20170519_1643_phos1_M1.rtdc
Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_18.41.07
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}dclab-tdms2rtdc
{"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "c0db0a470d003142079d219b3ba42f9c",
"name": "M1_20170519_1643_dsRNA_M1_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "16:41:41"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarningin dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9999 (max 10000) in
'9999'.
FG_Config.mcf
[Kamerasektion]ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.5874
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.75642e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 5 mean_circ: 0.948984
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Ran for 391050 frames Cell Events: 7854 mean_circ: 0.927794
Ran for 391550 frames Cell Events: 7866 mean_circ: 0.927799
Ran for 392050 frames Cell Events: 7878 mean_circ: 0.927796
Ran for 392550 frames Cell Events: 7893 mean_circ: 0.927798
Ran for 393050 frames Cell Events: 7906 mean_circ: 0.9278
Ran for 394050 frames Cell Events: 7925 mean_circ: 0.927789
Ran for 394550 frames Cell Events: 7938 mean_circ: 0.927811
Ran for 395050 frames Cell Events: 7943 mean_circ: 0.927824
Ran for 395550 frames Cell Events: 7952 mean_circ: 0.927832
Ran for 396050 frames Cell Events: 7960 mean_circ: 0.927839
Ran for 396550 frames Cell Events: 7967 mean_circ: 0.927833
Ran for 397050 frames Cell Events: 7982 mean_circ: 0.92784
Ran for 397550 frames Cell Events: 7991 mean_circ: 0.927802
Ran for 398050 frames Cell Events: 7995 mean_circ: 0.927791
Ran for 398550 frames Cell Events: 8001 mean_circ: 0.92778
Ran for 399050 frames Cell Events: 8010 mean_circ: 0.927766
Ran for 399550 frames Cell Events: 8016 mean_circ: 0.927788
Ran for 400550 frames Cell Events: 8033 mean_circ: 0.927743
Ran for 401550 frames Cell Events: 8059 mean_circ: 0.927713
Ran for 402050 frames Cell Events: 8067 mean_circ: 0.927721
Ran for 402550 frames Cell Events: 8077 mean_circ: 0.927748
Ran for 403550 frames Cell Events: 8095 mean_circ: 0.927694
Ran for 404050 frames Cell Events: 8109 mean_circ: 0.927717
Ran for 404550 frames Cell Events: 8122 mean_circ: 0.927673
Ran for 405050 frames Cell Events: 8135 mean_circ: 0.927688
Ran for 405550 frames Cell Events: 8142 mean_circ: 0.927672
Ran for 406050 frames Cell Events: 8158 mean_circ: 0.92767
Ran for 406550 frames Cell Events: 8170 mean_circ: 0.927671
Ran for 407050 frames Cell Events: 8177 mean_circ: 0.927676
Ran for 407550 frames Cell Events: 8189 mean_circ: 0.927667
Ran for 408050 frames Cell Events: 8201 mean_circ: 0.927664
Ran for 408550 frames Cell Events: 8210 mean_circ: 0.927666
Ran for 409050 frames Cell Events: 8221 mean_circ: 0.927622
Ran for 409550 frames Cell Events: 8236 mean_circ: 0.927625
Ran for 410050 frames Cell Events: 8251 mean_circ: 0.927615
Ran for 410550 frames Cell Events: 8259 mean_circ: 0.927603
Ran for 411050 frames Cell Events: 8265 mean_circ: 0.92759
Ran for 411550 frames Cell Events: 8276 mean_circ: 0.927602
Ran for 412050 frames Cell Events: 8288 mean_circ: 0.927645
Ran for 412550 frames Cell Events: 8297 mean_circ: 0.927649
Ran for 413550 frames Cell Events: 8322 mean_circ: 0.927648
Ran for 414050 frames Cell Events: 8330 mean_circ: 0.927642
Ran for 414550 frames Cell Events: 8338 mean_circ: 0.92763
Ran for 415050 frames Cell Events: 8346 mean_circ: 0.927641
Ran for 415550 frames Cell Events: 8355 mean_circ: 0.92764
Ran for 416050 frames Cell Events: 8359 mean_circ: 0.92764
Ran for 416550 frames Cell Events: 8367 mean_circ: 0.927644
Ran for 417050 frames Cell Events: 8380 mean_circ: 0.92762
Ran for 417550 frames Cell Events: 8382 mean_circ: 0.927625
Ran for 418050 frames Cell Events: 8389 mean_circ: 0.927628
Ran for 418550 frames Cell Events: 8392 mean_circ: 0.927635
Ran for 419050 frames Cell Events: 8407 mean_circ: 0.927639
Ran for 419550 frames Cell Events: 8416 mean_circ: 0.927629
Ran for 420050 frames Cell Events: 8422 mean_circ: 0.927645
Ran for 420550 frames Cell Events: 8431 mean_circ: 0.927641
Ran for 421050 frames Cell Events: 8443 mean_circ: 0.927646
Ran for 421550 frames Cell Events: 8454 mean_circ: 0.927656
Ran for 422050 frames Cell Events: 8463 mean_circ: 0.927628
Ran for 422550 frames Cell Events: 8469 mean_circ: 0.927627
Ran for 423050 frames Cell Events: 8481 mean_circ: 0.927618
Ran for 423550 frames Cell Events: 8486 mean_circ: 0.927622
Ran for 424550 frames Cell Events: 8506 mean_circ: 0.927566
Ran for 425050 frames Cell Events: 8510 mean_circ: 0.927572
Ran for 425550 frames Cell Events: 8521 mean_circ: 0.927566
Ran for 426050 frames Cell Events: 8528 mean_circ: 0.927582
Ran for 426550 frames Cell Events: 8541 mean_circ: 0.927588
Ran for 427050 frames Cell Events: 8548 mean_circ: 0.927602
Ran for 428050 frames Cell Events: 8572 mean_circ: 0.927589
Ran for 429050 frames Cell Events: 8591 mean_circ: 0.927622
Ran for 429550 frames Cell Events: 8600 mean_circ: 0.927624
Ran for 430050 frames Cell Events: 8609 mean_circ: 0.927598
Ran for 430550 frames Cell Events: 8625 mean_circ: 0.927602
Ran for 431050 frames Cell Events: 8631 mean_circ: 0.927603
Ran for 431550 frames Cell Events: 8644 mean_circ: 0.927615
Ran for 432050 frames Cell Events: 8659 mean_circ: 0.927582
Ran for 432550 frames Cell Events: 8669 mean_circ: 0.927581
Ran for 433050 frames Cell Events: 8679 mean_circ: 0.927536
Ran for 433550 frames Cell Events: 8687 mean_circ: 0.927546
Ran for 434550 frames Cell Events: 8701 mean_circ: 0.92757
Ran for 435050 frames Cell Events: 8710 mean_circ: 0.927589
Ran for 435550 frames Cell Events: 8722 mean_circ: 0.92759
Ran for 436050 frames Cell Events: 8730 mean_circ: 0.927584
Ran for 436550 frames Cell Events: 8741 mean_circ: 0.927601
Ran for 437050 frames Cell Events: 8750 mean_circ: 0.927583
Ran for 437550 frames Cell Events: 8775 mean_circ: 0.927588
Ran for 438550 frames Cell Events: 8799 mean_circ: 0.927576
Ran for 439050 frames Cell Events: 8811 mean_circ: 0.927597
Ran for 439550 frames Cell Events: 8827 mean_circ: 0.92761
Ran for 440050 frames Cell Events: 8832 mean_circ: 0.927606
Ran for 440550 frames Cell Events: 8839 mean_circ: 0.927615
Ran for 441050 frames Cell Events: 8847 mean_circ: 0.927639
Ran for 441550 frames Cell Events: 8853 mean_circ: 0.92763
Ran for 442550 frames Cell Events: 8872 mean_circ: 0.927651
Ran for 443550 frames Cell Events: 8887 mean_circ: 0.92767
Ran for 444050 frames Cell Events: 8900 mean_circ: 0.927677
Ran for 444550 frames Cell Events: 8905 mean_circ: 0.927697
Ran for 445050 frames Cell Events: 8917 mean_circ: 0.927696
Ran for 445550 frames Cell Events: 8921 mean_circ: 0.92769
Ran for 446550 frames Cell Events: 8940 mean_circ: 0.92769
Ran for 447050 frames Cell Events: 8953 mean_circ: 0.927685
Ran for 447550 frames Cell Events: 8960 mean_circ: 0.927666
Ran for 448050 frames Cell Events: 8971 mean_circ: 0.927665
Ran for 449050 frames Cell Events: 8988 mean_circ: 0.927612
Ran for 450050 frames Cell Events: 9011 mean_circ: 0.927539
Ran for 451050 frames Cell Events: 9027 mean_circ: 0.927513
Ran for 451550 frames Cell Events: 9034 mean_circ: 0.927478
Ran for 452050 frames Cell Events: 9045 mean_circ: 0.92748
Ran for 452550 frames Cell Events: 9059 mean_circ: 0.927502
Ran for 453050 frames Cell Events: 9066 mean_circ: 0.927491
Ran for 453550 frames Cell Events: 9077 mean_circ: 0.92749
Ran for 454550 frames Cell Events: 9101 mean_circ: 0.927498
Ran for 455050 frames Cell Events: 9106 mean_circ: 0.927516
Ran for 455550 frames Cell Events: 9120 mean_circ: 0.927535
Ran for 456050 frames Cell Events: 9127 mean_circ: 0.927542
Ran for 456550 frames Cell Events: 9147 mean_circ: 0.927474
Ran for 457050 frames Cell Events: 9161 mean_circ: 0.927496
Ran for 457550 frames Cell Events: 9175 mean_circ: 0.927446
Ran for 458050 frames Cell Events: 9188 mean_circ: 0.927459
Ran for 458550 frames Cell Events: 9206 mean_circ: 0.927514
Ran for 459050 frames Cell Events: 9217 mean_circ: 0.927512
Ran for 459550 frames Cell Events: 9232 mean_circ: 0.927522
Ran for 460050 frames Cell Events: 9241 mean_circ: 0.927538
Ran for 461050 frames Cell Events: 9274 mean_circ: 0.927573
Ran for 461550 frames Cell Events: 9281 mean_circ: 0.927596
Ran for 462050 frames Cell Events: 9288 mean_circ: 0.927606
Ran for 462550 frames Cell Events: 9305 mean_circ: 0.927633
Ran for 463050 frames Cell Events: 9313 mean_circ: 0.927631
Ran for 463550 frames Cell Events: 9325 mean_circ: 0.927632
Ran for 464050 frames Cell Events: 9338 mean_circ: 0.927592
Ran for 464550 frames Cell Events: 9352 mean_circ: 0.927583
Ran for 465050 frames Cell Events: 9369 mean_circ: 0.927541
Ran for 465550 frames Cell Events: 9382 mean_circ: 0.92753
Ran for 466050 frames Cell Events: 9391 mean_circ: 0.92753
Ran for 467050 frames Cell Events: 9412 mean_circ: 0.927538
Ran for 467550 frames Cell Events: 9416 mean_circ: 0.927538
Ran for 468550 frames Cell Events: 9434 mean_circ: 0.927568
Ran for 469050 frames Cell Events: 9440 mean_circ: 0.927562
Ran for 469550 frames Cell Events: 9445 mean_circ: 0.927558
Ran for 470050 frames Cell Events: 9464 mean_circ: 0.927554
Ran for 471050 frames Cell Events: 9493 mean_circ: 0.927556
Ran for 471550 frames Cell Events: 9506 mean_circ: 0.92756
Ran for 472050 frames Cell Events: 9514 mean_circ: 0.927568
Ran for 472550 frames Cell Events: 9525 mean_circ: 0.927586
Ran for 473050 frames Cell Events: 9538 mean_circ: 0.92759
Ran for 473550 frames Cell Events: 9553 mean_circ: 0.927585
Ran for 474050 frames Cell Events: 9564 mean_circ: 0.927607
Ran for 474550 frames Cell Events: 9573 mean_circ: 0.927615
Ran for 475050 frames Cell Events: 9579 mean_circ: 0.927632
Ran for 475550 frames Cell Events: 9584 mean_circ: 0.927629
Ran for 477050 frames Cell Events: 9611 mean_circ: 0.927664
Ran for 478050 frames Cell Events: 9634 mean_circ: 0.927642
Ran for 479050 frames Cell Events: 9661 mean_circ: 0.927647
Ran for 479550 frames Cell Events: 9672 mean_circ: 0.927642
Ran for 480050 frames Cell Events: 9684 mean_circ: 0.927635
Ran for 480550 frames Cell Events: 9703 mean_circ: 0.927609
Ran for 481050 frames Cell Events: 9714 mean_circ: 0.927604
Ran for 481550 frames Cell Events: 9723 mean_circ: 0.927574
Ran for 482050 frames Cell Events: 9734 mean_circ: 0.927583
Ran for 482550 frames Cell Events: 9743 mean_circ: 0.927583
Ran for 483050 frames Cell Events: 9753 mean_circ: 0.927563
Ran for 483550 frames Cell Events: 9761 mean_circ: 0.927586
Ran for 484050 frames Cell Events: 9770 mean_circ: 0.927557
Ran for 484550 frames Cell Events: 9776 mean_circ: 0.927535
Ran for 485050 frames Cell Events: 9786 mean_circ: 0.927535
Ran for 485550 frames Cell Events: 9791 mean_circ: 0.92754
Ran for 486050 frames Cell Events: 9805 mean_circ: 0.927543
Ran for 486550 frames Cell Events: 9817 mean_circ: 0.927539
Ran for 488050 frames Cell Events: 9844 mean_circ: 0.92752
Ran for 488550 frames Cell Events: 9852 mean_circ: 0.927518
Ran for 489050 frames Cell Events: 9857 mean_circ: 0.927498
Ran for 489550 frames Cell Events: 9864 mean_circ: 0.927502
Ran for 490050 frames Cell Events: 9876 mean_circ: 0.927482
Ran for 490550 frames Cell Events: 9882 mean_circ: 0.927475
Ran for 491050 frames Cell Events: 9895 mean_circ: 0.927487
Ran for 491550 frames Cell Events: 9900 mean_circ: 0.92748
Ran for 492550 frames Cell Events: 9918 mean_circ: 0.927461
Ran for 493550 frames Cell Events: 9943 mean_circ: 0.927439
Ran for 494050 frames Cell Events: 9958 mean_circ: 0.927436
Ran for 494550 frames Cell Events: 9970 mean_circ: 0.927454
Ran for 495050 frames Cell Events: 9985 mean_circ: 0.92747
Ran for 496050 frames Cell Events: 9995 mean_circ: 0.927456
End this measurment.
because stop condition (time/nr of cells) was met
Stopping time: 3.7589e+008
****************************************
Measurement finished
Mean Circularitiy: 0.927466
Mean Area: 794.525
Mean FL-1 intensity: 3297.17
Mean FL-2 intensity: 4258.17
Mean FL-3 intensity: 0
frames processed: 494795
Total Time: 248232ms
Number of Runs (new bg img) 0
Frames collected: 496316
Skipped frames: 66485
Rescued Frames: 3246983
Samples DAQ: 124079075
Samples written: 248158032
Samples per Channel: 124079016
FPS: 1999.41
Samples per second: 499852
Expected samples/s: 500000
Events: 10000
Events per second: 40.285
jumped back (PMT): 9019
#Frames read, frequency
# 0 frames :625921
# 1 frames :338598
# 2 frames :9873
# 3 frames :2444
# 4 frames :1496
# 5 frames :484
# 6 frames :2299
# 7 frames :5098
# 8 frames :1869
# 9 frames :1047
# 10 frames :571
# 11 frames :641
# 12 frames :567
# 13 frames :550
# 14 frames :251
# 15 frames :226
# 16 frames :135
# 17 frames :99
# 18 frames :83
# 19 frames :64
# 20 frames :62
# 21 frames :62
# 22 frames :41
# 23 frames :19
# 24 frames :25
# 25 frames :18
# 26 frames :11
# 27 frames :10
# 28 frames :10
# 29 frames :6
# 30 frames :8
# 31 frames :3
# 32 frames :1
# 33 frames :5
# 34 frames :4
# 35 frames :1
# 46 frames :1
M1_para.ini
[General]Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.5874
parameters.txt
#fRT-DC Configuration file# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0
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Additional Information
Field | Value |
---|---|
license | Creative Commons Public Domain Dedication |
created | 3 months ago |
etag | 7b0529fb40332e4c7d4ca5dd8871e9fd |
format | RT-FDC |
id | 24b98f98-52bb-47c4-9739-4154c1c4f6a5 |
metadata modified | 3 months ago |
mimetype | RT-DC |
package id | 6559e138-d11d-46c2-926c-cbbedb60ee98 |
position | 0 |
s3 available | True |
s3 url | https://objectstore.hpccloud.mpcdf.mpg.de/circle-5d2846fc-f12d-45de-96d1-e1eea06e16c7/resource/24b/98f/98-52bb-47c4-9739-4154c1c4f6a5 |
sha256 | f7e794e7816594c5487456979d7d011f2092fe3f66f3c8e9887e8460213ca400 |
size | 175.5 MiB |
dc:experiment:date | 2017-05-19 |
dc:experiment:event count | 9,998 |
dc:experiment:run identifier | None-a3fb |
dc:experiment:run index | 1 |
dc:experiment:sample | 20170519_1643_dsRNA_M1_col |
dc:experiment:time | 16:43:24 |
dc:fluorescence:bit depth | 16 |
dc:fluorescence:channel 1 name | FL1 |
dc:fluorescence:channel 2 name | FL2 |
dc:fluorescence:channel count | 2 |
dc:fluorescence:channels installed | 3 |
dc:fluorescence:laser 1 lambda | 488 |
dc:fluorescence:laser 1 power | 10 |
dc:fluorescence:laser 2 lambda | 561 |
dc:fluorescence:laser 2 power | 35 |
dc:fluorescence:laser 3 lambda | 640 |
dc:fluorescence:laser 3 power | 0 |
dc:fluorescence:laser count | 2 |
dc:fluorescence:lasers installed | 3 |
dc:fluorescence:sample rate | 500,000 |
dc:fluorescence:samples per event | 300 |
dc:fluorescence:signal max | 1 |
dc:fluorescence:signal min | -1 |
dc:fluorescence:trace median | 5 |
dc:imaging:flash device | LED (undefined) |
dc:imaging:flash duration | 2 |
dc:imaging:frame rate | 2,000 |
dc:imaging:pixel size | 0.34 |
dc:imaging:roi position x | 504 |
dc:imaging:roi position y | 472 |
dc:imaging:roi size x | 256 |
dc:imaging:roi size y | 96 |
dc:online contour:bin area min | 50 |
dc:online contour:bin kernel | 5 |
dc:online contour:bin threshold | 6 |
dc:online contour:image blur | 0 |
dc:online contour:no absdiff | True |
dc:setup:channel width | 20 |
dc:setup:chip region | channel |
dc:setup:flow rate | 0.04 |
dc:setup:module composition | Cell_Flow_2, Fluor |
dc:setup:software version | fRT-DC V1.06170206 | dclab 0.60.3 |
sp:cells:cell type | KC167 |
sp:cells:live | True |
sp:cells:organism | Drosophila melanogaster |
sp:chip:channel width | 20 |
sp:experiment:buffer osmolality | 325 |
sp:experiment:buffer ph | 6.8 |
sp:experiment:concentration | 5 |
sp:identifiers:labels | GFP tdTomato |
sp:identifiers:tags | NLS-GFP |