Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_11.56.47
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "372a1bf7befc7e4cf6d7a85ba87caef9",
"name": "M1_20170530_2034_c2_dsRNA_K7_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "09:57:08"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8033 (max 8034) in '8033'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9307
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.33991e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 37 mean_circ: 0.945374
Ran for 1550 frames Cell Events: 54 mean_circ: 0.950285
Ran for 2550 frames Cell Events: 74 mean_circ: 0.94523
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Ran for 5050 frames Cell Events: 145 mean_circ: 0.951165
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Ran for 7550 frames Cell Events: 218 mean_circ: 0.94824
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Ran for 87050 frames Cell Events: 2574 mean_circ: 0.94307
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Ran for 91050 frames Cell Events: 2695 mean_circ: 0.943198
Ran for 92050 frames Cell Events: 2733 mean_circ: 0.943288
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Ran for 93050 frames Cell Events: 2760 mean_circ: 0.943401
Ran for 94050 frames Cell Events: 2794 mean_circ: 0.943297
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Ran for 97050 frames Cell Events: 2878 mean_circ: 0.94316
Ran for 97550 frames Cell Events: 2893 mean_circ: 0.943201
Ran for 98050 frames Cell Events: 2905 mean_circ: 0.943232
Ran for 99050 frames Cell Events: 2930 mean_circ: 0.943005
Ran for 99550 frames Cell Events: 2941 mean_circ: 0.943011
Ran for 100050 frames Cell Events: 2951 mean_circ: 0.943023
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Ran for 102550 frames Cell Events: 3025 mean_circ: 0.943124
Ran for 103550 frames Cell Events: 3059 mean_circ: 0.943052
Ran for 104050 frames Cell Events: 3065 mean_circ: 0.943047
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Ran for 107550 frames Cell Events: 3181 mean_circ: 0.94309
Ran for 108050 frames Cell Events: 3192 mean_circ: 0.943125
Ran for 108550 frames Cell Events: 3206 mean_circ: 0.943192
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Ran for 123050 frames Cell Events: 3693 mean_circ: 0.942959
Ran for 123550 frames Cell Events: 3710 mean_circ: 0.942988
Ran for 124050 frames Cell Events: 3725 mean_circ: 0.943018
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Ran for 125050 frames Cell Events: 3753 mean_circ: 0.943074
Ran for 126050 frames Cell Events: 3790 mean_circ: 0.94304
Ran for 126550 frames Cell Events: 3816 mean_circ: 0.943016
Ran for 127050 frames Cell Events: 3834 mean_circ: 0.942942
Ran for 128050 frames Cell Events: 3852 mean_circ: 0.942881
Ran for 128550 frames Cell Events: 3867 mean_circ: 0.942896
Ran for 129050 frames Cell Events: 3883 mean_circ: 0.942893
Ran for 131050 frames Cell Events: 3950 mean_circ: 0.94287
Ran for 131550 frames Cell Events: 3970 mean_circ: 0.942958
Ran for 132050 frames Cell Events: 3979 mean_circ: 0.942996
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Ran for 147550 frames Cell Events: 4495 mean_circ: 0.942709
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Ran for 212550 frames Cell Events: 6704 mean_circ: 0.941655
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Ran for 213550 frames Cell Events: 6732 mean_circ: 0.941625
Ran for 214050 frames Cell Events: 6752 mean_circ: 0.941571
Ran for 216050 frames Cell Events: 6828 mean_circ: 0.941556
Ran for 217050 frames Cell Events: 6862 mean_circ: 0.941585
Ran for 218050 frames Cell Events: 6893 mean_circ: 0.94154
Ran for 218550 frames Cell Events: 6915 mean_circ: 0.941582
Ran for 219050 frames Cell Events: 6937 mean_circ: 0.941565
Ran for 221050 frames Cell Events: 7005 mean_circ: 0.941573
Ran for 222050 frames Cell Events: 7023 mean_circ: 0.941548
Ran for 222550 frames Cell Events: 7034 mean_circ: 0.941534
Ran for 223050 frames Cell Events: 7060 mean_circ: 0.941536
Ran for 223550 frames Cell Events: 7076 mean_circ: 0.941499
Ran for 224050 frames Cell Events: 7100 mean_circ: 0.941467
Ran for 224550 frames Cell Events: 7112 mean_circ: 0.941484
Ran for 226050 frames Cell Events: 7160 mean_circ: 0.941495
Ran for 226550 frames Cell Events: 7177 mean_circ: 0.941443
Ran for 227050 frames Cell Events: 7193 mean_circ: 0.941444
Ran for 228050 frames Cell Events: 7225 mean_circ: 0.941328
Ran for 228550 frames Cell Events: 7236 mean_circ: 0.941336
Ran for 230050 frames Cell Events: 7299 mean_circ: 0.941325
Ran for 230550 frames Cell Events: 7313 mean_circ: 0.941328
Ran for 231050 frames Cell Events: 7322 mean_circ: 0.941349
Ran for 231550 frames Cell Events: 7335 mean_circ: 0.941361
Ran for 232050 frames Cell Events: 7348 mean_circ: 0.941325
Ran for 232550 frames Cell Events: 7363 mean_circ: 0.941319
Ran for 233550 frames Cell Events: 7379 mean_circ: 0.941322
Ran for 234050 frames Cell Events: 7390 mean_circ: 0.94134
Ran for 234550 frames Cell Events: 7408 mean_circ: 0.941351
Ran for 235050 frames Cell Events: 7425 mean_circ: 0.941327
Ran for 235550 frames Cell Events: 7441 mean_circ: 0.941295
Ran for 236050 frames Cell Events: 7452 mean_circ: 0.941325
Ran for 238050 frames Cell Events: 7532 mean_circ: 0.941271
Ran for 238550 frames Cell Events: 7543 mean_circ: 0.94128
Ran for 239050 frames Cell Events: 7561 mean_circ: 0.941265
Ran for 240550 frames Cell Events: 7608 mean_circ: 0.94129
Ran for 241050 frames Cell Events: 7621 mean_circ: 0.941303
Ran for 242550 frames Cell Events: 7672 mean_circ: 0.941226
Ran for 243050 frames Cell Events: 7685 mean_circ: 0.941231
Ran for 243550 frames Cell Events: 7696 mean_circ: 0.941212
Ran for 244050 frames Cell Events: 7706 mean_circ: 0.94123
Ran for 244550 frames Cell Events: 7713 mean_circ: 0.94123
Ran for 245050 frames Cell Events: 7727 mean_circ: 0.941249
Ran for 245550 frames Cell Events: 7742 mean_circ: 0.941263
Ran for 246050 frames Cell Events: 7759 mean_circ: 0.941217
Ran for 246550 frames Cell Events: 7770 mean_circ: 0.941232
Ran for 247550 frames Cell Events: 7795 mean_circ: 0.941218
Ran for 248550 frames Cell Events: 7821 mean_circ: 0.941244
Ran for 249050 frames Cell Events: 7834 mean_circ: 0.941262
Ran for 249550 frames Cell Events: 7853 mean_circ: 0.941311
Ran for 250550 frames Cell Events: 7876 mean_circ: 0.941312
Ran for 251050 frames Cell Events: 7891 mean_circ: 0.941324
Ran for 251550 frames Cell Events: 7910 mean_circ: 0.941324
Ran for 252050 frames Cell Events: 7921 mean_circ: 0.94134
Ran for 253050 frames Cell Events: 7956 mean_circ: 0.941345
Ran for 254050 frames Cell Events: 8001 mean_circ: 0.941327
Ran for 254550 frames Cell Events: 8020 mean_circ: 0.941334
End this measurment..
Stopping time: 1.34004e+009
****************************************
Measurement finished
Mean Circularitiy: 0.94131
Mean Area: 604.334
Mean FL-1 intensity: 2687.33
Mean FL-2 intensity: 3990.4
Mean FL-3 intensity: 0
frames processed: 251906
Total Time: 127558ms
Number of Runs (new bg img) 0
Frames collected: 254955
Skipped frames: 96144
Rescued Frames: 4620711
Samples DAQ: 63739893
Samples written: 127477332
Samples per Channel: 63738666
FPS: 1998.73
Samples per second: 499683
Expected samples/s: 500000
Events: 8034
Events per second: 62.983
jumped back (PMT): 4610
#Frames read, frequency
# 0 frames :359524
# 1 frames :132733
# 2 frames :4774
# 3 frames :1827
# 4 frames :988
# 5 frames :525
# 6 frames :1607
# 7 frames :1780
# 8 frames :1415
# 9 frames :925
# 10 frames :616
# 11 frames :450
# 12 frames :426
# 13 frames :323
# 14 frames :306
# 15 frames :252
# 16 frames :209
# 17 frames :170
# 18 frames :129
# 19 frames :116
# 20 frames :98
# 21 frames :96
# 22 frames :83
# 23 frames :65
# 24 frames :44
# 25 frames :41
# 26 frames :39
# 27 frames :27
# 28 frames :18
# 29 frames :27
# 30 frames :14
# 31 frames :14
# 32 frames :13
# 33 frames :11
# 34 frames :9
# 35 frames :13
# 36 frames :5
# 37 frames :7
# 38 frames :5
# 39 frames :6
# 40 frames :6
# 41 frames :1
# 42 frames :5
# 45 frames :4
# 46 frames :1
# 47 frames :1
# 48 frames :1
# 49 frames :2
# 51 frames :3
# 53 frames :1
# 54 frames :1
# 59 frames :1
# 66 frames :1
# 78 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9307
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0