Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_17.58.35
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "8da2377d4046b7928f7eb717e8f789e9",
"name": "M1_20170518_1703_dsRNA_I2_repeat.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "15:58:57"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 7988 (max 7989) in '7988'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 27.379
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.90459e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 18 mean_circ: 0.941178
Ran for 1050 frames Cell Events: 30 mean_circ: 0.936026
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Ran for 79050 frames Cell Events: 1967 mean_circ: 0.952294
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Ran for 121550 frames Cell Events: 3469 mean_circ: 0.952566
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Ran for 124050 frames Cell Events: 3565 mean_circ: 0.952621
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Ran for 127050 frames Cell Events: 3686 mean_circ: 0.952504
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Ran for 184050 frames Cell Events: 6023 mean_circ: 0.952966
Ran for 184550 frames Cell Events: 6041 mean_circ: 0.95298
Ran for 185050 frames Cell Events: 6065 mean_circ: 0.952977
Ran for 185550 frames Cell Events: 6098 mean_circ: 0.952997
Ran for 186050 frames Cell Events: 6119 mean_circ: 0.952928
Ran for 186550 frames Cell Events: 6141 mean_circ: 0.952929
Ran for 188550 frames Cell Events: 6254 mean_circ: 0.952927
Ran for 189550 frames Cell Events: 6321 mean_circ: 0.952979
Ran for 190550 frames Cell Events: 6374 mean_circ: 0.953038
Ran for 191050 frames Cell Events: 6392 mean_circ: 0.952973
Ran for 192050 frames Cell Events: 6444 mean_circ: 0.953036
Ran for 192550 frames Cell Events: 6463 mean_circ: 0.953037
Ran for 193050 frames Cell Events: 6489 mean_circ: 0.952971
Ran for 194050 frames Cell Events: 6540 mean_circ: 0.952988
Ran for 195050 frames Cell Events: 6595 mean_circ: 0.952998
Ran for 196550 frames Cell Events: 6685 mean_circ: 0.953059
Ran for 197550 frames Cell Events: 6724 mean_circ: 0.95307
Ran for 198550 frames Cell Events: 6771 mean_circ: 0.953129
Ran for 199050 frames Cell Events: 6788 mean_circ: 0.953155
Ran for 199550 frames Cell Events: 6810 mean_circ: 0.953189
Ran for 201550 frames Cell Events: 6903 mean_circ: 0.953259
Ran for 202050 frames Cell Events: 6918 mean_circ: 0.953242
Ran for 202550 frames Cell Events: 6941 mean_circ: 0.953264
Ran for 203050 frames Cell Events: 6971 mean_circ: 0.953265
Ran for 204050 frames Cell Events: 7006 mean_circ: 0.953304
Ran for 204550 frames Cell Events: 7030 mean_circ: 0.953305
Ran for 206050 frames Cell Events: 7118 mean_circ: 0.953267
Ran for 206550 frames Cell Events: 7153 mean_circ: 0.953249
Ran for 207050 frames Cell Events: 7177 mean_circ: 0.95329
Ran for 208050 frames Cell Events: 7231 mean_circ: 0.953299
Ran for 208550 frames Cell Events: 7250 mean_circ: 0.953287
Ran for 209050 frames Cell Events: 7269 mean_circ: 0.953306
Ran for 209550 frames Cell Events: 7285 mean_circ: 0.953336
Ran for 210050 frames Cell Events: 7310 mean_circ: 0.953373
Ran for 210550 frames Cell Events: 7331 mean_circ: 0.953338
Ran for 211050 frames Cell Events: 7352 mean_circ: 0.953329
Ran for 213550 frames Cell Events: 7471 mean_circ: 0.953376
Ran for 214550 frames Cell Events: 7515 mean_circ: 0.9534
Ran for 215050 frames Cell Events: 7543 mean_circ: 0.953354
Ran for 215550 frames Cell Events: 7557 mean_circ: 0.953341
Ran for 216050 frames Cell Events: 7581 mean_circ: 0.953341
Ran for 217550 frames Cell Events: 7658 mean_circ: 0.953379
Ran for 219050 frames Cell Events: 7732 mean_circ: 0.953393
Ran for 219550 frames Cell Events: 7766 mean_circ: 0.953381
Ran for 220050 frames Cell Events: 7790 mean_circ: 0.953382
Ran for 221050 frames Cell Events: 7844 mean_circ: 0.953421
Ran for 222050 frames Cell Events: 7893 mean_circ: 0.953364
Ran for 223050 frames Cell Events: 7937 mean_circ: 0.953423
Ran for 223550 frames Cell Events: 7963 mean_circ: 0.953422
End this measurment..
Stopping time: 2.90571e+008
****************************************
Measurement finished
Mean Circularitiy: 0.953423
Mean Area: 617.148
Mean FL-1 intensity: 2746.07
Mean FL-2 intensity: 4808.94
Mean FL-3 intensity: 0
frames processed: 223903
Total Time: 112078ms
Number of Runs (new bg img) 0
Frames collected: 223957
Skipped frames: 52257
Rescued Frames: 2897459
Samples DAQ: 55992423
Samples written: 111978458
Samples per Channel: 55989229
FPS: 1998.22
Samples per second: 499555
Expected samples/s: 500000
Events: 7989
Events per second: 71.2806
jumped back (PMT): 4524
#Frames read, frequency
# 0 frames :310644
# 1 frames :121500
# 2 frames :4260
# 3 frames :1662
# 4 frames :862
# 5 frames :248
# 6 frames :1565
# 7 frames :1787
# 8 frames :960
# 9 frames :607
# 10 frames :1209
# 11 frames :434
# 12 frames :465
# 13 frames :240
# 14 frames :223
# 15 frames :229
# 16 frames :170
# 17 frames :161
# 18 frames :101
# 19 frames :59
# 20 frames :41
# 21 frames :65
# 22 frames :35
# 23 frames :36
# 24 frames :30
# 25 frames :18
# 26 frames :15
# 27 frames :18
# 28 frames :8
# 29 frames :12
# 30 frames :14
# 31 frames :7
# 32 frames :10
# 33 frames :5
# 34 frames :6
# 35 frames :4
# 36 frames :3
# 37 frames :4
# 40 frames :3
# 42 frames :3
# 44 frames :1
# 45 frames :1
# 46 frames :1
# 47 frames :1
# 49 frames :1
# 50 frames :1
# 54 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 27.379
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0