Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_18.46.26
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "81cf41da4c1f3e83e0f31728b09a2831",
"name": "M1_20170618_1528_dsRNA_Q8_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "16:46:49"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8840 (max 8841) in '8840'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9204
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.89854e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 2050 frames Cell Events: 54 mean_circ: 0.951531
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Ran for 193050 frames Cell Events: 6221 mean_circ: 0.951757
Ran for 193550 frames Cell Events: 6232 mean_circ: 0.951761
Ran for 194050 frames Cell Events: 6248 mean_circ: 0.951775
Ran for 194550 frames Cell Events: 6268 mean_circ: 0.951786
Ran for 195050 frames Cell Events: 6286 mean_circ: 0.951795
Ran for 195550 frames Cell Events: 6302 mean_circ: 0.951786
Ran for 196550 frames Cell Events: 6338 mean_circ: 0.951768
Ran for 197050 frames Cell Events: 6347 mean_circ: 0.951784
Ran for 198050 frames Cell Events: 6379 mean_circ: 0.95174
Ran for 198550 frames Cell Events: 6390 mean_circ: 0.951685
Ran for 200050 frames Cell Events: 6443 mean_circ: 0.951741
Ran for 200550 frames Cell Events: 6461 mean_circ: 0.951727
Ran for 201050 frames Cell Events: 6478 mean_circ: 0.951744
Ran for 201550 frames Cell Events: 6502 mean_circ: 0.951756
Ran for 202050 frames Cell Events: 6510 mean_circ: 0.951772
Ran for 202550 frames Cell Events: 6547 mean_circ: 0.951761
Ran for 203550 frames Cell Events: 6594 mean_circ: 0.951685
Ran for 204050 frames Cell Events: 6608 mean_circ: 0.951703
Ran for 205050 frames Cell Events: 6635 mean_circ: 0.951672
Ran for 206050 frames Cell Events: 6670 mean_circ: 0.951688
Ran for 206550 frames Cell Events: 6682 mean_circ: 0.951696
Ran for 207050 frames Cell Events: 6709 mean_circ: 0.951703
Ran for 208550 frames Cell Events: 6773 mean_circ: 0.951712
Ran for 210550 frames Cell Events: 6833 mean_circ: 0.951697
Ran for 211050 frames Cell Events: 6848 mean_circ: 0.951706
Ran for 212050 frames Cell Events: 6895 mean_circ: 0.951593
Ran for 212550 frames Cell Events: 6924 mean_circ: 0.951551
Ran for 213050 frames Cell Events: 6939 mean_circ: 0.951503
Ran for 213550 frames Cell Events: 6954 mean_circ: 0.951477
Ran for 214050 frames Cell Events: 6972 mean_circ: 0.951491
Ran for 215050 frames Cell Events: 7003 mean_circ: 0.951404
Ran for 215550 frames Cell Events: 7025 mean_circ: 0.951374
Ran for 216550 frames Cell Events: 7057 mean_circ: 0.951355
Ran for 217050 frames Cell Events: 7072 mean_circ: 0.951316
Ran for 217550 frames Cell Events: 7094 mean_circ: 0.951323
Ran for 218050 frames Cell Events: 7110 mean_circ: 0.951322
Ran for 219050 frames Cell Events: 7156 mean_circ: 0.951349
Ran for 219550 frames Cell Events: 7166 mean_circ: 0.951346
Ran for 221050 frames Cell Events: 7217 mean_circ: 0.951414
Ran for 221550 frames Cell Events: 7234 mean_circ: 0.951423
Ran for 222050 frames Cell Events: 7252 mean_circ: 0.951426
Ran for 222550 frames Cell Events: 7267 mean_circ: 0.951412
Ran for 223050 frames Cell Events: 7284 mean_circ: 0.951411
Ran for 223550 frames Cell Events: 7299 mean_circ: 0.951421
Ran for 224050 frames Cell Events: 7312 mean_circ: 0.95138
Ran for 224550 frames Cell Events: 7321 mean_circ: 0.951397
Ran for 225050 frames Cell Events: 7339 mean_circ: 0.95142
Ran for 225550 frames Cell Events: 7355 mean_circ: 0.951403
Ran for 226050 frames Cell Events: 7375 mean_circ: 0.951351
Ran for 227050 frames Cell Events: 7405 mean_circ: 0.951333
Ran for 227550 frames Cell Events: 7429 mean_circ: 0.951309
Ran for 228050 frames Cell Events: 7451 mean_circ: 0.951325
Ran for 229050 frames Cell Events: 7470 mean_circ: 0.95132
Ran for 229550 frames Cell Events: 7482 mean_circ: 0.951323
Ran for 230050 frames Cell Events: 7496 mean_circ: 0.951321
Ran for 230550 frames Cell Events: 7516 mean_circ: 0.951321
Ran for 231550 frames Cell Events: 7543 mean_circ: 0.951339
Ran for 232050 frames Cell Events: 7567 mean_circ: 0.951328
Ran for 233050 frames Cell Events: 7603 mean_circ: 0.951353
Ran for 233550 frames Cell Events: 7619 mean_circ: 0.951314
Ran for 236550 frames Cell Events: 7728 mean_circ: 0.951259
Ran for 237050 frames Cell Events: 7735 mean_circ: 0.951271
Ran for 238050 frames Cell Events: 7773 mean_circ: 0.951256
Ran for 238550 frames Cell Events: 7795 mean_circ: 0.951257
Ran for 239050 frames Cell Events: 7809 mean_circ: 0.951273
Ran for 239550 frames Cell Events: 7817 mean_circ: 0.951271
Ran for 241050 frames Cell Events: 7875 mean_circ: 0.951286
Ran for 241550 frames Cell Events: 7894 mean_circ: 0.951276
Ran for 242050 frames Cell Events: 7915 mean_circ: 0.951293
Ran for 242550 frames Cell Events: 7938 mean_circ: 0.951302
Ran for 245050 frames Cell Events: 8034 mean_circ: 0.951279
Ran for 245550 frames Cell Events: 8041 mean_circ: 0.951281
Ran for 246050 frames Cell Events: 8059 mean_circ: 0.95124
Ran for 248050 frames Cell Events: 8126 mean_circ: 0.951264
Ran for 248550 frames Cell Events: 8138 mean_circ: 0.951266
Ran for 249550 frames Cell Events: 8179 mean_circ: 0.951204
Ran for 250050 frames Cell Events: 8196 mean_circ: 0.951193
Ran for 250550 frames Cell Events: 8213 mean_circ: 0.951173
Ran for 251550 frames Cell Events: 8257 mean_circ: 0.951194
Ran for 252050 frames Cell Events: 8272 mean_circ: 0.95121
Ran for 252550 frames Cell Events: 8285 mean_circ: 0.95122
Ran for 253550 frames Cell Events: 8326 mean_circ: 0.951243
Ran for 254050 frames Cell Events: 8342 mean_circ: 0.95125
Ran for 255050 frames Cell Events: 8375 mean_circ: 0.951283
Ran for 255550 frames Cell Events: 8390 mean_circ: 0.951292
Ran for 256550 frames Cell Events: 8427 mean_circ: 0.9513
Ran for 257050 frames Cell Events: 8437 mean_circ: 0.951298
Ran for 257550 frames Cell Events: 8456 mean_circ: 0.9513
Ran for 258050 frames Cell Events: 8473 mean_circ: 0.951317
Ran for 259050 frames Cell Events: 8509 mean_circ: 0.95131
Ran for 259550 frames Cell Events: 8521 mean_circ: 0.951315
Ran for 260050 frames Cell Events: 8531 mean_circ: 0.951308
Ran for 261050 frames Cell Events: 8576 mean_circ: 0.951309
Ran for 261550 frames Cell Events: 8592 mean_circ: 0.951299
Ran for 262050 frames Cell Events: 8607 mean_circ: 0.9513
Ran for 262550 frames Cell Events: 8629 mean_circ: 0.951284
Ran for 263050 frames Cell Events: 8646 mean_circ: 0.951303
Ran for 264050 frames Cell Events: 8683 mean_circ: 0.951311
Ran for 265050 frames Cell Events: 8720 mean_circ: 0.951253
Ran for 265550 frames Cell Events: 8738 mean_circ: 0.951226
Ran for 266550 frames Cell Events: 8769 mean_circ: 0.951222
Ran for 267050 frames Cell Events: 8786 mean_circ: 0.951234
Ran for 267550 frames Cell Events: 8796 mean_circ: 0.951242
Ran for 268050 frames Cell Events: 8813 mean_circ: 0.95123
Ran for 269050 frames Cell Events: 8835 mean_circ: 0.951197
End this measurment..
Stopping time: 3.89989e+008
****************************************
Measurement finished
Mean Circularitiy: 0.951176
Mean Area: 592.944
Mean FL-1 intensity: 3012.11
Mean FL-2 intensity: 3571.67
Mean FL-3 intensity: 0
frames processed: 269229
Total Time: 134717ms
Number of Runs (new bg img) 0
Frames collected: 269282
Skipped frames: 15143
Rescued Frames: 2085124
Samples DAQ: 67320549
Samples written: 134641006
Samples per Channel: 67320503
FPS: 1998.88
Samples per second: 499719
Expected samples/s: 500000
Events: 8841
Events per second: 65.6266
jumped back (PMT): 6370
#Frames read, frequency
# 0 frames :375880
# 1 frames :142344
# 2 frames :6453
# 3 frames :2027
# 4 frames :1211
# 5 frames :464
# 6 frames :960
# 7 frames :3034
# 8 frames :1065
# 9 frames :730
# 10 frames :1186
# 11 frames :827
# 12 frames :478
# 13 frames :410
# 14 frames :227
# 15 frames :271
# 16 frames :187
# 17 frames :188
# 18 frames :118
# 19 frames :84
# 20 frames :46
# 21 frames :40
# 22 frames :55
# 23 frames :46
# 24 frames :34
# 25 frames :27
# 26 frames :27
# 27 frames :18
# 28 frames :25
# 29 frames :15
# 30 frames :12
# 31 frames :11
# 32 frames :9
# 33 frames :5
# 34 frames :5
# 35 frames :1
# 37 frames :3
# 39 frames :2
# 41 frames :2
# 53 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9204
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0