frames Cell Events: 3740 mean_circ: 0.948689
Ran for 126050 frames Cell Events: 3757 mean_circ: 0.948609
Ran for 126550 frames Cell Events: 3768 mean_circ: 0.948601
Ran for 127550 frames Cell Events: 3805 mean_circ: 0.948652
Ran for 128050 frames Cell Events: 3812 mean_circ: 0.948661
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Ran for 131050 frames Cell Events: 3903 mean_circ: 0.948604
Ran for 131550 frames Cell Events: 3908 mean_circ: 0.948619
Ran for 132050 frames Cell Events: 3917 mean_circ: 0.948633
Ran for 132550 frames Cell Events: 3931 mean_circ: 0.948632
Ran for 133050 frames Cell Events: 3950 mean_circ: 0.948575
Ran for 133550 frames Cell Events: 3964 mean_circ: 0.948553
Ran for 134050 frames Cell Events: 3978 mean_circ: 0.948532
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Ran for 193050 frames Cell Events: 5808 mean_circ: 0.949096
Ran for 194050 frames Cell Events: 5836 mean_circ: 0.949031
Ran for 194550 frames Cell Events: 5858 mean_circ: 0.949045
Ran for 195050 frames Cell Events: 5866 mean_circ: 0.949004
Ran for 196550 frames Cell Events: 5915 mean_circ: 0.949008
Ran for 197550 frames Cell Events: 5940 mean_circ: 0.949042
Ran for 198050 frames Cell Events: 5955 mean_circ: 0.949036
Ran for 198550 frames Cell Events: 5967 mean_circ: 0.949047
Ran for 199050 frames Cell Events: 5982 mean_circ: 0.949037
Ran for 199550 frames Cell Events: 5992 mean_circ: 0.949067
Ran for 200050 frames Cell Events: 6002 mean_circ: 0.949068
Ran for 201050 frames Cell Events: 6038 mean_circ: 0.949009
Ran for 202050 frames Cell Events: 6065 mean_circ: 0.949033
Ran for 202550 frames Cell Events: 6074 mean_circ: 0.949032
Ran for 203550 frames Cell Events: 6097 mean_circ: 0.949017
Ran for 204050 frames Cell Events: 6116 mean_circ: 0.949056
Ran for 205550 frames Cell Events: 6167 mean_circ: 0.949053
Ran for 206550 frames Cell Events: 6198 mean_circ: 0.949003
Ran for 207050 frames Cell Events: 6213 mean_circ: 0.949016
Ran for 208050 frames Cell Events: 6244 mean_circ: 0.949016
Ran for 208550 frames Cell Events: 6262 mean_circ: 0.949008
Ran for 210550 frames Cell Events: 6307 mean_circ: 0.948947
Ran for 211550 frames Cell Events: 6346 mean_circ: 0.948915
Ran for 212050 frames Cell Events: 6361 mean_circ: 0.948927
Ran for 212550 frames Cell Events: 6383 mean_circ: 0.948958
Ran for 213550 frames Cell Events: 6438 mean_circ: 0.948993
Ran for 214550 frames Cell Events: 6456 mean_circ: 0.948956
Ran for 215550 frames Cell Events: 6493 mean_circ: 0.948894
Ran for 216050 frames Cell Events: 6508 mean_circ: 0.948865
Ran for 216550 frames Cell Events: 6517 mean_circ: 0.948871
Ran for 218050 frames Cell Events: 6577 mean_circ: 0.948865
Ran for 218550 frames Cell Events: 6596 mean_circ: 0.948873
Ran for 219050 frames Cell Events: 6612 mean_circ: 0.948853
Ran for 219550 frames Cell Events: 6629 mean_circ: 0.948845
Ran for 220050 frames Cell Events: 6641 mean_circ: 0.948883
Ran for 220550 frames Cell Events: 6657 mean_circ: 0.948883
Ran for 221050 frames Cell Events: 6681 mean_circ: 0.948893
Ran for 221550 frames Cell Events: 6693 mean_circ: 0.948883
Ran for 222050 frames Cell Events: 6711 mean_circ: 0.948908
Ran for 222550 frames Cell Events: 6734 mean_circ: 0.948893
Ran for 223050 frames Cell Events: 6750 mean_circ: 0.948876
Ran for 224050 frames Cell Events: 6777 mean_circ: 0.948892
Ran for 225550 frames Cell Events: 6829 mean_circ: 0.948834
Ran for 226050 frames Cell Events: 6845 mean_circ: 0.948802
Ran for 226550 frames Cell Events: 6867 mean_circ: 0.948846
Ran for 227550 frames Cell Events: 6909 mean_circ: 0.94885
Ran for 228050 frames Cell Events: 6929 mean_circ: 0.948849
Ran for 229550 frames Cell Events: 6975 mean_circ: 0.948819
Ran for 230050 frames Cell Events: 6993 mean_circ: 0.948814
Ran for 230550 frames Cell Events: 7005 mean_circ: 0.948813
Ran for 232050 frames Cell Events: 7057 mean_circ: 0.948848
Ran for 232550 frames Cell Events: 7077 mean_circ: 0.948869
Ran for 234550 frames Cell Events: 7176 mean_circ: 0.948837
Ran for 235050 frames Cell Events: 7194 mean_circ: 0.948884
Ran for 235550 frames Cell Events: 7210 mean_circ: 0.948848
Ran for 236550 frames Cell Events: 7237 mean_circ: 0.948849
Ran for 237050 frames Cell Events: 7251 mean_circ: 0.948871
Ran for 237550 frames Cell Events: 7269 mean_circ: 0.948895
Ran for 238550 frames Cell Events: 7294 mean_circ: 0.948879
Ran for 239050 frames Cell Events: 7309 mean_circ: 0.948882
Ran for 240050 frames Cell Events: 7350 mean_circ: 0.948911
Ran for 240550 frames Cell Events: 7367 mean_circ: 0.948948
Ran for 241550 frames Cell Events: 7390 mean_circ: 0.948907
Ran for 242550 frames Cell Events: 7423 mean_circ: 0.948878
Ran for 243050 frames Cell Events: 7442 mean_circ: 0.94887
Ran for 243550 frames Cell Events: 7452 mean_circ: 0.948886
Ran for 244050 frames Cell Events: 7463 mean_circ: 0.948885
Ran for 245050 frames Cell Events: 7496 mean_circ: 0.948861
Ran for 245550 frames Cell Events: 7507 mean_circ: 0.948858
Ran for 246050 frames Cell Events: 7517 mean_circ: 0.948875
Ran for 246550 frames Cell Events: 7527 mean_circ: 0.94889
Ran for 247550 frames Cell Events: 7558 mean_circ: 0.948878
Ran for 248050 frames Cell Events: 7578 mean_circ: 0.948869
Ran for 248550 frames Cell Events: 7589 mean_circ: 0.948853
Ran for 249050 frames Cell Events: 7601 mean_circ: 0.948869
Ran for 249550 frames Cell Events: 7620 mean_circ: 0.948896
Ran for 252050 frames Cell Events: 7712 mean_circ: 0.948984
Ran for 254550 frames Cell Events: 7811 mean_circ: 0.948999
Ran for 255050 frames Cell Events: 7829 mean_circ: 0.949011
Ran for 255550 frames Cell Events: 7844 mean_circ: 0.949011
Ran for 256050 frames Cell Events: 7859 mean_circ: 0.948989
Ran for 256550 frames Cell Events: 7880 mean_circ: 0.948992
Ran for 258050 frames Cell Events: 7930 mean_circ: 0.948997
Ran for 258550 frames Cell Events: 7943 mean_circ: 0.948985
Ran for 259050 frames Cell Events: 7960 mean_circ: 0.948986
Ran for 259550 frames Cell Events: 7987 mean_circ: 0.948986
Ran for 260050 frames Cell Events: 8001 mean_circ: 0.949003
Ran for 261550 frames Cell Events: 8041 mean_circ: 0.949041
Ran for 263050 frames Cell Events: 8100 mean_circ: 0.948999
Ran for 263550 frames Cell Events: 8112 mean_circ: 0.949006
Ran for 264050 frames Cell Events: 8123 mean_circ: 0.948989
Ran for 264550 frames Cell Events: 8133 mean_circ: 0.949005
Ran for 265050 frames Cell Events: 8141 mean_circ: 0.949002
Ran for 267050 frames Cell Events: 8187 mean_circ: 0.948972
Ran for 267550 frames Cell Events: 8202 mean_circ: 0.948943
Ran for 268050 frames Cell Events: 8225 mean_circ: 0.948936
Ran for 269050 frames Cell Events: 8243 mean_circ: 0.948949
End this measurment..
Stopping time: 1.76154e+009
****************************************
Measurement finished
Mean Circularitiy: 0.94901
Mean Area: 612.344
Mean FL-1 intensity: 3520.33
Mean FL-2 intensity: 4249.98
Mean FL-3 intensity: 0
frames processed: 267688
Total Time: 135414ms
Number of Runs (new bg img) 0
Frames collected: 270629
Skipped frames: 102926
Rescued Frames: 3598389
Samples DAQ: 67658885
Samples written: 135314266
Samples per Channel: 67657133
FPS: 1998.54
Samples per second: 499633
Expected samples/s: 500000
Events: 8308
Events per second: 61.3528
jumped back (PMT): 4964
#Frames read, frequency
# 0 frames :383338
# 1 frames :138931
# 2 frames :5136
# 3 frames :2028
# 4 frames :1164
# 5 frames :647
# 6 frames :1345
# 7 frames :1925
# 8 frames :1634
# 9 frames :1050
# 10 frames :703
# 11 frames :524
# 12 frames :429
# 13 frames :360
# 14 frames :283
# 15 frames :247
# 16 frames :228
# 17 frames :211
# 18 frames :168
# 19 frames :132
# 20 frames :109
# 21 frames :86
# 22 frames :57
# 23 frames :60
# 24 frames :58
# 25 frames :39
# 26 frames :29
# 27 frames :24
# 28 frames :18
# 29 frames :23
# 30 frames :20
# 31 frames :25
# 32 frames :14
# 33 frames :18
# 34 frames :11
# 35 frames :6
# 36 frames :9
# 37 frames :1
# 38 frames :10
# 39 frames :5
# 40 frames :8
# 41 frames :6
# 42 frames :4
# 43 frames :5
# 44 frames :3
# 45 frames :2
# 46 frames :2
# 47 frames :5
# 48 frames :3
# 49 frames :2
# 50 frames :3
# 52 frames :2
# 53 frames :2
# 54 frames :1
# 57 frames :1
# 58 frames :2
# 59 frames :1
# 60 frames :1
# 65 frames :1
# 68 frames :1
# 74 frames :1
# 75 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.2476
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0