Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_19.02.05
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "f8458173b7eb8d56b8be3cfb57b58ed8",
"name": "M1_20170618_1909_dsRNA_S1_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:02:26"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8333 (max 8334) in '8333'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0399
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 4.0313e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 21 mean_circ: 0.955815
Ran for 2050 frames Cell Events: 55 mean_circ: 0.951677
Ran for 2550 frames Cell Events: 71 mean_circ: 0.94865
Ran for 3050 frames Cell Events: 88 mean_circ: 0.949103
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Ran for 5050 frames Cell Events: 139 mean_circ: 0.953372
Ran for 5550 frames Cell Events: 152 mean_circ: 0.952552
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Ran for 8050 frames Cell Events: 198 mean_circ: 0.953182
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Ran for 11550 frames Cell Events: 276 mean_circ: 0.954142
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Ran for 14050 frames Cell Events: 357 mean_circ: 0.953176
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Ran for 67050 frames Cell Events: 1894 mean_circ: 0.950049
Ran for 68550 frames Cell Events: 1957 mean_circ: 0.950007
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Ran for 82550 frames Cell Events: 2389 mean_circ: 0.949763
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Ran for 83550 frames Cell Events: 2424 mean_circ: 0.949668
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Ran for 91050 frames Cell Events: 2669 mean_circ: 0.949522
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Ran for 94050 frames Cell Events: 2769 mean_circ: 0.949484
Ran for 95550 frames Cell Events: 2803 mean_circ: 0.949589
Ran for 96550 frames Cell Events: 2837 mean_circ: 0.949535
Ran for 97550 frames Cell Events: 2868 mean_circ: 0.949548
Ran for 98050 frames Cell Events: 2883 mean_circ: 0.949509
Ran for 99550 frames Cell Events: 2933 mean_circ: 0.949523
Ran for 100050 frames Cell Events: 2950 mean_circ: 0.949511
Ran for 102550 frames Cell Events: 3030 mean_circ: 0.949615
Ran for 103550 frames Cell Events: 3068 mean_circ: 0.94966
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Ran for 106050 frames Cell Events: 3153 mean_circ: 0.949737
Ran for 107050 frames Cell Events: 3179 mean_circ: 0.949746
Ran for 107550 frames Cell Events: 3192 mean_circ: 0.949699
Ran for 108050 frames Cell Events: 3217 mean_circ: 0.949774
Ran for 108550 frames Cell Events: 3235 mean_circ: 0.949806
Ran for 109050 frames Cell Events: 3254 mean_circ: 0.949845
Ran for 110050 frames Cell Events: 3277 mean_circ: 0.949807
Ran for 110550 frames Cell Events: 3291 mean_circ: 0.949816
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Ran for 112050 frames Cell Events: 3330 mean_circ: 0.949829
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Ran for 122550 frames Cell Events: 3680 mean_circ: 0.949773
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Ran for 125050 frames Cell Events: 3765 mean_circ: 0.949849
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Ran for 126050 frames Cell Events: 3796 mean_circ: 0.949893
Ran for 127050 frames Cell Events: 3836 mean_circ: 0.949967
Ran for 127550 frames Cell Events: 3852 mean_circ: 0.949876
Ran for 128050 frames Cell Events: 3872 mean_circ: 0.949867
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Ran for 132050 frames Cell Events: 3996 mean_circ: 0.949844
Ran for 132550 frames Cell Events: 4014 mean_circ: 0.949872
Ran for 133550 frames Cell Events: 4046 mean_circ: 0.949926
Ran for 134050 frames Cell Events: 4058 mean_circ: 0.94996
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Ran for 143550 frames Cell Events: 4313 mean_circ: 0.949829
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Ran for 146550 frames Cell Events: 4421 mean_circ: 0.949817
Ran for 147050 frames Cell Events: 4433 mean_circ: 0.949843
Ran for 149050 frames Cell Events: 4510 mean_circ: 0.949871
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Ran for 152550 frames Cell Events: 4604 mean_circ: 0.94995
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Ran for 154050 frames Cell Events: 4652 mean_circ: 0.949959
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Ran for 156550 frames Cell Events: 4749 mean_circ: 0.950101
Ran for 157550 frames Cell Events: 4777 mean_circ: 0.9501
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Ran for 202050 frames Cell Events: 6240 mean_circ: 0.950425
Ran for 203550 frames Cell Events: 6282 mean_circ: 0.950403
Ran for 206050 frames Cell Events: 6353 mean_circ: 0.950379
Ran for 206550 frames Cell Events: 6369 mean_circ: 0.950396
Ran for 207050 frames Cell Events: 6379 mean_circ: 0.950412
Ran for 207550 frames Cell Events: 6391 mean_circ: 0.950401
Ran for 208550 frames Cell Events: 6424 mean_circ: 0.950382
Ran for 209050 frames Cell Events: 6440 mean_circ: 0.950368
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Ran for 220050 frames Cell Events: 6813 mean_circ: 0.95039
Ran for 220550 frames Cell Events: 6821 mean_circ: 0.950373
Ran for 221050 frames Cell Events: 6838 mean_circ: 0.950352
Ran for 221550 frames Cell Events: 6855 mean_circ: 0.950366
Ran for 222050 frames Cell Events: 6867 mean_circ: 0.950362
Ran for 222550 frames Cell Events: 6882 mean_circ: 0.950361
Ran for 223050 frames Cell Events: 6892 mean_circ: 0.950368
Ran for 223550 frames Cell Events: 6913 mean_circ: 0.95037
Ran for 224050 frames Cell Events: 6930 mean_circ: 0.950367
Ran for 225050 frames Cell Events: 6974 mean_circ: 0.950364
Ran for 225550 frames Cell Events: 6993 mean_circ: 0.950381
Ran for 227550 frames Cell Events: 7058 mean_circ: 0.950405
Ran for 229050 frames Cell Events: 7113 mean_circ: 0.950451
Ran for 230050 frames Cell Events: 7148 mean_circ: 0.950432
Ran for 230550 frames Cell Events: 7157 mean_circ: 0.95044
Ran for 231050 frames Cell Events: 7175 mean_circ: 0.950422
Ran for 231550 frames Cell Events: 7200 mean_circ: 0.950422
Ran for 232550 frames Cell Events: 7235 mean_circ: 0.950384
Ran for 233550 frames Cell Events: 7265 mean_circ: 0.950389
Ran for 234050 frames Cell Events: 7293 mean_circ: 0.950395
Ran for 234550 frames Cell Events: 7307 mean_circ: 0.950388
Ran for 235050 frames Cell Events: 7320 mean_circ: 0.950398
Ran for 235550 frames Cell Events: 7334 mean_circ: 0.950338
Ran for 237050 frames Cell Events: 7392 mean_circ: 0.950291
Ran for 237550 frames Cell Events: 7411 mean_circ: 0.950302
Ran for 238050 frames Cell Events: 7420 mean_circ: 0.950264
Ran for 239050 frames Cell Events: 7450 mean_circ: 0.950243
Ran for 239550 frames Cell Events: 7467 mean_circ: 0.950258
Ran for 240550 frames Cell Events: 7499 mean_circ: 0.950281
Ran for 241050 frames Cell Events: 7516 mean_circ: 0.950302
Ran for 242050 frames Cell Events: 7552 mean_circ: 0.950309
Ran for 242550 frames Cell Events: 7570 mean_circ: 0.950328
Ran for 243550 frames Cell Events: 7618 mean_circ: 0.950263
Ran for 244050 frames Cell Events: 7631 mean_circ: 0.950256
Ran for 244550 frames Cell Events: 7641 mean_circ: 0.950271
Ran for 245050 frames Cell Events: 7665 mean_circ: 0.950299
Ran for 248050 frames Cell Events: 7787 mean_circ: 0.950237
Ran for 248550 frames Cell Events: 7806 mean_circ: 0.950214
Ran for 249050 frames Cell Events: 7815 mean_circ: 0.950207
Ran for 250050 frames Cell Events: 7857 mean_circ: 0.950178
Ran for 251550 frames Cell Events: 7912 mean_circ: 0.950219
Ran for 253550 frames Cell Events: 7979 mean_circ: 0.950223
Ran for 255050 frames Cell Events: 8058 mean_circ: 0.950204
Ran for 255550 frames Cell Events: 8080 mean_circ: 0.950183
Ran for 256050 frames Cell Events: 8103 mean_circ: 0.950188
Ran for 258550 frames Cell Events: 8208 mean_circ: 0.950173
Ran for 259050 frames Cell Events: 8223 mean_circ: 0.950134
Ran for 259550 frames Cell Events: 8248 mean_circ: 0.950136
Ran for 260050 frames Cell Events: 8264 mean_circ: 0.95013
Ran for 261550 frames Cell Events: 8331 mean_circ: 0.950102
End this measurment..
Stopping time: 4.03261e+008
****************************************
Measurement finished
Mean Circularitiy: 0.950005
Mean Area: 611.598
Mean FL-1 intensity: 2926.88
Mean FL-2 intensity: 3391.77
Mean FL-3 intensity: 0
frames processed: 261087
Total Time: 130926ms
Number of Runs (new bg img) 0
Frames collected: 261696
Skipped frames: 11067
Rescued Frames: 1703170
Samples DAQ: 65423915
Samples written: 130847582
Samples per Channel: 65423791
FPS: 1998.81
Samples per second: 499700
Expected samples/s: 500000
Events: 8335
Events per second: 63.6619
jumped back (PMT): 5069
#Frames read, frequency
# 0 frames :385147
# 1 frames :118762
# 2 frames :4991
# 3 frames :2237
# 4 frames :1316
# 5 frames :848
# 6 frames :972
# 7 frames :1484
# 8 frames :1281
# 9 frames :958
# 10 frames :1059
# 11 frames :705
# 12 frames :653
# 13 frames :435
# 14 frames :344
# 15 frames :280
# 16 frames :287
# 17 frames :277
# 18 frames :211
# 19 frames :168
# 20 frames :150
# 21 frames :129
# 22 frames :101
# 23 frames :82
# 24 frames :69
# 25 frames :65
# 26 frames :45
# 27 frames :40
# 28 frames :40
# 29 frames :31
# 30 frames :28
# 31 frames :16
# 32 frames :29
# 33 frames :15
# 34 frames :14
# 35 frames :15
# 36 frames :6
# 37 frames :9
# 38 frames :10
# 39 frames :10
# 40 frames :6
# 41 frames :3
# 42 frames :5
# 43 frames :2
# 44 frames :6
# 45 frames :2
# 46 frames :1
# 47 frames :1
# 48 frames :2
# 49 frames :3
# 50 frames :2
# 51 frames :2
# 53 frames :2
# 54 frames :3
# 58 frames :1
# 59 frames :2
# 63 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0399
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0