Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_16.25.37
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "72901bd8eaf97513f0378d103b87bbb0",
"name": "M1_20170611_1931_dsRNA_P1_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "14:25:58"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8523 (max 8524) in '8523'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.2852
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.3729e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 13 mean_circ: 0.964198
Ran for 1050 frames Cell Events: 33 mean_circ: 0.951922
Ran for 1550 frames Cell Events: 43 mean_circ: 0.943998
Ran for 2550 frames Cell Events: 82 mean_circ: 0.940947
Ran for 3050 frames Cell Events: 91 mean_circ: 0.942596
Ran for 3550 frames Cell Events: 98 mean_circ: 0.943784
Ran for 4050 frames Cell Events: 117 mean_circ: 0.943257
Ran for 4550 frames Cell Events: 142 mean_circ: 0.94249
Ran for 5050 frames Cell Events: 156 mean_circ: 0.943387
Ran for 5550 frames Cell Events: 177 mean_circ: 0.943999
Ran for 6050 frames Cell Events: 188 mean_circ: 0.945545
Ran for 6550 frames Cell Events: 204 mean_circ: 0.946437
Ran for 7050 frames Cell Events: 211 mean_circ: 0.946665
Ran for 7550 frames Cell Events: 234 mean_circ: 0.946258
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Ran for 12550 frames Cell Events: 394 mean_circ: 0.944578
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Ran for 13550 frames Cell Events: 428 mean_circ: 0.94347
Ran for 14050 frames Cell Events: 437 mean_circ: 0.94356
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Ran for 30050 frames Cell Events: 1000 mean_circ: 0.945075
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Ran for 32550 frames Cell Events: 1095 mean_circ: 0.945257
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Ran for 67050 frames Cell Events: 2327 mean_circ: 0.944882
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Ran for 76550 frames Cell Events: 2665 mean_circ: 0.944995
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Ran for 81550 frames Cell Events: 2860 mean_circ: 0.945036
Ran for 82050 frames Cell Events: 2883 mean_circ: 0.945142
Ran for 83550 frames Cell Events: 2945 mean_circ: 0.945261
Ran for 84050 frames Cell Events: 2964 mean_circ: 0.945329
Ran for 84550 frames Cell Events: 2998 mean_circ: 0.945341
Ran for 85050 frames Cell Events: 3012 mean_circ: 0.945356
Ran for 85550 frames Cell Events: 3028 mean_circ: 0.945252
Ran for 86050 frames Cell Events: 3055 mean_circ: 0.94521
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Ran for 90050 frames Cell Events: 3202 mean_circ: 0.945193
Ran for 90550 frames Cell Events: 3220 mean_circ: 0.945193
Ran for 91550 frames Cell Events: 3260 mean_circ: 0.945291
Ran for 92050 frames Cell Events: 3282 mean_circ: 0.945304
Ran for 93050 frames Cell Events: 3325 mean_circ: 0.945294
Ran for 94050 frames Cell Events: 3366 mean_circ: 0.945344
Ran for 94550 frames Cell Events: 3385 mean_circ: 0.945407
Ran for 95050 frames Cell Events: 3405 mean_circ: 0.94543
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Ran for 96550 frames Cell Events: 3466 mean_circ: 0.945438
Ran for 97050 frames Cell Events: 3478 mean_circ: 0.945455
Ran for 97550 frames Cell Events: 3492 mean_circ: 0.945469
Ran for 98550 frames Cell Events: 3539 mean_circ: 0.945555
Ran for 99050 frames Cell Events: 3557 mean_circ: 0.945469
Ran for 100050 frames Cell Events: 3593 mean_circ: 0.945443
Ran for 100550 frames Cell Events: 3613 mean_circ: 0.945452
Ran for 101550 frames Cell Events: 3654 mean_circ: 0.945486
Ran for 102050 frames Cell Events: 3670 mean_circ: 0.945542
Ran for 102550 frames Cell Events: 3686 mean_circ: 0.945553
Ran for 103050 frames Cell Events: 3719 mean_circ: 0.945513
Ran for 104050 frames Cell Events: 3780 mean_circ: 0.94548
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Ran for 105550 frames Cell Events: 3832 mean_circ: 0.945381
Ran for 106050 frames Cell Events: 3851 mean_circ: 0.945327
Ran for 107050 frames Cell Events: 3896 mean_circ: 0.945353
Ran for 107550 frames Cell Events: 3922 mean_circ: 0.945286
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Ran for 118050 frames Cell Events: 4324 mean_circ: 0.945224
Ran for 118550 frames Cell Events: 4347 mean_circ: 0.945136
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Ran for 122050 frames Cell Events: 4484 mean_circ: 0.945247
Ran for 122550 frames Cell Events: 4499 mean_circ: 0.945273
Ran for 123050 frames Cell Events: 4520 mean_circ: 0.945292
Ran for 123550 frames Cell Events: 4540 mean_circ: 0.945339
Ran for 124550 frames Cell Events: 4581 mean_circ: 0.94538
Ran for 125550 frames Cell Events: 4632 mean_circ: 0.945383
Ran for 126050 frames Cell Events: 4652 mean_circ: 0.945426
Ran for 127550 frames Cell Events: 4702 mean_circ: 0.945493
Ran for 128050 frames Cell Events: 4723 mean_circ: 0.945493
Ran for 128550 frames Cell Events: 4743 mean_circ: 0.945433
Ran for 129050 frames Cell Events: 4762 mean_circ: 0.945458
Ran for 129550 frames Cell Events: 4787 mean_circ: 0.945484
Ran for 131050 frames Cell Events: 4845 mean_circ: 0.945488
Ran for 131550 frames Cell Events: 4864 mean_circ: 0.945477
Ran for 133050 frames Cell Events: 4934 mean_circ: 0.945556
Ran for 134550 frames Cell Events: 4982 mean_circ: 0.945508
Ran for 135050 frames Cell Events: 4999 mean_circ: 0.945517
Ran for 136550 frames Cell Events: 5057 mean_circ: 0.945579
Ran for 137050 frames Cell Events: 5080 mean_circ: 0.945642
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Ran for 143050 frames Cell Events: 5317 mean_circ: 0.945671
Ran for 143550 frames Cell Events: 5339 mean_circ: 0.945668
Ran for 144050 frames Cell Events: 5357 mean_circ: 0.945738
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Ran for 146550 frames Cell Events: 5455 mean_circ: 0.945626
Ran for 147050 frames Cell Events: 5469 mean_circ: 0.945649
Ran for 147550 frames Cell Events: 5492 mean_circ: 0.945612
Ran for 148550 frames Cell Events: 5525 mean_circ: 0.945663
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Ran for 152550 frames Cell Events: 5699 mean_circ: 0.945482
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Ran for 153550 frames Cell Events: 5734 mean_circ: 0.945531
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Ran for 171550 frames Cell Events: 6424 mean_circ: 0.94561
Ran for 172050 frames Cell Events: 6438 mean_circ: 0.945611
Ran for 172550 frames Cell Events: 6453 mean_circ: 0.945649
Ran for 173050 frames Cell Events: 6467 mean_circ: 0.945665
Ran for 173550 frames Cell Events: 6493 mean_circ: 0.945707
Ran for 174050 frames Cell Events: 6515 mean_circ: 0.945722
Ran for 174550 frames Cell Events: 6528 mean_circ: 0.945749
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Ran for 177550 frames Cell Events: 6616 mean_circ: 0.945723
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Ran for 179050 frames Cell Events: 6663 mean_circ: 0.945771
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Ran for 193550 frames Cell Events: 7169 mean_circ: 0.945862
Ran for 194050 frames Cell Events: 7186 mean_circ: 0.945856
Ran for 194550 frames Cell Events: 7195 mean_circ: 0.945827
Ran for 195050 frames Cell Events: 7217 mean_circ: 0.945821
Ran for 195550 frames Cell Events: 7236 mean_circ: 0.945809
Ran for 196550 frames Cell Events: 7271 mean_circ: 0.945851
Ran for 197050 frames Cell Events: 7288 mean_circ: 0.945894
Ran for 198550 frames Cell Events: 7355 mean_circ: 0.945884
Ran for 199050 frames Cell Events: 7372 mean_circ: 0.945888
Ran for 199550 frames Cell Events: 7395 mean_circ: 0.9459
Ran for 200050 frames Cell Events: 7411 mean_circ: 0.945905
Ran for 200550 frames Cell Events: 7425 mean_circ: 0.945889
Ran for 202050 frames Cell Events: 7483 mean_circ: 0.945893
Ran for 202550 frames Cell Events: 7503 mean_circ: 0.945874
Ran for 203050 frames Cell Events: 7512 mean_circ: 0.945878
Ran for 204550 frames Cell Events: 7557 mean_circ: 0.945865
Ran for 205550 frames Cell Events: 7598 mean_circ: 0.945907
Ran for 206050 frames Cell Events: 7614 mean_circ: 0.945913
Ran for 206550 frames Cell Events: 7631 mean_circ: 0.945936
Ran for 207050 frames Cell Events: 7647 mean_circ: 0.945945
Ran for 207550 frames Cell Events: 7674 mean_circ: 0.945959
Ran for 208550 frames Cell Events: 7697 mean_circ: 0.945942
Ran for 210050 frames Cell Events: 7755 mean_circ: 0.945908
Ran for 210550 frames Cell Events: 7773 mean_circ: 0.945924
Ran for 211050 frames Cell Events: 7801 mean_circ: 0.94595
Ran for 212050 frames Cell Events: 7844 mean_circ: 0.945964
Ran for 212550 frames Cell Events: 7862 mean_circ: 0.945969
Ran for 213050 frames Cell Events: 7871 mean_circ: 0.945967
Ran for 213550 frames Cell Events: 7881 mean_circ: 0.94597
Ran for 214050 frames Cell Events: 7898 mean_circ: 0.945986
Ran for 215050 frames Cell Events: 7937 mean_circ: 0.945976
Ran for 216050 frames Cell Events: 7981 mean_circ: 0.94599
Ran for 216550 frames Cell Events: 8003 mean_circ: 0.94596
Ran for 217050 frames Cell Events: 8032 mean_circ: 0.945938
Ran for 217550 frames Cell Events: 8055 mean_circ: 0.94594
Ran for 218050 frames Cell Events: 8073 mean_circ: 0.945896
Ran for 218550 frames Cell Events: 8093 mean_circ: 0.945907
Ran for 219050 frames Cell Events: 8116 mean_circ: 0.945854
Ran for 219550 frames Cell Events: 8131 mean_circ: 0.945868
Ran for 220050 frames Cell Events: 8166 mean_circ: 0.945868
Ran for 221050 frames Cell Events: 8218 mean_circ: 0.945817
Ran for 221550 frames Cell Events: 8243 mean_circ: 0.945858
Ran for 222550 frames Cell Events: 8300 mean_circ: 0.94586
Ran for 223550 frames Cell Events: 8348 mean_circ: 0.945911
Ran for 224050 frames Cell Events: 8368 mean_circ: 0.945927
Ran for 224550 frames Cell Events: 8389 mean_circ: 0.945908
Ran for 225050 frames Cell Events: 8401 mean_circ: 0.945886
Ran for 225550 frames Cell Events: 8425 mean_circ: 0.94587
End this measurment..
Stopping time: 2.37301e+009
****************************************
Measurement finished
Mean Circularitiy: 0.945863
Mean Area: 628.225
Mean FL-1 intensity: 3164.82
Mean FL-2 intensity: 4069.97
Mean FL-3 intensity: 0
frames processed: 227958
Total Time: 114089ms
Number of Runs (new bg img) 0
Frames collected: 228010
Skipped frames: 76767
Rescued Frames: 6804179
Samples DAQ: 57004492
Samples written: 114005104
Samples per Channel: 57002552
FPS: 1998.53
Samples per second: 499633
Expected samples/s: 500000
Events: 8524
Events per second: 74.7137
jumped back (PMT): 5287
#Frames read, frequency
# 0 frames :325139
# 1 frames :113012
# 2 frames :5394
# 3 frames :1669
# 4 frames :976
# 5 frames :438
# 6 frames :1733
# 7 frames :1910
# 8 frames :1138
# 9 frames :658
# 10 frames :999
# 11 frames :490
# 12 frames :496
# 13 frames :289
# 14 frames :305
# 15 frames :286
# 16 frames :251
# 17 frames :170
# 18 frames :132
# 19 frames :95
# 20 frames :74
# 21 frames :58
# 22 frames :47
# 23 frames :41
# 24 frames :29
# 25 frames :21
# 26 frames :27
# 27 frames :16
# 28 frames :13
# 29 frames :12
# 30 frames :12
# 31 frames :5
# 32 frames :9
# 33 frames :6
# 34 frames :4
# 35 frames :5
# 36 frames :2
# 37 frames :2
# 38 frames :2
# 39 frames :1
# 40 frames :2
# 42 frames :1
# 43 frames :1
# 47 frames :1
# 52 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.2852
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0