Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_18.29.36
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "ca67c55f5ba8b504dda8d166904f9ce6",
"name": "M1_20170519_1450_dsRNA_L1_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "16:30:07"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9380 (max 9382) in '9380'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.613
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.6889e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 17 mean_circ: 0.893267
Ran for 1050 frames Cell Events: 29 mean_circ: 0.910168
Ran for 2050 frames Cell Events: 52 mean_circ: 0.923989
Ran for 2550 frames Cell Events: 65 mean_circ: 0.927865
Ran for 3050 frames Cell Events: 77 mean_circ: 0.931051
Ran for 4550 frames Cell Events: 128 mean_circ: 0.938308
Ran for 5050 frames Cell Events: 142 mean_circ: 0.936531
Ran for 5550 frames Cell Events: 155 mean_circ: 0.93655
Ran for 6050 frames Cell Events: 162 mean_circ: 0.937654
Ran for 7050 frames Cell Events: 187 mean_circ: 0.938841
Ran for 7550 frames Cell Events: 203 mean_circ: 0.93759
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Ran for 9050 frames Cell Events: 239 mean_circ: 0.937704
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Ran for 10550 frames Cell Events: 269 mean_circ: 0.937862
Ran for 11050 frames Cell Events: 285 mean_circ: 0.938581
Ran for 12550 frames Cell Events: 329 mean_circ: 0.938411
Ran for 13050 frames Cell Events: 344 mean_circ: 0.937905
Ran for 13550 frames Cell Events: 361 mean_circ: 0.937086
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Ran for 15550 frames Cell Events: 412 mean_circ: 0.937104
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Ran for 64550 frames Cell Events: 2002 mean_circ: 0.941687
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Ran for 65550 frames Cell Events: 2042 mean_circ: 0.941838
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Ran for 68550 frames Cell Events: 2128 mean_circ: 0.942036
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Ran for 77050 frames Cell Events: 2377 mean_circ: 0.941793
Ran for 77550 frames Cell Events: 2393 mean_circ: 0.941799
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Ran for 80550 frames Cell Events: 2496 mean_circ: 0.941322
Ran for 81050 frames Cell Events: 2516 mean_circ: 0.941219
Ran for 81550 frames Cell Events: 2535 mean_circ: 0.94129
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Ran for 83050 frames Cell Events: 2580 mean_circ: 0.941351
Ran for 83550 frames Cell Events: 2594 mean_circ: 0.941364
Ran for 84050 frames Cell Events: 2612 mean_circ: 0.941396
Ran for 84550 frames Cell Events: 2637 mean_circ: 0.941469
Ran for 85050 frames Cell Events: 2649 mean_circ: 0.941517
Ran for 86050 frames Cell Events: 2681 mean_circ: 0.94153
Ran for 87550 frames Cell Events: 2738 mean_circ: 0.941562
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Ran for 89050 frames Cell Events: 2783 mean_circ: 0.941511
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Ran for 91050 frames Cell Events: 2858 mean_circ: 0.941235
Ran for 91550 frames Cell Events: 2879 mean_circ: 0.941276
Ran for 92050 frames Cell Events: 2898 mean_circ: 0.941367
Ran for 92550 frames Cell Events: 2911 mean_circ: 0.941266
Ran for 93050 frames Cell Events: 2929 mean_circ: 0.941253
Ran for 93550 frames Cell Events: 2945 mean_circ: 0.941306
Ran for 94050 frames Cell Events: 2964 mean_circ: 0.941392
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Ran for 95050 frames Cell Events: 3011 mean_circ: 0.941371
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Ran for 97050 frames Cell Events: 3086 mean_circ: 0.941099
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Ran for 102550 frames Cell Events: 3302 mean_circ: 0.941195
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Ran for 104050 frames Cell Events: 3350 mean_circ: 0.941224
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Ran for 106050 frames Cell Events: 3425 mean_circ: 0.941248
Ran for 107550 frames Cell Events: 3484 mean_circ: 0.941305
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Ran for 112050 frames Cell Events: 3650 mean_circ: 0.941509
Ran for 113550 frames Cell Events: 3707 mean_circ: 0.941589
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Ran for 114550 frames Cell Events: 3740 mean_circ: 0.941577
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Ran for 121550 frames Cell Events: 3987 mean_circ: 0.941661
Ran for 122550 frames Cell Events: 4023 mean_circ: 0.941521
Ran for 123050 frames Cell Events: 4038 mean_circ: 0.941452
Ran for 123550 frames Cell Events: 4055 mean_circ: 0.941406
Ran for 124050 frames Cell Events: 4077 mean_circ: 0.941396
Ran for 124550 frames Cell Events: 4088 mean_circ: 0.941401
Ran for 125050 frames Cell Events: 4111 mean_circ: 0.941404
Ran for 126550 frames Cell Events: 4180 mean_circ: 0.941381
Ran for 127050 frames Cell Events: 4201 mean_circ: 0.941399
Ran for 128050 frames Cell Events: 4245 mean_circ: 0.941416
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Ran for 130050 frames Cell Events: 4320 mean_circ: 0.94151
Ran for 130550 frames Cell Events: 4336 mean_circ: 0.941511
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Ran for 135050 frames Cell Events: 4506 mean_circ: 0.941593
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Ran for 138050 frames Cell Events: 4638 mean_circ: 0.941534
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Ran for 142050 frames Cell Events: 4789 mean_circ: 0.941567
Ran for 142550 frames Cell Events: 4807 mean_circ: 0.941573
Ran for 143050 frames Cell Events: 4829 mean_circ: 0.941509
Ran for 143550 frames Cell Events: 4848 mean_circ: 0.941533
Ran for 144050 frames Cell Events: 4863 mean_circ: 0.941537
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Ran for 148050 frames Cell Events: 4998 mean_circ: 0.941606
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Ran for 152550 frames Cell Events: 5187 mean_circ: 0.941575
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Ran for 173550 frames Cell Events: 6015 mean_circ: 0.941061
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Ran for 176550 frames Cell Events: 6145 mean_circ: 0.941013
Ran for 177050 frames Cell Events: 6164 mean_circ: 0.941037
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Ran for 182550 frames Cell Events: 6372 mean_circ: 0.941129
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Ran for 192050 frames Cell Events: 6711 mean_circ: 0.940984
Ran for 192550 frames Cell Events: 6730 mean_circ: 0.940983
Ran for 193050 frames Cell Events: 6741 mean_circ: 0.940981
Ran for 194050 frames Cell Events: 6774 mean_circ: 0.940972
Ran for 194550 frames Cell Events: 6788 mean_circ: 0.941012
Ran for 195550 frames Cell Events: 6821 mean_circ: 0.94099
Ran for 196050 frames Cell Events: 6831 mean_circ: 0.940973
Ran for 196550 frames Cell Events: 6853 mean_circ: 0.941008
Ran for 197550 frames Cell Events: 6889 mean_circ: 0.940974
Ran for 198050 frames Cell Events: 6905 mean_circ: 0.940941
Ran for 198550 frames Cell Events: 6922 mean_circ: 0.940947
Ran for 199050 frames Cell Events: 6937 mean_circ: 0.940923
Ran for 199550 frames Cell Events: 6951 mean_circ: 0.940926
Ran for 200050 frames Cell Events: 6964 mean_circ: 0.940925
Ran for 200550 frames Cell Events: 6985 mean_circ: 0.940982
Ran for 201050 frames Cell Events: 7007 mean_circ: 0.940975
Ran for 202050 frames Cell Events: 7046 mean_circ: 0.940955
Ran for 203050 frames Cell Events: 7081 mean_circ: 0.94096
Ran for 203550 frames Cell Events: 7097 mean_circ: 0.940966
Ran for 204050 frames Cell Events: 7122 mean_circ: 0.941029
Ran for 204550 frames Cell Events: 7137 mean_circ: 0.941049
Ran for 205050 frames Cell Events: 7151 mean_circ: 0.941038
Ran for 206050 frames Cell Events: 7202 mean_circ: 0.941103
Ran for 206550 frames Cell Events: 7226 mean_circ: 0.941145
Ran for 207050 frames Cell Events: 7245 mean_circ: 0.941165
Ran for 207550 frames Cell Events: 7261 mean_circ: 0.941141
Ran for 208050 frames Cell Events: 7286 mean_circ: 0.941177
Ran for 208550 frames Cell Events: 7310 mean_circ: 0.941139
Ran for 209050 frames Cell Events: 7332 mean_circ: 0.941122
Ran for 210050 frames Cell Events: 7372 mean_circ: 0.941085
Ran for 211550 frames Cell Events: 7440 mean_circ: 0.941096
Ran for 212050 frames Cell Events: 7459 mean_circ: 0.941076
Ran for 212550 frames Cell Events: 7486 mean_circ: 0.94109
Ran for 213050 frames Cell Events: 7509 mean_circ: 0.941094
Ran for 213550 frames Cell Events: 7522 mean_circ: 0.941062
Ran for 214050 frames Cell Events: 7536 mean_circ: 0.941093
Ran for 214550 frames Cell Events: 7556 mean_circ: 0.941102
Ran for 215050 frames Cell Events: 7576 mean_circ: 0.941142
Ran for 215550 frames Cell Events: 7601 mean_circ: 0.941102
Ran for 216550 frames Cell Events: 7646 mean_circ: 0.94106
Ran for 219050 frames Cell Events: 7760 mean_circ: 0.941043
Ran for 219550 frames Cell Events: 7782 mean_circ: 0.941066
Ran for 220050 frames Cell Events: 7804 mean_circ: 0.941003
Ran for 221050 frames Cell Events: 7849 mean_circ: 0.941003
Ran for 221550 frames Cell Events: 7868 mean_circ: 0.941031
Ran for 222050 frames Cell Events: 7878 mean_circ: 0.940992
Ran for 222550 frames Cell Events: 7893 mean_circ: 0.941011
Ran for 223050 frames Cell Events: 7916 mean_circ: 0.941056
Ran for 223550 frames Cell Events: 7929 mean_circ: 0.941018
Ran for 224550 frames Cell Events: 7978 mean_circ: 0.941016
Ran for 225050 frames Cell Events: 7996 mean_circ: 0.941017
Ran for 225550 frames Cell Events: 8019 mean_circ: 0.941006
Ran for 226050 frames Cell Events: 8032 mean_circ: 0.941016
Ran for 226550 frames Cell Events: 8046 mean_circ: 0.94101
Ran for 228550 frames Cell Events: 8121 mean_circ: 0.940922
Ran for 229050 frames Cell Events: 8145 mean_circ: 0.940896
Ran for 229550 frames Cell Events: 8162 mean_circ: 0.940904
Ran for 230550 frames Cell Events: 8191 mean_circ: 0.940912
Ran for 231550 frames Cell Events: 8229 mean_circ: 0.940881
Ran for 232050 frames Cell Events: 8246 mean_circ: 0.940865
Ran for 233550 frames Cell Events: 8304 mean_circ: 0.940814
Ran for 234050 frames Cell Events: 8322 mean_circ: 0.940819
Ran for 234550 frames Cell Events: 8348 mean_circ: 0.940834
Ran for 235050 frames Cell Events: 8365 mean_circ: 0.940853
Ran for 235550 frames Cell Events: 8386 mean_circ: 0.940886
Ran for 236050 frames Cell Events: 8408 mean_circ: 0.940883
Ran for 236550 frames Cell Events: 8428 mean_circ: 0.940875
Ran for 237550 frames Cell Events: 8474 mean_circ: 0.94086
Ran for 238050 frames Cell Events: 8502 mean_circ: 0.940843
Ran for 238550 frames Cell Events: 8518 mean_circ: 0.940824
Ran for 239050 frames Cell Events: 8527 mean_circ: 0.940803
Ran for 239550 frames Cell Events: 8553 mean_circ: 0.940811
Ran for 240050 frames Cell Events: 8575 mean_circ: 0.940823
Ran for 241050 frames Cell Events: 8626 mean_circ: 0.940821
Ran for 242550 frames Cell Events: 8697 mean_circ: 0.940864
Ran for 243050 frames Cell Events: 8713 mean_circ: 0.940884
Ran for 244050 frames Cell Events: 8754 mean_circ: 0.940924
Ran for 244550 frames Cell Events: 8772 mean_circ: 0.940875
Ran for 246050 frames Cell Events: 8835 mean_circ: 0.940935
Ran for 246550 frames Cell Events: 8864 mean_circ: 0.940935
Ran for 247550 frames Cell Events: 8910 mean_circ: 0.940933
Ran for 248050 frames Cell Events: 8931 mean_circ: 0.940935
Ran for 249050 frames Cell Events: 8974 mean_circ: 0.940887
Ran for 250050 frames Cell Events: 9034 mean_circ: 0.940891
Ran for 251050 frames Cell Events: 9065 mean_circ: 0.940909
Ran for 251550 frames Cell Events: 9073 mean_circ: 0.940921
Ran for 252550 frames Cell Events: 9111 mean_circ: 0.940918
Ran for 253050 frames Cell Events: 9128 mean_circ: 0.940942
Ran for 253550 frames Cell Events: 9156 mean_circ: 0.940938
Ran for 254050 frames Cell Events: 9182 mean_circ: 0.940937
Ran for 255050 frames Cell Events: 9218 mean_circ: 0.940915
Ran for 256050 frames Cell Events: 9233 mean_circ: 0.94094
Ran for 256550 frames Cell Events: 9245 mean_circ: 0.940942
Ran for 257050 frames Cell Events: 9262 mean_circ: 0.940943
Ran for 257550 frames Cell Events: 9280 mean_circ: 0.940934
Ran for 258050 frames Cell Events: 9294 mean_circ: 0.940933
Ran for 259050 frames Cell Events: 9322 mean_circ: 0.940968
Ran for 259550 frames Cell Events: 9340 mean_circ: 0.940955
Ran for 260050 frames Cell Events: 9360 mean_circ: 0.940941
Ran for 260550 frames Cell Events: 9380 mean_circ: 0.940949
End this measurment..
Stopping time: 3.6902e+008
****************************************
Measurement finished
Mean Circularitiy: 0.94095
Mean Area: 606.613
Mean FL-1 intensity: 3302.9
Mean FL-2 intensity: 4392.99
Mean FL-3 intensity: 0
frames processed: 257979
Total Time: 130398ms
Number of Runs (new bg img) 0
Frames collected: 260645
Skipped frames: 34113
Rescued Frames: 1659886
Samples DAQ: 65161254
Samples written: 130322290
Samples per Channel: 65161145
FPS: 1998.84
Samples per second: 499710
Expected samples/s: 500000
Events: 9382
Events per second: 71.9489
jumped back (PMT): 5929
#Frames read, frequency
# 0 frames :358548
# 1 frames :143848
# 2 frames :5537
# 3 frames :1717
# 4 frames :888
# 5 frames :319
# 6 frames :2808
# 7 frames :2490
# 8 frames :1418
# 9 frames :819
# 10 frames :681
# 11 frames :375
# 12 frames :378
# 13 frames :297
# 14 frames :233
# 15 frames :209
# 16 frames :155
# 17 frames :114
# 18 frames :78
# 19 frames :63
# 20 frames :37
# 21 frames :56
# 22 frames :35
# 23 frames :37
# 24 frames :19
# 25 frames :29
# 26 frames :6
# 27 frames :9
# 28 frames :7
# 29 frames :10
# 30 frames :10
# 31 frames :6
# 32 frames :4
# 33 frames :5
# 34 frames :2
# 35 frames :1
# 36 frames :1
# 38 frames :4
# 39 frames :1
# 40 frames :2
# 41 frames :1
# 44 frames :2
# 47 frames :1
# 50 frames :1
# 52 frames :1
# 67 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.613
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0