Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_12.59.07
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "0540752715b47e3f9de38b799eb7cfb1",
"name": "M1_20170604_2007_dsRNA_H2_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:59:26"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8038 (max 8039) in '8038'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.2404
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.77026e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 2 mean_circ: 0.937294
Ran for 1550 frames Cell Events: 39 mean_circ: 0.952049
Ran for 2550 frames Cell Events: 64 mean_circ: 0.950273
Ran for 3050 frames Cell Events: 76 mean_circ: 0.951615
Ran for 3550 frames Cell Events: 93 mean_circ: 0.951056
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Ran for 4550 frames Cell Events: 124 mean_circ: 0.947471
Ran for 6050 frames Cell Events: 180 mean_circ: 0.949686
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Ran for 8550 frames Cell Events: 236 mean_circ: 0.950803
Ran for 9050 frames Cell Events: 255 mean_circ: 0.950333
Ran for 9550 frames Cell Events: 268 mean_circ: 0.950671
Ran for 10050 frames Cell Events: 279 mean_circ: 0.951113
Ran for 11050 frames Cell Events: 316 mean_circ: 0.949816
Ran for 11550 frames Cell Events: 325 mean_circ: 0.950027
Ran for 12050 frames Cell Events: 351 mean_circ: 0.950741
Ran for 12550 frames Cell Events: 372 mean_circ: 0.950663
Ran for 13050 frames Cell Events: 380 mean_circ: 0.950958
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Ran for 14050 frames Cell Events: 417 mean_circ: 0.9496
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Ran for 73550 frames Cell Events: 2504 mean_circ: 0.948962
Ran for 74050 frames Cell Events: 2519 mean_circ: 0.949066
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Ran for 83550 frames Cell Events: 2876 mean_circ: 0.949079
Ran for 84050 frames Cell Events: 2893 mean_circ: 0.949063
Ran for 84550 frames Cell Events: 2906 mean_circ: 0.949085
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Ran for 85550 frames Cell Events: 2942 mean_circ: 0.948962
Ran for 86050 frames Cell Events: 2964 mean_circ: 0.949007
Ran for 86550 frames Cell Events: 2978 mean_circ: 0.949032
Ran for 87550 frames Cell Events: 3007 mean_circ: 0.949016
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Ran for 93050 frames Cell Events: 3251 mean_circ: 0.948766
Ran for 93550 frames Cell Events: 3281 mean_circ: 0.948686
Ran for 94050 frames Cell Events: 3299 mean_circ: 0.948685
Ran for 95050 frames Cell Events: 3358 mean_circ: 0.948567
Ran for 96550 frames Cell Events: 3422 mean_circ: 0.948481
Ran for 97050 frames Cell Events: 3436 mean_circ: 0.948512
Ran for 97550 frames Cell Events: 3455 mean_circ: 0.948436
Ran for 98050 frames Cell Events: 3472 mean_circ: 0.948464
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Ran for 100050 frames Cell Events: 3553 mean_circ: 0.948238
Ran for 101050 frames Cell Events: 3586 mean_circ: 0.948212
Ran for 102550 frames Cell Events: 3640 mean_circ: 0.948265
Ran for 103050 frames Cell Events: 3665 mean_circ: 0.948142
Ran for 104050 frames Cell Events: 3695 mean_circ: 0.948109
Ran for 107550 frames Cell Events: 3837 mean_circ: 0.947895
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Ran for 121550 frames Cell Events: 4410 mean_circ: 0.948139
Ran for 122050 frames Cell Events: 4431 mean_circ: 0.94807
Ran for 122550 frames Cell Events: 4448 mean_circ: 0.948083
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Ran for 125550 frames Cell Events: 4562 mean_circ: 0.948096
Ran for 126550 frames Cell Events: 4613 mean_circ: 0.948133
Ran for 127050 frames Cell Events: 4626 mean_circ: 0.948155
Ran for 127550 frames Cell Events: 4652 mean_circ: 0.948189
Ran for 128050 frames Cell Events: 4669 mean_circ: 0.948168
Ran for 129050 frames Cell Events: 4699 mean_circ: 0.948154
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Ran for 130050 frames Cell Events: 4734 mean_circ: 0.94818
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Ran for 132050 frames Cell Events: 4811 mean_circ: 0.948095
Ran for 132550 frames Cell Events: 4831 mean_circ: 0.948132
Ran for 133050 frames Cell Events: 4851 mean_circ: 0.948132
Ran for 133550 frames Cell Events: 4864 mean_circ: 0.948073
Ran for 134050 frames Cell Events: 4885 mean_circ: 0.948118
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Ran for 136050 frames Cell Events: 4954 mean_circ: 0.948152
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Ran for 138050 frames Cell Events: 5047 mean_circ: 0.948265
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Ran for 141050 frames Cell Events: 5158 mean_circ: 0.948242
Ran for 141550 frames Cell Events: 5175 mean_circ: 0.948127
Ran for 142550 frames Cell Events: 5211 mean_circ: 0.948168
Ran for 143550 frames Cell Events: 5243 mean_circ: 0.948179
Ran for 145050 frames Cell Events: 5302 mean_circ: 0.94822
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Ran for 146050 frames Cell Events: 5338 mean_circ: 0.948206
Ran for 146550 frames Cell Events: 5359 mean_circ: 0.948234
Ran for 147550 frames Cell Events: 5396 mean_circ: 0.948203
Ran for 148050 frames Cell Events: 5408 mean_circ: 0.948223
Ran for 148550 frames Cell Events: 5429 mean_circ: 0.948116
Ran for 149050 frames Cell Events: 5448 mean_circ: 0.948099
Ran for 150050 frames Cell Events: 5485 mean_circ: 0.948038
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Ran for 151050 frames Cell Events: 5519 mean_circ: 0.948038
Ran for 151550 frames Cell Events: 5531 mean_circ: 0.948049
Ran for 152050 frames Cell Events: 5543 mean_circ: 0.948015
Ran for 153050 frames Cell Events: 5594 mean_circ: 0.948088
Ran for 153550 frames Cell Events: 5612 mean_circ: 0.948121
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Ran for 201550 frames Cell Events: 7395 mean_circ: 0.948359
Ran for 202050 frames Cell Events: 7408 mean_circ: 0.948348
Ran for 202550 frames Cell Events: 7424 mean_circ: 0.948315
Ran for 203050 frames Cell Events: 7448 mean_circ: 0.948326
Ran for 203550 frames Cell Events: 7466 mean_circ: 0.948295
Ran for 204050 frames Cell Events: 7484 mean_circ: 0.9483
Ran for 204550 frames Cell Events: 7496 mean_circ: 0.94832
Ran for 205050 frames Cell Events: 7513 mean_circ: 0.948302
Ran for 206050 frames Cell Events: 7550 mean_circ: 0.948306
Ran for 209050 frames Cell Events: 7697 mean_circ: 0.948221
Ran for 209550 frames Cell Events: 7719 mean_circ: 0.948218
Ran for 211550 frames Cell Events: 7802 mean_circ: 0.94826
Ran for 212050 frames Cell Events: 7825 mean_circ: 0.948277
Ran for 212550 frames Cell Events: 7847 mean_circ: 0.94825
Ran for 213550 frames Cell Events: 7896 mean_circ: 0.948192
Ran for 214050 frames Cell Events: 7915 mean_circ: 0.948137
Ran for 215050 frames Cell Events: 7960 mean_circ: 0.948122
Ran for 215550 frames Cell Events: 7977 mean_circ: 0.948141
Ran for 216050 frames Cell Events: 8003 mean_circ: 0.948157
Ran for 216550 frames Cell Events: 8033 mean_circ: 0.948157
End this measurment..
Stopping time: 1.77037e+009
****************************************
Measurement finished
Mean Circularitiy: 0.948057
Mean Area: 611.961
Mean FL-1 intensity: 3406.47
Mean FL-2 intensity: 4016.52
Mean FL-3 intensity: 0
frames processed: 215535
Total Time: 108501ms
Number of Runs (new bg img) 0
Frames collected: 216889
Skipped frames: 164106
Rescued Frames: 5963390
Samples DAQ: 54222272
Samples written: 108444220
Samples per Channel: 54222110
FPS: 1998.95
Samples per second: 499737
Expected samples/s: 500000
Events: 8040
Events per second: 74.1005
jumped back (PMT): 4433
#Frames read, frequency
# 0 frames :313661
# 1 frames :103901
# 2 frames :4326
# 3 frames :1611
# 4 frames :960
# 5 frames :510
# 6 frames :1265
# 7 frames :1905
# 8 frames :1285
# 9 frames :820
# 10 frames :543
# 11 frames :468
# 12 frames :456
# 13 frames :346
# 14 frames :305
# 15 frames :237
# 16 frames :194
# 17 frames :166
# 18 frames :115
# 19 frames :96
# 20 frames :79
# 21 frames :72
# 22 frames :69
# 23 frames :50
# 24 frames :44
# 25 frames :34
# 26 frames :38
# 27 frames :23
# 28 frames :29
# 29 frames :25
# 30 frames :18
# 31 frames :20
# 32 frames :16
# 33 frames :5
# 34 frames :4
# 35 frames :6
# 36 frames :2
# 37 frames :6
# 38 frames :3
# 39 frames :9
# 40 frames :4
# 41 frames :4
# 42 frames :2
# 43 frames :5
# 44 frames :1
# 45 frames :2
# 46 frames :4
# 47 frames :4
# 48 frames :1
# 50 frames :1
# 52 frames :1
# 53 frames :1
# 59 frames :1
# 60 frames :2
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.2404
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0