Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_13.00.54
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "e44d632a26407bdd1697cb50edb914b8",
"name": "M1_20170604_2030_dsRNA_G7_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "11:01:15"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8259 (max 8260) in '8259'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1976
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.77165e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 20 mean_circ: 0.9556
Ran for 3550 frames Cell Events: 159 mean_circ: 0.945901
Ran for 4050 frames Cell Events: 170 mean_circ: 0.946557
Ran for 4550 frames Cell Events: 184 mean_circ: 0.943973
Ran for 5050 frames Cell Events: 202 mean_circ: 0.943798
Ran for 5550 frames Cell Events: 222 mean_circ: 0.944556
Ran for 8550 frames Cell Events: 326 mean_circ: 0.945465
Ran for 9050 frames Cell Events: 348 mean_circ: 0.945674
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Ran for 10050 frames Cell Events: 381 mean_circ: 0.946471
Ran for 10550 frames Cell Events: 394 mean_circ: 0.946803
Ran for 12050 frames Cell Events: 444 mean_circ: 0.947198
Ran for 12550 frames Cell Events: 465 mean_circ: 0.946768
Ran for 15050 frames Cell Events: 537 mean_circ: 0.946648
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Ran for 16550 frames Cell Events: 590 mean_circ: 0.946096
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Ran for 72550 frames Cell Events: 2778 mean_circ: 0.945239
Ran for 73550 frames Cell Events: 2832 mean_circ: 0.945371
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Ran for 78050 frames Cell Events: 3000 mean_circ: 0.945644
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Ran for 83050 frames Cell Events: 3229 mean_circ: 0.945536
Ran for 83550 frames Cell Events: 3247 mean_circ: 0.945536
Ran for 84050 frames Cell Events: 3269 mean_circ: 0.945554
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Ran for 86550 frames Cell Events: 3380 mean_circ: 0.945837
Ran for 87050 frames Cell Events: 3396 mean_circ: 0.945825
Ran for 88050 frames Cell Events: 3438 mean_circ: 0.945631
Ran for 88550 frames Cell Events: 3461 mean_circ: 0.945569
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Ran for 91550 frames Cell Events: 3590 mean_circ: 0.945419
Ran for 92050 frames Cell Events: 3605 mean_circ: 0.945449
Ran for 93050 frames Cell Events: 3645 mean_circ: 0.945437
Ran for 94050 frames Cell Events: 3698 mean_circ: 0.945394
Ran for 94550 frames Cell Events: 3723 mean_circ: 0.945399
Ran for 95050 frames Cell Events: 3745 mean_circ: 0.945412
Ran for 96050 frames Cell Events: 3796 mean_circ: 0.945374
Ran for 96550 frames Cell Events: 3811 mean_circ: 0.945404
Ran for 97550 frames Cell Events: 3844 mean_circ: 0.945282
Ran for 98050 frames Cell Events: 3857 mean_circ: 0.94527
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Ran for 101050 frames Cell Events: 3960 mean_circ: 0.945342
Ran for 101550 frames Cell Events: 3974 mean_circ: 0.945357
Ran for 102050 frames Cell Events: 3987 mean_circ: 0.94538
Ran for 102550 frames Cell Events: 3998 mean_circ: 0.94531
Ran for 103050 frames Cell Events: 4011 mean_circ: 0.945348
Ran for 104050 frames Cell Events: 4064 mean_circ: 0.945323
Ran for 104550 frames Cell Events: 4080 mean_circ: 0.945329
Ran for 105050 frames Cell Events: 4098 mean_circ: 0.945315
Ran for 105550 frames Cell Events: 4118 mean_circ: 0.945321
Ran for 106550 frames Cell Events: 4163 mean_circ: 0.945386
Ran for 107050 frames Cell Events: 4177 mean_circ: 0.945388
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Ran for 123050 frames Cell Events: 4869 mean_circ: 0.945475
Ran for 123550 frames Cell Events: 4885 mean_circ: 0.945475
Ran for 124550 frames Cell Events: 4938 mean_circ: 0.945527
Ran for 125050 frames Cell Events: 4956 mean_circ: 0.945508
Ran for 126050 frames Cell Events: 4998 mean_circ: 0.945549
Ran for 126550 frames Cell Events: 5023 mean_circ: 0.945541
Ran for 127550 frames Cell Events: 5076 mean_circ: 0.945583
Ran for 128050 frames Cell Events: 5097 mean_circ: 0.945619
Ran for 128550 frames Cell Events: 5120 mean_circ: 0.94567
Ran for 129050 frames Cell Events: 5142 mean_circ: 0.945706
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Ran for 132050 frames Cell Events: 5267 mean_circ: 0.945731
Ran for 132550 frames Cell Events: 5290 mean_circ: 0.945744
Ran for 133050 frames Cell Events: 5313 mean_circ: 0.945743
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Ran for 134050 frames Cell Events: 5362 mean_circ: 0.945766
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Ran for 141050 frames Cell Events: 5655 mean_circ: 0.945909
Ran for 141550 frames Cell Events: 5684 mean_circ: 0.945861
Ran for 142550 frames Cell Events: 5719 mean_circ: 0.945858
Ran for 143050 frames Cell Events: 5741 mean_circ: 0.945831
Ran for 144050 frames Cell Events: 5784 mean_circ: 0.945868
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Ran for 145550 frames Cell Events: 5836 mean_circ: 0.945907
Ran for 146050 frames Cell Events: 5855 mean_circ: 0.9459
Ran for 146550 frames Cell Events: 5876 mean_circ: 0.945931
Ran for 147050 frames Cell Events: 5894 mean_circ: 0.945944
Ran for 147550 frames Cell Events: 5907 mean_circ: 0.945942
Ran for 148050 frames Cell Events: 5927 mean_circ: 0.945943
Ran for 149050 frames Cell Events: 5960 mean_circ: 0.945964
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Ran for 172050 frames Cell Events: 6874 mean_circ: 0.946195
Ran for 174050 frames Cell Events: 6958 mean_circ: 0.946204
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Ran for 197550 frames Cell Events: 8024 mean_circ: 0.946036
Ran for 198050 frames Cell Events: 8055 mean_circ: 0.946015
Ran for 198550 frames Cell Events: 8074 mean_circ: 0.946019
Ran for 199050 frames Cell Events: 8089 mean_circ: 0.946042
Ran for 199550 frames Cell Events: 8103 mean_circ: 0.946062
Ran for 200550 frames Cell Events: 8125 mean_circ: 0.946084
Ran for 201050 frames Cell Events: 8141 mean_circ: 0.94606
Ran for 201550 frames Cell Events: 8160 mean_circ: 0.946039
Ran for 203050 frames Cell Events: 8224 mean_circ: 0.946011
Ran for 203550 frames Cell Events: 8239 mean_circ: 0.946012
End this measurment..
Stopping time: 1.77175e+009
****************************************
Measurement finished
Mean Circularitiy: 0.946023
Mean Area: 619.963
Mean FL-1 intensity: 3542.03
Mean FL-2 intensity: 4381.97
Mean FL-3 intensity: 0
frames processed: 202735
Total Time: 102083ms
Number of Runs (new bg img) 0
Frames collected: 204010
Skipped frames: 176791
Rescued Frames: 6325800
Samples DAQ: 51005244
Samples written: 102005056
Samples per Channel: 51002528
FPS: 1998.48
Samples per second: 499620
Expected samples/s: 500000
Events: 8260
Events per second: 80.9148
jumped back (PMT): 5402
#Frames read, frequency
# 0 frames :283463
# 1 frames :108645
# 2 frames :4811
# 3 frames :1408
# 4 frames :648
# 5 frames :251
# 6 frames :2967
# 7 frames :1692
# 8 frames :1319
# 9 frames :604
# 10 frames :448
# 11 frames :260
# 12 frames :334
# 13 frames :161
# 14 frames :230
# 15 frames :148
# 16 frames :138
# 17 frames :94
# 18 frames :54
# 19 frames :35
# 20 frames :42
# 21 frames :34
# 22 frames :32
# 23 frames :28
# 24 frames :22
# 25 frames :19
# 26 frames :12
# 27 frames :13
# 28 frames :6
# 29 frames :10
# 30 frames :9
# 31 frames :4
# 32 frames :6
# 33 frames :3
# 34 frames :4
# 35 frames :1
# 36 frames :2
# 37 frames :1
# 38 frames :3
# 40 frames :3
# 41 frames :2
# 43 frames :1
# 44 frames :1
# 45 frames :1
# 56 frames :1
# 58 frames :1
# 60 frames :1
# 72 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1976
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0