Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_19.51.52
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "0f6c5f98929908c07363c73959592714",
"name": "M1_20170523_1449_dsRNA_G3_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:52:18"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9814 (max 9816) in '9814'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0235
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 7.14378e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 13 mean_circ: 0.844831
Ran for 1050 frames Cell Events: 31 mean_circ: 0.918093
Ran for 2050 frames Cell Events: 67 mean_circ: 0.932895
Ran for 2550 frames Cell Events: 85 mean_circ: 0.937596
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Ran for 3550 frames Cell Events: 123 mean_circ: 0.941099
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Ran for 6050 frames Cell Events: 174 mean_circ: 0.942317
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Ran for 83050 frames Cell Events: 3444 mean_circ: 0.948072
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Ran for 87050 frames Cell Events: 3612 mean_circ: 0.948109
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Ran for 91050 frames Cell Events: 3760 mean_circ: 0.947969
Ran for 92550 frames Cell Events: 3857 mean_circ: 0.948
Ran for 93050 frames Cell Events: 3874 mean_circ: 0.948044
Ran for 93550 frames Cell Events: 3909 mean_circ: 0.948104
Ran for 94550 frames Cell Events: 3964 mean_circ: 0.948153
Ran for 95550 frames Cell Events: 4004 mean_circ: 0.948232
Ran for 96050 frames Cell Events: 4018 mean_circ: 0.948244
Ran for 96550 frames Cell Events: 4052 mean_circ: 0.948282
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Ran for 100550 frames Cell Events: 4224 mean_circ: 0.948514
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Ran for 102550 frames Cell Events: 4310 mean_circ: 0.948435
Ran for 103050 frames Cell Events: 4334 mean_circ: 0.948427
Ran for 103550 frames Cell Events: 4363 mean_circ: 0.948409
Ran for 104050 frames Cell Events: 4381 mean_circ: 0.94843
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Ran for 123050 frames Cell Events: 5314 mean_circ: 0.947833
Ran for 123550 frames Cell Events: 5334 mean_circ: 0.947847
Ran for 124050 frames Cell Events: 5357 mean_circ: 0.947854
Ran for 125050 frames Cell Events: 5409 mean_circ: 0.947902
Ran for 125550 frames Cell Events: 5428 mean_circ: 0.947914
Ran for 127550 frames Cell Events: 5529 mean_circ: 0.947846
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Ran for 132050 frames Cell Events: 5779 mean_circ: 0.947834
Ran for 132550 frames Cell Events: 5800 mean_circ: 0.947832
Ran for 133050 frames Cell Events: 5822 mean_circ: 0.94789
Ran for 134050 frames Cell Events: 5863 mean_circ: 0.947913
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Ran for 142550 frames Cell Events: 6269 mean_circ: 0.947914
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Ran for 145050 frames Cell Events: 6391 mean_circ: 0.947836
Ran for 145550 frames Cell Events: 6417 mean_circ: 0.94785
Ran for 146050 frames Cell Events: 6448 mean_circ: 0.947898
Ran for 147050 frames Cell Events: 6496 mean_circ: 0.947854
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Ran for 151050 frames Cell Events: 6695 mean_circ: 0.947833
Ran for 152550 frames Cell Events: 6761 mean_circ: 0.947854
Ran for 153550 frames Cell Events: 6804 mean_circ: 0.947785
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Ran for 157050 frames Cell Events: 6970 mean_circ: 0.947723
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Ran for 208050 frames Cell Events: 9554 mean_circ: 0.947803
Ran for 208550 frames Cell Events: 9573 mean_circ: 0.947807
Ran for 209050 frames Cell Events: 9611 mean_circ: 0.947759
Ran for 210050 frames Cell Events: 9640 mean_circ: 0.947728
Ran for 210550 frames Cell Events: 9663 mean_circ: 0.947732
Ran for 211050 frames Cell Events: 9689 mean_circ: 0.947745
Ran for 212050 frames Cell Events: 9759 mean_circ: 0.9477
Ran for 212550 frames Cell Events: 9785 mean_circ: 0.947724
End this measurment..
Stopping time: 7.14484e+008
****************************************
Measurement finished
Mean Circularitiy: 0.947756
Mean Area: 575.764
Mean FL-1 intensity: 3456.16
Mean FL-2 intensity: 4149.54
Mean FL-3 intensity: 0
frames processed: 211553
Total Time: 106593ms
Number of Runs (new bg img) 0
Frames collected: 213034
Skipped frames: 31399
Rescued Frames: 1300125
Samples DAQ: 53259147
Samples written: 106518078
Samples per Channel: 53259039
FPS: 1998.57
Samples per second: 499647
Expected samples/s: 500000
Events: 9816
Events per second: 92.0883
jumped back (PMT): 5136
#Frames read, frequency
# 0 frames :305988
# 1 frames :103263
# 2 frames :4893
# 3 frames :1227
# 4 frames :726
# 5 frames :302
# 6 frames :3110
# 7 frames :1644
# 8 frames :1371
# 9 frames :665
# 10 frames :471
# 11 frames :307
# 12 frames :351
# 13 frames :217
# 14 frames :295
# 15 frames :213
# 16 frames :176
# 17 frames :102
# 18 frames :96
# 19 frames :61
# 20 frames :69
# 21 frames :69
# 22 frames :56
# 23 frames :38
# 24 frames :35
# 25 frames :31
# 26 frames :28
# 27 frames :22
# 28 frames :22
# 29 frames :20
# 30 frames :18
# 31 frames :14
# 32 frames :14
# 33 frames :7
# 34 frames :6
# 35 frames :10
# 36 frames :6
# 37 frames :8
# 38 frames :4
# 39 frames :6
# 40 frames :7
# 41 frames :3
# 42 frames :1
# 43 frames :4
# 44 frames :2
# 45 frames :1
# 47 frames :4
# 48 frames :2
# 49 frames :1
# 50 frames :3
# 52 frames :1
# 54 frames :3
# 55 frames :1
# 56 frames :1
# 63 frames :1
# 68 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0235
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0